$LOCUS pBI-EGFP 5121 bp DNA SYN 25-Mar-2008 DEFINITION pBI-EGFP ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5121 /organism="pBI-EGFP" /mol_type="other DNA" promoter complement(23..118) /label="CMV2_promoter" misc_feature complement(85..109) /label="LNCX_primer" misc_feature complement(92..111) /label="pCEP_fwd_primer" misc_feature 134..439 /label="TRE" misc_feature 471..490 /label="pCEP_fwd_primer" misc_feature 473..497 /label="LNCX_primer" promoter 440..559 /label="CMV2_promoter" misc_feature 658..1230 /label="bGlob_int" terminator 1282..1795 /label="rb_glob_PA_terminator" rep_origin complement(2029..2648) /label="pBR322_origin" CDS complement(2803..3663) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2803..3663) /label="Ampicillin" /gene="Ampicillin" promoter complement(3705..3733) /label="AmpR_promoter" terminator 4202..4333 /label="SV40_PA_terminator" misc_feature 4290..4309 /label="EBV_rev_primer" misc_feature complement(4415..4436) /label="EGFP_C_primer" CDS complement(4370..5089) /label="EGFP" /gene="EGFP" /note="ORF frame 2" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" gene complement(4373..5089) /label="EGFP" /gene="EGFP" misc_feature 5023..5044 /label="EGFP_N_primer" misc_feature complement(5097..5113) /label="pBluescriptSK_primer" ORIGIN 1 CTGCAGGAAT TCGGGGCCGC GGAGGCTGGA TCGGTCCCGG TGTCTTCTAT GGAGGTCAAA 61 ACAGCGTGGA TGGCGTCTCC AGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA 121 GGTCGAGTTT ACCACTCCCT ATCAGTGATA GAGAAAAGTG AAAGTCGAGT TTACCACTCC 181 CTATCAGTGA TAGAGAAAAG TGAAAGTCGA GTTTACCACT CCCTATCAGT GATAGAGAAA 241 AGTGAAAGTC GAGTTTACCA CTCCCTATCA GTGATAGAGA AAAGTGAAAG TCGAGTTTAC 301 CACTCCCTAT CAGTGATAGA GAAAAGTGAA AGTCGAGTTT ACCACTCCCT ATCAGTGATA 361 GAGAAAAGTG AAAGTCGAGT TTACCACTCC CTATCAGTGA TAGAGAAAAG TGAAAGTCGA 421 GCTCGGTACC CGGGTCGAGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT 481 TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC 541 ACCGGGACCG ATCCAGCCTC CGCGGCCCCG AATTCGAGCT CGGTACCCGG GGATCCTCTA 601 GTCAGCTGAC GCGTGCTAGC GCGGCCTCGA CGATATCTCT AGACTGAGAA CTTCAGGGTG 661 AGTTTGGGGA CCCTTGATTG TTCTTTCTTT TTCGCTATTG AAAAATTCAT GTTATATGGA 721 GGGGGCAAAG TTTTCAGGGT GTTGTTTAGA ATGGGAAGAT GTCCCTTGTA TCACCATGGA 781 CCCTCATGAT AATTTTGTTT CTTTCACTTT CTACTCTGTT GACAACCATT GTCTCCTCTT 841 ATTTTCTTTT CATTTTCTGT AACTTTTTTC GTTAAACTTT AGCTTGCATT TGTAACGAAT 901 TTTTAAATTC ACTTTCGTTT ATTTGTCAGA TTGTAAGTAC TTTCTCTAAT CACTTTTTTT 961 TCAAGGCAAT CAGGGTAATT ATATTGTACT TCAGCACAGT TTTAGAGAAC AATTGTTATA 1021 ATTAAATGAT AAGGTAGAAT ATTTCTGCAT ATAAATTCTG GCTGGCGTGG AAATATTCTT 1081 ATTGGTAGAA ACAACTACAT CCTGGTAATC ATCCTGCCTT TCTCTTTATG GTTACAATGA 1141 TATACACTGT TTGAGATGAG GATAAAATAC TCTGAGTCCA AACCGGGCCC CTCTGCTAAC 1201 CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG 1261 TCTCATCATT TTGGCAAAGA ATTCACTCCT CAGGTGCAGG CTGCCTATCA GAAGGTGGTG 1321 GCTGGTGTGG CCAATGCCCT GGCTCACAAA TACCACTGAG ATCTTTTTCC CTCTGCCAAA 1381 AATTATGGGG ACATCATGAA GCCCCTTGAG CATCTGACTT CTGGGTAATA AAGGAAATTT 1441 ATTTTCATTG CAATAGTGTG TGGGAATTTT TTGTGTCTCT CACTCGGAAG GACATATGGG 1501 AGGGCAAATC ATTTAAAACA TCAGAATGAG TATTTGGTTT AGAGTTTGGC AACATATGCC 1561 ATATGCTGGC TGCCATGAAC AAAGGTGGCT ATAAAGAGGT CATCAGTATA TGAAACAGCC 1621 CCCTGCTGTC CATTCCTTAT TCCATAGAAA AGCCTTGACT TGAGGTTAGA TTTTTTTTAT 1681 ATTTTGTTTT GTGTTATTTT TTTCTTTAAC ATCCCTAAAA TTTTCCTTAC ATGTTTTACT 1741 AGCCAGATTT TTCCTCCTCT CCTGACTACT CCCAGTCATA GCTGTCCCTC TTCTCTTATG 1801 AACTCGACTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 1861 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 1921 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 1981 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 2041 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 2101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 2161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 2221 AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 2281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 2341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 2401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 2461 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 2521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 2581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 2641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 2701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 2761 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 2821 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 2881 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2941 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 3001 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 3061 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 3121 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 3181 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 3241 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 3301 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 3361 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 3421 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 3481 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 3541 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 3601 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 3661 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 3721 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 3781 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 3841 GCGTATCACG AGGCCCTTTC GTCTTCACTC GACGCTGGTC GAGCTGATAC TTCCCGTCCG 3901 CCAGGGGACA TGCCGGCGAT GCTGAAGGTC GCGCGCATTC CCGATGAAGA GGCCGGTTAC 3961 CGCCTGTTGA CCTGGTGGGA CGGGCAGGGC GCCGCCCGAG TCTTGCCTCG GCGGCGGGCG 4021 CTCTGCTCAT GGAGCGCGCG TCCGGGGACC TTGCACAGAT AGCGTGGTCC GGCCAGACGA 4081 CGAGGCTTGC AGGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA 4141 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA 4201 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 4261 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 4321 TCATGTCTGG ATCCTCTAGA ACTAGGTCGA CAAGCTTGAT ATCGAATTCT TACTTGTACA 4381 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT 4441 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG 4501 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA 4561 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT 4621 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA 4681 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC 4741 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC 4801 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT 4861 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG 4921 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT 4981 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA 5041 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATC CGCGGGGATC 5101 CACTAGTTCT AGAGCGGCCG C //  pBI-EGFP "Times New Roman#CvCMV2_pro"Times New RomanCUmLNCX_pri"Times New RomanCɆ\o pCEP_fwd_pri"Times New Roman*TRE"Times New RomanCɆ/CMV2_pro"Times New RomanC pCEP_fwd_pri"Times New RomanCLNCX_pri"Times New Roman) bGlob_int"Times New RomanCrb_glob_PA_term"Times New RomanCX  pBR322_origin"Times New RomanCɆ OORF 1"Times New Roman O Ampicillin"Times New RomanCyAmpR_pro"Times New RomanCj SV40_PA_term"Times New RomanCɆ EBV_rev_pri"Times New RomanCORF 2"Times New RomanCEGFP"Times New Roman?T EGFP_C_pri"Times New Roman)CɆ EGFP_N_pri"Times New Roman)pBluescriptSK_pri"Times New Roman PstI"Times New Roman StuI(459),PvuII(605)"Times New Roman*44t NheI"Times New RomangBP]] NcoI"Times New Roman`p  ApaI(1189),MscI(1330)"Times New Roman~{l BglII"Times New RomanO FspI"Times New Roman Tdss AatII"Times New RomanV NarI"Times New Romanuqm^ft HpaI(4198),SalI(4346)"Times New Romanf;3*m9 HindIII"Times New Roman ~  SpeI(5102),NotI(5115),EagI(5115)"Times New RomanMf test"Times New Roman6 H test"Times New Romanct test"Times New Roman&  17