Sequence of pBI-EGFP

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Sequence of pBI-EGFP

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Sequence of pBI-EGFP : 5121 BP; 1213 A; 1244 C; 1257 G; 1407 T; 0 other;

PstI(5) .......... .......... .......... .......... ..........

1 ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa 60

1 gacgtcctta agccccggcg cctccgacct agccagggcc acagaagata cctccagttt 60

.......... .......... .......... .......... CMV2_pro ..........

.......... .......... .......... .......... .......... ..........

61 acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata 120

61 tgtcgcacct accgcagagg tccgctagac tgccaagtga tttgctcgag acgaatatat 120

.......... ......... CMV2_pro LNCX_pri pCEP_fwd_pri .........

.......... .......... .......... .......... .......... ..........

121 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc 180

121 ccagctcaaa tggtgaggga tagtcactat ctcttttcac tttcagctca aatggtgagg 180

.......... .......... .......... TRE .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa 240

181 gatagtcact atctcttttc actttcagct caaatggtga gggatagtca ctatctcttt 240

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac 300

241 tcactttcag ctcaaatggt gagggatagt cactatctct tttcactttc agctcaaatg 300

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata 360

301 gtgagggata gtcactatct cttttcactt tcagctcaaa tggtgaggga tagtcactat 360

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 420

361 ctcttttcac tttcagctca aatggtgagg gatagtcact atctcttttc actttcagct 420

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc ctatataagc agagctcgt t 480

421 cgagccatgg gcccagctca tccgcacatg ccaccctccg gatatattcg tctcgagcaa 480

TRE .......... .......... CMV2_pro ......... pCEP_fwd_pri

.......... .......... .......... .......... .......... ..........

481 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac 540

481 atcacttggc agtctagcgg acctctgcgg taggtgcgac aaaactggag gtatcttctg 540

pCEP_fwd_pri LNCX_pri CMV2_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta 600

541 tggccctggc taggtcggag gcgccggggc ttaagctcga gccatgggcc cctaggagat 600

CMV2_pro .......... .......... .......... .......... ..........

PvuII(605) NheI(615) .......... .......... .......... ..........

601 gtcagctgac gcgtgctagc gcggcctcga cgatatctct agactgagaa cttcagggtg 660

601 cagtcgactg cgcacgatcg cgccggagct gctatagaga tctgactctt gaagtcccac 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agtttgggga cccttgattg ttctttcttt ttcgctattg aaaaattcat gttatatgga 720

661 tcaaacccct gggaactaac aagaaagaaa aagcgataac tttttaagta caatatacct 720

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... NcoI(774)

721 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga 780

721 cccccgtttc aaaagtccca caacaaatct tacccttcta cagggaacat agtggtacct 780

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt 840

781 gggagtacta ttaaaacaaa gaaagtgaaa gatgagacaa ctgttggtaa cagaggagaa 840

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 attttctttt cattttctgt aacttttttc gttaaacttt agcttgcatt tgtaacgaat 900

841 taaaagaaaa gtaaaagaca ttgaaaaaag caatttgaaa tcgaacgtaa acattgctta 900

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttttaaattc actttcgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt 960

901 aaaatttaag tgaaagcaaa taaacagtct aacattcatg aaagagatta gtgaaaaaaa 960

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcaaggcaat cagggtaatt atattgtact tcagcacagt tttagagaac aattgttata 1020

961 agttccgtta gtcccattaa tataacatga agtcgtgtca aaatctcttg ttaacaatat 1020

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt 1080

1021 taatttacta ttccatctta taaagacgta tatttaagac cgaccgcacc tttataagaa 1080

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 attggtagaa acaactacat cctggtaatc atcctgcctt tctctttatg gttacaatga 1140

1081 taaccatctt tgttgatgta ggaccattag taggacggaa agagaaatac caatgttact 1140

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac 1200

1141 atatgtgaca aactctactc ctattttatg agactcaggt ttggcccggg gagacgattg 1200

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg 1260

1201 gtacaagtac ggaagaagaa aaaggatgtc gaggacccgt tgcacgacca acaacacgac 1260

.......... bGlob_int .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca gaaggtggtg 1320

1261 agagtagtaa aaccgtttct taagtgagga gtccacgtcc gacggatagt cttccaccac 1320

.......... .......... .......... ....... rb_glob_PA_term .......

MscI(1330) .......... ......... BglII(1359) ......... ..........

1321 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa 1380

1321 cgaccacacc ggttacggga ccgagtgttt atggtgactc tagaaaaagg gagacggttt 1380

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aattatgggg acatcatgaa gccccttgag catctgactt ctgggtaata aaggaaattt 1440

1381 ttaatacccc tgtagtactt cggggaactc gtagactgaa gacccattat ttcctttaaa 1440

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 attttcattg caatagtgtg tgggaatttt ttgtgtctct cactcggaag gacatatggg 1500

1441 taaaagtaac gttatcacac acccttaaaa aacacagaga gtgagccttc ctgtataccc 1500

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc 1560

1501 tcccgtttag taaattttgt agtcttactc ataaaccaaa tctcaaaccg ttgtatacgg 1560

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc 1620

1561 tatacgaccg acggtacttg tttccaccga tatttctcca gtagtcatat actttgtcgg 1620

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 1680

1621 gggacgacag gtaaggaata aggtatcttt tcggaactga actccaatct aaaaaaaata 1680

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 1740

1681 taaaacaaaa cacaataaaa aaagaaattg tagggatttt aaaaggaatg tacaaaatga 1740

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 1800

1741 tcggtctaaa aaggaggaga ggactgatga gggtcagtat cgacagggag aagagaatac 1800

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aactcgactg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 1860

1801 ttgagctgac gtaattactt agccggttgc gcgcccctct ccgccaaacg cataacccgc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 1920

1861 gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg ccgctcgcca 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 1980

1921 tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat tgcgtccttt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 2040

1981 cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc gcaacgaccg 2040

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2041 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 2100

2041 caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga gttcagtctc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 2160

2101 caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt cgagggagca 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 2220

2161 cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag agggaagccc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 2280

2221 ttcgcaccgc gaaagagtta cgagtgcgac atccatagag tcaagccaca tccagcaagc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 2340

2281 gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 2400

2341 attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 2460

2401 accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga acttcaccac 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 2520

2461 cggattgatg ccgatgtgat cttcctgtca taaaccatag acgcgagacg acttcggtca 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2580

2521 atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc gaccatcgcc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 2640

2581 accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag ttcttctagg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 2700

2641 aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 2760

2701 ccagtactct aatagttttt cctagaagtg gatctaggaa aatttaattt ttacttcaaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 2820

2761 atttagttag atttcatata tactcatttg aaccagactg tcaatggtta cgaattagtc 2820

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

2821 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 2880

2821 actccgtgga tagagtcgct agacagataa agcaagtagg tatcaacgga ctgaggggca 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 2940

2881 gcacatctat tgatgctatg ccctcccgaa tggtagaccg gggtcacgac gttactatgg 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 3000

2941 cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat ttggtcggtc ggccttcccg 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 3060

3001 gctcgcgtct tcaccaggac gttgaaatag gcggaggtag gtcagataat taacaacggc 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... FspI(3098) .......... ..........

3061 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 3120

3061 ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg ttgcaacaac ggtaacgatg 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 3180

3121 tccgtagcac cacagtgcga gcagcaaacc ataccgaagt aagtcgaggc caagggttgc 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 3240

3181 tagttccgct caatgtacta gggggtacaa cacgtttttt cgccaatcga ggaagccagg 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 3300

3241 aggctagcaa cagtcttcat tcaaccggcg tcacaatagt gagtaccaat accgtcgtga 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 3360

3301 cgtattaaga gaatgacagt acggtaggca ttctacgaaa agacactgac cactcatgag 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 3420

3361 ttggttcagt aagactctta tcacatacgc cgctggctca acgagaacgg gccgcagtta 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 3480

3421 tgccctatta tggcgcggtg tatcgtcttg aaattttcac gagtagtaac cttttgcaag 3480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 3540

3481 aagccccgct tttgagagtt cctagaatgg cgacaactct aggtcaagct acattgggtg 3540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 3600

3541 agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg tcgcaaagac ccactcgttt 3600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 3660

3601 ttgtccttcc gttttacggc gttttttccc ttattcccgc tgtgccttta caacttatga 3660

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 3720

3661 gtatgagaag gaaaaagtta taataacttc gtaaatagtc ccaataacag agtactcgcc 3720

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

3721 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 3780

3721 tatgtataaa cttacataaa tctttttatt tgtttatccc caaggcgcgt gtaaaggggc 3780

AmpR_pro .......... .......... .......... .......... ..........

......... AatII(3798) ......... .......... .......... ..........

3781 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 3840

3781 ttttcacggt ggactgcaga ttctttggta ataatagtac tgtaattgga tatttttatc 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcgtatcacg aggccctttc gtcttcactc gacgctggtc gagctgatac ttcccgtccg 3900

3841 cgcatagtgc tccgggaaag cagaagtgag ctgcgaccag ctcgactatg aagggcaggc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccaggggaca tgccggcgat gctgaaggtc gcgcgcattc ccgatgaaga ggccggttac 3960

3901 ggtcccctgt acggccgcta cgacttccag cgcgcgtaag ggctacttct ccggccaatg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... NarI(3989) .......... .......... ..........

3961 cgcctgttga cctggtggga cgggcagggc gccgcccgag tcttgcctcg gcggcgggcg 4020

3961 gcggacaact ggaccaccct gcccgtcccg cggcgggctc agaacggagc cgccgcccgc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctctgctcat ggagcgcgcg tccggggacc ttgcacagat agcgtggtcc ggccagacga 4080

4021 gagacgagta cctcgcgcgc aggcccctgg aacgtgtcta tcgcaccagg ccggtctgct 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgaggcttgc aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 4140

4081 gctccgaacg tcctagtatt agtcggtatg gtgtaaacat ctccaaaatg aacgaaattt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 4200

4141 tttggagggt gtggaggggg acttggactt tgtattttac ttacgttaac aacaacaatt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 4260

4201 gaacaaataa cgtcgaatat taccaatgtt tatttcgtta tcgtagtgtt taaagtgttt 4260

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

4261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 4320

4261 atttcgtaaa aaaagtgacg taagatcaac accaaacagg tttgagtagt tacatagaat 4320

.......... ........ SV40_PA_term EBV_rev_pri ........ ..........

.......... ........ SalI(4346) HindIII(4352) ........ ..........

4321 tcatgtctgg atcctctaga actaggtcga caagcttgat atcgaattct tacttgtaca 4380

4321 agtacagacc taggagatct tgatccagct gttcgaacta tagcttaaga atgaacatgt 4380

SV40_PA_term ........ .......... .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

4381 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat 4440

4381 cgagcaggta cggctctcac tagggccgcc gccagtgctt gaggtcgtcc tggtacacta 4440

.......... .......... ORF 2 EGFP .......... EGFP_C_pri ..........

.......... .......... .......... .......... .......... ..........

4441 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg 4500

4441 gcgcgaagag caaccccaga aacgagtccc gcctgaccca cgagtccatc accaacagcc 4500

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca 4560

4501 cgtcgtcgtg ccccggcagc ggctaccccc acaagacgac catcaccagc cgctcgacgt 4560

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct 4620

4561 gcgacggcag gagctacaac accgcctaga acttcaagtg gaactacggc aagaagacga 4620

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga 4680

4621 acagccggta ctatatctgc aacaccgaca acatcaacat gaggtcgaac acggggtcct 4680

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc 4740

4681 acaacggcag gaggaacttc agctacggga agtcgagcta cgccaagtgg tcccacagcg 4740

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc 4800

4741 ggagcttgaa gtggagccgc gcccagaaca tcaacggcag caggaacttc ttctaccacg 4800

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt 4860

4801 cgaggacctg catcggaagc ccgtaccgcc tgaacttctt cagcacgacg aagtacacca 4860

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg 4920

4861 gccccatcgc cgacttcgtg acgtgcggca tccagtccca ccagtgctcc cacccggtcc 4920

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat 4980

4921 cgtgcccgtc gaacggccac cacgtctact tgaagtccca gtcgaacggc atccaccgta 4980

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga 5040

4981 gcgggagcgg gagcggcctg tgcgacttga acaccggcaa atgcagcggc aggtcgagct 5040

.......... .......... ORF 2 EGFP .......... .......... EGFP_N_pri

.......... .......... .......... .......... .......... ..........

5041 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatc cgcggggatc 5100

5041 ggtcctaccc gtggtggggc cacttgtcga ggagcgggaa cgagtggtag gcgcccctag 5100

.......... .......... ORF 2 EGFP .......... .......... ..........

SpeI(5102) NotI(5115),EagI(5115) ........ .......... ..........

5101 cactagttct agagcggccg c......... .......... .......... .......... 5121

5101 gtgatcaaga tctcgccggc g......... .......... .......... .......... 5121

pBluescriptSK_pri .. .......... .......... .......... ..........