4LOCUS pBI-G 7727 bp DNA SYN 15-Sep-2007 DEFINITION pBI-G ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7727 /organism="pBI-G" /mol_type="other DNA" promoter complement(23..118) /label="CMV2_promoter" misc_feature complement(85..109) /label="LNCX_primer" misc_feature complement(92..111) /label="pCEP_fwd_primer" misc_feature 134..439 /label="TRE" misc_feature 471..490 /label="pCEP_fwd_primer" misc_feature 473..497 /label="LNCX_primer" promoter 440..559 /label="CMV2_promoter" misc_feature complement(694..716) /label="M13_pUC_fwd_primer" CDS 647..3739 /label="lacZ" /gene="lacZ" /note="ORF frame 2" /translation="MGPKKKRKVGSMGDPVVLQRRDWENPGVTQLNRLAAHPPFASWR NSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGY DAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLW CNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRD VSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVAS GTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEA CDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFN AVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNLLTDDPRWLPAMSERVTRM VQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICP MYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQY PRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNHRQFCMNGLVFADRTPHPAL TEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQ GKQLIELPELPQPESAGQLWLTVRVVQPNATGWSEAGHISAWQQWRLAENLSVTLPAA SHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQLWIGDKKQLLTPLRDQFTRAPLDN DIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTL FISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYP DRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQ QLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK *" gene 692..3736 /label="lacZ" /gene="lacZ" misc_feature 3789..3990 /label="lacY" misc_feature 4027..4599 /label="bGlob_int" terminator 4651..5164 /label="rb_glob_PA_terminator" rep_origin complement(5398..6017) /label="pBR322_origin" CDS complement(6172..7032) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(6172..7032) /label="Ampicillin" /gene="Ampicillin" promoter complement(7074..7102) /label="AmpR_promoter" terminator 7571..7702 /label="SV40_PA_terminator" misc_feature 7659..7678 /label="EBV_rev_primer" ORIGIN 1 CTGCAGGAAT TCGGGGCCGC GGAGGCTGGA TCGGTCCCGG TGTCTTCTAT GGAGGTCAAA 61 ACAGCGTGGA TGGCGTCTCC AGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA 121 GGTCGAGTTT ACCACTCCCT ATCAGTGATA GAGAAAAGTG AAAGTCGAGT TTACCACTCC 181 CTATCAGTGA TAGAGAAAAG TGAAAGTCGA GTTTACCACT CCCTATCAGT GATAGAGAAA 241 AGTGAAAGTC GAGTTTACCA CTCCCTATCA GTGATAGAGA AAAGTGAAAG TCGAGTTTAC 301 CACTCCCTAT CAGTGATAGA GAAAAGTGAA AGTCGAGTTT ACCACTCCCT ATCAGTGATA 361 GAGAAAAGTG AAAGTCGAGT TTACCACTCC CTATCAGTGA TAGAGAAAAG TGAAAGTCGA 421 GCTCGGTACC CGGGTCGAGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT 481 TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC 541 ACCGGGACCG ATCCAGCCTC CGCGGCCCCG AATTCGAGCT CGGTACCCGG GGATCCTCTA 601 GAGTCGATCG ACGGATCCGG GGAATTCCCC AGTCTCAGGA TCCACCATGG GGCCCAAGAA 661 GAAACGCAAA GTGGGGAGCA TGGGGGATCC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 721 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 781 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG 841 GCGCTTTGCC TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT 901 TCCTGAGGCC GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC 961 CATCTACACC AACGTAACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA 1021 TCCGACGGGT TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA 1081 GACGCGAATT ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG 1141 GGTCGGTTAC GGCCAGGACA GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG 1201 CGCCGGAGAA AACCGCCTCG CGGTGATGGT GCTGCGTTGG AGTGACGGCA GTTATCTGGA 1261 AGATCAGGAT ATGTGGCGGA TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC 1321 GACTACACAA ATCAGCGATT TCCATGTTGC CACTCGCTTT AATGATGATT TCAGCCGCGC 1381 TGTACTGGAG GCTGAAGTTC AGATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT 1441 TTCTTTATGG CAGGGTGAAA CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT 1501 TATCGATGAG CGTGGTGGTT ATGCCGATCG CGTCACACTA CGTCTGAACG TCGAAAACCC 1561 GAAACTGTGG AGCGCCGAAA TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC 1621 CGACGGCACG CTGATTGAAG CAGAAGCCTG CGATGTCGGT TTCCGCGAGG TGCGGATTGA 1681 AAATGGTCTG CTGCTGCTGA ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA 1741 GCATCATCCT CTGCATGGTC AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATATCCTGCT 1801 GATGAAGCAG AACAACTTTA ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG 1861 GTACACGCTG TGCGACCGCT ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA 1921 CGGCATGGTG CCAATGAATC TGCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA 1981 ACGCGTAACG CGAATGGTGC AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT 2041 GGGGAATGAA TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT 2101 CGATCCTTCC CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT 2161 TATTTGCCCG ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG 2221 GTCCATCAAA AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATCC TTTGCGAATA 2281 CGCCCACGCG ATGGGTAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA 2341 GTATCCCCGT TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA 2401 TGATGAAAAC GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACCA 2461 TCGCCAGTTC TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCGCTGAC 2521 GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA TCCGGGCAAA CCATCGAAGT 2581 GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC CTGCACTGGA TGGTGGCGCT 2641 GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT GTCGCTCCAC AAGGTAAACA 2701 GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC GGGCAACTCT GGCTCACAGT 2761 ACGCGTAGTG CAACCGAACG CGACCGGATG GTCAGAAGCC GGGCACATCA GCGCCTGGCA 2821 GCAGTGGCGT CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC 2881 GCATCTGACC ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT 2941 TAACCGCCAG TCAGGCTTTC TTTCACAGCT GTGGATTGGC GATAAAAAAC AACTGCTGAC 3001 GCCGCTGCGC GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC 3061 GACCCGCATT GACCCTAACG CCTGGGTCGA ACGCTGGAAG GCGGCGGGCC ATTACCAGGC 3121 CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT GATGCGGTGC TGATTACGAC 3181 CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC AGCCGGAAAA CCTACCGGAT 3241 TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA GTGGCGAGCG ATACACCGCA 3301 TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA GCAGAGCGGG TAAACTGGCT 3361 CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT GCCGCCTGTT TTGACCGCTG 3421 GGATCTGCCA TTGTCAGACA TGTATACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG 3481 CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA 3541 CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC 3601 GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC 3661 CTGGAGCCCG TCAGTATCGG CGGAATTACA GCTGAGCGCC GGTCGCTACC ATTACCAGTT 3721 GGTCTGGTGT CAAAAATAAT AATAACCGGC AGGCCATGTC TGAAAGTATT CGCGTAAGGA 3781 AATCCATTAT GTACTATTTA AAAAACACAA ACTTTTGGAT GTTCGGTTTA TTCTTTTTCT 3841 TTTACTTTTT TATCATGGGA GCCTACTTCC CGTTTTTCCC GATTTGGCTA CATGACATCA 3901 ACCATATGAG CAAAAGTGAT ACGGGTATTA TTTTTGCCGC TATTTCTCTG TTGTCGCTAT 3961 TATTCCAACC GCTGTTGGTC TGCTTTCTGA CAAACTCGGC CTCGACTCTA GACTGAGAAC 4021 TTCAGGGTGA GTTTGGGGAC CCTTGATTGT TCTTTCTTTT TCGCTATTGA AAAATTCATG 4081 TTATATGGAG GGGGCAAAGT TTTCAGGGTG TTGTTTAGAA TGGGAAGATG TCCCTTGTAT 4141 CACCATGGAC CCTCATGATA ATTTTGTTTC TTTCACTTTC TACTCTGTTG ACAACCATTG 4201 TCTCCTCTTA TTTTCTTTTC ATTTTCTGTA ACTTTTTTCG TTAAACTTTA GCTTGCATTT 4261 GTAACGAATT TTTAAATTCA CTTTCGTTTA TTTGTCAGAT TGTAAGTACT TTCTCTAATC 4321 ACTTTTTTTT CAAGGCAATC AGGGTAATTA TATTGTACTT CAGCACAGTT TTAGAGAACA 4381 ATTGTTATAA TTAAATGATA AGGTAGAATA TTTCTGCATA TAAATTCTGG CTGGCGTGGA 4441 AATATTCTTA TTGGTAGAAA CAACTACATC CTGGTAATCA TCCTGCCTTT CTCTTTATGG 4501 TTACAATGAT ATACACTGTT TGAGATGAGG ATAAAATACT CTGAGTCCAA ACCGGGCCCC 4561 TCTGCTAACC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA CGTGCTGGTT 4621 GTTGTGCTGT CTCATCATTT TGGCAAAGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG 4681 AAGGTGGTGG CTGGTGTGGC CAATGCCCTG GCTCACAAAT ACCACTGAGA TCTTTTTCCC 4741 TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGGTAATAA 4801 AGGAAATTTA TTTTCATTGC AATAGTGTGT GGGAATTTTT TGTGTCTCTC ACTCGGAAGG 4861 ACATATGGGA GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA 4921 ACATATGCCA TATGCTGGCT GCCATGAACA AAGGTGGCTA TAAAGAGGTC ATCAGTATAT 4981 GAAACAGCCC CCTGCTGTCC ATTCCTTATT CCATAGAAAA GCCTTGACTT GAGGTTAGAT 5041 TTTTTTTATA TTTTGTTTTG TGTTATTTTT TTCTTTAACA TCCCTAAAAT TTTCCTTACA 5101 TGTTTTACTA GCCAGATTTT TCCTCCTCTC CTGACTACTC CCAGTCATAG CTGTCCCTCT 5161 TCTCTTATGA ACTCGACTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 5221 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 5281 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 5341 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 5401 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 5461 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 5521 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 5581 CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 5641 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 5701 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 5761 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 5821 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 5881 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 5941 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 6001 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 6061 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 6121 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 6181 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 6241 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 6301 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 6361 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 6421 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 6481 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 6541 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 6601 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 6661 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 6721 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 6781 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 6841 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 6901 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 6961 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 7021 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 7081 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 7141 ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA 7201 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACTCG ACGCTGGTCG AGCTGATACT 7261 TCCCGTCCGC CAGGGGACAT GCCGGCGATG CTGAAGGTCG CGCGCATTCC CGATGAAGAG 7321 GCCGGTTACC GCCTGTTGAC CTGGTGGGAC GGGCAGGGCG CCGCCCGAGT CTTGCCTCGG 7381 CGGCGGGCGC TCTGCTCATG GAGCGCGCGT CCGGGGACCT TGCACAGATA GCGTGGTCCG 7441 GCCAGACGAC GAGGCTTGCA GGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT 7501 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT 7561 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 7621 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 7681 TGTATCTTAT CATGTCTGGA TCCTCTAGAA CTAGGTCGAC GCGGCCG //+pBI-G "Times New RomanC+vCMV2_pro"Times New RomanC+UmLNCX_pri"Times New RomanCɆ+\o pCEP_fwd_pri"Times New Roman*+TRE"Times New RomanCɆ+/CMV2_pro"Times New Roman(C+ pCEP_fwd_pri"Times New Roman=C+LNCX_pri"Times New RomanC+ORF 2"Times New Roman(C+lacZ"Times New Roman+M13_pUC_fwd_pri"Times New RomanCɆ+lacY"Times New Roman+ bGlob_int"Times New RomanC++,rb_glob_PA_term"Times New RomanC+ pBR322_origin"Times New RomanCɆ+xORF 1"Times New Roman+x Ampicillin"Times New RomanC+AmpR_pro"Times New RomanC+ SV40_PA_term"Times New RomanCɆ+ EBV_rev_pri"Times New Roman PstI"Times New Roman+ StuI"Times New Roman+ R ClaI"Times New Roman+xqjcq EcoRV"Times New Roman+ BclI"Times New Roman+ MscI(4699),BglII(4728)"Times New Roman[+"y NarI"Times New Roman+ke^@|  SalI(7715),NotI(7722),EagI(7722)"Times New Roman#+R test"Times New Roman+7