Sequence of pBI-G

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Sequence of pBI-G

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Sequence of pBI-G : 7727 BP; 1853 A; 1934 C; 1954 G; 1986 T; 0 other;

PstI(5) .......... .......... .......... .......... ..........

1 ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa 60

1 gacgtcctta agccccggcg cctccgacct agccagggcc acagaagata cctccagttt 60

.......... .......... .......... .......... CMV2_pro ..........

.......... .......... .......... .......... .......... ..........

61 acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata 120

61 tgtcgcacct accgcagagg tccgctagac tgccaagtga tttgctcgag acgaatatat 120

.......... ......... CMV2_pro LNCX_pri pCEP_fwd_pri .........

.......... .......... .......... .......... .......... ..........

121 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc 180

121 ccagctcaaa tggtgaggga tagtcactat ctcttttcac tttcagctca aatggtgagg 180

.......... .......... .......... TRE .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa 240

181 gatagtcact atctcttttc actttcagct caaatggtga gggatagtca ctatctcttt 240

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac 300

241 tcactttcag ctcaaatggt gagggatagt cactatctct tttcactttc agctcaaatg 300

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata 360

301 gtgagggata gtcactatct cttttcactt tcagctcaaa tggtgaggga tagtcactat 360

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 420

361 ctcttttcac tttcagctca aatggtgagg gatagtcact atctcttttc actttcagct 420

.......... .......... TRE .......... .......... ..........

.......... .......... .......... StuI(459) .......... ..........

421 gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt 480

421 cgagccatgg gcccagctca tccgcacatg ccaccctccg gatatattcg tctcgagcaa 480

TRE .......... .......... .......... .... CMV2_pro pCEP_fwd_pri

.......... .......... .......... .......... .......... ..........

481 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac 540

481 atcacttggc agtctagcgg acctctgcgg taggtgcgac aaaactggag gtatcttctg 540

pCEP_fwd_pri LNCX_pri CMV2_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta 600

541 tggccctggc taggtcggag gcgccggggc ttaagctcga gccatgggcc cctaggagat 600

CMV2_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gagtcgatcg acggatccgg ggaattcccc agtctcagga tccaccatgg ggcccaagaa 660

601 ctcagctagc tgcctaggcc ccttaagggg tcagagtcct aggtggtacc ccgggttctt 660

.......... .......... .......... .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

661 gaaacgcaaa gtggggagca tgggggatcc cgtcgtttta caacgtcgtg ac tgggaaaa 720

661 ctttgcgttt cacccctcgt accccctagg gcagcaaaat gttgcagcac tgaccctttt 720

.......... .......... ORF 2 ..... lacZ M13_pUC_fwd_pri .....

.......... .......... .......... .......... .......... ..........

721 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 780

721 gggaccgcaa tgggttgaat tagcggaacg tcgtgtaggg ggaaagcggt cgaccgcatt 780

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 840

781 atcgcttctc cgggcgtggc tagcgggaag ggttgtcaac gcgtcggact taccgcttac 840

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct 900

841 cgcgaaacgg accaaaggcc gtggtcttcg ccacggcctt tcgaccgacc tcacgctaga 900

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc 960

901 aggactccgg ctatgacagc agcaggggag tttgaccgtc tacgtgccaa tgctacgcgg 960

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa 1020

961 gtagatgtgg ttgcattgga tagggtaatg ccagttaggc ggcaaacaag ggtgcctctt 1020

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca 1080

1021 aggctgccca acaatgagcg agtgtaaatt acaactactt tcgaccgatg tccttccggt 1080

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg 1140

1081 ctgcgcttaa taaaaactac cgcaattgag ccgcaaagta gacaccacgt tgcccgcgac 1140

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg 1200

1141 ccagccaatg ccggtcctgt cagcaaacgg cagacttaaa ctggactcgc gtaaaaatgc 1200

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg agtgacggca gttatctgga 1260

1201 gcggcctctt ttggcggagc gccactacca cgacgcaacc tcactgccgt caatagacct 1260

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc 1320

1261 tctagtccta tacaccgcct actcgccgta aaaggcactg cagagcaacg acgtatttgg 1320

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc 1380

1321 ctgatgtgtt tagtcgctaa aggtacaacg gtgagcgaaa ttactactaa agtcggcgcg 1380

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt 1440

1381 acatgacctc cgacttcaag tctacacgcc gctcaacgca ctgatggatg cccattgtca 1440

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat 1500

1441 aagaaatacc gtcccacttt gcgtccagcg gtcgccgtgg cgcggaaagc cgccacttta 1500

.......... .......... ORF 2 lacZ .......... .......... ..........

ClaI(1503) .......... .......... .......... .......... ..........

1501 tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc 1560

1501 atagctactc gcaccaccaa tacggctagc gcagtgtgat gcagacttgc agcttttggg 1560

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc 1620

1561 ctttgacacc tcgcggcttt agggcttaga gatagcacgc caccaacttg acgtgtggcg 1620

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga 1680

1621 gctgccgtgc gactaacttc gtcttcggac gctacagcca aaggcgctcc acgcctaact 1680

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga 1740

1681 tttaccagac gacgacgact tgccgttcgg caacgactaa gctccgcaat tggcagtgct 1740

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(1792)

1741 gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct 1800

1741 cgtagtagga gacgtaccag tccagtacct actcgtctgc taccacgtcc tataggacga 1800

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg 1860

1801 ctacttcgtc ttgttgaaat tgcggcacgc gacaagcgta ataggcttgg taggcgacac 1860

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca 1920

1861 catgtgcgac acgctggcga tgccggacat acaccaccta cttcggttat aactttgggt 1920

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cggcatggtg ccaatgaatc tgctgaccga tgatccgcgc tggctaccgg cgatgagcga 1980

1921 gccgtaccac ggttacttag acgactggct actaggcgcg accgatggcc gctactcgct 1980

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... BclI(2025) ..........

1981 acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct 2040

1981 tgcgcattgc gcttaccacg tcgcgctagc attagtgggc tcacactagt agaccagcga 2040

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt 2100

2041 ccccttactt agtccggtgc cgcgattagt gctgcgcgac atagcgacct agtttagaca 2100

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat 2160

2101 gctaggaagg gcgggccacg tcatacttcc gccgcctcgg ctgtggtgcc ggtggctata 2160

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg 2220

2161 ataaacgggc tacatgcgcg cgcacctact tctggtcggg aagggccgac acggctttac 2220

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata 2280

2221 caggtagttt tttaccgaaa gcgatggacc tctctgcgcg ggcgactagg aaacgcttat 2280

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca 2340

2281 gcgggtgcgc tacccattgt cagaaccgcc aaagcgattt atgaccgtcc gcaaagcagt 2340

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata 2400

2341 cataggggca aatgtcccgc cgaagcagac cctgacccac ctagtcagcg actaatttat 2400

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacca 2460

2401 actacttttg ccgttgggca ccagccgaat gccgccacta aaaccgctat gcggcttggt 2460

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac 2520

2461 agcggtcaag acatacttgc cagaccagaa acggctggcg tgcggcgtag gtcgcgactg 2520

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt 2580

2521 ccttcgtttt gtggtcgtcg tcaaaaaggt caaggcaaat aggcccgttt ggtagcttca 2580

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct 2640

2581 ctggtcgctt atggacaagg cagtatcgct attgctcgag gacgtgacct accaccgcga 2640

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca 2700

2641 cctaccattc ggcgaccgtt cgccacttca cggagaccta cagcgaggtg ttccatttgt 2700

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt 2760

2701 caactaactt gacggacttg atggcgtcgg cctctcgcgg cccgttgaga ccgagtgtca 2760

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acgcgtagtg caaccgaacg cgaccggatg gtcagaagcc gggcacatca gcgcctggca 2820

2761 tgcgcatcac gttggcttgc gctggcctac cagtcttcgg cccgtgtagt cgcggaccgt 2820

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc 2880

2821 cgtcaccgca gaccgccttt tggagtcaca ctgcgagggg cggcgcaggg tgcggtaggg 2880

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt 2940

2881 cgtagactgg tggtcgcttt acctaaaaac gtagctcgac ccattattcg caaccgttaa 2940

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taaccgccag tcaggctttc tttcacagct gtggattggc gataaaaaac aactgctgac 3000

2941 attggcggtc agtccgaaag aaagtgtcga cacctaaccg ctattttttg ttgacgactg 3000

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc 3060

3001 cggcgacgcg ctagtcaagt gggcacgtgg cgacctattg ctgtaaccgc attcacttcg 3060

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc 3120

3061 ctgggcgtaa ctgggattgc ggacccagct tgcgaccttc cgccgcccgg taatggtccg 3120

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac 3180

3121 gcttcgtcgc aacaacgtca cgtgccgtct atgtgaacga ctacgccacg actaatgctg 3180

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat 3240

3181 gcgagtgcgc accgtcgtag tccccttttg gaataaatag tcggcctttt ggatggccta 3240

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca 3300

3241 actaccatca ccagtttacc gctaatggca actacaactt caccgctcgc tatgtggcgt 3300

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct 3360

3301 aggccgcgcc taaccggact tgacggtcga ccgcgtccat cgtctcgccc atttgaccga 3360

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg 3420

3361 gcctaatccc ggcgttcttt tgatagggct ggcggaatga cggcggacaa aactggcgac 3420

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg 3480

3421 cctagacggt aacagtctgt acatatgggg catgcagaag ggctcgcttt tgccagacgc 3480

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa 3540

3481 gacgccctgc gcgcttaact taataccggg tgtggtcacc gcgccgctga aggtcaagtt 3540

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc 3600

3541 gtagtcggcg atgtcagttg tcgttgacta cctttggtcg gtagcggtag acgacgtgcg 3600

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc 3660

3601 ccttcttccg tgtaccgact tatagctgcc aaaggtatac ccctaaccac cgctgctgag 3660

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctggagcccg tcagtatcgg cggaattaca gctgagcgcc ggtcgctacc attaccagtt 3720

3661 gacctcgggc agtcatagcc gccttaatgt cgactcgcgg ccagcgatgg taatggtcaa 3720

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtctggtgt caaaaataat aataaccggc aggccatgtc tgaaagtatt cgcgtaagga 3780

3721 ccagaccaca gtttttatta ttattggccg tccggtacag actttcataa gcgcattcct 3780

ORF 2 lacZ .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aatccattat gtactattta aaaaacacaa acttttggat gttcggttta ttctttttct 3840

3781 ttaggtaata catgataaat tttttgtgtt tgaaaaccta caagccaaat aagaaaaaga 3840

.......... .......... .......... lacY .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tttacttttt tatcatggga gcctacttcc cgtttttccc gatttggcta catgacatca 3900

3841 aaatgaaaaa atagtaccct cggatgaagg gcaaaaaggg ctaaaccgat gtactgtagt 3900

.......... .......... lacY .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 accatatgag caaaagtgat acgggtatta tttttgccgc tatttctctg ttgtcgctat 3960

3901 tggtatactc gttttcacta tgcccataat aaaaacggcg ataaagagac aacagcgata 3960

.......... .......... lacY .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tattccaacc gctgttggtc tgctttctga caaactcggc ctcgactcta gactgagaac 4020

3961 ataaggttgg cgacaaccag acgaaagact gtttgagccg gagctgagat ctgactcttg 4020

.......... lacY .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattga aaaattcatg 4080

4021 aagtcccact caaacccctg ggaactaaca agaaagaaaa agcgataact ttttaagtac 4080

.......... .......... .......... bGlob_int .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat 4140

4081 aatatacctc ccccgtttca aaagtcccac aacaaatctt acccttctac agggaacata 4140

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 caccatggac cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg 4200

4141 gtggtacctg ggagtactat taaaacaaag aaagtgaaag atgagacaac tgttggtaac 4200

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tctcctctta ttttcttttc attttctgta acttttttcg ttaaacttta gcttgcattt 4260

4201 agaggagaat aaaagaaaag taaaagacat tgaaaaaagc aatttgaaat cgaacgtaaa 4260

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtaacgaatt tttaaattca ctttcgttta tttgtcagat tgtaagtact ttctctaatc 4320

4261 cattgcttaa aaatttaagt gaaagcaaat aaacagtcta acattcatga aagagattag 4320

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actttttttt caaggcaatc agggtaatta tattgtactt cagcacagtt ttagagaaca 4380

4321 tgaaaaaaaa gttccgttag tcccattaat ataacatgaa gtcgtgtcaa aatctcttgt 4380

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga 4440

4381 taacaatatt aatttactat tccatcttat aaagacgtat atttaagacc gaccgcacct 4440

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aatattctta ttggtagaaa caactacatc ctggtaatca tcctgccttt ctctttatgg 4500

4441 ttataagaat aaccatcttt gttgatgtag gaccattagt aggacggaaa gagaaatacc 4500

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc 4560

4501 aatgttacta tatgtgacaa actctactcc tattttatga gactcaggtt tggcccgggg 4560

.......... .......... bGlob_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt 4620

4561 agacgattgg tacaagtacg gaagaagaaa aaggatgtcg aggacccgtt gcacgaccaa 4620

.......... bGlob_int .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc aggtgcaggc tgcctatcag 4680

4621 caacacgaca gagtagtaaa accgtttctt aagtgaggag tccacgtccg acggatagtc 4680

.......... .......... .......... ....... rb_glob_PA_term .......

.......... MscI(4699) .......... ......... BglII(4728) .........

4681 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc 4740

4681 ttccaccacc gaccacaccg gttacgggac cgagtgttta tggtgactct agaaaaaggg 4740

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tgggtaataa 4800

4741 agacggtttt taatacccct gtagtacttc ggggaactcg tagactgaag acccattatt 4800

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aggaaattta ttttcattgc aatagtgtgt gggaattttt tgtgtctctc actcggaagg 4860

4801 tcctttaaat aaaagtaacg ttatcacaca cccttaaaaa acacagagag tgagccttcc 4860

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca 4920

4861 tgtataccct cccgtttagt aaattttgta gtcttactca taaaccaaat ctcaaaccgt 4920

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc atcagtatat 4980

4921 tgtatacggt atacgaccga cggtacttgt ttccaccgat atttctccag tagtcatata 4980

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat 5040

4981 ctttgtcggg ggacgacagg taaggaataa ggtatctttt cggaactgaa ctccaatcta 5040

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca 5100

5041 aaaaaaatat aaaacaaaac acaataaaaa aagaaattgt agggatttta aaaggaatgt 5100

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct 5160

5101 acaaaatgat cggtctaaaa aggaggagag gactgatgag ggtcagtatc gacagggaga 5160

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tctcttatga actcgactgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 5220

5161 agagaatact tgagctgacg taattactta gccggttgcg cgcccctctc cgccaaacgc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 5280

5221 ataacccgcg agaaggcgaa ggagcgagtg actgagcgac gcgagccagc aagccgacgc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 5340

5281 cgctcgccat agtcgagtga gtttccgcca ttatgccaat aggtgtctta gtcccctatt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 5400

5341 gcgtcctttc ttgtacactc gttttccggt cgttttccgg tccttggcat ttttccggcg 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5460

5401 caacgaccgc aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt tagctgcgag 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5520

5461 ttcagtctcc accgctttgg gctgtcctga tatttctatg gtccgcaaag ggggaccttc 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5580

5521 gagggagcac gcgagaggac aaggctggga cggcgaatgg cctatggaca ggcggaaaga 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta 5640

5581 gggaagccct tcgcaccgcg aaagagttac gagtgcgaca tccatagagt caagccacat 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 5700

5641 ccagcaagcg aggttcgacc cgacacacgt gcttgggggg caagtcgggc tggcgacgcg 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5760

5701 gaataggcca ttgatagcag aactcaggtt gggccattct gtgctgaata gcggtgaccg 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5820

5761 tcgtcggtga ccattgtcct aatcgtctcg ctccatacat ccgccacgat gtctcaagaa 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 5880

5821 cttcaccacc ggattgatgc cgatgtgatc ttcctgtcat aaaccataga cgcgagacga 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 5940

5881 cttcggtcaa tggaagcctt tttctcaacc atcgagaact aggccgtttg tttggtggcg 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 6000

5941 accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 6060

6001 tcttctagga aactagaaaa gatgccccag actgcgagtc accttgcttt tgagtgcaat 6060

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 6120

6061 tccctaaaac cagtactcta atagtttttc ctagaagtgg atctaggaaa atttaatttt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 6180

6121 tacttcaaaa tttagttaga tttcatatat actcatttga accagactgt caatggttac 6180

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

6181 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 6240

6181 gaattagtca ctccgtggat agagtcgcta gacagataaa gcaagtaggt atcaacggac 6240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 6300

6241 tgaggggcag cacatctatt gatgctatgc cctcccgaat ggtagaccgg ggtcacgacg 6300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 6360

6301 ttactatggc gctctgggtg cgagtggccg aggtctaaat agtcgttatt tggtcggtcg 6360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 6420

6361 gccttcccgg ctcgcgtctt caccaggacg ttgaaatagg cggaggtagg tcagataatt 6420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 6480

6421 aacaacggcc cttcgatctc attcatcaag cggtcaatta tcaaacgcgt tgcaacaacg 6480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 6540

6481 gtaacgatgt ccgtagcacc acagtgcgag cagcaaacca taccgaagta agtcgaggcc 6540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 6600

6541 aagggttgct agttccgctc aatgtactag ggggtacaac acgttttttc gccaatcgag 6600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 6660

6601 gaagccagga ggctagcaac agtcttcatt caaccggcgt cacaatagtg agtaccaata 6660

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 6720

6661 ccgtcgtgac gtattaagag aatgacagta cggtaggcat tctacgaaaa gacactgacc 6720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 6780

6721 actcatgagt tggttcagta agactcttat cacatacgcc gctggctcaa cgagaacggg 6780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 6840

6781 ccgcagttat gccctattat ggcgcggtgt atcgtcttga aattttcacg agtagtaacc 6840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 6900

6841 ttttgcaaga agccccgctt ttgagagttc ctagaatggc gacaactcta ggtcaagcta 6900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 6960

6901 cattgggtga gcacgtgggt tgactagaag tcgtagaaaa tgaaagtggt cgcaaagacc 6960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 7020

6961 cactcgtttt tgtccttccg ttttacggcg ttttttccct tattcccgct gtgcctttac 7020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 7080

7021 aacttatgag tatgagaagg aaaaagttat aataacttcg taaatagtcc caataacaga 7080

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 7140

7081 gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc aaggcgcgtg 7140

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 7200

7141 taaaggggct tttcacggtg gactgcagat tctttggtaa taatagtact gtaattggat 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 taaaaatagg cgtatcacga ggccctttcg tcttcactcg acgctggtcg agctgatact 7260

7201 atttttatcc gcatagtgct ccgggaaagc agaagtgagc tgcgaccagc tcgactatga 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tcccgtccgc caggggacat gccggcgatg ctgaaggtcg cgcgcattcc cgatgaagag 7320

7261 agggcaggcg gtcccctgta cggccgctac gacttccagc gcgcgtaagg gctacttctc 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NarI(7358) .......... ..........

7321 gccggttacc gcctgttgac ctggtgggac gggcagggcg ccgcccgagt cttgcctcgg 7380

7321 cggccaatgg cggacaactg gaccaccctg cccgtcccgc ggcgggctca gaacggagcc 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 cggcgggcgc tctgctcatg gagcgcgcgt ccggggacct tgcacagata gcgtggtccg 7440

7381 gccgcccgcg agacgagtac ctcgcgcgca ggcccctgga acgtgtctat cgcaccaggc 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 gccagacgac gaggcttgca ggatcataat cagccatacc acatttgtag aggttttact 7500

7441 cggtctgctg ctccgaacgt cctagtatta gtcggtatgg tgtaaacatc tccaaaatga 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 7560

7501 acgaaatttt ttggagggtg tggaggggga cttggacttt gtattttact tacgttaaca 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 7620

7561 acaacaattg aacaaataac gtcgaatatt accaatgttt atttcgttat cgtagtgttt 7620

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

7621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca a actcatcaa 7680

7621 aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt 7680

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .... SalI(7715) NotI(7722),EagI(7722) ....

7681 tgtatcttat catgtctgga tcctctagaa ctaggtcgac gcggccg... .......... 7727

7681 acatagaata gtacagacct aggagatctt gatccagctg cgccggc... .......... 7727

......... SV40_PA_term ......... .......... .......... ..........