])])NID EU886197; SV 1; circular; other DNA; STD; SYN; 13144 BP. AC EU886197; DT 30-SEP-2008 (Rel. 97, Created) DT 30-SEP-2008 (Rel. 97, Last updated, Version 1) DE Transformation vector pBISN1-IN, complete sequence. KW . OS Transformation vector pBISN1-IN OC other sequences; artificial sequences; vectors. RN [1] RP 1-13144 RA Abdeeva I.A., Abdeev R.M., Piruzian E.S.; RT ; RL Submitted (15-JUL-2008) to the EMBL/GenBank/DDBJ databases. RL Laboratory of Functional Genomics, Vavilov's Institute of General Genetics RL Russian Academy of Science, Gubkina, Moscow 119991, Russia FH Key Location/Qualifiers FT source 1..13144 FT /organism="Transformation vector pBISN1-IN" FT /mol_type="other DNA" FT /note="Escherichia coli/Agrobacterium tumefaciens FT transformation vector" FT /db_xref="taxon:553236" FT misc_feature complement(6043..6190) FT /note="T-DNA level border" FT polyA_signal complement(6773..6977) FT gene complement(7000..7659) FT /gene="licBM2" FT CDS complement(7000..7659) FT /codon_start=1 FT /transl_table=11 FT /gene="licBM2" FT /product="lichenase" FT /note="beta-1,3-1,4-glucanase" FT /protein_id="ACI16381.1" FT /translation="MVNTPFVAVFSNFDSSQWEKADWANGSVFNCVWKPSQVTFSNGKM FT ILTLDREYGGSYPYKSGEYRTKSFFGYGYYEVRMKAAKNVGIVSSFFTYTGPSDNNPWD FT EIDIEFLGKDTTKVQFNWYKNGVGGNEYLHNLGFDASQDFHTYGFEWRPDYIDFYVDGK FT KVYRGTRNIPVTPGKIMMNLWPGIGVDEWLGRYDGRTPLQAEYEYVKYYPNGKDEL" FT sig_peptide complement(7003..7014) FT /gene="licBM2" FT /note="signal peptide KDEL tailed" FT misc_feature complement(7662..7732) FT /note="leader signal of the Vicia faba leB4" FT promoter complement(7733..8181) FT /note="35S CaMV promoter" FT terminator complement(8868..9123) FT /note="NOS termination signal" FT gene complement(9513..10307) FT /gene="nptII" FT CDS complement(9513..10307) FT /codon_start=1 FT /transl_table=11 FT /gene="nptII" FT /product="neomycin phosphotransferase" FT /protein_id="ACI16380.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT promoter complement(10320..10626) FT /note="Pnos promoter" FT misc_feature complement(10627..10788) FT /note="T-DNA right border" SQ Sequence 13144 BP; 3127 A; 3449 C; 3646 G; 2922 T; 0 other; ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg 120 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac 180 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc 240 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga 300 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat 360 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat 420 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt 480 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg 540 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc 600 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc 660 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg 720 atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg 780 ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg 840 ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg 900 gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg 960 ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140 agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320 atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620 gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680 gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800 ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920 tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980 ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040 aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060 attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580 ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640 cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700 tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760 tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820 cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180 taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300 aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600 agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattctca 6780 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat 6840 acatactaag ggtttcttat atgctcaaca catgagcgaa ccctataaga accctaattc 6900 ccttatctgg gaactactca cacattattc tggagaaaaa tagagagaga tagatttgta 6960 gagagagact gcggactcta gatagatccg atttctagat catagctcat ctttaccgtt 7020 aggatagtat tttacatatt cgtactccgc ctgcaaagga gttcttccgt cgtaacgtcc 7080 caaccattca tccactccta ttcctggcca caaattcatc ataattttgc cgggagtaac 7140 aggtatgttc ctggttccac gataaacttt tttgccgtca acatagaagt ctatataatc 7200 cggcctccat tcaaatccat atgtatgaaa atcctgggaa gcatcgaatc caagattgtg 7260 caaatactcg tttccaccga ctccattttt gtaccagttg aactgaactt tagttgtgtc 7320 ctttcctaaa aactcgatat cgatttcgtc ccatggattg ttgtccgaag gtcctgtata 7380 agtgaagaaa gatgaaacaa ttcctacgtt tttggcagct ttcattctta cttcataata 7440 accgtatccg aaaaatgatt ttgtacgata ttcaccgctt ttatacggat atgaaccgcc 7500 atattccctg tcaagggtca aaatcatttt accgttcgaa aatgtcacct gtgaaggctt 7560 ccaaacacag ttgaacaccg aaccgttcgc ccaatccgct ttttcccact gactggagtc 7620 aaagttcgaa aacactgcaa caaaaggcgt atttaccata aagcttgcta aacatgtgct 7680 tgtaaagaga agcaaggaaa gtgaaagcaa agatagaaaa ggtttggaca tgtcgacgtc 7740 ctctccaaat gaaatgaact tccttatata gagggaaggg tcttgcgaaa atagtgggat 7800 tgtgcgtcat cccttacgtc agtggaaata tcacatcaat ccacttgctt tgaagacgtg 7860 gttggaacgt cttctttttc cacgatgttc ctcgtgggtg ggggtccatc tttgggacca 7920 ctgtcggtag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag 7980 aagccatctt ccttttctac tgtcctttcg atgaagtgac agatagctgg gcaatggaat 8040 ccgaggaggt ttccgaatat taccctttgt tgaaaagtct caatagccct ttgaaaagtc 8100 tcaatagccc tctggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg 8160 tcgtgctcca ccatgttgac ggatccttct cagcttggcg taatcatggt catagctgtt 8220 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 8280 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt gcgctcactg 8340 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 8400 gggagaggcg gtttgcgtat tgggccaaag acaaaagggc gacattcaac cgattgaggg 8460 agggaaggta aatattgacg gaaattattc attaaaggtg aattatcacc gtcaccgact 8520 tgagccattt gggaattaga gccagcaaaa tcaccagtag caccattacc attagcaagg 8580 ccggaaacgt caccaatgaa accatcgata gcagcaccgt aatcagtagc gacagaatca 8640 agtttgcctt tagcgtcaga ctgtagcgcg ttttcatcgg cattttcggt catagccccc 8700 ttattagcgt ttgccatctt ttcataatca aaatcaccgg aaccagagcc accaccggaa 8760 ccgcctccct cagagccgcc accctcagaa ccgccaccct cagagccacc accctcagag 8820 ccgccaccag aaccaccacc agagccgccg ccagcattga caggaggccc gatctagtaa 8880 catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat 8940 cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt 9000 catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat 9060 catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg 9120 atcggggatc atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat 9180 atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc 9240 gggcccgatc atattgtcgc tcaggatcgt ggcgttgtgc ttgtcggccg ttgctgtcgt 9300 aatgatatcg gcaccttcga ccgcctgttc cgcagagatc ccgtgggcga agaactccag 9360 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc 9420 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc 9480 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag 9540 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc 9600 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg 9660 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg 9720 atattcggca agcaggcatc gccatgggtc acgacgagat catcgccgtc gggcatgcgc 9780 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca 9840 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct 9900 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc 9960 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact 10020 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa 10080 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg 10140 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac 10200 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc 10260 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcca 10320 gatccggtgc agattatttg gattgagagt gaatatgaga ctctaattgg ataccgaggg 10380 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac 10440 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac 10500 tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta 10560 aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc 10620 atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt 10680 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt 10740 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc 10800 tggcgccggc cagcgagacg agcaagattg gccgccgccc gaaacgatcc gacagcgcgc 10860 ccagcacagg tgcgcaggca aattgcacca acgcatacag cgccagcaga atgccatagt 10920 gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa 10980 tcaggccgat gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtatgt 11040 tgggtttcac gtctggcctc cggaccagcc tccgctggtc cgattgaacg cgcggattct 11100 ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt caagcatgac 11160 aaagttgcag ccgaatacag tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt 11220 agacggtctg acgacacgca aactggcgga acggttgggg gttcagcagc cggcgcttta 11280 ctggcacttc aggaacaagc gggcgctgct cgacgcactg gccgaagcca tgctggcgga 11340 gaatcatacg cattcggtgc cgagagccga cgacgactgg cgctcatttc tgatcgggaa 11400 tgcccgcagc ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg 11460 cacgcgaccg ggcgcaccgc agatggaaac ggccgacgcg cagcttcgct tcctctgcga 11520 ggcgggtttt tcggccgggg acgccgtcaa tgcgctgatg acaatcagct acttcactgt 11580 tggggccgtg cttgaggagc aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt 11640 tgaacaggct ccgctctcgc cgctgttgcg ggccgcgata gacgccttcg acgaagccgg 11700 tccggacgca gcgttcgagc agggactcgc ggtgattgtc gatggattgg cgaaaaggag 11760 gctcgttgtc aggaacgttg aaggaccgag aaagggtgac gattgatcag gaccgctgcc 11820 ggagcgcaac ccactcacta cagcagagcc atgtagacaa catcccctcc ccctttccac 11880 cgcgtcagac gcccgtagca gcccgctacg ggctttttca tgccctgccc tagcgtccaa 11940 gcctcacggc cgcgctcggc ctctctggcg gccttctggc gctcttccgc ttcctcgctc 12000 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 12060 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 12120 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 12180 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 12240 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 12300 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gcttttccgc 12360 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc 12420 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg 12480 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt 12540 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac 12600 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg 12660 cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc 12720 ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat 12780 cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg 12840 ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg 12900 tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa 12960 ggtcatgatg ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg 13020 gccggggggt gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact 13080 tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc ctttgcgacg 13140 ctca 13144 X3 pBISN1-IN"Times New Roman ])CEagI(32),NotI(93),EagI(93),EagI(220),EagI(663),SexAI(810),EagI(864) ArialX3^TJ J ])-EcoRV(1168),MluI(1650),PstI(1909),PvuII(2010) ArialrX3dkrra ])!EcoRV(2969),EagI(3578),SfiI(3724) Arial X3_NH6Z ]),NdeI(4282),SexAI(4496),EagI(4714),NcoI(4810) ArialX3 ])CNcoI(5338),EagI(5416),NruI(5561),BglII(5607),EcoRV(5705),BclI(5876) ArialX3-5@-@-u ])9PvuII(6228),PvuII(6685),EcoRI(6773),XbaI(6978),XbaI(6995) ArialuX3(2II  ])ENdeI(7220),EcoRV(7339),ClaI(7341),NcoI(7352),HindIII(7662),SalI(7733) ArialX3oi*JJ ])"BamHI(8182),PvuII(8374),ClaI(8606) Arial X3   ])8EcoRV(9181),ApaI(9246),EagI(9286),EcoRV(9307),NcoI(9743) ArialF$ X3 ])cPvuII(10073),PstI(10130),EagI(10269),NheI(10426),Bsu36I(10442),BclI(10623),PmeI(10653),BglII(10797) Arial(X31 ])/EagI(11491),EagI(11533),BclI(11805),EagI(11948) Arial -X3a ])EagI(12727),EagI(13020) Arial1X3bX}JB2}J])X3ORF 5"Times New Roman])CX34+ORF 4"Times New Roman])CX3 KanR1"Times New Roman])CX3 ORF 1"Times New Roman])X3 :ORF 1"Times New Roman])X3) ORF 4"Times New Roman])X33ORF 2"Times New Roman])CɆX3TjM13_pUC_fwd_primer"Times New Roman])X3bsM13_forward20_primer"Times New Roman])CX3XlicBM2"Times New Roman])X3X3ORF 2"Times New Roman])CX3  M13_reverse_primer"Times New Roman])CX3 4 M13_pUC_rev_primer"Times New Roman])X3B _  lac_promoter"Times New Roman])CɆX3$'ORF 5"Times New Roman])CɆX3)%C(nptII"Times New Roman])CX3y+.ORF 3"Times New Roman])CɆX301oriT_RP4"Times New Roman ])test"Times New Roman<X3/d\'J2p2J