Sequence of pBISN1-IN

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Sequence of pBISN1-IN

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Sequence of pBISN1-IN: 13144 BP; 3127 A; 3449 C; 3646 G; 2922 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60

1 ggcccgacca acgggagcgg cgacccgacc gccggcagat accgggacgt ttgcgcggtc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg 120

61 tttgcggcag cttcggcaca cgctctgtgg cgccggcggc cgcaacacct atggagcgcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac 180

121 ttttgaaccg ggagtgactg tctactcccc gcctgcaact gtgaactccc cggctgagtg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc 240

181 ggccgcgccg caactgtcta ctccccgtcc gagctaaagc cggccgctgc acctcgaccg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga 300

241 gtcggagcgt ttagccgctt ttgcggacta aaatgcgctc aaagggtgtc tactacacct 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat 360

301 gttcggaccc ctattcacgg gacgccataa ctgtgaactc cccgcgctga tgactgtcta 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat 420

361 ctccccgcgc taggaactgt gaactccccg tctcacgact gtctactccc cgcgtggata 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt 480

421 actgtaaact ccccgacagg tgtccgtctt ttaggtcgta aacgttccca aaggcgggca 480

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg 540

481 aaaagccggt ggcgattgga cagaaaattg gacgaaaatt tggttataaa tatttggaac 540

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc 600

541 aaaaattggt cccgacgcgg gacacgcgca ctggcgcgtg cggcttcccc ccacgggggg 600

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc 660

601 gaagagcttg ggagggccgg gcgattgcgc ccggagggta ggggggtccc cgacgcgggg 660

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg 720

661 agccggcgct tgccggagtg gggtttttac cgtcgcgacc gtcaggaacg gtaacggccc 720

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg 780

721 tagccccgtc attgccctac ccgctagtcg ggctcgcgct gcgggccttc gtaactgcac 780

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg 840

781 ggcgtccacg accgtagctg taagtcgctg gtccacggcc cgtcactccc gccgccggac 840

.......... .......... ORF 5 .......... .......... ..........

.......... .......... EagI(864) .......... .......... ..........

841 ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg 900

841 ccaccgccgg acgggaagtg aagccggcag ccccgtaagt gcctgaagta ccgccccggc 900

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg 960

901 cgttaaaaat ggaacccgta agaaccgtat caccagcgcc cacggcacga gcacaagccc 960

.......... ORF 5 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020

961 ccacgctatt tgggtcgctt ggtaaactcc actatccatt ctaatatggc tccatacttt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080

1021 tgctcttaac ctggaaatgt cttaatgaga tacttcgcgg tataaatttt tcgatggttc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140

1081 tgcttctcct acttctccta ctcctccgtc taacggaact tatataactg ttatgactat 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200

1141 tctattatat agaaaatata tcttctatag cggcatacat tcctaaagtc ccccgttccg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260

1201 tatccgtcgc gcgaatagtt atatagatat cttacccgtt tcgtattttt gaacgtacct 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320

1261 gattacgaac tttgggtcct gttattggaa tatcgaacat ttaagatagt attaacccat 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380

1321 tactgaggtt gaataactat cacaaaatac aagtctatta cgggctactg aaacagtacg 1380

.......... .......... .......... ORF 4 .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440

1381 tcgaggtggc taaaactctt gctgtcgctg aaggcagggt cggcacggtc cacgacggag 1440

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500

1441 tctaagtcca atacggcgag ttaagcgacg catatagcga acgactaatg cacgtcgaaa 1500

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560

1501 gggaagtccg ccctaagtat gtcgccggtc ggtaggcagt aggtatagtg gtgcagtttc 1560

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620

1561 ccactgtcgt ccgagtattc tgcggggtcg cagcggtatc acgcaagtgg cttatgcacg 1620

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680

1621 cgttgttggc agaaggcctc tgacagtatg cgcattttgt cggtcgcgac cgcgctaaat 1680

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740

1681 cggggctgta tcggggtgac aagcaggtaa aggcgcgtct gctactgcag tgacgggccg 1740

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800

1741 acatacgcgc tccaatggct gacgccggac tcaaaaaatt cactgcattt tagcacaact 1800

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860

1801 ccggttgcgg gtattacgcc cgacaacggg ccgtaggttg cggtaagtac cggtatagtt 1860

.......... ORF 4 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920

1861 actaaaagac cacgcatggc ccaactcttc gccacattca cttgacgtca acggtacaaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980

1921 atgccgtcac tctcgtctct atcgcgacta caggccgcca cgaaaacggc aatgcgtggt 1980

.......... .......... .......... .......... .......... ..........

.......... ......... PvuII(2010) ......... .......... ..........

1981 ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040

1981 ggggcagtca tcgacttgtc ctccctgtcg actatctgtg tcttcggtga cctcgtggag 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100

2041 tttttgtggt agtatgtgat ttagtcattc aaccgtcgta gtgggtatta acaccaaagt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160

2101 tttagccgag gcagctatga tacaatatgc ggttgaaact tttgttgaaa ctttttcgac 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220

2161 aaaagaccat aaattccaaa atcttacgtt ccttgtcact taacctcaag cagaacaata 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280

2221 ttaatcgaag aaccccatag aaatttatga catcttttct ccttccttta ttatttaccg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340

2281 attttactct tatagtggcc ttaacttttt tgactagctt tttatggcga cgcattttct 2340

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400

2341 atgccttcct tacagaggac gattccatat attcgaccac cctcttttac ttttggatat 2400

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460

2401 aaatttttac tgcctgtcgg ccatatttcc ctggtggata ctacaccttg cccttttcct 2460

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520

2461 gtactacgat accgaccttc ctttcgacgg acaaggtttc caggacgtga aacttgccgt 2520

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580

2521 actaccgacc tcgttagacg agtactcact ccggctaccg caggaaacga gccttctcat 2580

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640

2581 acttctactt gtttcgggac ttttctaata gctcgacata cgcctcacgt agtccgagaa 2640

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700

2641 agtgaggtag ctgtatagcc taacagggat atgcttatcg aatctgtcgg cgaatcggct 2700

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760

2701 taacctaatg aatgacttat tgctagaccg gctacaccta acgcttttga cccttcttct 2760

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820

2761 gtgaggtaaa tttctaggcg cgctcgacat actaaaaaat ttctgccttt tcgggcttct 2820

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880

2821 ccttgaacag aaaagggtgc cgctggaccc tctgtcgttg tagaaacact ttctaccgtt 2880

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940

2881 tcattcaccg aaataactag aaccctcttc gccgtcccgc ctgttcacca tactgtaacg 2940

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000

2941 gaagacgcag gccagctagt ccctcctata gccccttctt gtcatacagc tcgataaaaa 3000

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060

3001 actgaatgac ccctagttcg gactaaccct cttttatttt ataatataaa atgacctact 3060

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120

3061 taacaaaatc atggatctac accgcgttgc tacggccgct gttcgtcctc gcgtggctga 3120

KanR1 ORF 1 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180

3121 agaaggcgta gttcacaaaa ccgagagtcc ggctccgggt gccgttcata aacccgttcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240

3181 ccagcgacca taagcacgtc ccgttctaag ccttatggtt catgctcttc ctgccggtct 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300

3241 gccagatgcc ctggctgaag taacggctat tccacctaat agacctgtgg ttccgtggtc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360

3301 cgcccagttt agtccttatt cccgtgtaac ggggccgcac tcagccccgt tagggcgttc 3360

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420

3361 ctcccactta cttagcctgc aaactggcct tccgtatgtc cgttcttgac tagctgcgcc 3420

.......... .......... ORF 1 ORF 4 .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480

3421 ccaaaaggcg gctcctacgg ctttggtagc gttcggcgtg gcagtacgca cgcggggcgc 3480

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540

3481 tttggaaggt caggcagccg agctaccagg tcgttcgatg ccggttctag ctcgcgctgt 3540

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600

3541 cgcacgttga ccgaggggga cgggacgggc gcggtagccg gcggcacctc gcaagcgcag 3600

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660

3601 cagagcttgt cctccgccgt ccaaaccgct tcagctactg gtagctgtgc gctccttgat 3660

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720

3661 actgctggtt cttcgctttt tggcggccgc tcctggaccg ttttgtccag tcgctccggt 3720

.......... ......... ORF 1 ORF 4 ......... .......... ..........

SfiI(3724) .......... .......... .......... .......... ..........

3721 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780

3721 tcgtccggcg caacgacttt gtgtgcttcg tcgtctagtt cctttacgtc gaaaggaaca 3780

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840

3781 agctataacg cggcaccggc ctgtgctacg ctcgctacgg tttgctgtgc cgggcgagac 3840

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900

3841 gggacaagtg gtgcgcgttg ttcttttagg gcgcgctccg cgacgttttg ttccagtaaa 3900

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960

3901 aggtgcagtt gttcctgcac ttctagtgga tgtggccgca gctcgacgcc cggctgctac 3960

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020

3961 tgcttgacca caccgtcgtc cacaacctca tgcgcttcgc gtggggatag ccgctcggct 4020

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080

4021 agtggaagtg caagatgctc gaaacggtcc tggacccgac cagctagtta ccggccataa 4080

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140

4081 tgtgcttccg gctccttacg gacagcgcgg atgtccgctg ccgctacccg aagtgcaggc 4140

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200

4141 tggcgcaacc cgtggacctt agccacagcg acgacgtggc gaaggcgcag gacctggcac 4200

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260

4201 cgttcttttg cagggcaacg gtccaggact agctgctcct ttagcagcac gacaaacgac 4260

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320

4261 cgctggtgat gtgctttaag tataccctct tcatggcgtt cgacagcggc tgccgggctg 4320

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380

4321 cctacaagct gataaagtcg agcgtggccc tcggcatggg cgagttcgac ctttggaagg 4380

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440

4381 cggagtacac gcctagccta aggtgggcgc acttcttcac cgcgctcgtc cagccgcttc 4440

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500

4441 ggacgcttct caacgctccg tcgccggacc accttgtgcg gacccagtta ctactggacc 4500

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560

4501 acgtaacgtt tgcgatcccg gaacacccca gtcaaggccg acccccaagt cgtcggtcgc 4560

ORF 4 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620

4561 gaaatgaccg taaagtcctt gttcgcccgt gacgagctgc gtgaacgaag cgagtcatag 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680

4621 cgagccctgc gtgccgcgcg agatgcttga cggctatttg tctcctaatt ttaactgtta 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740

4681 acactaattc cgagtctaag ctgccgaacc tcgccggctg cacgtcctaa aggcgctcta 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800

4741 ggctaacagc cgggacttct ttcgaggtct ctacaagccc aggcaaatgc tcgtgctcct 4800

.......... .......... .......... .......... ORF 2 ..........

NcoI(4810) .......... .......... .......... .......... ..........

4801 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860

4801 ctttttcggg tacctccgca agcgacttgc caacgctcta cggcaccgta agccgcggat 4860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920

4861 gtagctgccg ctctagtaac ccgacagcca gaagtttgtc ctcctgccgg ggttcctgcg 4920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980

4921 agtgttccgc gtagacaggc cgcaaaagca cctcgggctt gtcgctccgg ctccccagcg 4980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040

4981 gccatacgac gacgcccgca acggccgccc aaataacgag cactactagc aggctgtcta 5040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100

5041 aggttgccct tagaccacct acgcgtagaa gtaggagccg cgtgaattat aaagcgataa 5100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160

5101 gacctcgaac aacaaataaa gccagatggc ggacggcccg ccccagcgcc gctgccatcc 5160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220

5161 gcgacacgtc ggcgactacc agcacaagta gagacggcga gacgatccat cgggctatgc 5220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280

5221 taactaccgc caggaccccc gataaacgcc ttgacgcccg caccgcgaca accacaactg 5280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340

5281 tggtttgcgt cgcgatctag gacagccgca gcgtcgcccg gaccgccccc gccaaaggta 5340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400

5341 ccgcaagcct tggcacgact gggcgttcac cgttggaggg cacggagacg agtggaaatg 5400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460

5401 gcggaccgtt gaccgccggc ctcctgaaga cgagcaaggt catcgaaatc acaaactagg 5460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520

5461 cggttagggc tacggatgtc cttggttaca agagccggac cgcaccgagc cggactagcc 5520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580

5521 tcgcccaaat tggatgaagg aaaccaaggc cccctagagc gctgagcttg gatgtcaaca 5580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640

5581 aaggaatgac ccgaaagagt cggggtctag accccagcta gtcggcccct acgtagtccg 5640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700

5641 gctgtcagcc ttgaagccca ggggctggac atggtaagcc actcgttacc tatcccctca 5700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760

5701 actatagcag ttgcaagtga agatttcttt atcgcggtga gtcgaaggag tcgccgaaat 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820

5761 aggtcgctaa aggataatac agccgtatca agagttctag ctgtcggaca gtgccaattc 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... BclI(5876)

5821 cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880

5821 gctctttact tattcttccg actattaagc ctagagacgc tccctctact ataaactagt 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940

5881 gtccgtcgtt gcgagacagt agcaatgtta gttgtacgat gggaggcgct ctagtaggca 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000

5941 caaagtttgg gccgtcgaat caacggcaag aaggcttatc gtagccattg tactcgtttc 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060

6001 agacggcgga atgttgccga gagggcgact gcggcagggc ctgactaccc gacggacata 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120

6061 gctcaccact aaaacacggc tcgacggcca gcccctcgac aaccgaccga ccaccgtcct 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180

6121 atataacacc acatttgttt aactgcgaat ctgttgaatt attgtgtaac gcctgcaaaa 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240

6181 attacatgac cccaccaaaa agaaaagtgg tcactctgcc cgttgtcgac taacgggaag 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300

6241 tggcggaccg ggactctctc aacgtcgttc gccaggtgcg accaaacggg gtcgtccgct 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360

6301 tttaggacaa actaccacca aggctttagc cgttttaggg aatatttagt tttcttatcg 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420

6361 ggctctatcc caactcacaa caaggtcaaa ccttgttctc aggtgataat ttcttgcacc 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480

6421 tgaggttgca gtttcccgct ttttggcaga tagtcccgct accgggtgat gcacttggta 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540

6481 gtgggtttag ttcaaaaaac cccagctcca cggcatttcg tgatttagcc ttgggatttc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600

6541 cctcgggggc taaatctcga actgcccctt tcggccgctt gcaccgctct ttccttccct 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660

6601 tctttcgctt tcctcgcccg cggtaagtcc gacgcgttga caacccttcc cgctagccac 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720

6661 gcccggagaa gcgataatgc ggtcgaccgc tttcccccta cacgacgttc cgctaattca 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattctca 6780

6721 acccattgcg gtcccaaaag ggtcagtgct gcaacatttt gctgccggtc acttaagagt 6780

.......... .......... . M13_pUC_fwd_primer M13_forward20_primer .

.......... .......... .......... .......... .......... ..........

6781 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat 6840

6781 gacctaaaac caaaatcctt aatctttaaa ataactatct tcataaaatg tttatgttta 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 acatactaag ggtttcttat atgctcaaca catgagcgaa ccctataaga accctaattc 6900

6841 tgtatgattc ccaaagaata tacgagttgt gtactcgctt gggatattct tgggattaag 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ccttatctgg gaactactca cacattattc tggagaaaaa tagagagaga tagatttgta 6960

6901 ggaatagacc cttgatgagt gtgtaataag acctcttttt atctctctct atctaaacat 6960

.......... .......... .......... .......... .......... ..........

.......... XbaI(6978) .......... XbaI(6995) .......... ..........

6961 gagagagact gcggactcta gatagatccg atttctagat catagctcat ctttaccgtt 7020

6961 ctctctctga cgcctgagat ctatctaggc taaagatcta gtatcgagta gaaatggcaa 7020

.......... .......... .......... ......... licBM2 ORF 2 .........

.......... .......... .......... .......... .......... ..........

7021 aggatagtat tttacatatt cgtactccgc ctgcaaagga gttcttccgt cgtaacgtcc 7080

7021 tcctatcata aaatgtataa gcatgaggcg gacgtttcct caagaaggca gcattgcagg 7080

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 caaccattca tccactccta ttcctggcca caaattcatc ataattttgc cgggagtaac 7140

7081 gttggtaagt aggtgaggat aaggaccggt gtttaagtag tattaaaacg gccctcattg 7140

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 aggtatgttc ctggttccac gataaacttt tttgccgtca acatagaagt ctatataatc 7200

7141 tccatacaag gaccaaggtg ctatttgaaa aaacggcagt tgtatcttca gatatattag 7200

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 cggcctccat tcaaatccat atgtatgaaa atcctgggaa gcatcgaatc caagattgtg 7260

7201 gccggaggta agtttaggta tacatacttt taggaccctt cgtagcttag gttctaacac 7260

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 caaatactcg tttccaccga ctccattttt gtaccagttg aactgaactt tagttgtgtc 7320

7261 gtttatgagc aaaggtggct gaggtaaaaa catggtcaac ttgacttgaa atcaacacag 7320

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .... EcoRV(7339),ClaI(7341) .... .......... ..........

7321 ctttcctaaa aactcgatat cgatttcgtc ccatggattg ttgtccgaag gtcctgtata 7380

7321 gaaaggattt ttgagctata gctaaagcag ggtacctaac aacaggcttc caggacatat 7380

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 agtgaagaaa gatgaaacaa ttcctacgtt tttggcagct ttcattctta cttcataata 7440

7381 tcacttcttt ctactttgtt aaggatgcaa aaaccgtcga aagtaagaat gaagtattat 7440

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 accgtatccg aaaaatgatt ttgtacgata ttcaccgctt ttatacggat atgaaccgcc 7500

7441 tggcataggc tttttactaa aacatgctat aagtggcgaa aatatgccta tacttggcgg 7500

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 atattccctg tcaagggtca aaatcatttt accgttcgaa aatgtcacct gtgaaggctt 7560

7501 tataagggac agttcccagt tttagtaaaa tggcaagctt ttacagtgga cacttccgaa 7560

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ccaaacacag ttgaacaccg aaccgttcgc ccaatccgct ttttcccact gactggagtc 7620

7561 ggtttgtgtc aacttgtggc ttggcaagcg ggttaggcga aaaagggtga ctgacctcag 7620

.......... ......... licBM2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 aaagttcgaa aacactgcaa caaaaggcgt atttaccata aagcttgcta aacatgtgct 7680

7621 tttcaagctt ttgtgacgtt gttttccgca taaatggtat ttcgaacgat ttgtacacga 7680

.......... licBM2 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... SalI(7733)

7681 tgtaaagaga agcaaggaaa gtgaaagcaa agatagaaaa ggtttggaca tgtcgacgtc 7740

7681 acatttctct tcgttccttt cactttcgtt tctatctttt ccaaacctgt acagctgcag 7740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 ctctccaaat gaaatgaact tccttatata gagggaaggg tcttgcgaaa atagtgggat 7800

7741 gagaggttta ctttacttga aggaatatat ctcccttccc agaacgcttt tatcacccta 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 tgtgcgtcat cccttacgtc agtggaaata tcacatcaat ccacttgctt tgaagacgtg 7860

7801 acacgcagta gggaatgcag tcacctttat agtgtagtta ggtgaacgaa acttctgcac 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 gttggaacgt cttctttttc cacgatgttc ctcgtgggtg ggggtccatc tttgggacca 7920

7861 caaccttgca gaagaaaaag gtgctacaag gagcacccac ccccaggtag aaaccctggt 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ctgtcggtag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag 7980

7921 gacagccatc tccgtagaac ttgctatcgg aaaggaaata gcgttactac cgtaaacatc 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 aagccatctt ccttttctac tgtcctttcg atgaagtgac agatagctgg gcaatggaat 8040

7981 ttcggtagaa ggaaaagatg acaggaaagc tacttcactg tctatcgacc cgttacctta 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ccgaggaggt ttccgaatat taccctttgt tgaaaagtct caatagccct ttgaaaagtc 8100

8041 ggctcctcca aaggcttata atgggaaaca acttttcaga gttatcggga aacttttcag 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 tcaatagccc tctggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg 8160

8101 agttatcggg agaccagaag actctgacat agaaactata agaacctcat ctgctctcac 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 tcgtgctcca ccatgttgac ggatccttct cagcttggcg taatcatggt catagctgtt 8220

8161 agcacgaggt ggtacaactg cctaggaaga gtcgaaccgc attagtacca gtatcgacaa 8220

.......... .......... .......... .......... ...... M13_reverse_primer

.......... .......... .......... .......... .......... ..........

8221 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 8280

8221 aggacacact ttaacaatag gcgagtgtta aggtgtgttg tatgctcggc cttcgtattt 8280

...... M13_pUC_rev_primer ...... ......... lac_promoter .........

.......... .......... .......... .......... .......... ..........

8281 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt gcgctcactg 8340

8281 cacatttcgg accccacgga ttactcactc gattgagtgt aattaacgca cgcgagtgac 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 8400

8341 gggcgaaagg tcagcccttt ggacagcacg gtcgacgtaa ttacttagcc ggttgcgcgc 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 gggagaggcg gtttgcgtat tgggccaaag acaaaagggc gacattcaac cgattgaggg 8460

8401 ccctctccgc caaacgcata acccggtttc tgttttcccg ctgtaagttg gctaactccc 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 agggaaggta aatattgacg gaaattattc attaaaggtg aattatcacc gtcaccgact 8520

8461 tcccttccat ttataactgc ctttaataag taatttccac ttaatagtgg cagtggctga 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 tgagccattt gggaattaga gccagcaaaa tcaccagtag caccattacc attagcaagg 8580

8521 actcggtaaa cccttaatct cggtcgtttt agtggtcatc gtggtaatgg taatcgttcc 8580

.......... .......... .......... .......... .......... ..........

.......... .......... ClaI(8606) .......... .......... ..........

8581 ccggaaacgt caccaatgaa accatcgata gcagcaccgt aatcagtagc gacagaatca 8640

8581 ggcctttgca gtggttactt tggtagctat cgtcgtggca ttagtcatcg ctgtcttagt 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 agtttgcctt tagcgtcaga ctgtagcgcg ttttcatcgg cattttcggt catagccccc 8700

8641 tcaaacggaa atcgcagtct gacatcgcgc aaaagtagcc gtaaaagcca gtatcggggg 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 ttattagcgt ttgccatctt ttcataatca aaatcaccgg aaccagagcc accaccggaa 8760

8701 aataatcgca aacggtagaa aagtattagt tttagtggcc ttggtctcgg tggtggcctt 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 ccgcctccct cagagccgcc accctcagaa ccgccaccct cagagccacc accctcagag 8820

8761 ggcggaggga gtctcggcgg tgggagtctt ggcggtggga gtctcggtgg tgggagtctc 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 ccgccaccag aaccaccacc agagccgccg ccagcattga caggaggccc gatctagtaa 8880

8821 ggcggtggtc ttggtggtgg tctcggcggc ggtcgtaact gtcctccggg ctagatcatt 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat 8940

8881 gtatctactg tggcgcgcgc tattaaatag gatcaaacgc gcgatataaa acaaaagata 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt 9000

8941 gcgcataatt tacatattaa cgccctgaga ttagtatttt tgggtagagt atttattgca 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat 9060

9001 gtacgtaatg tacaattaat aatgtacgaa ttgcattaag ttgtctttaa tatactatta 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg 9120

9061 gtagcgttct ggccgttgtc ctaagttaga attctttgaa ataacggttt acaaacttgc 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 atcggggatc atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat 9180

9121 tagcccctag taggcccaga caccgccctt gaggtgcttt tataggcttg cgtcgttcta 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc 9240

9181 tagcgccacg tagagccaga acggacccgt cagcggcggc tgcggcaact acacctgcgg 9240

.......... .......... .......... .......... .......... ..........

ApaI(9246) .......... .......... .......... .......... ..........

9241 gggcccgatc atattgtcgc tcaggatcgt ggcgttgtgc ttgtcggccg ttgctgtcgt 9300

9241 cccgggctag tataacagcg agtcctagca ccgcaacacg aacagccggc aacgacagca 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 aatgatatcg gcaccttcga ccgcctgttc cgcagagatc ccgtgggcga agaactccag 9360

9301 ttactatagc cgtggaagct ggcggacaag gcgtctctag ggcacccgct tcttgaggtc 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc 9420

9361 gtactctagg ggcgcgacct cctagtaggt cggccgcagg gccttttgct aaggcttcgg 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc 9480

9421 gttggaaagt atcttccgcc gccaccttag ctttagagca ctaccgtcca acccgcagcg 9480

.......... .......... .......... .......... .......... ORF 5

.......... .......... .......... .......... .......... ..........

9481 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag 9540

9481 aaccagccag taaagcttgg ggtctcaggg cgagtcttct tgagcagttc ttccgctatc 9540

.......... .......... ORF 5 .......... nptII ..........

.......... .......... .......... .......... .......... ..........

9541 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc 9600

9541 ttccgctacg cgacgcttag ccctcgccgc tatggcattt cgtgctcctt cgccagtcgg 9600

.......... ......... ORF 5 nptII ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg 9660

9601 gtaagcggcg gttcgagaag tcgttatagt gcccatcggt tgcgatacag gactatcgcc 9660

.......... ......... ORF 5 nptII ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg 9720

9661 aggcggtgtg ggtcggccgg tgtcagctac ttaggtcttt tcgccggtaa aaggtggtac 9720

.......... ......... ORF 5 nptII ......... .......... ..........

.......... .......... NcoI(9743) .......... .......... ..........

9721 atattcggca agcaggcatc gccatgggtc acgacgagat catcgccgtc gggcatgcgc 9780

9721 tataagccgt tcgtccgtag cggtacccag tgctgctcta gtagcggcag cccgtacgcg 9780

.......... ......... ORF 5 nptII ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca 9840

9781 cggaactcgg accgcttgtc aagccgaccg cgctcgggga ctacgagaag caggtctagt 9840

.......... ......... ORF 5 nptII ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct 9900

9841 aggactagct gttctggccg aaggtaggct catgcacgag cgagctacgc tacaaagcga 9900

.......... ......... ORF 5 nptII ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc 9960

9901 accaccagct tacccgtcca tcggcctagt tcgcatacgt cggcggcgta acgtagtcgg 9960

.......... ......... ORF 5 nptII ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact 10020

9961 tactacctat gaaagagccg tcctcgttcc actctactgt cctctaggac ggggccgtga 10020

.......... ORF 5 nptII .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa 10080

10021 agcgggttat cgtcggtcag ggaagggcga agtcactgtt gcagctcgtg tcgacgcgtt 10080

.......... .......... nptII .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg 10140

10081 ccttgcgggc agcaccggtc ggtgctatcg gcgcgacgga gcaggacgtc aagtaagtcc 10140

.......... .......... nptII .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac 10200

10141 cgtggcctgt ccagccagaa ctgtttttct tggcccgcgg ggacgcgact gtcggccttg 10200

.......... .......... nptII .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc 10260

10201 tgccgccgta gtctcgtcgg ctaacagaca acacgggtca gtatcggctt atcggagagg 10260

.......... .......... nptII .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcca 10320

10261 tgggttcgcc ggcctcttgg acgcacgtta ggtagaacaa gttagtacgc tttgctaggt 10320

.......... .......... nptII .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 gatccggtgc agattatttg gattgagagt gaatatgaga ctctaattgg ataccgaggg 10380

10321 ctaggccacg tctaataaac ctaactctca cttatactct gagattaacc tatggctccc 10380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac 10440

10381 cttaaatacc ttgcagtcac ctcgtaaaaa ctgttcttta taaacgatcg actatcactg 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac 10500

10441 gaatccgctg aaaacttgcg cgttattacc aaagactgca tacacgaatc gagtaatttg 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta 10560

10501 aggtctttgg gcgccgactc accgaggaag ttgcaacgcc aagacagtca aggtttgcat 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc 10620

10561 tttgccgaac agggcgcagt agccgccccc agtattgcac tgagggaatt aagaggcgag 10620

.......... .......... .......... .......... .......... ..........

BclI(10623) ........ .......... .......... .......... ..........

10621 atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt 10680

10621 tactagtcta acagcaaagg gcggaagtca aatttgatag tcacaaactg tcctatataa 10680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt 10740

10681 ccgcccattt ggattctctt ttctcgcaaa taatcttatt agcctataaa ttttcccgca 10740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... BglII(10797)

10741 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc 10800

10741 cttttccaaa taggcaagca ggtaaacata cacgtacggt tggtgtccca aggggtctag 10800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 tggcgccggc cagcgagacg agcaagattg gccgccgccc gaaacgatcc gacagcgcgc 10860

10801 accgcggccg gtcgctctgc tcgttctaac cggcggcggg ctttgctagg ctgtcgcgcg 10860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 ccagcacagg tgcgcaggca aattgcacca acgcatacag cgccagcaga atgccatagt 10920

10861 ggtcgtgtcc acgcgtccgt ttaacgtggt tgcgtatgtc gcggtcgtct tacggtatca 10920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa 10980

10921 cccgccactg cagcaagctc acttggtcta gcgcgtcctc cgggccgtcg tggccgtatt 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 tcaggccgat gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtatgt 11040

10981 agtccggcta cggctgtcgc agctcgcgct gtcacgagtc ttaatgctag tccccataca 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 tgggtttcac gtctggcctc cggaccagcc tccgctggtc cgattgaacg cgcggattct 11100

11041 acccaaagtg cagaccggag gcctggtcgg aggcgaccag gctaacttgc gcgcctaaga 11100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11101 ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt caagcatgac 11160

11101 aatagtgact attcaaccac ctgtataata caaatagtca ctatttcaca gttcgtactg 11160

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

11161 aaagttgcag ccgaatacag tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt 11220

11161 tttcaacgtc ggcttatgtc actaggcacg gcgggacctg gacaacttgc tccagccgca 11220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11221 agacggtctg acgacacgca aactggcgga acggttgggg gttcagcagc cggcgcttta 11280

11221 tctgccagac tgctgtgcgt ttgaccgcct tgccaacccc caagtcgtcg gccgcgaaat 11280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 ctggcacttc aggaacaagc gggcgctgct cgacgcactg gccgaagcca tgctggcgga 11340

11281 gaccgtgaag tccttgttcg cccgcgacga gctgcgtgac cggcttcggt acgaccgcct 11340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11341 gaatcatacg cattcggtgc cgagagccga cgacgactgg cgctcatttc tgatcgggaa 11400

11341 cttagtatgc gtaagccacg gctctcggct gctgctgacc gcgagtaaag actagccctt 11400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11401 tgcccgcagc ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg 11460

11401 acgggcgtcg aagtccgtcc gcgacgagcg gatggcgcta ccgcgcgcgt aggtacggcc 11460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11461 cacgcgaccg ggcgcaccgc agatggaaac ggccgacgcg cagcttcgct tcctctgcga 11520

11461 gtgcgctggc ccgcgtggcg tctacctttg ccggctgcgc gtcgaagcga aggagacgct 11520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11521 ggcgggtttt tcggccgggg acgccgtcaa tgcgctgatg acaatcagct acttcactgt 11580

11521 ccgcccaaaa agccggcccc tgcggcagtt acgcgactac tgttagtcga tgaagtgaca 11580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11581 tggggccgtg cttgaggagc aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt 11640

11581 accccggcac gaactcctcg tccggccgct gtcgctacgg ccgctcgcgc cgccgtggca 11640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11641 tgaacaggct ccgctctcgc cgctgttgcg ggccgcgata gacgccttcg acgaagccgg 11700

11641 acttgtccga ggcgagagcg gcgacaacgc ccggcgctat ctgcggaagc tgcttcggcc 11700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11701 tccggacgca gcgttcgagc agggactcgc ggtgattgtc gatggattgg cgaaaaggag 11760

11701 aggcctgcgt cgcaagctcg tccctgagcg ccactaacag ctacctaacc gcttttcctc 11760

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11761 gctcgttgtc aggaacgttg aaggaccgag aaagggtgac gattgatcag gaccgctgcc 11820

11761 cgagcaacag tccttgcaac ttcctggctc tttcccactg ctaactagtc ctggcgacgg 11820

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11821 ggagcgcaac ccactcacta cagcagagcc atgtagacaa catcccctcc ccctttccac 11880

11821 cctcgcgttg ggtgagtgat gtcgtctcgg tacatctgtt gtaggggagg gggaaaggtg 11880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11881 cgcgtcagac gcccgtagca gcccgctacg ggctttttca tgccctgccc tagcgtccaa 11940

11881 gcgcagtctg cgggcatcgt cgggcgatgc ccgaaaaagt acgggacggg atcgcaggtt 11940

.......... .......... .......... .......... .......... ..........

EagI(11948) ........ .......... .......... .......... ..........

11941 gcctcacggc cgcgctcggc ctctctggcg gccttctggc gctcttccgc ttcctcgctc 12000

11941 cggagtgccg gcgcgagccg gagagaccgc cggaagaccg cgagaaggcg aaggagcgag 12000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 12060

12001 tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt gagtttccgc 12060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 12120

12061 cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac tcgttttccg 12120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 12180

12121 gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg 12180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 12240

12181 gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt gggctgtcct 12240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 12300

12241 gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg acaaggctgg 12300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gcttttccgc 12360

12301 gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg cgaaaaggcg 12360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12361 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc 12420

12361 acgtattggg acgaagcccc agtaatatcg ctaaaaaagc catataggta ggaaaaagcg 12420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12421 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg 12480

12421 tgctatatgt cctaaaacgg tttcccaagc acatctgaaa ggaaccacat aggttgccgc 12480

.......... .......... .......... .......... oriT_RP4 ..........

.......... .......... .......... .......... .......... ..........

12481 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt 12540

12481 agtcggcccg tcctatccac ttcatccggg tgggcgctcg cccacaagga agaagtgaca 12540

.......... .......... oriT_RP4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12541 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac 12600

12541 gggaataagc gtggaccgcc acgagttgcc cttaggacga gacgctccga ccggccgatg 12600

oriT_RP4 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12601 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg 12660

12601 gcggccgcat tgtctactcc cgttcgccta ccgactactt tggttcggtt ggtccttccc 12660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12661 cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc 12720

12661 gtcgggtgga tagttccaca tgacggaagg tctgcttgct tctcgctaac tccttttccg 12720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12721 ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat 12780

12721 ccgccgccgg ccgtactcgg acagccggat ggacgaccgg cagccggtcc cgatgtttta 12780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12781 cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg 12840

12781 gtgcccgcag cacctgatac tcgtgcaggc gctcgaccgg gcgtagttac cgctggaccc 12840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12841 ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg 12900

12841 ggcggacccg ccggacgact ttgagaccga gtggctgctg ggcgcgtgcc gcgccaagcc 12900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12901 tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa 12960

12901 actacggtgc taggagcggg acgaccgctt ctagcttctc ttcgtcctgc tcgaaccgtt 12960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EagI(13020)

12961 ggtcatgatg ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg 13020

12961 ccagtactac ccgcaccagg cgggctcccg tctcggtact gaaaaaatcg gcgattttgc 13020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13021 gccggggggt gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact 13080

13021 cggcccccca cgcgcactaa cggttcgtgc aggggtacgc gaggtagttc ttctcgctga 13080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13081 tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc ctttgcgacg 13140

13081 agcgcctcga ccacttcatg tagtggctgc tcgttccgtt ctggctcgcg gaaacgctgc 13140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13141 ctca...... .......... .......... .......... .......... .......... 13144

13141 gagt...... .......... .......... .......... .......... .......... 13144

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-13144 Reference author: Abdeeva I.A., Abdeev R.M., Piruzian E.S.; Reference title: ; Reference location: Submitted (15-JUL-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Laboratory of Functional Genomics, Vavilov's Institute of General Genetics Reference location: Russian Academy of Science, Gubkina, Moscow 119991, Russia