Sequence of pBK-CMV

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Sequence of pBK-CMV

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Sequence of pBK-CMV : 4518 BP; 1100 A; 1147 C; 1170 G; 1101 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 60

1 gtggactgcg cgggacatcg ccgcgtaatt cgcgccgccc acaccaccaa tgcgcgtcgc 60

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 120

61 actggcgatg tgaacggtcg cgggatcgcg ggcgaggaaa gcgaaagaag ggaaggaaag 120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 180

121 agcggtgcaa gcggccgaaa ggggcagttc gagatttagc ccccgaggga aatcccaagg 180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 240

181 ctaaatcacg aaatgccgtg gagctggggt tttttgaact aatcccacta ccaagtgcat 240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 300

241 cacccggtag cgggactatc tgccaaaaag cgggaaactg caacctcagg tgcaagaaat 300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 360

301 tatcacctga gaacaaggtt tgaccttgtt gtgagttggg atagagccag ataagaaaac 360

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 420

361 taaatattcc ctaaaacggc taaagccgga taaccaattt tttactcgac taaattgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtta agatacattg 480

421 ttaaattgcg cttaaaattg ttttataatt gcgaatgtta aatgcgcaat tctatgtaac 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 540

481 tactcaaacc tgtttggtgt tgatcttacg tcactttttt tacgaaataa acactttaaa 540

EBV_rev_pri ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 600

541 cactacgata acgaaataaa cattggtaat attcgacgtt atttgttcaa ttgttgttgt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt 660

601 taacgtaagt aaaatacaaa gtccaagtcc ccctccacac cctccaaaaa atttcgttca 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... BclI(692) .......... ..........

661 aaaacctcta caaatgtggt atggctgatt atgatcatga acagactgtg aggactgagg 720

661 ttttggagat gtttacacca taccgactaa tactagtact tgtctgacac tcctgactcc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcctgaaat gagccttggg actgtgaatc taaaatacac aaacaattag aatcagtagt 780

721 ccggacttta ctcggaaccc tgacacttag attttatgtg tttgttaatc ttagtcatca 780

.......... .......... .......... ........ SV40_3_splice ........

.......... .......... .......... .......... .......... ..........

781 ttaacacatt atacacttaa aaattggatc tccattcgcc attcaggctg cgcaactgtt 840

781 aattgtgtaa tatgtgaatt tttaacctag aggtaagcgg taagtccgac gcgttgacaa 840

........ SV40_3_splice ........ .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

841 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 900

841 cccttcccgc tagccacgcc cggagaagcg ataatgcggt cgaccgcttt ccccctacac 900

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 960

901 gacgttccgc taattcaacc cattgcggtc ccaaaagggt cagtgctgca acattttgct 960

.......... .......... lacZ_a ... M13_pUC_fwd_pri M13_forward20_pri

.......... .......... .......... .......... .......... ..........

961 cggccagtga attgtaatac gactcactat agggcgaatt gggtacactt acctggtacc 1020

961 gccggtcact taacattatg ctgagtgata tcccgcttaa cccatgtgaa tggaccatgg 1020

lacZ_a .......... T7_pro .......... .......... ..........

SmaI ApaI,SacII),NotI(1049) XbaI(1056) XhoI(1068)

1021 ccacccgggt ggaaaatcga tgggcccgcg gccgctctag aagtactctc gagaagcttt 1080

1021 ggtgggccca ccttttagct acccgggcgc cggcgagatc ttcatgagag ctcttcgaaa 1080

.......... .......... .......... .......... .......... ..........

EcoRI BamHI SpeI SalI(1104) PstI(1114) SacI(1126)

1081 ttgaattctt tggatccact agtgtcgacc tgcaggcgcg cgagctccag cttttgttcc 1140

1081 aacttaagaa acctaggtga tcacagctgg acgtccgcgc gctcgaggtc gaaaacaagg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctttagtgag ggttaatttc gagcttggcg taatcaaggt catagctgtt tcctgtgtga 1200

1141 gaaatcactc ccaattaaag ctcgaaccgc attagttcca gtatcgacaa aggacacact 1200

T3_pro .......... .......... .......... ....... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

1201 aattgttatc cgctcacaat tccacacaat atacgagccg gaagtataaa gtgtaaagcc 1260

1201 ttaacaatag gcgagtgtta aggtgtgtta tatgctcggc cttcatattt cacatttcgg 1260

M13_pUC_rev_pri .... .......... .......... .......... ..........

.......... .......... .......... NheI(1300) .......... ..........

1261 tggggtgcct aatgagtgag ctaactcaca gtaattgcgg ctagcggatc tgacggttca 1320

1261 accccacgga ttactcactc gattgagtgt cattaacgcc gatcgcctag actgccaagt 1320

.......... .......... .......... .......... .......... CMV_pro

.......... .......... .......... .......... .......... ..........

1321 ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca 1380

1321 gatttggtcg agacgaatat atctggaggg tggcatgtgc ggatggcggg taaacgcagt 1380

.......... ...... CMV_pro CMV_immearly_pro CMV_fwd_pri ......

.......... .......... .......... .......... .......... ..........

1381 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac 1440

1381 taccccgcct caacaatgct gtaaaacctt tcagggcaac taaaaccacg gttttgtttg 1440

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac 1500

1441 agggtaactg cagttacccc acctctgaac ctttaggggc actcagtttg gcgataggtg 1500

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga 1560

1501 cgggtaacta catgacggtt ttggcgtagt ggtaccatta tcgctactga ttatgcatct 1560

.......... ....... CMV_immearly_pro ....... .......... CAG_enh

.......... .......... .......... .......... .......... ..........

1561 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc 1620

1561 acatgacggt tcatcctttc agggtattcc agtacatgac ccgtattacg gtccgcccgg 1620

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... NdeI(1659) .......... ..........

1621 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg 1680

1621 taaatggcag taactgcagt tatcccccgc atgaaccgta tactatgtga actacatgac 1680

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg 1740

1681 ggttcacccg tcaaatggca tttatgaggt gggtaactgc agttaccttt cagggataac 1740

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc 1800

1741 cgcaatgata cccttgtatg cagtaataac tgcagttacc cgcccccagc aacccgccag 1800

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa 1860

1801 tcggtccgcc cggtaaatgg cattcaatac attgcgcctt gaggtatata cccgatactt 1860

....... CAG_enh CMV_immearly_pro ....... .......... ..........

.......... .......... AseI(1886) .......... .......... ..........

1861 ctaatgaccc cgtaattgat tactattaat aactaatgca tggcggtaat acggttatcc 1920

1861 gattactggg gcattaacta atgataatta ttgattacgt accgccatta tgccaatagg 1920

....... CMV_immearly_pro ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 1980

1921 tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2040

1981 ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta 2040

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2100

2041 gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2160

2101 cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2220

2161 atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... ......... ApaLI(2263) .........

2221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2280

2221 atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2340

2281 gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2400

2341 ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 2460

2401 ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc ctgtcataaa 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2520

2461 ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2580

2521 ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2640

2581 tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc 2640

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 2700

2641 ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta gaagtggatc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaacctgag 2760

2701 taggaaaatt taatttttac ttcaaaattt agttagattt catatatact cattggactc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt 2820

2761 cgataccgtc ccggacggcg gggctgcaac cgacgctcgg gacccggaag tgggcttgaa 2820

.......... .......... .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt 2880

2821 ccccccaccc cacccctttt ccttctttgc gcccgcataa ccggggttac cccagagcca 2880

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca 2940

2881 ccccatagct gtctcacggt cgggaccctg gcttggggcg caaatacttg tttgctgggt 2940

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt 3000

2941 tgtggcacgc aaaataagac agaaaaataa cggcagtatc gcgcccaagg aaggccataa 3000

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga 3060

3001 cagaggaagg cacaaagtca atcggagggg gatcccaccc gcttcttgag gtcgtactct 3060

.......... TK_PA_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt 3120

3061 aggggcgcga cctcctagta ggtcggccgc agggcctttt gctaaggctt cgggttggaa 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg 3180

3121 agtatcttcc gccgccacct tagctttaga gcactaccgt ccaacccgca gcgaaccagc 3180

.......... .......... .......... .......... .......... ..........

BstBI(3187) ........ .......... .......... .......... ..........

3181 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga 3240

3181 cagtaaagct tggggtctca gggcgagtct tcttgagcag ttcttccgct atcttccgct 3240

.......... .......... .......... ... ORF 2 Neomycin(variant) ...

.......... .......... .......... .......... .......... ..........

3241 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc 3300

3241 acgcgacgct tagccctcgc cgctatggca tttcgtgctc cttcgccagt cgggtaagcg 3300

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca 3360

3301 gcggttcgag aagtcgttat agtgcccatc ggttgcgata caggactatc gccaggcggt 3360

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg 3420

3361 gtgggtcggc cggtgtcagc tacttaggtc ttttcgccgg taaaaggtgg tactataagc 3420

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga 3480

3421 cgttcgtccg tagcggtacc cagtgctgct ctaggagcgg cagcccgtac gagcggaact 3480

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat 3540

3481 cggaccgctt gtcaagccga ccgcgctcgg ggactacgag aagcaggtct agtaggacta 3540

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt 3600

3541 gctgttctgg ccgaaggtag gctcatgcac gagcgagcta cgctacaaag cgaaccacca 3600

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg 3660

3601 gcttacccgt ccatcggcct agttcgcata cgtcggcggc gtaacgtagt cggtactacc 3660

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca 3720

3661 tatgaaagag ccgtcctcgt tccactctac tgtcctctag gacggggccg tgaagcgggt 3720

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc 3780

3721 tatcgtcggt cagggaaggg cgaagtcact gttgcagctc gtgtcgacgc gttccttgcg 3780

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg 3840

3781 ggcagcaccg gtcggtgcta tcggcgcgac ggagcagaac gtcaagtaag tcccgtggcc 3840

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... NarI(3869) .......... .......... ..........

3841 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg 3900

3841 tgtccagcca gaactgtttt tcttggcccg cggggacgcg actgtcggcc ttgtgccgcc 3900

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag 3960

3901 gtagtctcgt cggctaacag acaacacggg tcagtatcgg cttatcggag aggtgggttc 3960

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg 4020

3961 gccggcctct tggacgcacg ttaggtagaa caagttagta cgctttgcta ggagtaggac 4020

... ORF 2 Neomycin(variant) ... .......... .......... ..........

.......... .......... StuI(4050) .......... .......... ..........

4021 tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg 4080

4021 agagaactag ctagaaacgt tttcggatcc ggaggttttt tcggaggagt gatgaagacc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca 4140

4081 ttatcgagtc tccggctccg ccggagccgg agacgtattt atttttttta atcagtcggt 4140

.......... .......... ... SV40_pro SV40pro_F_pri SV40_origin ...

.......... .......... .......... .......... .......... ..........

4141 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc 4200

4141 accccgcctc ttacccgcct tgacccgcct caatccccgc cctacccgcc tcaatccccg 4200

.......... ..... SV40_pro SV40_origin SV40_enh ..... ..........

.......... .......... .......... .......... .......... ..........

4201 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag 4260

4201 ccctgatacc aacgactgat taactctacg tacgaaacgt atgaagacgg acgacccctc 4260

.......... ...... SV40_pro SV40_enh ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg 4320

4261 ggacccctga aaggtgtgga ccaacgactg attaactcta cgtacgaaac gtatgaagac 4320

.......... ...... SV40_pro SV40_enh ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc 4380

4321 ggacgacccc tcggacccct gaaaggtgtg ggattgactg tgtgtaaggt gtcgaccaag 4380

......... SV40_pro SV40_enh pBABE_3_pri ......... ..........

.......... .......... .......... .......... .......... ..........

4381 tttccgcctc aggactcttc ctttttcaat attattgaag catttatcag ggttattgtc 4440

4381 aaaggcggag tcctgagaag gaaaaagtta taataacttc gtaaatagtc ccaataacag 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 4500

4441 agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc caaggcgcgt 4500

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 catttccccg aaaagtgc.. .......... .......... .......... .......... 4518

4501 gtaaaggggc ttttcacg.. .......... .......... .......... .......... 4518

.......... .......... .......... .......... .......... ..........