Sequence of pBOB-CAG-iCRE-SD

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Sequence of pBOB-CAG-iCRE-SD

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Sequence of pBOB-CAG-iCRE-SD : 10714 BP; 2470 A; 2724 C; 3002 G; 2518 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ......... CMV_imm_pro CAG_enh ......... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ......... CMV_imm_pro ......... .......... CMV_f

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc 840

781 catccgcaca tgccaccctc cagatatatt cgtcgcgcaa aacggacatg acccagagag 840

CMV_f CMV_imm_pro ........ .......... ......... HIV truncHIV

.......... .......... .......... .......... .......... ..........

841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 900

841 accaatctgg tctagactcg gaccctcgag agaccgattg atcccttggg tgacgaattc 900

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 960

901 ggagttattt cgaacggaac tcacgaagtt catcacacac gggcagacaa cacactgaga 960

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 1020

961 ccattgatct ctagggagtc tgggaaaatc agtcacacct tttagagatc gtcaccgcgg 1020

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg 1080

1021 gcttgtccct ggactttcgc tttccctttg gtctcgagag agctgcgtcc tgagccgaac 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 1140

1081 gacttcgcgc gtgccgttct ccgctccccg ccgctgacca ctcatgcggt ttttaaaact 1140

.......... .......... .......... .......... HIV-1 ..........

.......... .......... .......... .......... .......... ..........

1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 1200

1141 gatcgcctcc gatcttcctc tctctaccca cgctctcgca gtcataattc gccccctctt 1200

.......... HIV-1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 1260

1201 aatctagcgc tacccttttt taagccaatt ccggtccccc tttctttttt atatttaatt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 1320

1261 ttgtatatca tacccgttcg tccctcgatc ttgctaagcg tcaattagga ccggacaatc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 1380

1321 tttgtagtct tccgacatct gtttatgacc ctgtcgatgt tggtagggaa gtctgtccta 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1440

1381 gtcttcttga atctagtaat atattatgtc atcgttggga gataacacac gtagtttcct 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1500

1441 atctctattt tctgtggttc cttcgaaatc tgttctatct ccttctcgtt ttgttttcat 1500

.......... .......... .......... .......... .......... ..........

.......... NotI(1519) .......... .......... .......... ..........

1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac 1560

1501 tctggtggcg tgtcgttcgc cggcgactag aagtctgaac ctcctcctct atactccctg 1560

.......... .......... .......... .......... .......... ORF 1

.......... .......... .......... .......... .......... ..........

1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1620

1561 ttaacctctt cacttaatat atttatattt catcattttt aacttggtaa tcctcatcgt 1620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1680

1621 gggtggttcc gtttctcttc tcaccacgtc tctctttttt ctcgtcaccc ttatcctcga 1680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg 1740

1681 aacaaggaac ccaagaaccc tcgtcgtcct tcgtgatacc cgcgtcggag ttactgcgac 1740

.......... .......... ORF 1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1800

1741 tgccatgtcc ggtctgttaa taacagacca tatcacgtcg tcgtcttgtt aaacgactcc 1800

.......... .......... ORF 1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa 1860

1801 cgataactcc gcgttgtcgt agacaacgtt gagtgtcaga ccccgtagtt cgtcgaggtt 1860

.......... .......... ORF 1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt 1920

1861 cgttcttagg atcgacacct ttctatggat ttcctagttg tcgaggatcc ctaaacccca 1920

.......... .......... ORF 1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1980

1921 acgagacctt ttgagtaaac gtggtgacga cacggaacct tacgatcaac ctcattattt 1980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat 2040

1981 agagaccttg tctagacctt agtgtgctgg acctacctca ccctgtctct ttaattgtta 2040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 2100

2041 atgtgttcga attatgtgag gaattaactt cttagcgttt tggtcgttct tttcttactt 2100

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 2160

2101 gttcttaata accttaatct atttacccgt tcaaacacct taaccaaatt gtattgttta 2160

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 2220

2161 accgacacca tatattttaa taagtattac tatcatcctc cgaaccatcc aaattcttat 2220

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 2280

2221 caaaaacgac atgaaagata tcacttatct caatccgtcc ctataagtgg taatagcaaa 2280

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 2340

2281 gtctgggtgg agggttaggg ctcccctggg ctgtccgggc ttccttatct tcttcttcca 2340

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa 2400

2341 cctctctctc tgtctctgtc taggtaagct aatcacttgc ctagttgaaa attttctttt 2400

.......... .......... ORF 1 .......... .......... cPPT

.......... .......... .......... .......... .......... ..........

2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata 2460

2401 cccccctaac cccccatgtc acgtcccctt tcttatcatc tgtattatcg ttgtctgtat 2460

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... ClaI(2507) ..........

2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgtcgacatt 2520

2461 gtttgatttc ttaatgtttt tgtttaatgt ttttaagttt taaaatagct acagctgtaa 2520

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 2580

2521 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 2580

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 2640

2581 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 2640

.......... .......... .......... CAG_enh .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 2700

2641 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 2700

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 2760

2701 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 2760

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 2820

2761 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 2820

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 2880

2821 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 2880

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcgctattac catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc 2940

2881 agcgataatg gtacccagct ccactcgggg tgcaagacga agtgagaggg gtagaggggg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg 3000

2941 gggaggggtg ggggttaaaa cataaataaa taaaaaatta ataaaacacg tcgctacccc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 3060

3001 cgcccccccc ccccccgcgc gcggtccgcc ccgccccgcc ccgctccccg ccccgccccg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 3120

3061 ctccgcctct ccacgccgcc gtcggttagt ctcgccgcgc gaggctttca aaggaaaata 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc 3180

3121 ccgctccgcc gccgccgccg ccgggatatt tttcgcttcg cgcgccgccc gccctcagcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg 3240

3181 acgcaacgga agcggggcac ggggcgaggc gcggcggagc gcggcgggcg gggccgagac 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 3300

3241 tgactggcgc aatgagggtg tccactcgcc cgccctgccg ggaagaggag gcccgacatt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg 3360

3301 aatcgcgaac caaattactg ccgagcaaag aaaagacacc gacgcacttt cggaatttcc 3360

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt 3420

3361 cgaggccctc ccgggaaaca cgccccccct cgccgagccc cccacgcacg cacacacaca 3420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg 3480

3421 cgcacccctc gcggcgcacg ccgggcgcga cgggccgccg acactcgcga cgcccgcgcc 3480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg 3540

3481 gcgccccgaa acacgcgagg cgcacacgcg ctcccctcgc gccggccccc gccacggggc 3540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt 3600

3541 gccacgcccc cccgacgctc cccttgtttc cgacgcacgc cccacacacg caccccccca 3600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga 3660

3601 ctcgtccccc acacccgcgc cgccagcccg acattggggg gggacgtggg gggaggggct 3660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc 3720

3661 caacgactcg tgccgggccg aagcccacgc cccgaggcac gccccgcacc gcgccccgag 3720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg 3780

3721 cggcacggcc cgccccccac cgccgtccac ccccacggcc cgccccgccc cggcggagcc 3780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gccggggagg gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg 3840

3781 cggcccctcc cgagccccct ccccgcgccg ccggggcctc gcggccgccg acagctccgc 3840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt 3900

3841 gccgctcggc gtcggtaacg gaaaatacca ttagcacgct ctcccgcgtc cctgaaggaa 3900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg 3960

3901 acagggttta gaccgcctcg gctttagacc ctccgcggcg gcgtggggga gatcgcccgc 3960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg 4020

3961 gcccgcttcg ccacgccgcg gccgtccttc ctttacccgc ccctcccgga agcacgcagc 4020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccgcgccgcc gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc 4080

4021 ggcgcggcgg caggggaaga ggtagaggtc ggagccccga cggcgtcccc ctgccgacgg 4080

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... XbaI(4134)

4081 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 4140

4081 aagccccccc tgccccgtcc cgccccaagc cgaagaccgc acactggccg ccgagatctc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 4200

4141 ggagacgatt ggtacaagta cggaagaaga aaaaggatgt cgaggacccg ttgcacgacc 4200

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... ......... BamHI(4242) PstI(4258)

4201 ttgttgtgct gtctcatcat tttggcaaag aattcgctag tggatccccc gggctgcagg 4260

4201 aacaacacga cagagtagta aaaccgtttc ttaagcgatc acctaggggg cccgacgtcc 4260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aattcgatat caagcttgtc caccatggtg cccaagaaga agaggaaagt ctccaacctg 4320

4261 ttaagctata gttcgaacag gtggtaccac gggttcttct tctcctttca gaggttggac 4320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctgactgtgc accaaaacct gcctgccctc cctgtggatg ccacctctga tgaagtcagg 4380

4321 gactgacacg tggttttgga cggacgggag ggacacctac ggtggagact acttcagtcc 4380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aagaacctga tggacatgtt cagggacagg caggccttct ctgaacacac ctggaagatg 4440

4381 ttcttggact acctgtacaa gtccctgtcc gtccggaaga gacttgtgtg gaccttctac 4440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc 4500

4441 gaggacagac acacgtctag gacccgacgg accacgttcg acttgttgtc ctttaccaag 4500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cctgctgaac ctgaggatgt gagggactac ctcctgtacc tgcaagccag aggcctggct 4560

4501 ggacgacttg gactcctaca ctccctgatg gaggacatgg acgttcggtc tccggaccga 4560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtgaagacca tccaacagca cctgggccag ctcaacatgc tgcacaggag atctggcctg 4620

4561 cacttctggt aggttgtcgt ggacccggtc gagttgtacg acgtgtcctc tagaccggac 4620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat 4680

4621 ggagcgggaa gactgaggtt acgacacagg gaccactact cctcttagtc tttcctctta 4680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtggatgctg gggagagagc caagcaggcc ctggcctttg aacgcactga ctttgaccaa 4740

4681 cacctacgac ccctctctcg gttcgtccgg gaccggaaac ttgcgtgact gaaactggtt 4740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtcagatccc tgatggagaa ctctgacaga tgccaggaca tcaggaacct ggccttcctg 4800

4741 cagtctaggg actacctctt gagactgtct acggtcctgt agtccttgga ccggaaggac 4800

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggcattgcct acaacaccct gctgcgcatt gccgaaattg ccagaatcag agtgaaggac 4860

4801 ccgtaacgga tgttgtggga cgacgcgtaa cggctttaac ggtcttagtc tcacttcctg 4860

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atctcccgca ccgatggtgg gagaatgctg atccacattg gcaggaccaa gaccctggtg 4920

4861 tagagggcgt ggctaccacc ctcttacgac taggtgtaac cgtcctggtt ctgggaccac 4920

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tccacagctg gtgtggagaa ggccctgtcc ctgggggtta ccaagctggt ggagagatgg 4980

4921 aggtgtcgac cacacctctt ccgggacagg gacccccaat ggttcgacca cctctctacc 4980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atctctgtgt ctggtgtggc tgatgacccc aacaactacc tgttctgccg ggtcagaaag 5040

4981 tagagacaca gaccacaccg actactgggg ttgttgatgg acaagacggc ccagtctttc 5040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct ggaagggatc 5100

5041 ttaccacacc gacggggaag acggtggagg gttgacaggt gggcccggga ccttccctag 5100

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca gagatacctg 5160

5101 aaactccggt gggtggcgga ctagatacca cggttcctac tgagacccgt ctctatggac 5160

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gcctggtctg gccactctgc cagagtgggt gctgccaggg acatggccag ggctggtgtg 5220

5161 cggaccagac cggtgagacg gtctcaccca cgacggtccc tgtaccggtc ccgaccacac 5220

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacattgt gatgaactac 5280

5221 aggtagggac tttagtacgt ccgaccaccg acctggttac acttgtaaca ctacttgatg 5280

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... XhoI(5323) ..........

5281 atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgagga tggggactga 5340

5281 tagtctttgg acctgagact ctgaccccgg taccactccg acgagctcct acccctgact 5340

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aactgagtcg agggggggcc cgaaacctgg aaaaacatgg agcaatcaca agtagcaaca 5400

5341 ttgactcagc tcccccccgg gctttggacc tttttgtacc tcgttagtgt tcatcgttgt 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cagcagctac caatgctgct tgtgcctggc tagaagcaca agaggaggag gaggtgggtt 5460

5401 gtcgtcgatg gttacgacga acacggaccg atcttcgtgt tctcctcctc ctccacccaa 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ttccagtcac acctcagaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 5520

5461 aaggtcagtg tggagtctgt tagttggaga cctaatgttt taaacacttt ctaactgacc 5520

.......... .......... .......... .......... WPRE ..........

.......... .......... .......... .......... .......... ..........

5521 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 5580

5521 ataagaattg atacaacgag gaaaatgcga tacacctatg cgacgaaatt acggaaacat 5580

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 5640

5581 agtacgataa cgaagggcat accgaaagta aaagaggagg aacatattta ggaccaacga 5640

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 5700

5641 cagagaaata ctcctcaaca ccgggcaaca gtccgttgca ccgcaccaca cgtgacacaa 5700

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 5760

5701 acgactgcgt tgggggtgac caaccccgta acggtggtgg acagtcgagg aaaggccctg 5760

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 5820

5761 aaagcgaaag ggggagggat aacggtgccg ccttgagtag cggcggacgg aacgggcgac 5820

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 5880

5821 gacctgtccc cgagccgaca acccgtgact gttaaggcac cacaacagcc ccttcgactg 5880

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 5940

5881 caggaaaggt accgacgagc ggacacaacg gtggacctaa gacgcgccct gcaggaagac 5940

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 6000

5941 gatgcaggga agccgggagt taggtcgcct ggaaggaagg gcgccggacg acggccgaga 6000

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 6060

6001 cgccggagaa ggcgcagaag cggaagcggg agtctgctca gcctagaggg aaacccggcg 6060

.......... .......... WPRE .......... .......... ..........

.......... .......... KpnI(6088) .......... .......... ..........

6061 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta 6120

6061 gaggggcgga ccttaagctc gagccatgga aattctggtt actgaatgtt ccgtcgacat 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga 6180

6121 ctagaatcgg tgaaaaattt tcttttcccc cctgaccttc ccgattaagt gagggtttct 6180

.......... .......... cPPT U3PPT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattgacag 6240

6181 tctgttctat aggaactaga cacctagatg gtgtgtgttc cgatgaaggg actaactgtc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta 6300

6241 ttgatgtgtg gtcccggtcc ccagtctata ggtgactgga aacctaccac gatgttcgat 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gtaccagttg agccagataa gatagaagag gccaataaag gagagaacac cagcttgtta 6360

6301 catggtcaac tcggtctatt ctatcttctc cggttatttc ctctcttgtg gtcgaacaat 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt 6420

6361 gtgggacact cggacgtacc ctacctactg ggcctctctc ttcacaatct cacctccaaa 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggcata acttcgtata 6480

6421 ctgtcggcgg atcgtaaagt agtgcaccgg gctctcgacg taggccgtat tgaagcatat 6480

.......... .......... .......... .......... .......... loxP

.......... .......... .......... .......... .......... ..........

6481 gcatacatta tacgaagtta tgccggactg tactgggtct ctctggttag accagatc tg 6540

6481 cgtatgtaat atgcttcaat acggcctgac atgacccaga gagaccaatc tggtctagac 6540

.......... loxP .......... ......... HIV truncHIV .........

.......... .......... .......... .......... .......... ..........

6541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6600

6541 tcggaccctc gagagaccga ttgatccctt gggtgacgaa ttcggagtta tttcgaacgg 6600

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6660

6601 aactcacgaa gttcatcaca cacgggcaga caacacactg agaccattga tctctaggga 6660

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc cgctgatcag 6720

6661 gtctgggaaa atcagtcaca ccttttagag atcgtcccgg cgaaatttgg gcgactagtc 6720

......... HIV truncHIV ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 6780

6721 ggagctgaca cggaagatca acggtcggta gacaacaaac ggggaggggg cacggaagga 6780

BGH_r .......... ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

6781 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 6840

6781 actgggacct tccacggtga gggtgacagg aaaggattat tttactcctt taacgtagcg 6840

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 6900

6841 taacagactc atccacagta agataagacc ccccacccca ccccgtcctg tcgttccccc 6900

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 6960

6901 tcctaaccct tctgttatcg tccgtacgac ccctacgcca cccgagatac cgaagactcc 6960

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 7020

6961 gcctttcttg gtcgaccccg agatccccca taggggtgcg cgggacatcg ccgcgtaatt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 7080

7021 cgcgccgccc acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg cgggatcgcg 7080

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 7140

7081 ggcgaggaaa gcgaaagaag ggaaggaaag agcggtgcaa gcggccgaaa ggggcagttc 7140

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 7200

7141 gagatttagc cccgtaggga aatcccaagg ctaaatcacg aaatgccgtg gagctggggt 7200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 7260

7201 tttttgaact aatcccacta ccaagtgcat cacccggtag cgggactatc tgccaaaaag 7260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 7320

7261 cgggaaactg caacctcagg tgcaagaaat tatcacctga gaacaaggtt tgaccttgtt 7320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 7380

7321 gtgagttggg atagagccag ataagaaaac taaatattcc ctaaaacccc taaagccgga 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 7440

7381 taaccaattt tttactcgac taaattgttt ttaaattgcg cttaattaag acaccttaca 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca 7500

7441 cacagtcaat cccacacctt tcaggggtcc gaggggtccg tccgtcttca tacgtttcgt 7500

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 7560

7501 acgtagagtt aatcagtcgt tggtccacac ctttcagggg tccgaggggt cgtccgtctt 7560

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 7620

7561 catacgtttc gtacgtagag ttaatcagtc gttggtatca gggcggggat tgaggcgggt 7620

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 7680

7621 agggcgggga ttgaggcggg tcaaggcggg taagaggcgg ggtaccgact gattaaaaaa 7680

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 7740

7681 aataaatacg tctccggctc cggcggagac ggagactcga taaggtcttc atcactcctc 7740

SV40_origin SV40pro_F SV40_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 7800

7741 cgaaaaaacc tccggatccg aaaacgtttt tcgagggccc tcgaacatat aggtaaaagc 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 7860

7801 ctagactagt cgtgcacaac tgttaattag tagccgtatc atatagccgt atcatattat 7860

.......... .......... .......... EM7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

7861 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 7920

7861 gctgttccac tccttgattt ggtaccggtt caactggtca cggcaaggcc acgagtggcg 7920

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

7921 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 7980

7921 cgcgctgcag cggcctcgcc agctcaagac ctggctggcc gagcccaaga gggccctgaa 7980

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 8040

7981 gcacctcctg ctgaagcggc cacaccaggc cctgctgcac tgggacaagt agtcgcgcca 8040

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 8100

8041 ggtcctggtc caccacggcc tgttgtggga ccggacccac acccacgcgc cggacctgct 8100

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 8160

8101 cgacatgcgg ctcaccagcc tccagcacag gtgcttgaag gccctgcgga ggcccggccg 8160

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 8220

8161 gtactggctc tagccgctcg tcggcacccc cgccctcaag cgggacgcgc tgggccggcc 8220

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc 8280

8221 gttgacgcac gtgaagcacc ggctcctcgt cctgactgtg cacgatgctc taaagctaag 8280

......... bleo sh_ble ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 8340

8281 gtggcggcgg aagatacttt ccaacccgaa gccttagcaa aaggccctgc ggccgaccta 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 8400

8341 ctaggaggtc gcgcccctag agtacgacct caagaagcgg gtggggttga acaaataacg 8400

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

8401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 8460

8401 tcgaatatta ccaatgttta tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa 8460

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 8520

8461 aagtgacgta agatcaacac caaacaggtt tgagtagtta catagaatag tacagacata 8520

.......... ...... SV40_PA_term EBV_r ...... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 8580

8521 tggcagctgg agatcgatct cgaaccgcat tagtaccagt atcgacaaag gacacacttt 8580

.......... .......... .......... .......... M13_r M13_pUC_r

.......... .......... .......... .......... .......... ..........

8581 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 8640

8581 aacaataggc gagtgttaag gtgtgttgta tgctcggcct tcgtatttca catttcggac 8640

M13_pUC_r .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

8641 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 8700

8641 cccacggatt actcactcga ttgagtgtaa ttaacgcaac gcgagtgacg ggcgaaaggt 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 8760

8701 cagccctttg gacagcacgg tcgacgtaat tacttagccg gttgcgcgcc cctctccgcc 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8820

8761 aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg agcgacgcga gccagcaagc 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8880

8821 cgacgccgct cgccatagtc gagtgagttt ccgccattat gccaataggt gtcttagtcc 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8940

8881 cctattgcgt cctttcttgt acactcgttt tccggtcgtt ttccggtcct tggcattttt 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 9000

8941 ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag tgtttttagc 9000

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

9001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 9060

9001 tgcgagttca gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg 9060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 9120

9061 accttcgagg gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg 9120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9121 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 9180

9121 gaaagaggga agcccttcgc accgcgaaag agttacgagt gcgacatcca tagagtcaag 9180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 9240

9181 ccacatccag caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc 9240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 9300

9241 gacgcggaat aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg 9300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 9360

9301 tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct 9360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 9420

9361 caagaacttc accaccggat tgatgccgat gtgatcttcc tgtcataaac catagacgcg 9420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 9480

9421 agacgacttc ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg 9480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 9540

9481 gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc 9540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 9600

9541 tagagttctt ctaggaaact agaaaagatg ccccagactg cgagtcacct tgcttttgag 9600

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 9660

9601 tgcaattccc taaaaccagt actctaatag tttttcctag aagtggatct aggaaaattt 9660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 9720

9661 aatttttact tcaaaattta gttagatttc atatatactc atttgaacca gactgtcaat 9720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9780

9721 ggttacgaat tagtcactcc gtggatagag tcgctagaca gataaagcaa gtaggtatca 9780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9840

9781 acggactgag gggcagcaca tctattgatg ctatgccctc ccgaatggta gaccggggtc 9840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9900

9841 acgacgttac tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc gttatttggt 9900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9960

9901 cggtcggcct tcccggctcg cgtcttcacc aggacgttga aataggcgga ggtaggtcag 9960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 10020

9961 ataattaaca acggcccttc gatctcattc atcaagcggt caattatcaa acgcgttgca 10020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 10080
gaagtaagtc
10021 acaacggtaa cgatgtccgt agcaccacag tgcgagcagc aaaccatacc 10080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 10140
tttttcgcca
10081 gaggccaagg gttgctagtt ccgctcaatg tactaggggg tacaacacgt 10140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 10200
atagtgagta
10141 atcgaggaag ccaggaggct agcaacagtc ttcattcaac cggcgtcaca 10200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 10260
cgaaaagaca
10201 ccaataccgt cgtgacgtat taagagaatg acagtacggt aggcattcta 10260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 10320
gctcaacgag
10261 ctgaccactc atgagttggt tcagtaagac tcttatcaca tacgccgctg 10320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 10380
ttcacgagta
10321 aacgggccgc agttatgccc tattatggcg cggtgtatcg tcttgaaatt 10380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 10440
actctaggtc
10381 gtaacctttt gcaagaagcc ccgcttttga gagttcctag aatggcgaca 10440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 10500
agtggtcgca
10441 aagctacatt gggtgagcac gtgggttgac tagaagtcgt agaaaatgaa 10500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 10560
cccgctgtgc
10501 aagacccact cgtttttgtc cttccgtttt acggcgtttt ttcccttatt 10560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 10620

10561 ctttacaact tatgagtatg agaaggaaaa agttataata acttcgtaaa tagtcccaat 10620

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 10680

10621 aacagagtac tcgcctatgt ataaacttac ataaatcttt ttatttgttt atccccaagg 10680

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 gcgcacattt ccccgaaaag tgccacctga cgtc...... .......... .......... 10714

10681 cgcgtgtaaa ggggcttttc acggtggact gcag...... .......... .......... 10714

.......... .......... .......... .......... .......... ..........