Ys%Ys%ID EU573187; SV 1; circular; other DNA; STD; SYN; 2946 BP. AC EU573187; DT 22-JUN-2008 (Rel. 96, Created) DT 22-JUN-2008 (Rel. 96, Last updated, Version 1) DE Cloning vector pBS-SK-Sfi, complete sequence. KW . OS Cloning vector pBS-SK-Sfi OC other sequences; artificial sequences; vectors. RN [1] RP 1-2946 RA Sreenivasan R., Cai M., Bartfai R., Wang X., Christoffels A., Orban L.; RT "Transcriptomic analysis reveal novel genes with sexually dimorphic RT expression in the zebrafish gonad and brain"; RL PLoS One 3(1791):1-16(2008). RN [2] RP 1-2946 RA Sreenivasan R., Cai M., Bartfai R., Wang X., Christoffels A., Orban L.; RT ; RL Submitted (19-MAR-2008) to the EMBL/GenBank/DDBJ databases. RL Reproductive Genomics Laboratory, Temasek LifeSciences Laboratory, RL Singapore 117604, Singapore FH Key Location/Qualifiers FT source 1..2946 FT /organism="Cloning vector pBS-SK-Sfi" FT /mol_type="other DNA" FT /db_xref="taxon:512148" FT misc_feature 1..658 FT /note="derived from plasmid pBluescript SK(+), Genbank FT accession number X52328" FT misc_feature 653..658 FT /note="multiple cloning site; contains KpnI restriction FT site" FT misc_feature 659..707 FT /note="multiple cloning site; contains restriction sites FT for XhoI, SalI, SfiIB, EcoRI, PstI and SfiIA that were FT introduced during PCR amplification" FT misc_feature 708..2946 FT /note="derived from plasmid pBluescript SK(+), GenBank FT accession number X52328" FT misc_feature 708..745 FT /note="multiple cloning site; contains restriction sites FT for SpeI, XbaI, NotI, SacII, BstXI, and SacI" SQ Sequence 2946 BP; 742 A; 734 C; 752 G; 718 T; 0 other; ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccct 660 cgaggtcgac ggccgaggcg gccgaattct tctgcaggcc gtaatggcca ctagttctag 720 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc 780 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 840 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 900 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 960 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 1020 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 1080 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 1140 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 1200 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 1260 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 1320 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 1380 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 1440 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 1500 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 1560 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 1620 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 1680 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 1740 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1800 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 1860 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 1920 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 1980 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 2040 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 2100 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 2160 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 2220 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 2280 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 2340 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 2400 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 2460 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 2520 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 2580 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 2640 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 2700 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 2760 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 2820 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 2880 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 2940 tgccac 2946 pBS-SK-Sfi"Times New Roman Ys%͍PvuII(530),KpnI(658),XhoI(660),SalI(666),EagI(671),SfiI(679),EagI(680),EcoRI(685),PstI(697),SfiI(705),SpeI(711),XbaI(717),NotI(724),EagI(724) Arial / q"{!m Ys%SacI Arial- JrC|CC=I Ys%PvuII Arial Ybjj Ys%ScaI Arial  Ys%C f1_ori"Times New RomanYs%C !ORF 1"Times New RomanYs%C I_M13_pUC_fwd_primer"Times New RomanYs%C XhM13_forward20_primer"Times New Roman*Ys%CɆ r T7_promoter"Times New RomanYs%C  T3_promoter"Times New RomanYs% +M13_reverse_primer"Times New RomanYs%CɆ *@M13_pUC_rev_primer"Times New RomanYs%C Nk lac_promoter"Times New RomanYs%   Ampicillin"Times New RomanYs%  ORF 2"Times New Roman(Ys% , H  AmpR_promoter"Times New Roman Ys%test"Times New Roman rGWJ>\p\>