Sequence of pBS-SK-Sfi

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Sequence of pBS-SK-Sfi

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Sequence of pBS-SK-Sfi: 2946 BP; 742 A; 734 C; 752 G; 718 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60

1 gatttaacat tcgcaattat aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120

61 taaaaaattg gttatccggc tttagccgtt ttagggaata tttagttttc ttatctggct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180

121 ctatcccaac tcacaacaag gtcaaacctt gttctcaggt gataatttct tgcacctgag 180

.......... .......... .......... f1_ori .......... ..........

.......... .......... .......... .......... .......... ..........

181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240

181 gttgcagttt cccgcttttt ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg 240

.......... .......... f1_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300

241 gattagttca aaaaacccca gctccacggc atttcgtgat ttagccttgg gatttccctc 300

.......... ......... f1_ori ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360

301 gggggctaaa tctcgaactg cccctttcgg ccgcttgcac cgctctttcc ttcccttctt 360

.......... ......... f1_ori ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420

361 tcgctttcct cgcccgcgat cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg 420

.......... ......... f1_ori ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480

421 gtgtgggcgg cgcgaattac gcggcgatgt cccgcgcagg gtaagcggta agtccgacgc 480

.......... f1_ori ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540

481 gttgacaacc cttcccgcta gccacgcccg gagaagcgat aatgcggtcg accgctttcc 540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600

541 ccctacacga cgttccgcta attcaaccca ttgcggtccc aaaagggtca gtgctgcaac 600

.......... .......... ORF 1 .......... ...... M13_pUC_fwd_primer

.......... .......... .......... .......... .......... ..........

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccct 660

601 attttgctgc cggtcactcg cgcgcattat gctgagtgat atcccgctta acccatggga 660

M13_forward20_primer ORF 1 T7_promoter ........ ..........

SalI,EagI SfiI,EagI,EcoRI PstI SfiI705) SpeI(711)

661 cgaggtcgac ggccgaggcg gccgaattct tctgcaggcc gtaatggcca ctagttctag 720

661 gctccagctg ccggctccgc cggcttaaga agacgtccgg cattaccggt gatcaagatc 720

.......... .......... ORF 1 .......... .......... ..........

NotI(724),EagI(724) SacI(745) .......... .......... ..........

721 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc 780

721 tcgccggcgg tggcgccacc tcgaggtcga aaacaaggga aatcactccc aa ttaacgcg 780

.......... .......... ORF 1 ......... T3_promoter .........

.......... .......... .......... .......... .......... ..........

781 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 840

781 cgaaccgcat tagtaccagt atcgacaaag gacacacttt aacaataggc gagtgttaag 840

...... ORF 1 M13_reverse_primer .. M13_pUC_rev_primer ......

.......... .......... .......... .......... .......... ..........

841 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 900

841 gtgtgttgta tgctcggcct tcgtatttca catttcggac cccacggatt actcactcga 900

......... lac_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 960

901 ttgagtgtaa ttaacgcaac gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg 960

.......... .......... .......... .......... .......... ..........

PvuII(963) .......... .......... .......... .......... ..........

961 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 1020

961 tcgacgtaat tacttagccg gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 1080

1021 ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct cgccatagtc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 1140

1081 gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt cctttcttgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 1200

1141 acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 1260

1201 aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 1320

1261 ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 1380

1321 gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 1440

1381 accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 1500

1441 tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 1560

1501 tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 1620

1561 tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 1680

1621 tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc ggtcaatgga 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 1740

1681 agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1800

1741 aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 1860

1801 agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 1920

1861 actctaatag tttttcctag aagtggatct aggaaaattt aatttttact tcaaaattta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 1980

1921 gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat tagtcactcc 1980

.......... .......... .......... ....... Ampicillin ORF 2 .......

.......... .......... .......... .......... .......... ..........

1981 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 2040

1981 gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag gggcagcaca 2040

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 2100

2041 tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac tatggcgctc 2100

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 2160

2101 tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct tcccggctcg 2160

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 2220

2161 cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca acggcccttc 2220

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 2280

2221 gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa cgatgtccgt 2280

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 2340

2281 agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg gttgctagtt 2340

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 2400

2341 ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag ccaggaggct 2400

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 2460

2401 agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt cgtgacgtat 2460

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ScaI(2512)

2461 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 2520

2461 taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc atgagttggt 2520

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 2580

2521 tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc agttatgccc 2580

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 2640

2581 tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc 2640

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 2700

2641 ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt gggtgagcac 2700

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 2760

2701 gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact cgtttttgtc 2760

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 2820

2761 cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact tatgagtatg 2820

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 2880

2821 agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac tcgcctatgt 2880

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

2881 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 2940

2881 ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgccac.... .......... .......... .......... .......... .......... 2946

2941 acggtg.... .......... .......... .......... .......... .......... 2946

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-2946 Reference author: Sreenivasan R., Cai M., Bartfai R., Wang X., Christoffels A., Orban L.; Reference title: "Transcriptomic analysis reveal novel genes with sexually dimorphic Reference title: expression in the zebrafish gonad and brain"; Reference location: PLoS One 3(1791):1-16(2008).
Reference number: [2] Reference positions: 1-2946 Reference author: Sreenivasan R., Cai M., Bartfai R., Wang X., Christoffels A., Orban L.; Reference title: ; Reference location: Submitted (19-MAR-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Reproductive Genomics Laboratory, Temasek LifeSciences Laboratory, Reference location: Singapore 117604, Singapore