NtNt6ID EU626135; SV 1; circular; other DNA; STD; SYN; 6978 BP. AC EU626135; DT 24-AUG-2008 (Rel. 96, Created) DT 24-AUG-2008 (Rel. 96, Last updated, Version 1) DE Vector pBT20-Dbla-Tel-FRT, complete sequence. KW . OS Vector pBT20-Dbla-Tel-FRT OC other sequences; artificial sequences; vectors. RN [1] RP 1-6978 RA Kang Y., Barrett A.R., Son M.S.; RT "Genetic systems for single copy analysis based on non-antibiotic RT selectable marker in Burkholderia spp."; RL Unpublished. RN [2] RP 1-6978 RA Kang Y., Barrett A.R., Son M.S.; RT ; RL Submitted (06-APR-2008) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Hawaii at Manoa, 2538 McCarthy Mall Snyder Hall RL Rm 310, Honolulu, HI 96822, USA FH Key Location/Qualifiers FT source 1..6978 FT /organism="Vector pBT20-Dbla-Tel-FRT" FT /mol_type="other DNA" FT /db_xref="taxon:554285" FT gene 171..1217 FT /gene="tnp" FT CDS 171..1217 FT /codon_start=1 FT /transl_table=11 FT /gene="tnp" FT /product="Tnp" FT /note="transposase" FT /protein_id="ACH42099.1" FT /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY FT AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH FT IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH FT HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD FT YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP FT DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI FT ALEGNYVE" FT repeat_region 1456..1483 FT /note="pBT20-based mariner transposon repeat" FT promoter 1613..1654 FT /note="Burkholderia cenocepacia rpsL promoter; PCS12" FT gene 1694..2467 FT /gene="kilA" FT CDS 1694..2467 FT /codon_start=1 FT /transl_table=11 FT /gene="kilA" FT /product="KilA" FT /note="confers tellurite resistance" FT /protein_id="ACH42100.1" FT /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL FT MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL FT LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF FT DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA FT LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ" FT gene 2485..3621 FT /gene="telA" FT CDS 2485..3621 FT /codon_start=1 FT /transl_table=11 FT /gene="telA" FT /product="TelA" FT /note="confers tellurite resistance" FT /protein_id="ACH42101.1" FT /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE FT VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN FT RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA FT VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD FT LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL FT ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ FT EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV" FT gene 3618..4571 FT /gene="telB" FT CDS 3618..4571 FT /codon_start=1 FT /transl_table=11 FT /gene="telB" FT /product="TelB" FT /note="confers tellurite resistance" FT /protein_id="ACH42102.1" FT /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV FT TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF FT TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP FT SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG FT SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG FT AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP" FT terminator 4589..4620 FT /note="TonB terminator" FT repeat_region 4766..4789 FT /note="pBT20-based mariner transposon repeat" FT rep_origin 4940..5211 FT /note="R6K-gamma origin of replication" FT oriT 6240..6481 FT /note="origin of transfer for conjugation" SQ Sequence 6978 BP; 1586 A; 1918 C; 2047 G; 1427 T; 0 other; ccgccagtgt gatggattga cacatagatg gcgtcgctag tattaaatgc atattatttt 60 tatatagtac caaccttcaa atgattcgtg tcaaaatttg acgtctgtaa gtcaattagt 120 ttgtgagata gagcgtcttt tgtgaagcaa cttttgttat tgtgaaacat atggaaaaaa 180 aggaatttcg tgttttgata aaatactgtt ttctgaaggg aaaaaataca gtggaagcaa 240 aaacttggct tgataatgag tttccggact ctgccccagg gaaatcaaca ataattgatt 300 ggtatgcaaa attcaagcgt ggtgaaatga gcacggagga cggtgaacgc agtggacgcc 360 cgaaagaggt ggttaccgac gaaaacatca aaaaaatcca caaaatgatt ttgaatgacc 420 gtaaaatgaa gttgatcgag atagcagagg ccttaaagat atcaaaggaa cgtgttggtc 480 atatcattca tcaatatttg gatatgcgga agctctgtgc gaaatgggtg ccgcgcgagc 540 tcacatttga ccaaaaacaa cgacgtgttg atgattctaa gcggtgtttg cagctgttaa 600 ctcgtaatac acccgagttt ttccgtcgat atgtgacaat ggatgaaaca tggctccatc 660 actacactcc tgagtccaat cgacagtcgg ctgagtggac agcgaccggt gaaccgtctc 720 cgaagcgtgg aaagactcaa aagtccgctg gcaaagtaat ggcctctgtt ttttgggatg 780 cgcatggaat aatttttatc gattatcttg agaagggaaa aaccatcaac agtgactatt 840 atatggcgtt attggagcgt ttgaaggtcg aaatcgcggc aaaacggccc cacatgaaga 900 agaaaaaagt gttgttccac caagacaacg caccgtgcca caagtcattg agaacgatgg 960 caaaaattca tgaattgggc ttcgaattgc ttccccaccc accgtattct ccagatctgg 1020 cccccagcga ctttttcttg ttctcagacc tcaaaaggat gctcgcaggg aaaaaatttg 1080 gctgcaatga agaggtgatc gccgaaactg aggcctattt tgaggcaaaa ccgaaggagt 1140 actaccaaaa tggtatcaaa aaattggaag gtcgttataa tcgttgtatc gctcttgaag 1200 ggaactatgt tgaataataa aaacgaattt tgacaaaaaa atgtgttttt ctttgttaga 1260 ccggaatcct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 1320 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 1380 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccggtgccct gtacaagaaa 1440 gctgggtccc gggagtaaca ggttggctga taagtccccg gtctctagaa gggtaccgag 1500 ctcgaattag cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg 1560 gaataggaac ttcaagatcc cctgattccc tttgtcaaca gcaatggata tcagccgttg 1620 acttagttgg tatttccgga atatcatgct gggttccgaa taattttgtt taactttaag 1680 aaggagatat accatggaag aacaaagcgt gaacatggcg cgattgaagg gggaggtttt 1740 gcccgccctc ttcgcgtcgc cggcgacgat tggcgagtac ggggccggca tcgacggggc 1800 ggattccctc aacgagctgt cgaatctgat ggagcacggc gcagttgccg cgctggccga 1860 caaaatcagc cagatcgtgg cgaagctggc cgacgcggac ccccgcaaga tcgcggaaaa 1920 gcctacctgg ttcgagaaga tgcttggccg tgaggttgaa cgccaggtga ggtatcaggt 1980 cgcccgcaag acgctcgacc agttgctgga cgaagccgag ggcgtagcgc agcgcgtgcg 2040 ggacacgttg cgcgccttgg atgacatgct caatacgcat gaggccgagg tagaccggct 2100 cagagcctac attcaagccg ggcgcgagtt cctggacgag aaccccgagg ccggcgcggc 2160 caaggccggc gtgatcgagt tcgacaagcc gcgcgaacgc ttcgcgcgca agctcgccaa 2220 cctggcaacc ctcatggcgt cccatgaaat gagcgtcact cagatgaagc tcacgcgggc 2280 gcaggccgtg gacatgctgg accgcttctc tgaaacggca tccgtcctgg tgcccgtctg 2340 gcgtcagcac accctcgcgc tcatcaccac caagaacatg aatccggcaa tggtcgccga 2400 ggcggccaaa gctcaccagg cgctcatgcg gagcctttcg cagagcctgg aaggcatcaa 2460 ccaataacac ggcgggagaa ccctatgaac gcactgaaaa cgacgcacga cgccaaggcc 2520 cctatcgtcg ccttcgacat gaccccggca accctgcgcg agctgggctt gcaggaaagc 2580 gacgtgccgg aagtccatgc ggtcgcgcag cggatcgagg tcggcagtcc gcagaccgtt 2640 gccgagttcg gccgcgacgt ggccgagcac acgtcccgct acgccgatag cctgctggac 2700 caggtgcgca acagcgacct ggacgaagca ggcgagaaac tgacccaggt tgtcgccaag 2760 gcccgttccc tgaacgtcgg ccctttgtcc gacaaccgtt cccgcctgcc cctgattggc 2820 ccgctgatcg accgcttccg cgtccgttcg acgggcttca tggcgcgctt cgacacgacc 2880 cgcgagcaga tcgaacacct ggtcagcgaa gtgcagacca cccagcaagg catcgcgcag 2940 cgcaatgcct cgctcgacga aatgttcgca gccgtgcgcg aggaacaccg ccttcttggc 3000 gtccacatcg cggccggcaa ggtccgcctt gccgagctgc gcgagcaggc cgagggtctg 3060 cgcggcaatg tcgggaacga ccctggccgc gtgcaggagc tggccgacct cgatgcgatg 3120 gttgccaacc tggacaagcg catcggcgac ctgatcgcct tgcaacattc ggccatgcag 3180 agcctgccga ccatccgcat gatccaggcc aacaaccaga tgctggtcga taaattccac 3240 accatccgcg aaatcaccgt gccggcgtgg aagcggcaat tcatgctggc cttgagcctc 3300 aacgagcaga agaacgccgt cgaactggcc acggccatcg acgacaccac caacgacctg 3360 atgaagcgca atgcggccct gctgcatcgc acgtccgtcg agacggcgaa ggagaaccaa 3420 cgcctggtga tcgacgtgga cacgctcaag caggttcaga cgacgctcat caagaccgtc 3480 gaggacgtta ttcgcatcca gcaggaaggc gtgcagaagc gcaaggatgc cgagaagcag 3540 atcgccgcaa tgcgtggcga tcttcaagcc aagctgaccc gccagcccgt gcgcgagctg 3600 gcccaacagg agtccgtatg aatgccacaa acaccgatgt tttcgcccag gtaggcggcc 3660 tggaggcccg aggcgcgaag atgaagaagc ggggcacccg cttcctcatc gcggcgctgg 3720 cagtccttgc cattgccggg atcggggcag taacgggatg ggcgatcagc ccgagcgcga 3780 cgcccggaag cattgacgtg ccgcaggtgc tggcatcgac attcagcgac caggtgccgg 3840 gcagtgaggg cggcggcctg ggtggcggcc tgcccttcac ttcggccgtc ggggcattca 3900 cggacttcat ggcggggccg gcaattttta ccttgggcat tcttggcata gtggtcgcgg 3960 gtgccgtgct cgtgttcggg ggtgagttct gcgggttcgt gcgatccgtc tgcatgatgg 4020 tgatagccgt cagcatgatt ttcgtgtcgt cgaacttggt gaagggcatt ctcggcggcg 4080 atcacgacgc cggccctgcg gagccttcgc cgcgtgcgcg attcatggcg gccgtggagg 4140 ccaaggattt cgcgcgagtg caagagctga tcgaggcgcg tggagccaag tcggcggctg 4200 attatgtcct tgcgcagctc gccgtggccg aaggtctgga ccgcaagcct ggtgcgcgcg 4260 tcgtggtcgg gaaagcggcg ggcagcatgg caatgccgcc tgcggcgctg ggttttacgc 4320 caaggggaga agcggcatac gccatcgagc ggtcagccta tggtgagccg aggtccagca 4380 ttgcgaagca gtaccagcag gaatggaacc ggaaggcggc gacctggtgg gcgatggccg 4440 gtgtggccgg catcatcggc gcgatcctgg cggcggcggc aaccggcttt gttgggctgg 4500 cagtgtcgat ccgcaaccga gtgaagcgcg tgcgcgacct gttggtgatg gagccgggtg 4560 cagagccata agggatcgat ccccctcaag tcaaaagcct ccggtcggag gcttttgact 4620 ttctgctcga gctaattcca ttgagtaagt ttttaagcac atcagcttca aaagcgctct 4680 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa gatccccaat 4740 tcgagctcgg taccccacta gagaccgggg acttatcagc caacctgtta ctcccgggac 4800 cctgctttct tgtacagggc acaattccca tgtcagccgt taagtgttcc tgtgtcactc 4860 aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg cttgttgtcc 4920 acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt cttataaaac 4980 ttaaaacctt agaagctatt taagttgctg atttatatta attttattgg tcaaacatga 5040 aagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt agtacgttag 5100 ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga gagcttagta 5160 cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat cttcagatcc 5220 tctacgccgg acgcatcgtg gggatccagc cgaccaggct ttccacgccc gcgtgccgct 5280 ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt ttcgtgattg tcacgctcaa 5340 gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag ggcgcggtag gcccgatacg 5400 gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat ggcgatatgg atgtgcaggt 5460 tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc ggcgaagcca agcccagcgc 5520 agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg cttctctccc gcgcggaagc 5580 taaccagcag gtgataggtc ttgtcggcct cggaacgggt gttgccgtgc tgggtcgcca 5640 tcacctcggc catgacagcg ggcagggtgt ttgcctcgca gttcgtgacg cgcacgtgac 5700 ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc 5760 tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt 5820 agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca 5880 agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac 5940 cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg 6000 tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg 6060 tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt 6120 tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc 6180 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc 6240 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg 6300 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt 6360 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa 6420 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa 6480 aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg caaatgcagg 6540 aaattactga actgagggga caggcgagag acgatgccaa agagctacac cgacgagctg 6600 gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc tgcggttgcg 6660 ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt ccggctatgc gctcgtcacc 6720 atttgggagc acatgcggga aacggggaag gtcaagttct cctacgagac gttccgctcg 6780 cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc ccgcaccgca ggccaaggct 6840 gcggaacccg cgccggcacc caagacgccg gagccacggc ggccgaagca ggggggcaag 6900 gctgaaaagc cggcccccgc tgcggccccg accggcttca ccttcaaccc aacaccggac 6960 aaaaaggatc cactaggg 6978 BpBT20-Dbla-Tel-FRT"Times New Roman Nt3NdeI(170),EcoRV(461),SacI(542),PvuII(594),HpaI(599) ArialBg^OLO Nt ClaI(800),BglII(1014),ScaI(1141) ArialBCKRRu NtdPvuII(1395),XmaI(1449),SmaI(1451),XbaI(1485),KpnI(1497),SacI(1503),XbaI(1542),EcoRV(1610),NcoI(1693) Arial ~Bnw1 NtSexAI(1926),SfiI(2157) ArialBqW{YZNf Nt-EagI(2650),SexAI(2700),SexAI(2898),EagI(3012) Arial B$+11m  Nt,SexAI(3830),EagI(3884),EagI(4130),SfiI(4138) Arial,B? ;))6 NtcSexAI(4423),SfiI(4444),ClaI(4577),XhoI(4627),XbaI(4697),SacI(4748),KpnI(4754),XmaI(4794),SmaI(4796) ArialYBur Nt'HindIII(4940),HindIII(5042),BamHI(5243) ArialB[]^WLp NtEagI(5812),EagI(6105) ArialB} NtEagI(6881),BamHI(6967) ArialBuOtEi;)#i;NtCBtnp"Times New RomanNtB T7_promoter"Times New RomanNtCB,M13_forward20_primer"Times New RomanNtCɆB%;M13_pUC_fwd_primer"Times New RomanNtCB%FRT"Times New RomanNtCɆBT7_transl_en_RBS"Times New RomanNtCɆBORF 5"Times New RomanNtCB kilA"Times New RomanNtCBm ; ORF 1"Times New RomanNtCɆB %telA"Times New RomanNtBw ORF 2"Times New Roman*NtCB ORF 4"Times New RomanNtCB"telB"Times New Roman*NtBIxFRT"Times New RomanNtCBCUpUni_fwd_primer"Times New RomanNtCBfORF 3"Times New RomanNtCBoriT_RP4"Times New Roman Nttest"Times New RomanBlS7Y7