Sequence of pBT20-Dbla-Tel-FRT

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pBT20-Dbla-Tel-FRT

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pBT20-Dbla-Tel-FRT: 6978 BP; 1586 A; 1918 C; 2047 G; 1427 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ccgccagtgt gatggattga cacatagatg gcgtcgctag tattaaatgc atattatttt 60

1 ggcggtcaca ctacctaact gtgtatctac cgcagcgatc ataatttacg tataataaaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tatatagtac caaccttcaa atgattcgtg tcaaaatttg acgtctgtaa gtcaattagt 120

61 atatatcatg gttggaagtt tactaagcac agttttaaac tgcagacatt cagttaatca 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttgtgagata gagcgtcttt tgtgaagcaa cttttgttat tgtgaaacat atggaaaaaa 180

121 aacactctat ctcgcagaaa acacttcgtt gaaaacaata acactttgta tacctttttt 180

.......... .......... .......... .......... .......... tnp

.......... .......... .......... .......... .......... ..........

181 aggaatttcg tgttttgata aaatactgtt ttctgaaggg aaaaaataca gtggaagcaa 240

181 tccttaaagc acaaaactat tttatgacaa aagacttccc ttttttatgt caccttcgtt 240

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aaacttggct tgataatgag tttccggact ctgccccagg gaaatcaaca ataattgatt 300

241 tttgaaccga actattactc aaaggcctga gacggggtcc ctttagttgt tattaactaa 300

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggtatgcaaa attcaagcgt ggtgaaatga gcacggagga cggtgaacgc agtggacgcc 360

301 ccatacgttt taagttcgca ccactttact cgtgcctcct gccacttgcg tcacctgcgg 360

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cgaaagaggt ggttaccgac gaaaacatca aaaaaatcca caaaatgatt ttgaatgacc 420

361 gctttctcca ccaatggctg cttttgtagt ttttttaggt gttttactaa aacttactgg 420

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtaaaatgaa gttgatcgag atagcagagg ccttaaagat atcaaaggaa cgtgttggtc 480

421 cattttactt caactagctc tatcgtctcc ggaatttcta tagtttcctt gcacaaccag 480

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atatcattca tcaatatttg gatatgcgga agctctgtgc gaaatgggtg ccgcgcgagc 540

481 tatagtaagt agttataaac ctatacgcct tcgagacacg ctttacccac ggcgcgctcg 540

.......... .......... tnp .......... .......... ..........

SacI(542) .......... .......... .......... ..... PvuII(594),HpaI(599)

541 tcacatttga ccaaaaacaa cgacgtgttg atgattctaa gcggtgtttg cagctgttaa 600

541 agtgtaaact ggtttttgtt gctgcacaac tactaagatt cgccacaaac gtcgacaatt 600

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ctcgtaatac acccgagttt ttccgtcgat atgtgacaat ggatgaaaca tggctccatc 660

601 gagcattatg tgggctcaaa aaggcagcta tacactgtta cctactttgt accgaggtag 660

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actacactcc tgagtccaat cgacagtcgg ctgagtggac agcgaccggt gaaccgtctc 720

661 tgatgtgagg actcaggtta gctgtcagcc gactcacctg tcgctggcca cttggcagag 720

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgaagcgtgg aaagactcaa aagtccgctg gcaaagtaat ggcctctgtt ttttgggatg 780

721 gcttcgcacc tttctgagtt ttcaggcgac cgtttcatta ccggagacaa aaaaccctac 780

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgcatggaat aatttttatc gattatcttg agaagggaaa aaccatcaac agtgactatt 840

781 gcgtacctta ttaaaaatag ctaatagaac tcttcccttt ttggtagttg tcactgataa 840

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atatggcgtt attggagcgt ttgaaggtcg aaatcgcggc aaaacggccc cacatgaaga 900

841 tataccgcaa taacctcgca aacttccagc tttagcgccg ttttgccggg gtgtacttct 900

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agaaaaaagt gttgttccac caagacaacg caccgtgcca caagtcattg agaacgatgg 960

901 tcttttttca caacaaggtg gttctgttgc gtggcacggt gttcagtaac tcttgctacc 960

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 caaaaattca tgaattgggc ttcgaattgc ttccccaccc accgtattct ccagatctgg 1020

961 gtttttaagt acttaacccg aagcttaacg aaggggtggg tggcataaga ggtctagacc 1020

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cccccagcga ctttttcttg ttctcagacc tcaaaaggat gctcgcaggg aaaaaatttg 1080

1021 gggggtcgct gaaaaagaac aagagtctgg agttttccta cgagcgtccc ttttttaaac 1080

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gctgcaatga agaggtgatc gccgaaactg aggcctattt tgaggcaaaa ccgaaggagt 1140

1081 cgacgttact tctccactag cggctttgac tccggataaa actccgtttt ggcttcctca 1140

.......... .......... tnp .......... .......... ..........

ScaI(1141) .......... .......... .......... .......... ..........

1141 actaccaaaa tggtatcaaa aaattggaag gtcgttataa tcgttgtatc gctcttgaag 1200

1141 tgatggtttt accatagttt tttaaccttc cagcaatatt agcaacatag cgagaacttc 1200

.......... .......... tnp .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggaactatgt tgaataataa aaacgaattt tgacaaaaaa atgtgttttt ctttgttaga 1260

1201 ccttgataca acttattatt tttgcttaaa actgtttttt tacacaaaaa gaaacaatct 1260

tnp .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccggaatcct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 1320

1261 ggccttagga ggttaagcgg gatatcactc agcataatgc gcgcgagtga ccggcagcaa 1320

.......... ......... T7_promoter ......... ..... M13_forward20_primer

.......... .......... .......... .......... .......... ..........

1321 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 1380

1321 aatgttgcag cactgaccct tttgggaccg caatgggttg aattagcgga acgtcgtgta 1380

M13_pUC_fwd_primer .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccggtgccct gtacaagaaa 1440

1381 gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt ggccacggga catgttcttt 1440

.......... .......... .......... .......... .......... ..........

.... XmaI(1449),SmaI(1451) .... .......... XbaI(1485) ..........

1441 gctgggtccc gggagtaaca ggttggctga taagtccccg gtctctagaa gggtaccgag 1500

1441 cgacccaggg ccctcattgt ccaaccgact attcaggggc cagagatctt cccatggctc 1500

.......... .......... .......... .......... .......... ..........

SacI(1503) .......... .......... .......... .......... ..........

1501 ctcgaattag cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg 1560

1501 gagcttaatc gaagttttcg cgagacttca aggatatgaa agatctctta tccttgaagc 1560

.......... .......... .......... .......... FRT ..........

.......... .......... .......... .......... .......... ..........

1561 gaataggaac ttcaagatcc cctgattccc tttgtcaaca gcaatggata tcagccgttg 1620

1561 cttatccttg aagttctagg ggactaaggg aaacagttgt cgttacctat agtcggcaac 1620

FRT .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acttagttgg tatttccgga atatcatgct gggttccgaa taattttgtt taactttaag 1680

1621 tgaatcaacc ataaaggcct tatagtacga cccaaggctt attaaaacaa attgaaattc 1680

.......... .......... .......... .......... ....... T7_transl_en_RBS

.......... NcoI(1693) .......... .......... .......... ..........

1681 aaggagatat accatggaag aacaaagcgt gaacatggcg cgattgaagg gggaggtttt 1740

1681 ttcctctata tggtaccttc ttgtttcgca cttgtaccgc gctaacttcc ccctccaaaa 1740

.......... .......... ORF 5 kilA .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcccgccctc ttcgcgtcgc cggcgacgat tggcgagtac ggggccggca tcgacggggc 1800

1741 cgggcgggag aagcgcagcg gccgctgcta accgctcatg ccccggccgt agctgccccg 1800

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggattccctc aacgagctgt cgaatctgat ggagcacggc gcagttgccg cgctggccga 1860

1801 cctaagggag ttgctcgaca gcttagacta cctcgtgccg cgtcaacggc gcgaccggct 1860

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caaaatcagc cagatcgtgg cgaagctggc cgacgcggac ccccgcaaga tcgcggaaaa 1920

1861 gttttagtcg gtctagcacc gcttcgaccg gctgcgcctg ggggcgttct agcgcctttt 1920

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gcctacctgg ttcgagaaga tgcttggccg tgaggttgaa cgccaggtga ggtatcaggt 1980

1921 cggatggacc aagctcttct acgaaccggc actccaactt gcggtccact ccatagtcca 1980

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgcccgcaag acgctcgacc agttgctgga cgaagccgag ggcgtagcgc agcgcgtgcg 2040

1981 gcgggcgttc tgcgagctgg tcaacgacct gcttcggctc ccgcatcgcg tcgcgcacgc 2040

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggacacgttg cgcgccttgg atgacatgct caatacgcat gaggccgagg tagaccggct 2100

2041 cctgtgcaac gcgcggaacc tactgtacga gttatgcgta ctccggctcc atctggccga 2100

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... SfiI(2157)

2101 cagagcctac attcaagccg ggcgcgagtt cctggacgag aaccccgagg ccggcgcggc 2160

2101 gtctcggatg taagttcggc ccgcgctcaa ggacctgctc ttggggctcc ggccgcgccg 2160

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caaggccggc gtgatcgagt tcgacaagcc gcgcgaacgc ttcgcgcgca agctcgccaa 2220

2161 gttccggccg cactagctca agctgttcgg cgcgcttgcg aagcgcgcgt tcgagcggtt 2220

.......... .......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cctggcaacc ctcatggcgt cccatgaaat gagcgtcact cagatgaagc tcacgcgggc 2280

2221 ggaccgttgg gagtaccgca gggtacttta ctcgcagtga gtctacttcg agtgcgcccg 2280

.......... ORF 5 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcaggccgtg gacatgctgg accgcttctc tgaaacggca tccgtcctgg tgcccgtctg 2340

2281 cgtccggcac ctgtacgacc tggcgaagag actttgccgt aggcaggacc acgggcagac 2340

.......... .......... kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcgtcagcac accctcgcgc tcatcaccac caagaacatg aatccggcaa tggtcgccga 2400

2341 cgcagtcgtg tgggagcgcg agtagtggtg gttcttgtac ttaggccgtt accagcggct 2400

.......... .......... kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcggccaaa gctcaccagg cgctcatgcg gagcctttcg cagagcctgg aaggcatcaa 2460

2401 ccgccggttt cgagtggtcc gcgagtacgc ctcggaaagc gtctcggacc ttccgtagtt 2460

.......... .......... kilA ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccaataacac ggcgggagaa ccctatgaac gcactgaaaa cgacgcacga cgccaaggcc 2520

2461 ggttattgtg ccgccctctt gggatacttg cgtgactttt gctgcgtgct gcggttccgg 2520

.......... .......... ORF 1 .......... telA ..........

.......... .......... .......... .......... .......... ..........

2521 cctatcgtcg ccttcgacat gaccccggca accctgcgcg agctgggctt gcaggaaagc 2580

2521 ggatagcagc ggaagctgta ctggggccgt tgggacgcgc tcgacccgaa cgtcctttcg 2580

.......... .......... ORF 1 telA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gacgtgccgg aagtccatgc ggtcgcgcag cggatcgagg tcggcagtcc gcagaccgtt 2640

2581 ctgcacggcc ttcaggtacg ccagcgcgtc gcctagctcc agccgtcagg cgtctggcaa 2640

.......... .......... ORF 1 telA .......... .......... ..........

EagI(2650) .......... .......... .......... .......... ..........

2641 gccgagttcg gccgcgacgt ggccgagcac acgtcccgct acgccgatag cctgctggac 2700

2641 cggctcaagc cggcgctgca ccggctcgtg tgcagggcga tgcggctatc g gacgacctg 2700

.......... .......... ORF 1 telA .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

2701 caggtgcgca acagcgacct ggacgaagca ggcgagaaac tgacccaggt tgtcgccaag 2760

2701 gtccacgcgt tgtcgctgga cctgcttcgt ccgctctttg actgggtcca acagcggttc 2760

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcccgttccc tgaacgtcgg ccctttgtcc gacaaccgtt cccgcctgcc cctgattggc 2820

2761 cgggcaaggg acttgcagcc gggaaacagg ctgttggcaa gggcggacgg ggactaaccg 2820

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccgctgatcg accgcttccg cgtccgttcg acgggcttca tggcgcgctt cgacacgacc 2880

2821 ggcgactagc tggcgaaggc gcaggcaagc tgcccgaagt accgcgcgaa gctgtgctgg 2880

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgcgagcaga tcgaacacct ggtcagcgaa gtgcagacca cccagcaagg catcgcgcag 2940

2881 gcgctcgtct agcttgtgga ccagtcgctt cacgtctggt gggtcgttcc gtagcgcgtc 2940

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgcaatgcct cgctcgacga aatgttcgca gccgtgcgcg aggaacaccg ccttcttggc 3000

2941 gcgttacgga gcgagctgct ttacaagcgt cggcacgcgc tccttgtggc ggaagaaccg 3000

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... EagI(3012) .......... .......... .......... ..........

3001 gtccacatcg cggccggcaa ggtccgcctt gccgagctgc gcgagcaggc cgagggtctg 3060

3001 caggtgtagc gccggccgtt ccaggcggaa cggctcgacg cgctcgtccg gctcccagac 3060

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgcggcaatg tcgggaacga ccctggccgc gtgcaggagc tggccgacct cgatgcgatg 3120

3061 gcgccgttac agcccttgct gggaccggcg cacgtcctcg accggctgga gctacgctac 3120

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gttgccaacc tggacaagcg catcggcgac ctgatcgcct tgcaacattc ggccatgcag 3180

3121 caacggttgg acctgttcgc gtagccgctg gactagcgga acgttgtaag ccggtacgtc 3180

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agcctgccga ccatccgcat gatccaggcc aacaaccaga tgctggtcga taaattccac 3240

3181 tcggacggct ggtaggcgta ctaggtccgg ttgttggtct acgaccagct atttaaggtg 3240

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 accatccgcg aaatcaccgt gccggcgtgg aagcggcaat tcatgctggc cttgagcctc 3300

3241 tggtaggcgc tttagtggca cggccgcacc ttcgccgtta agtacgaccg gaactcggag 3300

.......... ....... ORF 1 telA ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aacgagcaga agaacgccgt cgaactggcc acggccatcg acgacaccac caacgacctg 3360

3301 ttgctcgtct tcttgcggca gcttgaccgg tgccggtagc tgctgtggtg gttgctggac 3360

.......... ORF 2 ORF 1 telA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atgaagcgca atgcggccct gctgcatcgc acgtccgtcg agacggcgaa ggagaaccaa 3420

3361 tacttcgcgt tacgccggga cgacgtagcg tgcaggcagc tctgccgctt cctcttggtt 3420

.......... ORF 1 telA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgcctggtga tcgacgtgga cacgctcaag caggttcaga cgacgctcat caagaccgtc 3480

3421 gcggaccact agctgcacct gtgcgagttc gtccaagtct gctgcgagta gttctggcag 3480

.......... .......... telA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gaggacgtta ttcgcatcca gcaggaaggc gtgcagaagc gcaaggatgc cgagaagcag 3540

3481 ctcctgcaat aagcgtaggt cgtccttccg cacgtcttcg cgttcctacg gctcttcgtc 3540

.......... .......... telA ORF 4 .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atcgccgcaa tgcgtggcga tcttcaagcc aagctgaccc gccagcccgt gcgcgagctg 3600

3541 tagcggcgtt acgcaccgct agaagttcgg ttcgactggg cggtcgggca cgcgctcgac 3600

.......... .......... telA ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcccaacagg agtccgtatg aatgccacaa acaccgatgt tttcgcccag gtaggcggcc 3660

3601 cgggttgtcc tcaggcatac ttacggtgtt tgtggctaca aaagcgggtc catccgccgg 3660

.......... telA ORF 4 telB .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tggaggcccg aggcgcgaag atgaagaagc ggggcacccg cttcctcatc gcggcgctgg 3720

3661 acctccgggc tccgcgcttc tacttcttcg ccccgtgggc gaaggagtag cgccgcgacc 3720

.......... .......... ORF 4 telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cagtccttgc cattgccggg atcggggcag taacgggatg ggcgatcagc ccgagcgcga 3780

3721 gtcaggaacg gtaacggccc tagccccgtc attgccctac ccgctagtcg ggctcgcgct 3780

.......... .......... ORF 4 telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgcccggaag cattgacgtg ccgcaggtgc tggcatcgac attcagcgac caggtgccgg 3840

3781 gcgggccttc gtaactgcac ggcgtccacg accgtagctg taagtcgctg gtccacggcc 3840

.......... .......... ORF 4 telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcagtgaggg cggcggcctg ggtggcggcc tgcccttcac ttcggccgtc ggggcattca 3900

3841 cgtcactccc gccgccggac ccaccgccgg acgggaagtg aagccggcag ccccgtaagt 3900

.......... .......... ORF 4 telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cggacttcat ggcggggccg gcaattttta ccttgggcat tcttggcata gtggtcgcgg 3960

3901 gcctgaagta ccgccccggc cgttaaaaat ggaacccgta agaaccgtat caccagcgcc 3960

.......... .......... ORF 4 telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtgccgtgct cgtgttcggg ggtgagttct gcgggttcgt gcgatccgtc tgcatgatgg 4020

3961 cacggcacga gcacaagccc ccactcaaga cgcccaagca cgctaggcag acgtactacc 4020

.......... .......... ORF 4 telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgatagccgt cagcatgatt ttcgtgtcgt cgaacttggt gaagggcatt ctcggcggcg 4080

4021 actatcggca gtcgtactaa aagcacagca gcttgaacca cttcccgtaa gagccgccgc 4080

.......... .......... ORF 4 telB .......... .......... ..........

.......... .......... .......... .......... EagI(4130) SfiI(4138)

4081 atcacgacgc cggccctgcg gagccttcgc cgcgtgcgcg attcatggcg gccgtggagg 4140

4081 tagtgctgcg gccgggacgc ctcggaagcg gcgcacgcgc taagtaccgc cggcacctcc 4140

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccaaggattt cgcgcgagtg caagagctga tcgaggcgcg tggagccaag tcggcggctg 4200

4141 ggttcctaaa gcgcgctcac gttctcgact agctccgcgc acctcggttc agccgccgac 4200

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 attatgtcct tgcgcagctc gccgtggccg aaggtctgga ccgcaagcct ggtgcgcgcg 4260

4201 taatacagga acgcgtcgag cggcaccggc ttccagacct ggcgttcgga ccacgcgcgc 4260

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcgtggtcgg gaaagcggcg ggcagcatgg caatgccgcc tgcggcgctg ggttttacgc 4320

4261 agcaccagcc ctttcgccgc ccgtcgtacc gttacggcgg acgccgcgac ccaaaatgcg 4320

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 caaggggaga agcggcatac gccatcgagc ggtcagccta tggtgagccg aggtccagca 4380

4321 gttcccctct tcgccgtatg cggtagctcg ccagtcggat accactcggc tccaggtcgt 4380

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ttgcgaagca gtaccagcag gaatggaacc ggaaggcggc gacctggtgg gcgatggccg 4440

4381 aacgcttcgt catggtcgtc cttaccttgg ccttccgccg ctggaccacc cgctaccggc 4440

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtgtggccgg catcatcggc gcgatcctgg cggcggcggc aaccggcttt gttgggctgg 4500

4441 cacaccggcc gtagtagccg cgctaggacc gccgccgccg ttggccgaaa caacccgacc 4500

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cagtgtcgat ccgcaaccga gtgaagcgcg tgcgcgacct gttggtgatg gagccgggtg 4560

4501 gtcacagcta ggcgttggct cacttcgcgc acgcgctgga caaccactac ctcggcccac 4560

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cagagccata agggatcgat ccccctcaag tcaaaagcct ccggtcggag gcttttgact 4620

4561 gtctcggtat tccctagcta gggggagttc agttttcgga ggccagcctc cgaaaactga 4620

telB .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttctgctcga gctaattcca ttgagtaagt ttttaagcac atcagcttca aaagcgctct 4680

4621 aagacgagct cgattaaggt aactcattca aaaattcgtg tagtcgaagt tttcgcgaga 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa gatccccaat 4740

4681 cttcaaggat atgaaagatc tcttatcctt gaagccttat ccttgaagtt ctaggggtta 4740

.......... .......... FRT .......... .......... ..........

SacI(4748) KpnI(4754) .......... .......... .... XmaI(4794),SmaI(4796)

4741 tcgagctcgg taccccacta gagaccgggg acttatcagc caacctgtta ctcccgggac 4800

4741 agctcgagcc atggggtgat ctctggcccc tgaatagtcg gttggacaat gagggccctg 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cctgctttct tgtacagggc acaattccca tgtcagccgt taagtgttcc tgtgtcactc 4860

4801 ggacgaaaga acatgtcccg tgttaagggt acagtcggca attcacaagg acacagtgag 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg cttgttgtcc 4920

4861 ttttaacgaa actctccgag attcccgaag agtcacgcaa tgtagggacc gaacaacagg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt cttataaaac 4980

4921 tgttggcaat ttggaatttt cgaaattttc ggaatatata agaaaaaaaa gaatattttg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ttaaaacctt agaagctatt taagttgctg atttatatta attttattgg tcaaacatga 5040

4981 aattttggaa tcttcgataa attcaacgac taaatataat taaaataacc agtttgtact 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt agtacgttag 5100

5041 ttcgaatcat gcactttgta ctctcgaatc atgcaatcgg tactctcgaa tcatgcaatc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga gagcttagta 5160

5101 ggtactccca aatcaagcaa tttgtactct cgaatcatgc aatttgtact ctcgaatcat 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat cttcagatcc 5220

5161 gcactttgta ctctcgaatc atgcatgata gttgtccaac ttgacgacta gaagtctagg 5220

.......... .......... ....... pUni_fwd_primer ....... ..........

.......... ......... BamHI(5243) ......... .......... ..........

5221 tctacgccgg acgcatcgtg gggatccagc cgaccaggct ttccacgccc gcgtgccgct 5280

5221 agatgcggcc tgcgtagcac ccctaggtcg gctggtccga aaggtgcggg cgcacggcga 5280

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt ttcgtgattg tcacgctcaa 5340

5281 ggtacagcaa gcgcgccaag agcctttgcg cgacggcgca aagcactaac agtgcgagtt 5340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag ggcgcggtag gcccgatacg 5400

5341 cgggcatcag ggcaagctcg cagcgcgtct ccagtcgctc ccgcgccatc cgggctatgc 5400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat ggcgatatgg atgtgcaggt 5460

5401 cgagtaccta ccacaaagcc cagcccactt agaacaacta ccgctatacc tacacgtcca 5460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc ggcgaagcca agcccagcgc 5520

5461 acagccacag cactacgtgc cgtgactgcg cgactacgag ccgcttcggt tcgggtcgcg 5520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg cttctctccc gcgcggaagc 5580

5521 tctacgccag gagttagcgc gcgttgcaga ggcgcagccc gaagagaggg cgcgccttcg 5580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 taaccagcag gtgataggtc ttgtcggcct cggaacgggt gttgccgtgc tgggtcgcca 5640

5581 attggtcgtc cactatccag aacagccgga gccttgccca caacggcacg acccagcggt 5640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tcacctcggc catgacagcg ggcagggtgt ttgcctcgca gttcgtgacg cgcacgtgac 5700

5641 agtggagccg gtactgtcgc ccgtcccaca aacggagcgt caagcactgc gcgtgcactg 5700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc 5760

5701 ggtccgcgag ccagaacgga acgagcagcc actacatgaa gtggtcgagg cgcttcagcg 5760

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt 5820

5761 agaagaacta cctcgcgtac ccctgcacga accgttagtg cgcgtggggg gccggcaaaa 5820

ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca 5880

5821 tcgccgattt tttcagtacc gagacgggag cccgcctggt gcgggtagta ctggaacggt 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac 5940

5881 tcgagcagga cgaagagaag ctagaagcgg tcgtcccgct cctagcaccg tagtggcttg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg 6000

5941 gcgcggcacg cgcccagcag ccactcggtc tcaaagtcgt ccggcgggtc cgccgggtcc 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg 6060

6001 agcggtaact acgcccggtc gagcgcctgc acgagtatca ggtgctgcgg gcactaaaac 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... EagI(6105) ..........

6061 tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt 6120

6061 atcgggaccg gctgccggtc gtccatccgg ctgtccgagt acggccggcg gcggcggaaa 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc 6180

6121 aggagttagc gagaagcaag cagaccttcc gtcatgtgga actatccacc cgacgggaag 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc 6240

6181 gaccaaccga accaaagtag tcggtaggcg aacgggagta gacaatgcgg ccgccatcgg 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg 6300

6241 ccggtcggag cgtctcgtcc taagggcaac tcgtggcggt ccacgcttat tccctgtcac 6300

.......... .......... .......... .......... .......... oriT_RP4

.......... .......... .......... .......... .......... ..........

6301 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt 6360

6301 ttcttccttg tgggcgagcg cccacccgga tgaagtggat aggacgggcc gactgcggca 6360

.......... .......... oriT_RP4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa 6420

6361 acctatgtgg ttcctttcag atgtgcttgg gaaaccgttt taggacatat agcacgcttt 6420

.......... oriT_RP4 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa 6480

6421 ttcctaccta tatggctttt ttagcgatat tactggggct tcgtcccaat acgtcgcctt 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg caaatgcagg 6540

6481 ttcgcgacga agggacgaca aaacacctta tagatggctg acctttgtcc gtttacgtcc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aaattactga actgagggga caggcgagag acgatgccaa agagctacac cgacgagctg 6600

6541 tttaatgact tgactcccct gtccgctctc tgctacggtt tctcgatgtg gctgctcgac 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc tgcggttgcg 6660

6601 cggctcaccc aacttagggc gcgccggttc ttcgcggccg cactactccg acgccaacgc 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt ccggctatgc gctcgtcacc 6720

6661 aaggaccgcc actcccgcct acagctccgc cgcaatcgca ggccgatacg cgagcagtgg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 atttgggagc acatgcggga aacggggaag gtcaagttct cctacgagac gttccgctcg 6780

6721 taaaccctcg tgtacgccct ttgccccttc cagttcaaga ggatgctctg caaggcgagc 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc ccgcaccgca ggccaaggct 6840

6781 gtgcggtccg ccgtgtagtt ccggttcggg cggctacacg ggcgtggcgt ccggttccga 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gcggaacccg cgccggcacc caagacgccg gagccacggc ggccgaagca ggggggcaag 6900

6841 cgccttgggc gcggccgtgg gttctgcggc ctcggtgccg ccggcttcgt ccccccgttc 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gctgaaaagc cggcccccgc tgcggccccg accggcttca ccttcaaccc aacaccggac 6960

6901 cgacttttcg gccgggggcg acgccggggc tggccgaagt ggaagttggg ttgtggcctg 6960

.......... .......... .......... .......... .......... ..........

BamHI(6967) ........ .......... .......... .......... ..........

6961 aaaaaggatc cactaggg.. .......... .......... .......... .......... 6978

6961 tttttcctag gtgatccc.. .......... .......... .......... .......... 6978

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-6978 Reference author: Kang Y., Barrett A.R., Son M.S.; Reference title: "Genetic systems for single copy analysis based on non-antibiotic Reference title: selectable marker in Burkholderia spp."; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-6978 Reference author: Kang Y., Barrett A.R., Son M.S.; Reference title: ; Reference location: Submitted (06-APR-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Microbiology, University of Hawaii at Manoa, 2538 McCarthy Mall Snyder Hall Reference location: Rm 310, Honolulu, HI 96822, USA