Sequence of pBV-Luc

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Sequence of pBV-Luc

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Sequence of pBV-Luc : 4867 BP; 1264 A; 1155 C; 1173 G; 1275 T; 0 other;

KpnI(5) SacI(11) NheI(21) SmaI(28) PvuII(39) .. ..........

1 ggtaccgagc tcttacgcgt gctagcccgg gctcgacagc tggacgtcga tatcgaattc 60

1 ccatggctcg agaatgcgca cgatcgggcc cgagctgtcg acctgcagct atagcttaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... BglII(85) .......... .......... ..........

61 gggtatataa tggatccggt atcgagatct gcgatctaag taagcttggc attccggtac 120

61 cccatatatt acctaggcca tagctctaga cgctagattc attcgaaccg taaggccatg 120

.......... .......... .......... .......... .......... ..........

.......... NcoI(135) .......... .......... NarI(170) ..........

121 tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta 180

121 acaaccattt cggtggtacc ttctgcggtt tttgtatttc tttccgggcc gcggtaagat 180

.......... .......... ... ORF 2 firefly_Luciferase ... ..........

.......... .......... .......... .......... .......... ..........

181 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct 240

181 aggcgacctt ctaccttggc gacctctcgt tgacgtattc cgatacttct ctatgcggga 240

.......... .. ORF 2 firefly_Luciferase LucNrev_pri .. ..........

.......... .......... .......... .......... .......... ..........

241 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga 300

241 ccaaggacct tgttaacgaa aatgtctacg tgtatagctc cacctgtagt gaatgcgact 300

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

BstBI(306) .......... .......... .......... .......... ..........

301 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa 360

301 catgaagctt tacaggcaag ccaaccgtct tcgatacttt gctatacccg acttatgttt 360

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc 420

361 agtgtcttag cagcatacgt cacttttgag agaagttaag aaatacggcc acaacccgcg 420

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct 480

421 caataaatag cctcaacgtc aacgcgggcg cttgctgtaa atattacttg cacttaacga 480

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa 540

481 gttgtcatac ccgtaaagcg tcggatggca ccacaagcaa aggtttttcc ccaacgtttt 540

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa 600

541 ttaaaacttg cacgtttttt tcgagggtta gtaggttttt taataatagt acctaagatt 600

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg 660

601 ttgcctaatg gtccctaaag tcagctacat gtgcaagcag tgtagagtag atggagggcc 660

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... BclI(717)

661 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat 720

661 aaaattactt atgctaaaac acggtctcag gaagctatcc ctgttctgtt aacgtgacta 720

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

721 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc 780

721 gtacttgagg agacctagat gaccagacgg atttccacag cgagacggag tatcttgacg 780

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac 840

781 gacgcactct aagagcgtac ggtctctagg ataaaaaccg ttagtttagt aaggcctatg 840

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata 900

841 acgctaaaat tcacaacaag gtaaggtagt gccaaaacct tacaaatgat gtgagcctat 900

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag 960

901 aaactataca cctaaagctc agcagaatta catatctaaa cttcttctcg acaaagactc 960

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt 1020

961 ctcggaagtc ctaatgttct aagtttcacg cgacgaccac ggttgggata agaggaagaa 1020

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg 1080

1021 gcggttttcg tgagactaac tgtttatgct aaatagatta aatgtgcttt aacgaagacc 1080

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg 1140

1081 accgcgaggg gagagattcc ttcagcccct tcgccaacgg ttctccaagg tagacggtcc 1140

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg 1200

1141 atagtccgtt cctatacccg agtgactctg atgtagtcga taagactaat gtgggctccc 1200

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga 1260

1201 cctactattt ggcccgcgcc agccatttca acaaggtaaa aaacttcgct tccaacacct 1260

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc 1320

1261 agacctatgg cccttttgcg acccgcaatt agtttctccg cttgacacac actctccagg 1320

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga 1380

1321 atactaatac aggccaatac atttgttagg ccttcgctgg ttgcggaact aactgttcct 1380

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt 1440

1381 acctaccgat gtaagacctc tgtatcgaat gaccctgctt ctgcttgtga agaagtagca 1440

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga 1500

1441 actggcggac ttcagagact aattcatgtt tccgatagtc caccgagggc gacttaacct 1500

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga 1560

1501 taggtagaac gaggttgtgg ggttgtagaa gctgcgtcca cagcgtccag aagggctgct 1560

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga 1620

1561 actgcggcca cttgaagggc ggcggcaaca acaaaacctc gtgcctttct gctactgcct 1620

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg 1680

1621 ttttctctag cacctaatgc agcggtcagt tcattgttgg cgctttttca acgcgcctcc 1680

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat 1740

1681 tcaacacaaa cacctgcttc atggctttcc agaatggcct tttgagctgc gttcttttta 1740

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... XbaI(1791)

1741 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg 1800

1741 gtctctctag gagtatttcc ggttcttccc gcctttctag cggcacatta agatctcagc 1800

.......... ... ORF 2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac 1860

1801 cccgccggcc ggcgaagctc gtctgtacta ttctatgtaa ctactcaaac ctgtttggtg 1860

.......... .......... .......... ... SV40_PA_term EBV_rev_pri ...

.......... .......... .......... .......... .......... ..........

1861 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 1920

1861 ttgatcttac gtcacttttt ttacgaaata aacactttaa acactacgat aacgaaataa 1920

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 1980

1921 acattggtaa tattcgacgt tatttgttca attgttgttg ttaacgtaag taaaatacaa 1980

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 2040

1981 agtccaagtc cccctccaca ccctccaaaa aatttcgttc attttggaga tgtttacacc 2040

.......... .......... .......... .......... .......... ..........

.......... SalI(2059) .......... .......... .......... ..........

2041 taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 2100

2041 attttagcta ttcctaggca gctggctacg ggaactctcg gaagttgggt cagtcgagga 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 2160

2101 aggccacccg cgccccgtac tgatagcagc ggcgtgaata ctgacagaag aaatagtacg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc 2220

2161 ttgagcatcc tgtccacggc cgtcgcgaga aggcgaagga gcgagtgact gagcgacgcg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 2280

2221 agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2340

2281 tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2400

2341 ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta 2400

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2460

2401 gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2520

2461 cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg 2580

2521 atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagttacgag tgcgacatcc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2640

2581 atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2700

2641 gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2760

2701 ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 2820

2761 ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc ctgtcataaa 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2880

2821 ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2940

2881 ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 3000

2941 tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc 3000

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 3060

3001 ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta gaagtggatc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3120

3061 taggaaaatt taatttttac ttcaaaattt agttagattt catatatact catttgaacc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3180

3121 agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac agataaagca 3180

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3181 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3240

3181 agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct cccgaatggt 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3300

3241 agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg tctaaatagt 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3360

3301 cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg aaataggcgg 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... AseI(3374) .......... .......... .......... ..........

3361 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3420

3361 aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg tcaattatca 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 3480

3421 aacgcgttgc aacaacggta acgatgtccg tagcaccaca gtgcgagcag caaaccatac 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3540

3481 cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg gtacaacacg 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3600

3541 ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa ccggcgtcac 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3660

3601 aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg taggcattct 3660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3720

3661 acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac atacgccgct 3720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 3780

3721 ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc gtcttgaaat 3780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3840

3781 tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta gaatggcgac 3840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3900

3841 aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg tagaaaatga 3900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 3960

3901 aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt tttcccttat 3960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 4020

3961 tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat aacttcgtaa 4020

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4080

4021 atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt tttatttgtt 4080

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 4140

4081 tatccccaag gcgcgtgtaa aggggctttt cacggtggac tgcgcgggac atcgccgcgt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 4200

4141 aattcgcgcc gcccacacca ccaatgcgcg tcgcactggc gatgtgaacg gtcgcgggat 4200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 4260

4201 cgcgggcgag gaaagcgaaa gaagggaagg aaagagcggt gcaagcggcc gaaaggggca 4260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 4320

4261 gttcgagatt tagcccccga gggaaatccc aaggctaaat cacgaaatgc cgtggagctg 4320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 4380

4321 gggttttttg aactaatccc actaccaagt gcatcacccg gtagcgggac tatctgccaa 4380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 4440

4381 aaagcgggaa actgcaacct caggtgcaag aaattatcac ctgagaacaa ggtttgacct 4440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 4500

4441 tgttgtgagt tgggatagag ccagataaga aaactaaata ttccctaaaa cggctaaagc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 4560

4501 cggataacca attttttact cgactaaatt gtttttaaat tgcgcttaaa attgttttat 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttaacgttta caatttccca ttcgccattc aggctgcgca actgttggga agggcgatcg 4620

4561 aattgcaaat gttaaagggt aagcggtaag tccgacgcgt tgacaaccct tcccgctagc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag 4680

4621 cacgcccgga gaagcgataa tgcggtcggg ttcgatggta ctattcattc attataattc 4680

.......... .......... .......... .......... .......... ..........

.......... NotI(4700) .......... .......... .......... ..........

4681 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg 4740

4681 catgccctcc atgaacctcg ccggcgttat tttatagaaa taaaagtaat gtagacacac 4740

.......... .......... .......... . synth_PA_term TB up_PA_term .

.......... .......... .......... .......... .......... ..........

4741 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac 4800

4741 aaccaaaaaa cacacttagc tatcatgatt gtatgcgaga ggtagttttg ttttgctttg 4800

synth_PA_term .... TB up_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct 4860

4801 ttttgtttga tcgttttatc cgacaggggt cacgttcacg tccacggtct tgtaaagaga 4860

........ RVpri3_pri TB up_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atcgata... .......... .......... .......... .......... .......... 4867

4861 tagctat... .......... .......... .......... .......... .......... 4867

.......... .......... .......... .......... .......... ..........