A,LOCUS pBigT 6117 bp DNA SYN 29-Aug-2007 DEFINITION pBigT ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6117 /organism="pBigT" /mol_type="other DNA" misc_feature 153..169 /label="pBluescriptSK_primer" misc_feature complement(183..216) /label="loxP" misc_feature complement(281..304) /label="MSCV_rev_primer" gene 756..1556 /label="Neomycin" /gene="Neomycin" CDS 756..1559 /label="Neomycin" /gene="Neomycin" /note="ORF frame 3" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD LDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" terminator 2181..2312 /label="SV40_PA_terminator" misc_feature 2269..2288 /label="EBV_rev_primer" terminator 2435..2566 /label="SV40_PA_terminator" misc_feature 2523..2542 /label="EBV_rev_primer" terminator 2689..2820 /label="SV40_PA_terminator" misc_feature 2777..2796 /label="EBV_rev_primer" misc_feature complement(2849..2882) /label="loxP" misc_feature complement(2918..2934) /label="pBluescriptKS_primer" misc_feature complement(2971..2988) /label="BGH_rev_primer" terminator 2974..3201 /label="bGH_PA_terminator" promoter complement(3273..3292) /label="T3_promoter" misc_feature complement(3309..3327) /label="M13_reverse_primer" misc_feature complement(3326..3348) /label="M13_pUC_rev_primer" promoter complement(3362..3391) /label="lac_promoter" rep_origin complement(3700..4319) /label="pBR322_origin" CDS complement(4474..5334) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4474..5334) /label="Ampicillin" /gene="Ampicillin" promoter complement(5376..5404) /label="AmpR_promoter" rep_origin complement(5597..5903) /label="f1_origin" misc_feature complement(5921..6076) /label="lacZ_a" misc_feature 6047..6069 /label="M13_pUC_fwd_primer" misc_feature 6062..6078 /label="M13_forward20_primer" promoter 6088..6106 /label="T7_promoter" ORIGIN 1 ttaattaagg gatctgtagg gcgcagtagt ccagggtttc cttgatgatg tcatacttat 61 cctgtccctt ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg tctttccagt 121 ggggatcgac ggtatcgtag agtcgaggcc gctctagaac tagtggatcc ggaaccctta 181 atataacttc gtataatgta tgctatacga agttattagg tccctcgacc tgcaggaatt 241 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 301 gctggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 361 agcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag 421 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag 481 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc 541 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa 601 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcgaaggtcc 661 tcccgaggcc cggcattctc gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 721 ttcctcatct ccgggccttt cgacctgcag ccaatatggg atcggccatt gaacaagatg 781 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 841 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 901 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 961 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 1021 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 1081 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 1141 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 1201 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 1261 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg 1321 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 1381 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 1441 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 1501 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 1561 gggatccgct gtaagtctgc agaaattgat gatctattaa acaataaaga tgtccactaa 1621 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt 1681 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc 1741 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt 1801 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct 1861 atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact 1921 gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca 1981 catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa gcttgcagat 2041 ctgcgactct agaggatctg cgactctaga ggatcataat cagccatacc acatttgtag 2101 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 2161 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2221 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2281 aactcatcaa tgtatcttat catgtctgga tctgcgactc tagaggatca taatcagcca 2341 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 2401 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 2461 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 2521 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgcg actctagagg 2581 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 2641 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 2701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 2761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 2821 cccatcaagc tgatccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt 2881 attaggtccc tcgacctgca gcccaagcta gcttatcgat accgtcgacc tcgaggggcc 2941 cccgcgggcg gccgcgagct cgctgatcag cctcgactgt gccttctagt tgccagccat 3001 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 3061 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 3121 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 3181 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgatcctct 3241 agttggcgcg ccaacggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 3301 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 3361 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 3421 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 3481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 3541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4141 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4321 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 4381 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4441 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 4501 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 4561 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 4621 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 4681 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 4741 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 4801 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 4861 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 4921 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 4981 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5041 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 5101 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 5161 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 5221 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 5281 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 5341 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 5401 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 5461 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 5521 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 5581 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 5641 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 5701 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 5761 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 5821 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 5881 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg 5941 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 6001 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 6061 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggcc // pBigT "Times New Roman'CpBluescriptSK_pri"Times New RomanCloxP"Times New RomanCɆ0 MSCV_rev_pri"Times New RomanNeomycin"Times New RomanCORF 3"Times New RomanC  SV40_PA_term"Times New RomanCɆ EBV_rev_pri"Times New Roman   SV40_PA_term"Times New RomanC  EBV_rev_pri"Times New RomanC   SV40_PA_term"Times New RomanCɆ  EBV_rev_pri"Times New Roman! B loxP"Times New RomanCf v pBluescriptKS_pri"Times New RomanC  BGH_rev_pri"Times New RomanCɆ  bGH_PA_term"Times New Roman T3_pro"Times New RomanC M13_reverse_pri"Times New RomanC  M13_pUC_rev_pri"Times New RomanCɆ" ? lac_pro"Times New Romant pBR322_origin"Times New RomanCzORF 1"Times New RomanCz Ampicillin"Times New RomanCɆAmpR_pro"Times New Roman f1_origin"Times New RomanC!lacZ_a"Times New RomanCM13_pUC_fwd_pri"Times New RomanCɆM13_forward20_pri"Times New Roman*T7_pro"Times New Roman EcoRI(236),AgeI(355)"Times New RomanE StuI"Times New Roman3 =GG} NarI(893),MscI(975)"Times New Roman}rC7* * NcoI"Times New Roman- EcoRV"Times New Roman   HindIII"Times New Roman@JSLZ cNheI(2907),ClaI(2916),SalI(2924),XhoI(2930),ApaI(2940),SacII(2945),NotI(2949),SacI(2960),BclI(2964)"Times New Roman[  KpnI"Times New Roman x test"Times New Roman |gg-1 test"Times New Roman8 #%-GGOc test"Times New RomanQ1