Sequence of pBigT

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Sequence of pBigT

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Sequence of pBigT : 6117 BP; 1470 A; 1533 C; 1536 G; 1578 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttaattaagg gatctgtagg gcgcagtagt ccagggtttc cttgatgatg tcatacttat 60

1 aattaattcc ctagacatcc cgcgtcatca ggtcccaaag gaactactac agtatgaata 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 cctgtccctt ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg tctttccagt 120

61 ggacagggaa aaaaaaggtg tcgagcgcca actcctgttt gagaagcgcc agaaaggtca 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggggatcgac ggtatcgtag agtcgaggcc gctctagaac tagtggatcc ggaaccctta 180

121 cccctagctg ccatagcatc tcagctccgg cgagatcttg atcacctagg ccttgggaat 180

.......... .......... .......... ...... pBluescriptSK_pri ......

.......... .......... .......... .......... .......... ..........

181 atataacttc gtataatgta tgctatacga agttattagg tccctcgacc tgcaggaatt 240

181 tatattgaag catattacat acgatatgct tcaataatcc agggagctgg acgtccttaa 240

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 300

241 gatggcccat cccctccgcg aaaagggttc cgtcagacct cgtacgcgaa atcgtcgggg 300

.......... .......... .......... ......... MSCV_rev_pri .........

.......... .......... .......... .......... .......... AgeI(355)

301 gctggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 360

301 cgaccgtgaa ccgcgatgtg ttcaccggag accggagcgt gtgtaaggtg taggtggcca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag 420

361 tcgcggttgg ccgaggcaag aaaccaccgg ggaagcgcgg tggaagatga ggaggggatc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag 480

421 agtccttcaa gggggggcgg ggcgtcgagc gcagcacgtc ctgcactgtt taccttcatc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... StuI(538)

481 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc 540

481 gtgcagagtg atcagagcac gtctacctgt cgtggcgact cgttaccttc gcccatccgg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa 600

541 aaaccccgtc gccggttatc gtcgaaacga ggaagcgaaa gacccgagtc tccgaccctt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcgaaggtcc 660

601 ccccacccag gcccccgccc gagtccccgc ccgagtcccc gccccgcccg cgcttccagg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcccgaggcc cggcattctc gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 720

661 agggctccgg gccgtaagag cgtgcgaagt tttcgcgtgc agacggcgcg acaagaggag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttcctcatct ccgggccttt cgacctgcag ccaatatggg atcggccatt gaacaagatg 780

721 aaggagtaga ggcccggaaa gctggacgtc ggttataccc tagccggtaa cttgttctac 780

.......... .......... .......... ........ Neomycin ORF 3 ........

.......... .......... .......... .......... .......... ..........

781 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 840

781 ctaacgtgcg tccaagaggc cggcgaaccc acctctccga taagccgata ctgacccgtg 840

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 900

841 ttgtctgtta gccgacgaga ctacggcggc acaaggccga cagtcgcgtc cccgcgggcc 900

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 960

901 aagaaaaaca gttctggctg gacaggccac gggacttact tgacgtcctg ctccgtcgcg 960

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... MscI(975) .......... .......... .......... ..........

961 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 1020

961 ccgatagcac cgaccggtgc tgcccgcaag gaacgcgtcg acacgagctg caacagtgac 1020

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 1080

1021 ttcgcccttc cctgaccgac gataacccgc ttcacggccc cgtcctagag gacagtagag 1080

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 1140

1081 tggaacgagg acggctcttt cataggtagt accgactacg ttacgccgcc gacgtatgcg 1140

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 1200

1141 aactaggccg atggacgggt aagctggtgg ttcgctttgt agcgtagctc gctcgtgcat 1200

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 1260

1201 gagcctacct tcggccagaa cagctagtcc tactagacct gcttctcgta gtccccgagc 1260

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg 1320

1261 gcggtcggct tgacaagcgg tccgagttcc gcgcgtacgg gctgccgcta ctagagcagc 1320

.......... ........ Neomycin ORF 3 ........ .......... ..........

NcoI(1325) .......... .......... .......... .......... ..........

1321 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 1380

1321 actgggtacc gctacggacg aacggcttat agtaccacct tttaccggcg aaaagaccta 1380

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 1440

1381 agtagctgac accggccgac ccacaccgcc tggcgatagt cctgtatcgc aaccgatggg 1440

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 1500

1441 cactataacg acttctcgaa ccgccgctta cccgactggc gaaggagcac gaaatgccat 1500

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 1560

1501 agcggcgagg gctaagcgtc gcgtagcgga agatagcgga agaactgctc aagaagactc 1560

.......... ........ Neomycin ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gggatccgct gtaagtctgc agaaattgat gatctattaa acaataaaga tgtccactaa 1620

1561 ccctaggcga cattcagacg tctttaacta ctagataatt tgttatttct acaggtgatt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt 1680

1621 ttaccttcaa aaaggacagt atgaaacaat tcttcccact cttgtctcat ggatgtaaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc 1740

1681 cttaccttcc taacctcgat gcccccaccc ccaccccacc ctaatctatt tacggacgag 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(1791)

1741 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt 1800

1741 aaatgacttc cgagaaatga taacgaaata ctattacaaa gtatcaacct atagtattaa 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct 1860

1801 atttgttcgt tttggtttaa ttcccggtcg agtaaggagg gtgagtacta gatatctaga 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact 1920

1861 tatctagaga gcaccctagt aacaaaaaga gaactaaggg tgaaacacca agattcatga 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca 1980

1921 caccaaaggt ttacacagtc aaagtatcgg acttcttgct ctagtcgtcg gagacaaggt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ........ HindIII(2029) ........

1981 catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa gcttgcagat 2040

1981 gtatgtgaag taagagtcat aacaaaacgg ttcaagatta aggtagtctt cgaacgtcta 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctgcgactct agaggatctg cgactctaga ggatcataat cagccatacc acatttgtag 2100

2041 gacgctgaga tctcctagac gctgagatct cctagtatta gtcggtatgg tgtaaacatc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 2160

2101 tccaaaatga acgaaatttt ttggagggtg tggaggggga cttggacttt gtattttact 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2220

2161 tacgttaaca acaacaattg aacaaataac gtcgaatatt accaatgttt atttcgttat 2220

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2280

2221 cgtagtgttt aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca ccaaacaggt 2280

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

2281 aactcatcaa tgtatcttat catgtctgga tctgcgactc tagaggatca taatcagcca 2340

2281 ttgagtagtt acatagaata gtacagacct agacgctgag atctcctagt attagtcggt 2340

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 2400

2341 atggtgtaaa catctccaaa atgaacgaaa ttttttggag ggtgtggagg gggacttgga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 2460

2401 ctttgtattt tacttacgtt aacaacaaca attgaacaaa taacgtcgaa tattaccaat 2460

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

2461 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 2520

2461 gtttatttcg ttatcgtagt gtttaaagtg tttatttcgt aaaaaaagtg acgtaagatc 2520

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgcg actctagagg 2580

2521 aacaccaaac aggtttgagt agttacatag aatagtacag acctagacgc tgagatctcc 2580

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 2640

2581 tagtattagt cggtatggtg taaacatctc caaaatgaac gaaatttttt ggagggtgtg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 2700

2641 gagggggact tggactttgt attttactta cgttaacaac aacaattgaa caaataacgt 2700

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 2760

2701 cgaatattac caatgtttat ttcgttatcg tagtgtttaa agtgtttatt tcgtaaaaaa 2760

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 2820

2761 agtgacgtaa gatcaacacc aaacaggttt gagtagttac atagaatagt acagacctag 2820

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cccatcaagc tgatccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt 2880

2821 gggtagttcg actaggcctt gggaattata ttgaagcata ttacatacga tatgcttcaa 2880

.......... .......... .......... .......... loxP ..........

.......... .... NheI(2907) ClaI(2916) SalI(2924) XhoI(2930) ....

2881 attaggtccc tcgacctgca gcccaagcta gcttatcgat accgtcgacc tcgaggggcc 2940

2881 taatccaggg agctggacgt cgggttcgat cgaatagcta tggcagctgg agctccccgg 2940

.......... .......... .......... ...... pBluescriptKS_pri ......

SacII(2945),NotI(2949) SacI(2960),BclI(2964) ...... ..........

2941 cccgcgggcg gccgcgagct cgctgatcag cctcgactgt gccttctagt tgccagccat 3000

2941 gggcgcccgc cggcgctcga gcgactagtc ggagctgaca cggaagatca acggtcggta 3000

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

3001 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 3060

3001 gacaacaaac ggggaggggg cacggaagga actgggacct tccacggtga gggtgacagg 3060

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 3120

3061 aaaggattat tttactcctt taacgtagcg taacagactc atccacagta agataagacc 3120

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 3180

3121 ccccacccca ccccgtcctg tcgttccccc tcctaaccct tctgttatcg tccgtacgac 3180

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgatcctct 3240

3181 ccctacgcca cccgagatac cgaagactcc gcctttcttg gtcgaccccg agctaggaga 3240

......... bGH_PA_term ......... .......... .......... ..........

.......... KpnI(3260) .......... .......... .......... ..........

3241 agttggcgcg ccaacggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 3300

3241 tcaaccgcgc ggttgccatg ggtcgaaaac aagggaaatc actcccaatt aacgcgcgaa 3300

.......... .......... .......... .......... T3_pro ..........

.......... .......... .......... .......... .......... ..........

3301 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 3360

3301 ccgcattagt accagtatcg acaaaggaca cactttaaca ataggcgagt gttaaggtgt 3360

....... M13_reverse_pri .... M13_pUC_rev_pri ....... ..........

.......... .......... .......... .......... .......... ..........

3361 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 3420

3361 gttgtatgct cggccttcgt atttcacatt tcggacccca cggattactc actcgattga 3420

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 3480

3421 gtgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca gcacggtcga 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 3540

3481 cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg cgagaaggcg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3600

3541 aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3660

3601 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3720

3661 tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 3720

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3780

3721 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3840

3781 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3900

3841 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3960

3901 cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4020

3961 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4080

4021 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4140

4081 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4200

4141 gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4260

4201 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4320

4261 acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 4380

4321 aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4440

4381 taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa aatttagtta 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 4500

4441 gatttcatat atactcattt gaaccagact gtcaatggtt acgaattagt cactccgtgg 4500

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4501 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 4560

4501 atagagtcgc tagacagata aagcaagtag gtatcaacgg actgaggggc agcacatcta 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 4620

4561 ttgatgctat gccctcccga atggtagacc ggggtcacga cgttactatg gcgctctggg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 4680

4621 tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt cggccttccc ggctcgcgtc 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 4740

4681 ttcaccagga cgttgaaata ggcggaggta ggtcagataa ttaacaacgg cccttcgatc 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 4800

4741 tcattcatca agcggtcaat tatcaaacgc gttgcaacaa cggtaacgat gtccgtagca 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 4860

4801 ccacagtgcg agcagcaaac cataccgaag taagtcgagg ccaagggttg ctagttccgc 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 4920

4861 tcaatgtact agggggtaca acacgttttt tcgccaatcg aggaagccag gaggctagca 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 4980

4921 acagtcttca ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg acgtattaag 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5040

4981 agaatgacag tacggtaggc attctacgaa aagacactga ccactcatga gttggttcag 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 5100

5041 taagactctt atcacatacg ccgctggctc aacgagaacg ggccgcagtt atgccctatt 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 5160

5101 atggcgcggt gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa gaagccccgc 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 5220

5161 ttttgagagt tcctagaatg gcgacaactc taggtcaagc tacattgggt gagcacgtgg 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 5280

5221 gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt tttgtccttc 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 5340

5281 cgttttacgg cgttttttcc cttattcccg ctgtgccttt acaacttatg agtatgagaa 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 5400

5341 ggaaaaagtt ataataactt cgtaaatagt cccaataaca gagtactcgc ctatgtataa 5400

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

5401 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 5460

5401 acttacataa atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 5520

5461 tggatttaac attcgcaatt ataaaacaat tttaagcgca atttaaaaac aatttagtcg 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 5580

5521 agtaaaaaat tggttatccg gctttagccg ttttagggaa tatttagttt tcttatctgg 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 5640

5581 ctctatccca actcacaaca aggtcaaacc ttgttctcag gtgataattt cttgcacctg 5640

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 5700

5641 aggttgcagt ttcccgcttt ttggcagata gtcccgctac cgggtgatgc acttggtagt 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 5760

5701 gggattagtt caaaaaaccc cagctccacg gcatttcgtg atttagcctt gggatttccc 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 5820

5761 tcgggggcta aatctcgaac tgcccctttc ggccgcttgc accgctcttt ccttcccttc 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 5880

5821 tttcgctttc ctcgcccgcg atcccgcgac cgttcacatc gccagtgcga cgcgcattgg 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg 5940

5881 tggtgtgggc ggcgcgaatt acgcggcgat gtcccgcgca gggtaagcgg taagtccgac 5940

.......... f1_origin .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

5941 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 6000

5941 gcgttgacaa cccttcccgc tagccacgcc cggagaagcg ataatgcggt cgaccgcttt 6000

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 6060

6001 ccccctacac gacgttccgc taattcaacc cattgcggtc ccaaaagggt cagtgctgca 6060

.......... .......... lacZ_a .......... ....... M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

6061 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggcc... 6117

6061 acattttgct gccggtcact cgcgcgcatt atgctgagtg atatcccgct taaccgg... 6117

lacZ_a M13_pUC_fwd_pri M13_forward20_pri T7_pro ..........