Sequence of pBlueScript_II

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pBlueScript_II_SK(+)

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pBlueScript II SK(+) : 2961 BP; 746 A; 739 C; 754 G; 722 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60

1 gatttaacat tcgcaattat aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120

61 taaaaaattg gttatccggc tttagccgtt ttagggaata tttagttttc ttatctggct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180

121 ctatcccaac tcacaacaag gtcaaacctt gttctcaggt gataatttct tgcacctgag 180

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240

181 gttgcagttt cccgcttttt ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg 240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300

241 gattagttca aaaaacccca gctccacggc atttcgtgat ttagccttgg gatttccctc 300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360

301 gggggctaaa tctcgaactg cccctttcgg ccgcttgcac cgctctttcc ttcccttctt 360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420

361 tcgctttcct cgcccgcgat cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg 420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480

421 gtgtgggcgg cgcgaattac gcggcgatgt cccgcgcagg gtaagcggta agtccgacgc 480

.......... f1_origin .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540

481 gttgacaacc cttcccgcta gccacgcccg gagaagcgat aatgcggtcg accgctttcc 540

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600

541 ccctacacga cgttccgcta attcaaccca ttgcggtccc aaaagggtca gtgctgcaac 600

.......... .......... lacZ_a .......... ....... M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 660

601 attttgctgc cggtcactcg cgcgcattat gctgagtgat atcccgctta acccatggcc 660

lacZ_a M13_forward20_pri ...... T7_pro .......... ..........

ApaI,XhoI SalI ClaI,HindIII EcoRV(697),EcoRI(701)

661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg 720

661 cgggggggag ctccagctgc catagctatt cgaactatag cttaaggacg tcgggccccc 720

...... pBluescriptKS_pri ...... .......... .......... ..........

SpeI XbaI NotI738),EagI(738) SacII(750) SacI(759)

721 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt 780

721 taggtgatca agatctcgcc ggcggtggcg ccacctcgag gtcgaaaaca agggaaatca 780

pBluescriptSK_pri .. .......... .......... .......... T3_pro

.......... .......... .......... .......... .......... ..........

781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 840

781 ctcccaatta acgcgcgaac cgcattagta ccagtatcga caaaggacac actttaacaa 840

T3_pro .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 900

841 taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac 900

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 960

901 ggattactca ctcgattgag tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 1020

961 ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 1080

1021 cataacccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 1140

1081 ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1200

1141 tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1260

1201 gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1320

1261 gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1380

1321 cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1440

1381 agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1500

1441 tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1560

1501 ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1620

1561 gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 1680

1621 acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag acgcgagacg 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 1740

1681 acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 1800

1741 gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 1860

1801 ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa 1860

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 1920

1861 ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa aatttaattt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 1980

1921 ttacttcaaa atttagttag atttcatata tactcatttg aaccagactg tcaatggtta 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 2040

1981 cgaattagtc actccgtgga tagagtcgct agacagataa agcaagtagg tatcaacgga 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2100

2041 ctgaggggca gcacatctat tgatgctatg ccctcccgaa tggtagaccg gggtcacgac 2100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 2160

2101 gttactatgg cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat ttggtcggtc 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 2220

2161 ggccttcccg gctcgcgtct tcaccaggac gttgaaatag gcggaggtag gtcagataat 2220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 2280

2221 taacaacggc ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg ttgcaacaac 2280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 2340

2281 ggtaacgatg tccgtagcac cacagtgcga gcagcaaacc ataccgaagt aagtcgaggc 2340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 2400

2341 caagggttgc tagttccgct caatgtacta gggggtacaa cacgtttttt cgccaatcga 2400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 2460

2401 ggaagccagg aggctagcaa cagtcttcat tcaaccggcg tcacaatagt gagtaccaat 2460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 2520

2461 accgtcgtga cgtattaaga gaatgacagt acggtaggca ttctacgaaa agacactgac 2520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 2580

2521 cactcatgag ttggttcagt aagactctta tcacatacgc cgctggctca acgagaacgg 2580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 2640

2581 gccgcagtta tgccctatta tggcgcggtg tatcgtcttg aaattttcac gagtagtaac 2640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 2700

2641 cttttgcaag aagccccgct tttgagagtt cctagaatgg cgacaactct aggtcaagct 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 2760

2701 acattgggtg agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg tcgcaaagac 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 2820

2761 ccactcgttt ttgtccttcc gttttacggc gttttttccc ttattcccgc tgtgccttta 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 2880

2821 caacttatga gtatgagaag gaaaaagtta taataacttc gtaaatagtc ccaataacag 2880

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 2940

2881 agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc caaggcgcgt 2940

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 catttccccg aaaagtgcca c......... .......... .......... .......... 2961

2941 gtaaaggggc ttttcacggt g......... .......... .......... .......... 2961

.......... .......... .......... .......... .......... ..........