Sequence of pBlue

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Sequence of pBlue_TOPO

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Sequence of pBlue TOPO : 7793 BP; 1758 A; 2070 C; 2112 G; 1853 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctaat acgactcact atagggagac ccaagctggc tagcgtttaa 60

1 ctgcctagcc ctctagatta tgctgagtga tatccctctg ggttcgaccg atcgcaaatt 60

.......... .......... T7_pro .......... .......... ..........

.......... ...... BamHI(83) SpeI(89) ...... .......... ..........

61 acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt gcccttaagg 120

61 tgaattcgaa ccatggctcg agcctaggtg atcaggtcac accaccttaa cgggaattcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcaattctgc agaaagctta ccatgggggg ttctcatcat catcatcatc atggtatggc 180

121 cgttaagacg tctttcgaat ggtacccccc aagagtagta gtagtagtag taccataccg 180

.......... .......... .......... ......... ORF 2 6xHis .........

.......... .......... .......... .......... .......... ..........

181 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggtacc 240

181 atcgtactga ccacctgtcg tttacccagc cctagacatg ctgctactgc tattccatgg 240

Xpress_fwd_pri T7_gene10_leader T7_leader ORF 2 Xpress_EK

.......... .......... .......... .......... .......... ..........

241 taaggatcag cttggagttg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg 300

241 attcctagtc gaacctcaac tagggcagca aaatgttgca gcactgaccc ttttgggacc 300

.......... ....... ORF 2 M13_pUC_fwd_pri lacZ .......

.......... .......... .......... .......... .......... ..........

301 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 360

301 gcaatgggtt gaattagcgg aacgtcgtgt agggggaaag cggtcgaccg cattatcgct 360

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt 420

361 tctccgggcg tggctagcgg gaagggttgt caacgcgtcg gacttaccgc ttaccgcgaa 420

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga 480

421 acggaccaaa ggccgtggtc ttcgccacgg cctttcgacc gacctcacgc tagaaggact 480

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta 540

481 ccggctatga cagcagcagg ggagtttgac cgtctacgtg ccaatgctac gcgggtagat 540

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac 600

541 gtggttgcat tggatagggt aatgccagtt aggcggcaaa caagggtgcc tcttaggctg 600

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg 660

601 cccaacaatg agcgagtgta aattacaact actttcgacc gatgtccttc cggtctgcgc 660

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg 720

661 ttaataaaaa ctaccgcaat tgagccgcaa agtagacacc acgttgcccg cgacccagcc 720

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg 780

721 aatgccggtc ctgtcagcaa acggcagact taaactggac tcgcgtaaaa atgcgcggcc 780

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc tggaagatca 840

781 tcttttggcg gagcgccact accacgacgc aacctcactg ccgtcaatag accttctagt 840

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac 900

841 cctatacacc gcctactcgc cgtaaaaggc actgcagagc aacgacgtat ttggctgatg 900

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact 960

901 tgtttagtcg ctaaaggtac aacggtgagc gaaattacta ctaaagtcgg cgcgacatga 960

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt 1020

961 cctccgactt caagtctaca cgccgctcaa cgcactgatg gatgcccatt gtcaaagaaa 1020

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ClaI(1077)

1021 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga 1080

1021 taccgtccca ctttgcgtcc agcggtcgcc gtggcgcgga aagccgccac tttaatagct 1080

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact 1140

1081 actcgcacca ccaatacggc tagcgcagtg tgatgcagac ttgcagcttt tgggctttga 1140

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg 1200

1141 cacctcgcgg ctttagggct tagagatagc acgccaccaa cttgacgtgt ggcggctgcc 1200

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg 1260

1201 gtgcgactaa cttcgtcttc ggacgctaca gccaaaggcg ctccacgcct aacttttacc 1260

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca 1320

1261 agacgacgac gacttgccgt tcggcaacga ctaagctccg caattggcag tgctcgtagt 1320

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... ......... EcoRV(1366) .........

1321 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa 1380

1321 aggagacgta ccagtccagt acctactcgt ctgctaccac gtcctatagg acgactactt 1380

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac 1440

1381 cgtcttgttg aaattgcggc acgcgacaag cgtaataggc ttggtaggcg acaccatgtg 1440

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat 1500

1441 cgacacgctg gcgatgccgg acatacacca cctacttcgg ttataacttt gggtgccgta 1500

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt 1560

1501 ccacggttac ttagcagact ggctactagg cgcgaccgat ggccgctact cgcttgcgca 1560

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa 1620

1561 ttgcgcttac cacgtcgcgc tagcattagt gggctcacac tagtagacca gcgacccctt 1620

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc 1680

1621 acttagtccg gtgccgcgat tagtgctgcg cgacatagcg acctagttta gacagctagg 1680

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg 1740

1681 aagggcgggc cacgtcatac ttccgccgcc tcggctgtgg tgccggtggc tataataaac 1740

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat 1800

1741 gggctacatg cgcgcgcacc tacttctggt cgggaagggc cgacacggct ttaccaggta 1800

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca 1860

1801 gttttttacc gaaagcgatg gacctctctg cgcgggcgac taggaaacgc ttatgcgggt 1860

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc 1920

1861 gcgctaccca ttgtcagaac cgccaaagcg atttatgacc gtccgcaaag cagtcatagg 1920

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga 1980

1921 ggcaaatgtc ccgccgaagc agaccctgac ccacctagtc agcgactaat ttatactact 1980

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca 2040

1981 tttgccgttg ggcaccagcc gaatgccgcc actaaaaccg ctatgcggct tgctagcggt 2040

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc 2100

2041 caagacatac ttgccagacc agaaacggct ggcgtgcggc gtaggtcgcg actgccttcg 2100

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag 2160

2101 ttttgtggtc gtcgtcaaaa aggtcaaggc aaataggccc gtttggtagc ttcactggtc 2160

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg 2220

2161 gcttatggac aaggcagtat cgctattgct cgaggacgtg acctaccacc gcgacctacc 2220

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat 2280

2221 attcggcgac cgttcgccac ttcacggaga cctacagcga ggtgttccat ttgtcaacta 2280

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt 2340

2281 acttgacgga cttgatggcg tcggcctctc gcggcccgtt gagaccgagt gtcatgcgca 2340

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg 2400

2341 tcacgttggc ttgcgctggc gtaccagtct tcggcccgtg tagtcgcgga ccgtcgtcac 2400

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct 2460

2401 cgcagaccgc cttttggagt cacactgcga ggggcggcgc agggtgcggt agggcgtaga 2460

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg 2520

2461 ctggtggtcg ctttacctaa aaacgtagct cgacccatta ttcgcaaccg ttaaattggc 2520

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct 2580

2521 ggtcagtccg aaagaaagtg tctacaccta accgctattt tttgttgacg actgcggcga 2580

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg 2640

2581 cgcgctagtc aagtgggcac gtggcgacct attgctgtaa ccgcattcac ttcgctgggc 2640

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc 2700

2641 gtaactggga ttgcggaccc agcttgcgac cttccgccgc ccggtaatgg tccggcttcg 2700

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca 2760

2701 tcgcaacaac gtcacgtgcc gtctatgtga acgactacgc cacgactaat gctggcgagt 2760

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg 2820

2761 gcgcaccgtc gtagtcccct tttggaataa atagtcggcc ttttggatgg cctaactacc 2820

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc 2880

2821 atcaccagtt taccgctaat ggcaactaca acttcaccgc tcgctatgtg gcgtaggccg 2880

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt 2940

2881 cgcctaaccg gacttgacgg tcgaccgcgt ccatcgtctc gcccatttga ccgagcctaa 2940

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct 3000

2941 tcccggcgtt cttttgatag ggctggcgga atgacggcgg acaaaactgg cgaccctaga 3000

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg 3060

3001 cggtaacagt ctgtacatat ggggcatgca gaagggctcg cttttgccag acgcgacgcc 3060

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag 3120

3061 ctgcgcgctt aacttaatac cgggtgtggt caccgcgccg ctgaaggtca agttgtagtc 3120

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga 3180

3121 ggcgatgtca gttgtcgttg actacctttg gtcggtagcg gtagacgacg tgcgccttct 3180

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag 3240

3181 tccgtgtacc gacttatagc tgccaaaggt atacccctaa ccaccgctgc tgaggacctc 3240

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cccgtcagta tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg 3300

3241 gggcagtcat agccgcctta atgtcgactc gcggccagcg atggtaatgg tcaaccagac 3300

.......... .......... ORF 2 lacZ .......... .......... ..........

.......... .......... .......... NotI(3340) XhoI(3346) XbaI(3352)

3301 gtgtcaaaaa taataaagcc gaattatcca gcacagtggc ggccgctcga gtctagaggg 3360

3301 cacagttttt attatttcgg cttaataggt cgtgtcaccg ccggcgagct cagatctccc 3360

ORF 2 lacZ .......... .......... .......... .......... ..........

ApaI(3362) .......... .......... .......... .......... ..........

3361 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 3420

3361 gggcaaattt gggcgactag tcggagctga cacggaagat caacggtcgg tagacaacaa 3420

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

3421 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 3480

3421 acggggaggg ggcacggaag gaactgggac cttccacggt gagggtgaca ggaaaggatt 3480

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 3540

3481 attttactcc tttaacgtag cgtaacagac tcatccacag taagataaga ccccccaccc 3540

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 3600

3541 caccccgtcc tgtcgttccc cctcctaacc cttctgttat cgtccgtacg acccctacgc 3600

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 3660

3601 cacccgagat accgaagact ccgcctttct tggtcgaccc cgagatcccc cataggggtg 3660

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 3720

3661 cgcgggacat cgccgcgtaa ttcgcgccgc ccacaccacc aatgcgcgtc gcactggcga 3720

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 3780

3721 tgtgaacggt cgcgggatcg cgggcgagga aagcgaaaga agggaaggaa agagcggtgc 3780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt 3840

3781 aagcggccga aaggggcagt tcgagattta gccccgtagg gaaatcccaa ggctaaatca 3840

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 3900

3841 cgaaatgccg tggagctggg gttttttgaa ctaatcccac taccaagtgc atcacccggt 3900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 3960

3901 agcgggacta tctgccaaaa agcgggaaac tgcaacctca ggtgcaagaa attatcacct 3960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 4020

3961 gagaacaagg tttgaccttg ttgtgagttg ggatagagcc agataagaaa actaaatatt 4020

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4080

4021 ccctaaaacc cctaaagccg gataaccaat tttttactcg actaaattgt ttttaaattg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 4140

4081 cgcttaatta agacacctta cacacagtca atcccacacc tttcaggggt ccgaggggtc 4140

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

4141 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4200

4141 cgtccgtctt catacgtttc gtacgtagag ttaatcagtc gttggtccac acctttcagg 4200

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4260

4201 ggtccgaggg gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtat 4260

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4320

4261 cagggcgggg attgaggcgg gtagggcggg gattgaggcg ggtcaaggcg ggtaagaggc 4320

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

4321 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 4380

4321 ggggtaccga ctgattaaaa aaaataaata cgtctccggc tccggcggag acggagactc 4380

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... StuI(4415) .......... SmaI(4439)

4381 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 4440

4381 gataaggtct tcatcactcc tccgaaaaaa cctccggatc cgaaaacgtt tttcgagggc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 4500

4441 cctcgaacat ataggtaaaa gcctagacta gttctctgtc ctactcctag caaagcgtac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 4560

4501 taacttgttc tacctaacgt gcgtccaaga ggccggcgaa cccacctctc cgataagccg 4560

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 4620

4561 atactgaccc gtgttgtctg ttagccgacg agactacggc ggcacaaggc cgacagtcgc 4620

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 4680

4621 gtccccgcgg gccaagaaaa acagttctgg ctggacaggc cacgggactt acttgacgtc 4680

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... MscI(4708) .......... .......... ..........

4681 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 4740

4681 ctgctccgtc gcgccgatag caccgaccgg tgctgcccgc aaggaacgcg tcgacacgag 4740

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 4800

4741 ctgcaacagt gacttcgccc ttccctgacc gacgataacc cgcttcacgg ccccgtccta 4800

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 4860

4801 gaggacagta gagtggaacg aggacggctc tttcataggt agtaccgact acgttacgcc 4860

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 4920

4861 gccgacgtat gcgaactagg ccgatggacg ggtaagctgg tggttcgctt tgtagcgtag 4920

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 4980

4921 ctcgctcgtg catgagccta ccttcggcca gaacagctag tcctactaga cctgcttctc 4980

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 5040

4981 gtagtccccg agcgcggtcg gcttgacaag cggtccgagt tccgcgcgta cgggctgccg 5040

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 5100

5041 ctcctagagc agcactgggt accgctacgg acgaacggct tatagtacca ccttttaccg 5100

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 5160

5101 gcgaaaagac ctaagtagct gacaccggcc gacccacacc gcctggcgat agtcctgtat 5160

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 5220

5161 cgcaaccgat gggcactata acgacttctc gaaccgccgc ttacccgact ggcgaaggag 5220

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 5280

5221 cacgaaatgc catagcggcg agggctaagc gtcgcgtagc ggaagatagc ggaagaactg 5280

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... ......... BstBI(5308) ......... .......... ..........

5281 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 5340

5281 ctcaagaaga ctcgccctga gaccccaagc tttactggct ggttcgctgc gggttggacg 5340

ORF 1 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 5400

5341 gtagtgctct aaagctaagg tggcggcgga agatactttc caacccgaag ccttagcaaa 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 5460

5401 aggccctgcg gccgacctac taggaggtcg cgcccctaga gtacgacctc aagaagcggg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5520

5461 tggggttgaa caaataacgt cgaatattac caatgtttat ttcgttatcg tagtgtttaa 5520

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

5521 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa c tcatcaatg 5580

5521 agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt gagtagttac 5580

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

5581 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 5640

5581 atagaatagt acagacatat ggcagctgga gatcgatctc gaaccgcatt agtaccagta 5640

.......... .......... .......... .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

5641 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 5700

5641 tcgacaaagg acacacttta acaataggcg agtgttaagg tgtgttgtat gctcggcctt 5700

M13_reverse_pri M13_pUC_rev_pri ........ .......... lac_pro

.......... .......... .......... .......... .......... ..........

5701 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 5760

5701 cgtatttcac atttcggacc ccacggatta ctcactcgat tgagtgtaat taacgcaacg 5760

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 5820

5761 cgagtgacgg gcgaaaggtc agccctttgg acagcacggt cgacgtaatt acttagccgg 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 5880

5821 ttgcgcgccc ctctccgcca aacgcataac ccgcgagaag gcgaaggagc gagtgactga 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 5940

5881 gcgacgcgag ccagcaagcc gacgccgctc gccatagtcg agtgagtttc cgccattatg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6000

5941 ccaataggtg tcttagtccc ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 6060

6001 tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag gcggggggac 6060

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

6061 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 6120

6061 tgctcgtagt gtttttagct gcgagttcag tctccaccgc tttgggctgt cctgatattt 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6180

6121 ctatggtccg caaaggggga ccttcgaggg agcacgcgag aggacaaggc tgggacggcg 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 6240

6181 aatggcctat ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga gttacgagtg 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 6300

6241 cgacatccat agagtcaagc cacatccagc aagcgaggtt cgacccgaca cacgtgcttg 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6360

6301 gggggcaagt cgggctggcg acgcggaata ggccattgat agcagaactc aggttgggcc 6360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6361 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 6420

6361 attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca 6420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 6480

6421 tacatccgcc acgatgtctc aagaacttca ccaccggatt gatgccgatg tgatcttcct 6480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 6540

6481 gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct caaccatcga 6540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6600

6541 gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct 6600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6660

6601 aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc cccagactgc 6660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 6720

6661 gagtcacctt gcttttgagt gcaattccct aaaaccagta ctctaatagt ttttcctaga 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 6780

6721 agtggatcta ggaaaattta atttttactt caaaatttag ttagatttca tatatactca 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 6840

6781 tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt cgctagacag 6840

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

6841 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 6900

6841 ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc tatgccctcc 6900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 6960

6901 cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt ggccgaggtc 6960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 7020

6961 taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa 7020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 7080

7021 ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca tcaagcggtc 7080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 7140

7081 aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt gcgagcagca 7140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 7200

7141 aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt actagggggt 7200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 7260

7201 acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct tcattcaacc 7260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 7320

7261 ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga cagtacggta 7320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 7380

7321 ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact cttatcacat 7380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 7440

7381 acgccgctgg ctcaacgaga acgggccgca gttatgccct attatggcgc ggtgtatcgt 7440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 7500

7441 cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag agttcctaga 7500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 7560

7501 atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact agaagtcgta 7560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 7620

7561 gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta cggcgttttt 7620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 7680

7621 tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa gttataataa 7680

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 7740

7681 cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca taaatctttt 7740

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

7741 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc....... 7793

7741 tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg cag....... 7793

.......... .......... .......... .......... .......... ..........