Sequence of pBridge

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Sequence of pBridge

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Sequence of pBridge : 6526 BP; 1828 A; 1473 C; 1341 G; 1884 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 60

1 cgaacgtacg ttgaagaaaa gaaaaaaaaa gaaaagagag agggggcaac aacagagtgg 60

.......... .......... .......... yADH1_pro .......... ..........

.......... .......... .......... .......... .......... ..........

61 atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac 120

61 tataggcgtt actgtttttt tactaccttc tgtgatttcc ttttttaatt gctgtttctg 120

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact 180

121 tcgtggttgt ctacagcaac aaggtctcga ctactcccca tagagcttcg tgtgctttga 180

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt 240

181 aaaaggaagg aagtaagtgc gtgtgatgag agattactcg ttgccatatg ccggaaggaa 240

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga 300

241 ggtcaatgaa cttaaacttt attttttttc aaacgacaga acgatagttc atatttatct 300

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 360

301 ggacgttaat aattagaaaa caaaggagca gtaacaagag caagggaaag aaggaacaaa 360

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca 420

361 gaaaaagacg tgttataaag ttcgatatgg ttcgtatgtt agttgaggtt cgaacttcgt 420

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact 480

421 tcggaggact ttctacttcg atgacagaag atagcttgtt cgtacgctat aaacggctga 480

.......... .......... .......... Gal4_DBD .......... ..........

.......... .......... .......... .......... .......... ..........

481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg 540

481 atttttcgag ttcacgaggt ttctttttgg cttcacgcgg ttcacagact tcttgttgac 540

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga 600

541 cctcacagcg atgagagggt tttggttttc cagaggcgac tgatcccgtg tagactgtct 600

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga 660

601 tcaccttagt tccgatcttt ctgaccttgt cgataaagat gactaaaaag gagctcttct 660

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(711)

661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt 720

661 ggaactgtac taaaactttt acctaagaaa tgtcctatat tttcgtaaca attgtcctaa 720

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac 780

721 taaacatgtt ctattacact tatttctacg gcagtgtcta tctaaccgaa gtcacctctg 780

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag 840

781 actatacgga gattgtaact ctgtcgtatc ttattcacgc tgtagtagta gccttctctc 840

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... ..... EcoRI(878) SmaI(885),BamHI(888) .....

841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccgtcgacc 900

841 atcattgttt ccagtttctg tcaactgaca tagcggcctt aagggcccct aggcagctgg 900

Gal4_N_term_pri Gal4_DBD .... .......... .......... ..........

PstI(904) .......... .......... .......... .......... ..........

901 tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag 960

901 acgtcggttc gattaaggcc cgcttaaaga atactaaata ctaaaaataa taatttattc 960

.......... .......... .......... yADH1_term .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat 1020

961 aatatttttt ttattcacat atgtttaaaa tttcactgag aatccaaaat tttgctttta 1020

.......... .. yADH1_term pHybLex_rev_pri .. .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg 1080

1021 agaacaagaa ctcattgaga aaggacatcc agtccaacga aagagtccat atcgtactcc 1080

.......... .......... yADH1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcgctcttat tgaccacacc tctaccggca tgccggcaag tgcacaaaca atacttaaat 1140

1081 agcgagaata actggtgtgg agatggccgt acggccgttc acgtgtttgt tatgaattta 1140

.......... yADH1_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta 1200

1141 tttatgatga gtcattattg gataaagaat cgtaaaaact gctttaaacg ataaaacaat 1200

.......... .......... .......... ORF 3 TRP1 .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt 1260

1201 ctcagaaaat gtggtaaaca gaggtgtgga ggcgaatgta gttgtggtta ttgcggtaaa 1260

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag 1320

1261 ttagattcgc gtagtggttg taaaagaccg cagtcaggtg gtcgattgta ttttacattc 1320

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca 1380

1321 gaaagccccg agagaacgga aggttgggtc agtctttagc tcaaggttag gttttcaagt 1380

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct 1440

1381 ggacagggtg gacgaagact tagtttgttc ccttatttgc ttactccaaa gacacttcga 1440

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg 1500

1441 cgtgactcat catacaacgt cagaaaacct ttatgctcag aaaattattg accgtttggc 1500

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg 1560

1501 tccttgagaa ccataagaac ggtgctgagt agaggtacgt caacctgcta tagttacggc 1560

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag 1620

1561 attagtaact ggtctcggtt ttgtaggagg aatccaacta atgctttgtg cggttggttc 1620

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca 1680

1621 ataaagcctc acggacttga taaaaatata cgaaaatgtt ctgaacttta aaaggaacgt 1680

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca 1740

1681 tattggccca gttaacaaga gaaagataac ccgtgtgtat attatgggtc gttcagtcgt 1740

.......... .......... ORF 3 TRP1 .......... .......... ..........

XbaI(1747) .......... .......... .......... .......... ..........

1741 tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat 1800

1741 agccttagat ctcgtgtaag acgccggaga cacgagacgt tcggcgtttg aaagtggtta 1800

.......... .......... ORF 3 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta 1860

1801 cctggtcttg atggacactt taattattgt ctgtatgagg ttcgacggaa acacacgaat 1860

.......... ORF 3 TRP1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt 1920

1861 tagtgcatat gagtgcacga gttatcagtg gttacgggag ggagaaccgg gagaggaaaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cttttttcga ccgaattaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 1980

1921 gaaaaaagct ggcttaatta agcattagta cagtatcgac aaaggacaca ctttaacaat 1980

.......... .......... .......... .......... ....... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

1981 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 2040

1981 aggcgagtgt taaggtgtgt tgtatgctcg gccttcgtat ttcacatttc ggaccccacg 2040

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 2100

2041 gattactcac tccattgagt gtaattaacg caacgcgagt gacgggcgaa aggtcagccc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaacctgtcg tgccaggaag atccgaggcc tagcttctaa ttcttccaac atacaatggg 2160

2101 tttggacagc acggtccttc taggctccgg atcgaagatt aagaaggttg tatgttaccc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agtttggccg agtggtttaa ggcgtcagat ttaggtggat ttaacctcta aaatctctga 2220

2161 tcaaaccggc tcaccaaatt ccgcagtcta aatccaccta aattggagat tttagagact 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tatcttcgga tgcaagggtt cgaatccctt agctctcatt attttttgct ttttctcttg 2280

2221 atagaagcct acgttcccaa gcttagggaa tcgagagtaa taaaaaacga aaaagagaac 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aggtcacatg atcgcaaaat ggcaaatggc acgtgaagct gtcgatattg gggaactgtg 2340

2281 tccagtgtac tagcgtttta ccgtttaccg tgcacttcga cagctataac cccttgacac 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NarI(2374) .......... ..........

2341 gtggttggca aatgactaat taagttagtc aaggcgccat cctcatgaaa actgtgtaac 2400

2341 caccaaccgt ttactgatta attcaatcag ttccgcggta ggagtacttt tgacacattg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ataataaccg aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa 2460

2401 tattattggc ttcacagctt ttccaccgtg gaacaggtta acttgtgcga gctacttttt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aataagatat atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct 2520

2461 ttattctata tatattccaa ttcatttcgc agacaatctt tccttcaaaa aggaaaaaga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgctctcttg tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata 2580

2521 acgagagaac agaaaagtag atgataaagg aagcacatta tgtcccagca gtctatgtat 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gatacaattc tattaccccc atccatacaa tgggccatat ggcttctagc tatccttatg 2640

2581 ctatgttaag ataatggggg taggtatgtt acccggtata ccgaagatcg ataggaatac 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acgtgcctga ctatgccagc ctgggaggac cttctagtcc taagaagaag agaaaggtgg 2700

2641 tgcacggact gatacggtcg gaccctcctg gaagatcagg attcttcttc tctttccacc 2700

.......... .......... .......... .......... .......... ..........

NotI(2701),EagI(2701) BglII(2718) ...... .......... ..........

2701 cggccgcatt agcccgaaga tcttcgggct gatctcccat gtctctactg gtggtggtgc 2760

2701 gccggcgtaa tcgggcttct agaagcccga ctagagggta cagagatgac caccaccacg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttctttggaa ttattggaag gtaaggaatt gccaggtgtt gctttcttat ccgaaaagaa 2820

2761 aagaaacctt aataaccttc cattccttaa cggtccacaa cgaaagaata ggcttttctt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... ClaI(2842) .......... .......... ..........

2821 ataaattgaa ttgaattgaa atcgatagat caattttttt cttttctctt tccccatcct 2880

2821 tatttaactt aacttaactt tagctatcta gttaaaaaaa gaaaagagaa aggggtagga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttacgctaaa ataatagttt attttatttt ttgaatattt tttatttata tacgtatata 2940

2881 aatgcgattt tattatcaaa taaaataaaa aacttataaa aaataaatat atgcatatat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tagactatta tttatctttt aatgattatt aagattttta ttaaaaaaaa attcgctcct 3000

2941 atctgataat aaatagaaaa ttactaataa ttctaaaaat aatttttttt taagcgagga 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttttaatgc ctttatgcag tttttttttc ccattcgata tttctatgtt cgggttcagc 3060

3001 gaaaattacg gaaatacgtc aaaaaaaaag ggtaagctat aaagatacaa gcccaagtcg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtattttaag tttaataact cgaaaattct gcgttcgtta aagctaggcc tcggatcttc 3120

3061 cataaaattc aaattattga gcttttaaga cgcaagcaat ttcgatccgg agcctagaag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctgcattaat gaatcggcca acgcgcgggg agaggcgttt gcgtattggg cgctcttccg 3180

3121 gacgtaatta cttagccggt tgcgcgcccc tctccgcaaa cgcataaccc gcgagaaggc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 3240

3181 gaaggagcga gtgactgagc gacgcgagcc agcaagccga cgccgctcgc catagtcgag 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 3300

3241 tgagtttccg ccattatgcc aataggtgtc ttagtcccct attgcgtcct ttcttgtaca 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3360

3301 ctcgttttcc ggtcgttttc cggtccttgg catttttccg gcgcaacgac cgcaaaaagg 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3420

3361 tatccgaggc ggggggactg ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3480

3421 tgggctgtcc tgatatttct atggtccgca aagggggacc ttcgagggag cacgcgagag 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3540

3481 gacaaggctg ggacggcgaa tggcctatgg acaggcggaa agagggaagc ccttcgcacc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3600

3541 gcgaaagagt atcgagtgcg acatccatag agtcaagcca catccagcaa gcgaggttcg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3660

3601 acccgacaca cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg ccattgatag 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3720

3661 cagaactcag gttgggccat tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3780

3721 cctaatcgtc tcgctccata catccgccac gatgtctcaa gaacttcacc accggattga 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3840

3781 tgccgatgtg atcttcttgt cataaaccat agacgcgaga cgacttcggt caatggaagc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3900

3841 ctttttctca accatcgaga actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3960

3901 aacaaacgtt cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta ggaaactaga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 4020

3961 aaagatgccc cagactgcga gtcaccttgc ttttgagtgc aattccctaa aaccagtact 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 4080

4021 ctaatagttt ttcctagaag tggatctagg aaaatttaat ttttacttca aaatttagtt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 4140

4081 agatttcata tatactcatt tgaaccagac tgtcaatggt tacgaattag tcactccgtg 4140

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4141 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4200

4141 gatagagtcg ctagacagat aaagcaagta ggtatcaacg gactgagggg cagcacatct 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4260

4201 attgatgcta tgccctcccg aatggtagac cggggtcacg acgttactat ggcgctctgg 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4320

4261 gtgcgagtgg ccgaggtcta aatagtcgtt atttggtcgg tcggccttcc cggctcgcgt 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4380

4321 cttcaccagg acgttgaaat aggcggaggt aggtcagata attaacaacg gcccttcgat 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4440

4381 ctcattcatc aagcggtcaa ttatcaaacg cgttgcaaca acggtaacga tgtccgtagc 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4500

4441 accacagtgc gagcagcaaa ccataccgaa gtaagtcgag gccaagggtt gctagttccg 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4560

4501 ctcaatgtac tagggggtac aacacgtttt ttcgccaatc gaggaagcca ggaggctagc 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4620

4561 aacagtcttc attcaaccgg cgtcacaata gtgagtacca ataccgtcgt gacgtattaa 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4680

4621 gagaatgaca gtacggtagg cattctacga aaagacactg accactcatg agttggttca 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4740

4681 gtaagactct tatcacatac gccgctggct caacgagaac gggccgcagt tatgccctat 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4800

4741 tatggcgcgg tgtatcgtct tgaaattttc acgagtagta accttttgca agaagccccg 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4860

4801 cttttgagag ttcctagaat ggcgacaact ctaggtcaag ctacattggg tgagcacgtg 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4920

4861 ggttgactag aagtcgtaga aaatgaaagt ggtcgcaaag acccactcgt ttttgtcctt 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4980

4921 ccgttttacg gcgttttttc ccttattccc gctgtgcctt tacaacttat gagtatgaga 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 5040

4981 aggaaaaagt tataataact tcgtaaatag tcccaataac agagtactcg cctatgtata 5040

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

5041 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 5100

5041 aacttacata aatcttttta tttgtttatc cccaaggcgc gtgtaaaggg gcttttcacg 5100

.......... .......... .......... .......... .......... ..........

AatII(5110) ........ .......... .......... .......... ..........

5101 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 5160

5101 gtggactgca gattctttgg taataatagt actgtaattg gatattttta tccgcatagt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 5220

5161 gctccgggaa agcagagcgc gcaaagccac tactgccact tttggagact gtgtacgtcg 5220

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

5221 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 5280

5221 agggcctctg ccagtgtcga acagacattc gcctacggcc ctcgtctgtt cgggcagtcc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 5340

5281 cgcgcagtcg cccacaaccg cccacagccc cgaccgaatt gatacgccgt agtctcgtct 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttgtactgag agtgcaccat aacgcattta agcataaaca cgcactatgc cgttcttctc 5400

5341 aacatgactc tcacgtggta ttgcgtaaat tcgtatttgt gcgtgatacg gcaagaagag 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat 5460

5401 tacatatata tatatgtccg ttgtgcgtct atatccacgc tgcacttgtc actcgacata 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct 5520

5461 cacgcgtcga gcgcaacgta aaagccttcg cgagcaaaag cctttgcgaa acttcaagga 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 attccgaagt tcctattctc tagctagaaa gtataggaac ttcagagcgc ttttgaaaac 5580

5521 taaggcttca aggataagag atcgatcttt catatccttg aagtctcgcg aaaacttttg 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct 5640

5581 gttttcgcga gacttctgcg tgaaagtttt ttggtttttg cgtggcctga cattgctcga 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat 5700

5641 tgattttata acgcttatgg cgaaggtgtt tgtaacgagt tttcatagag aaacgatata 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat 5760

5701 tagagacacg atatagggat atattggatg ggtaggtgga aagcgaggaa cttgaacgta 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa 5820

5761 gatttgagct ggagatgtaa aaaatacaaa tagagatcat aatgagaaat ctgttttttt 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct 5880

5821 aacatcattc ttgataagta tctcacttag cttttgttat gcttttacat ttgtaaagga 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga 5940

5881 tatgcatcat atatctctgt tttatcttct ttggcaagta ttaaaagact ggttacttct 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata 6000

5941 tagtagttgc gatagtgaaa gacaagtgtt tcatacgcgt taggtgtagc catatcttat 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa 6060

6001 attagcccct acggaaatag aactttttta cgtgggcgtc gaagcgatca ttagtcattt 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc 6120

6061 gcgcccttca cctcagtccg aaaaaaatac cttctctttt atctgtggtt tcatcggaag 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca 6180

6121 aagattggaa ttgcctggat gtcacgtttt tcaatagttc tctgacgtaa tatctcgcgt 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca 6240

6181 gtttcctctt tttttcatta gattctacga aacaatcttt ttatcgcgag agccctacgt 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg 6300

6241 aaaaacatct tgttttttct tcatatctaa gaaacaacca ttttatcgcg agagcgcaac 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg 6360

6301 gtaaagacaa gacattttta cgtcgagtct aagaaacaaa ctttttaatc gcgagagcgc 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg 6420

6361 aacgtaaaaa caaaatgttt ttacttcgtg tctaagaagc aaccatttta tcgcgaaagc 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc 6480

6421 gcaacgtaaa gacaagacat ttttacgtcg agtctaagaa acaaactttt taatcgcgag 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgtt.... .......... 6526

6481 agcgcaacgt aaaaacaaga tgttttactt cgtgtctacg aagcaa.... .......... 6526

.......... .......... .......... .......... .......... ..........