Sequence of pBudCE4.1

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Sequence of pBudCE4.1

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Sequence of pBudCE4.1 : 4595 BP; 992 A; 1220 C; 1313 G; 1070 T; 0 other;

.......... .......... SpeI(24) .......... .......... ..........

1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 60

1 cgcgcgcaac tgtaactaat aactgatcaa taattatcat tagttaatgc cccagtaatc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 120

61 aagtatcggg tatatacctc aaggcgcaat gtattgaatg ccatttaccg ggcggaccga 120

.......... ....... CMV_immearly_pro ....... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 180

121 ctggcgggtt gctgggggcg ggtaactgca gttattactg catacaaggg tatcattgcg 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 240

181 gttatccctg aaaggtaact gcagttaccc acctgataaa tgccatttga cgggtgaacc 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... NdeI(259) .......... .......... .......... ..........

241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 300

241 gtcatgtagt tcacatagta tacggttcat gcgggggata actgcagtta ctgccattta 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 360

301 ccgggcggac cgtaatacgg gtcatgtact ggaataccct gaaaggatga accgtcatgt 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 420

361 agatgcataa tcagtagcga taatggtacc actacgccaa aaccgtcatg tagttacccg 420

CAG_enh ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 480

421 cacctatcgc caaactgagt gcccctaaag gttcagaggt ggggtaactg cagttaccct 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 540

481 caaacaaaac cgtggtttta gttgccctga aaggttttac agcattgttg aggcggggta 540

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 600

541 actgcgttta cccgccatcc gcacatgcca ccctccagat atattcgtct cgagagaccg 600

...... CMV_fwd_pri CMV_immearly_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 660

601 attgatctct tgggtgacga atgaccgaat agctttaatt atgctgagtg atatccctct 660

....... CMV_immearly_pro ....... .......... T7_pro ..........

HindIII PstI,SalI ClaI,AflII XbaI(707) BamHI(713)

661 cccaagcttg cattcctgca ggtcgacatc gatcttaagc agtacttcta gaggatccga 720

661 gggttcgaac gtaaggacgt ccagctgtag ctagaattcg tcatgaagat ctcctaggct 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc accatcacca 780

721 tgtttttgag tagagtcttc tcctagactt atacgtatgg ccagtagtag tggtagtggt 780

.......... c_myc .......... .......... .......... 6xHis

.......... EcoRI(798) .......... .......... .......... ..........

781 ttgagtttga tccccgggaa ttcagacatg ataagataca ttgatgagtt tggacaaacc 840

781 aactcaaact aggggccctt aagtctgtac tattctatgt aactactcaa acctgtttgg 840

.......... .......... .......... ........ SV40_PA_term EBV_rev_pri

.......... .......... .......... .......... .......... ..........

841 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 900

841 tgttgatctt acgtcacttt ttttacgaaa taaacacttt aaacactacg ataacgaaat 900

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag 960

901 aaacattggt aatattcgac gttatttgtt caaccccacc cgcttcttga ggtcgtactc 960

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct 1020

961 taggggcgcg acctcctagt aggtcggccg cagggccttt tgctaaggct tcgggttgga 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc 1080

1021 aagtatcttc cgccgccacc ttagctttag agcatcgtgc acagtcagga cgaggagccg 1080

.......... .......... .......... .......... ......... sh_ble bleo

.......... .......... .......... .......... .......... ..........

1081 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg 1140

1081 gtgcttcacg tgcgtcaacg gccggcccag cgcgtcccgc ttgagggcgg gggtgccgac 1140

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc 1200

1141 gagcggctag agccagtacc ggccgggcct ccgcagggcc ttcaagcacc tgtgctggag 1200

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc 1260

1201 gctggtgagc cgcatgtcga gcaggtccgg cgcgtgggtg tgggtccggt cccacaacag 1260

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc 1320

1261 gccgtggtgg accaggacct ggcgcgacta cttgtcccag tgcagcaggg cctggtgtgg 1320

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac 1380

1321 ccgcttcagc aggaggtgct tcagggccct cttgggctcg gccagccagg tcttgagctg 1380

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... MscI(1433)

1381 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt 1440

1381 gcgaggccgc tgcagcgcgc gccactcgtg gccttgccgt gaccagttga accggtacca 1440

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc 1500

1441 aatcaaggag tggaacagca taatatgata cggctatatg atacggctac taattaacag 1500

.......... .......... EM7_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag 1560

1501 ttgtgcacga ctagtctagg cttttaccta tatgttcgag ggccctcgaa aaacgttttc 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc 1620

1561 ggatccggag gttttttcgg aggagtgatg aagaccttat cgagtctccg tctccgccgg 1620

.......... .......... .......... .......... SV40_pro ..........

.......... .......... .......... .......... .......... ..........

1621 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg 1680

1621 agccggagac gtatttattt tttttaatca gtcggtaccc cgcctcttac ccgccttgac 1680

SV40pro_F_pri ..... SV40_pro SV40_origin ......... SV40_enh

.......... .......... .......... .......... .......... ..........

1681 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg 1740

1681 ccgcctcaat ccccgcccta cccgcctcaa tccccgccct gataccaacg actgattaac 1740

SV40_origin .... SV40_pro SV40_enh ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt 1800

1741 tctacgtacg aaacgtatga agacggacga cccctcggac ccctgaaagg tgtggaccaa 1800

.......... ...... SV40_pro SV40_enh ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt 1860

1801 cgactgatta actctacgta cgaaacgtat gaagacggac gacccctcgg acccctgaaa 1860

.......... ...... SV40_pro SV40_enh ...... .......... ..........

.......... NheI(1876) .......... .......... .......... ..........

1861 ccacaccctc gtcgagctag cttcgtgagg ctccggtgcc cgtcagtggg cagagcgcac 1920

1861 ggtgtgggag cagctcgatc gaagcactcc gaggccacgg gcagtcaccc gtctcgcgtg 1920

.......... .......... .......... ORF 2 EF1a_pro ..........

.......... .......... .......... .......... .......... ..........

1921 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag 1980

1921 tagcgggtgt caggggctct tcaacccccc tccccagccg ttaacttggc cacggatctc 1980

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga 2040

1981 ttccaccgcg ccccatttga ccctttcact acagcacatg accgaggcgg aaaaagggct 2040

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg 2100

2041 cccaccccct cttggcatat attcacgtca tcagcggcac ttgcaagaaa aagcgttgcc 2100

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac 2160

2101 caaacggcgg tcttgtgtcc attcacggca cacaccaagg gcgcccggac cggagaaatg 2160

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctccagtacg tgattcttga 2220

2161 cccaataccg ggaacgcacg gaacttaatg aaggtggacc gaggtcatgc actaagaact 2220

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcccgagctg gagccagggg cgggccttgc gctttaggag ccccttcgcc tcgtgcttga 2280

2221 agggctcgac ctcggtcccc gcccggaacg cgaaatcctc ggggaagcgg agcacgaact 2280

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc 2340

2281 caactccgga ccggacccgc gaccccggcg gcgcacgctt agaccaccgt ggaagcgcgg 2340

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg 2400

2341 acagagcgac gaaagctatt cagagatcgg taaattttaa aaactactgg acgacgctgc 2400

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctttttttct ggcaagatag tcttgtaaat gcgggccagg atctgcacac tggtatttcg 2460

2401 gaaaaaaaga ccgttctatc agaacattta cgcccggtcc tagacgtgtg accataaagc 2460

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtttttgggc ccgcggccgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg 2520

2461 caaaaacccg ggcgccggcc gctgccccgg gcacgcaggg tcgcgtgtac aagccgctcc 2520

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct 2580

2521 gccccggacg ctcgcgccgg tggctcttag cctgccccca tcagagttcg accggccgga 2580

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc 2640

2581 cgagaccacg gaccggagcg cggcggcaca tagcggggcg ggacccgccg ttccgaccgg 2640

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tccagggggc 2700

2641 gccagccgtg gtcaacgcac tcgcctttct accggcgaag ggccgggacg aggtcccccg 2700

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa 2760

2701 agttttacct cctgcgccgc gagccctctc gcccgcccac tcagtgggtg tgtttccttt 2760

.......... ........ ORF 2 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc 2820

2761 tcccggaaag gcaggagtcg gcagcgaagt acactgaggt gcctcatggc ccgcggcagg 2820

.......... ORF 2 EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aggcacctcg attagttctg gagcttttgg agtacgtcgt ctttaggttg gggggagggg 2880

2821 tccgtggagc taatcaagac ctcgaaaacc tcatgcagca gaaatccaac ccccctcccc 2880

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg 2940

2881 aaaatacgct acctcaaagg ggtgtgactc acccacctct gacttcaatc cggtcgaacc 2940

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cacttgatgt aattctcgtt ggaatttgcc ctttttgagt ttggatcttg gttcattctc 3000

2941 gtgaactaca ttaagagcaa ccttaaacgg gaaaaactca aacctagaac caagtaagag 3000

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aacacgtggt 3060

3001 ttcggagtct gtcaccaagt ttcaaaaaaa gaaggtaaag tccacagcac ttgtgcacca 3060

EF1a_fwd_pri ........ EF1a_pro .......... .......... ..........

NotI(3063) KpnI(3080) .... XhoI(3093) BglII(3099) .... ..........

3061 cgcggccgct tcgaaggtac cagcacagtg gactcgagag atctggccgg ctgggcccgt 3120

3061 gcgccggcga agcttccatg gtcgtgtcac ctgagctctc tagaccggcc gacccgggca 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat 3180

3121 aagcttccat tcggataggg attgggagag gagccagagc taagatgcgc atggccagta 3180

.......... .......... V5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc 3240

3181 gtagtggtag tggtaactca aatttgggcg actagtcgga gctgacacgg aagatcaacg 3240

6xHis .......... .......... ... BGH_rev_pri bGH_PA_term ...

.......... .......... .......... .......... .......... ..........

3241 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 3300

3241 gtcggtagac aacaaacggg gagggggcac ggaaggaact gggaccttcc acggtgaggg 3300

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 3360

3301 tgacaggaaa ggattatttt actcctttaa cgtagcgtaa cagactcatc cacagtaaga 3360

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 3420

3361 taagaccccc caccccaccc cgtcctgtcg ttccccctcc taacccttct gttatcgtcc 3420

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag tggcggtaat 3480

3421 gtacgacccc tacgccaccc gagataccga agactccgcc tttcttggtc accgccatta 3480

......... bGH_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3540

3481 tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3600

3541 tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg 3600

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3660

3601 actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3720

3661 ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3780

3721 cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3840

3781 tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3900

3841 tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3960

3901 ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4020

3961 catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4080

4021 ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 4140

4081 gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 4200

4141 ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgacattaa cctataaaaa 4260

4201 gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactgtaatt ggatattttt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 4320

4261 atccgcatag tgctccggga aagcagagcg cgcaaagcca ctactgccac ttttggagac 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 4380

4321 tgtgtacgtc gagggcctct gccagtgtcg aacagacatt cgcctacggc cctcgtctgt 4380

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 4440

4381 tcgggcagtc ccgcgcagtc gcccacaacc gcccacagcc ccgaccgaat tgatacgccg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc 4500

4441 tagtctcgtc taacatgact ctcacgtggt atatacgcca cactttatgg cgtgtctacg 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... FspI(4546) ..........

4501 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa 4560

4501 cattcctctt ttatggcgta gtccgcggta agcggtaagt ccgacgcgtt gacaaccctt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gggcgatcgg tgcgggcctc ttcgctatta cgcca..... .......... .......... 4595

4561 cccgctagcc acgcccggag aagcgataat gcggt..... .......... .......... 4595

.......... .......... .......... .......... .......... ..........