Sequence of pCAG-CreERT2

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Sequence of pCAG-CreERT2

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Sequence of pCAG-CreERT2 : 6783 BP; 1480 A; 1777 C; 1880 G; 1646 T; 0 other;

SalI(1) SpeI(18) .......... .......... .......... ..........

1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60

1 cagctgtaac taataactga tcaataatta tcattagtta atgccccagt aatcaagtat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120

61 cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga ccgactggcg 120

.......... .......... .......... .......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180

121 ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat tgcggttatc 180

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240

181 cctgaaaggt aactgcagtt acccacctga taaatgccat ttgacgggtg aaccgtcatg 240

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300

241 tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca tttaccgggc 300

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360

301 ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc atgtagatgc 360

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420

361 ataatcagta gcgataatgg tacccagctc cactcggggt gcaagacgaa gtgagagggg 420

CAG_enh .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480

421 tagagggggg ggaggggtgg gggttaaaac ataaataaat aaaaaattaa taaaacacgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 540

481 cgctaccccc gccccccccc cccccgcgcg cggtccgccc cgccccgccc cgctccccgc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600

541 cccgccccgc tccgcctctc cacgccgccg tcggttagtc tcgccgcgcg aggctttcaa 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660

601 aggaaaatac cgctccgccg ccgccgccgc cgggatattt ttcgcttcgc gcgccgcccg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720

661 ccctcagcga cgcaacggaa gcggggcacg gggcgaggcg cggcggagcg cggcgggcgg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780

721 ggccgagact gactggcgca atgagggtgt ccactcgccc gccctgccgg gaagaggagg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840

781 cccgacatta atcgcgaacc aaattactgc cgagcaaaga aaagacaccg acgcactttc 840

.......... .......... .......... .......... ORF 1 ..........

.......... .......... ApaI(863) .......... .......... ..........

841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900

841 ggaatttccc gaggccctcc cgggaaacac gccccccctc gccgagcccc ccacgcacgc 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960

901 acacacacac gcacccctcg cggcgcacgc cgggcgcgac gggccgccga cactcgcgac 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020

961 gcccgcgccg cgccccgaaa cacgcgaggc gcacacgcgc tcccctcgcg ccggcccccg 1020

.......... .......... ORF 1 .......... .......... ..........

SacII(1030) ........ .......... .......... .......... ..........

1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080

1021 ccacggggcg ccacgccccc ccgacgctcc ccttgtttcc gacgcacgcc ccacacacgc 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140

1081 acccccccac tcgtccccca cacccgcgcc gccagcccga cattgggggg ggacgtgggg 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200

1141 ggaggggctc aacgactcgt gccgggccga agcccacgcc ccgaggcacg ccccgcaccg 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260

1201 cgccccgagc ggcacggccc gccccccacc gccgtccacc cccacggccc gccccgcccc 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320

1261 ggcggagccc ggcccctccc gagccccctc cccgcgccgc cggggcctcg cggccgccga 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380

1321 cagctccgcg ccgctcggcg tcggtaacgg aaaataccat tagcacgctc tcccgcgtcc 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1440

1381 ctgaaggaaa cagggtttag accgcctcgg ctttagaccc tccgcggcgg cgtgggggag 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1500

1441 atcgcccgcg cccgcttcgc cacgccgcgg ccgtccttcc tttacccgcc cctcccggaa 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1560

1501 gcacgcagcg gcgcggcggc aggggaagag gtagaggtcg gagccccgac ggcgtccccc 1560

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1620

1561 tgccgacgga agccccccct gccccgtccc gccccaagcc gaagaccgca cactggccgc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1680

1621 cgagatctcg gagacgattg gtacaagtac ggaagaagaa aaaggatgtc gaggacccgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgagcc gccaccatgt 1740

1681 tgcacgacca ataacacgac agagtagtaa aaccgtttct taagactcgg cggtggtaca 1740

pCAG_F_pri .......... .......... .......... .......... ..........

.......... .......... .......... AgeI(1778) .......... ..........

1741 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg 1800

1741 ggttaaatga ctggcatgtg gttttaaacg gacgtaatgg ccagctacgt tgctcactac 1800

.......... .......... ORF 3 CRE_NLS .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct 1860

1801 tccaagcgtt cttggactac ctgtacaagt ccctagcggt ccgcaaaaga ctcgtatgga 1860

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga 1920

1861 ccttttacga agacaggcaa acggccagca cccgccgtac cacgttcaac ttattggcct 1920

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... NruI(1951) .......... ..........

1921 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg 1980

1921 ttaccaaagg gcgtcttgga cttctacaag cgctaataga agatatagaa gtccgcgcgc 1980

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt 2040

1981 cagaccgtca tttttgatag gtcgttgtaa acccggtcga tttgtacgaa gtagcagcca 2040

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa 2100

2041 ggcccgacgg tgctggttca ctgtcgttac gacaaagtga ccaatacgcc gcctaggctt 2100

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... ......... BstBI(2146) .........

2101 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt 2160

2101 ttcttttgca actacggcca cttgcacgtt ttgtccgaga tcgcaagctt gcgtgactaa 2160

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg 2220

2161 agctggtcca agcaagtgag taccttttat cgctagcgac ggtcctatat gcattagacc 2220

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg 2280

2221 gtaaagaccc ctaacgaata ttgtgggaca atgcatatcg gctttaacgg tcctagtccc 2280

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

EcoRV(2288) ........ .......... .......... .......... ..........

2281 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa 2340

2281 aatttctata gagtgcatga ctgccaccct cttacaatta ggtataaccg tcttgctttt 2340

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg 2400

2341 gcgaccaatc gtggcgtcca catctcttcc gtgaatcgga cccccattga tttgaccagc 2400

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg 2460

2401 tcgctaccta aaggcagaga ccacatcgac tactaggctt attgatggac aaaacggccc 2460

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg 2520

2461 agtctttttt accacaacgg cgcggtagac ggtggtcggt cgatagttga gcgcgggacc 2520

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... ClaI(2543) .......... .......... ..........

2521 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga 2580

2521 ttccctaaaa acttcgttga gtagctaact aaatgccgcg attcctactg agaccagtct 2580

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg 2640

2581 ctatggaccg gaccagacct gtgtcacggg cacagcctcg gcgcgctcta taccgggcgc 2640

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca 2700

2641 gacctcaaag ttatggcctc tagtacgttc gaccaccgac ctggttacat ttataacagt 2700

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg 2760

2701 acttgatata ggcattggac ctatcacttt gtccccgtta ccacgcggac gaccttctac 2760

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

XhoI(2766) .......... .......... .......... .......... ..........

2761 gcgatctcga gccatctgct ggagacatga gagctgccaa cctttggcca agcccgctca 2820

2761 cgctagagct cggtagacga cctctgtact ctcgacggtt ggaaaccggt tcgggcgagt 2820

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac cagatggtca 2880

2821 actagtttgc gagattcttc ttgtcggacc ggaacaggga ctgccggctg gtctaccagt 2880

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct accagaccct 2940

2881 cacggaacaa cctacgactc ggggggtatg agataaggct catactagga tggtctggga 2940

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca 3000

2941 agtcacttcg aagctactac ccgaatgact ggttggaccg tctgtccctc gaccaagtgt 3000

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat gatcaggtcc 3060

3001 actagttgac ccgcttctcc cacggtccga aacacctaaa ctgggaggta ctagtccagg 3060

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg cgctccatgg 3120

3061 tggaagatct tacacggacc gatctctagg actactaacc agagcagacc gcgaggtacc 3120

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat 3180

3121 tcgtgggtca cttcgatgac aaacgaggat tgaacgagaa cctgtccttg gtccctttta 3180

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct cggttccgca 3240

3181 cacatctccc gtaccacctc tagaagctgt acgacgaccg atgtagtaga gccaaggcgt 3240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg cttaattctg 3300

3241 actacttaga cgtccctctc ctcaaacaca cggagtttag ataataaaac gaattaagac 3300

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac catatccacc 3360

3301 ctcacatgtg taaagacagg tcgtgggact tcagagacct tctcttcctg gtataggtgg 3360

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca ggcctgaccc 3420

3361 ctcaggacct gttctagtgt ctgtgaaact aggtggacta ccggttccgt ccggactggg 3420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac atcaggcaca 3480

3421 acgtcgtcgt cgtggtcgcc gaccgggtcg aggaggagta ggagagggtg tagtccgtgt 3480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg gtgcccctct 3540

3481 actcattgtt tccgtacctc gtagacatgt cgtacttcac gttcttgcac cacggggaga 3540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact agccgtggag 3600

3541 tactggacga cgacctccgc cgcctgcggg tggcggatgt acgcgggtga tcggcacctc 3600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct acttcatcgc 3660

3601 cccgtaggca cctcctctgc ctggtttcgg tgaaccggtg acgcccgaga tgaagtagcg 3660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc acagcttgat 3720

3661 taaggaacgt tttcataatg tagtgccccc tccgtctccc aaagggacgg tgtcgaacta 3720

.......... .......... ORF 3 .......... .......... ..........

NotI(3723) .......... .......... .......... .......... ..........

3721 agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat 3780

3721 tcgccggcgt gaggagtcca cgtccgacgg atagtcttcc accaccgacc acaccggtta 3780

.......... .......... ....... rb_glob_PA_term ....... ..........

.......... .......... .......... .......... .......... ..........

3781 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc 3840

3781 cgggaccgag tgtttatggt gactctagaa aaagggagac ggtttttaat acccctgtag 3840

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata 3900

3841 tacttcgggg aactcgtaga ctgaagaccg attatttcct ttaaataaaa gtaacgttat 3900

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta 3960

3901 cacacaacct taaaaaacac agagagtgag ccttcctgta taccctcccg tttagtaaat 3960

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca 4020

3961 tttgtagtct tactcataaa ccaaatctca aaccgttgta tacggtatac gaccgacggt 4020

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc 4080

4021 acttgtttcc accgatattt ctccagtagt catatacttt gtcgggggac gacaggtaag 4080

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt 4140

4081 gaataaggta tcttttcgga actgaactcc aatctaaaaa aaatataaaa caaaacacaa 4140

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct 4200

4141 taaaaaaaga aattgtaggg attttaaaag gaatgtacaa aatgatcggt ctaaaaagga 4200

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat ccctcgacct 4260

4201 ggagaggact gatgagggtc agtatcgaca gggagaagag aatacttcta gggagctgga 4260

....... rb_glob_PA_term ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 4320

4261 cgtcgggttc gaaccgcatt agtaccagta tcgacaaagg acacacttta acaataggcg 4320

.......... .......... .......... M13_r M13_pUC_r ..........

.......... .......... .......... .......... .......... ..........

4321 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 4380

4321 agtgttaagg tgtgttgtat gctcggcctt cgtatttcac atttcggacc ccacggatta 4380

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 4440

4381 ctcactcgat tgagtgtaat taacgcaacg cgagtgacgg gcgaaaggtc agccctttgg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 4500

4441 acagcacggt cgcctaggcg tagagttaat cagtcgttgg tatcagggcg gggattgagg 4500

.......... .......... .......... .......... SV40_pro ..........

.......... .......... .......... .......... .......... ..........

4501 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 4560

4501 cgggtagggc ggggattgag gcgggtcaag gcgggtaaga ggcggggtac cgactgatta 4560

.......... ......... SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

4561 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 4620

4561 aaaaaaataa atacgtctcc ggctccggcg gagccggaga ctcgataagg tcttcatcac 4620

.... SV40_origin SV40pro_F SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat 4680

4621 tcctccgaaa aaacctccgg atccgaaaac gtttttcgat tgaacaaata acgtcgaata 4680

.......... SV40_pro .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

4681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 4740

4681 ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt tatttcgtaa aaaaagtgac 4740

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc 4800

4741 gtaagatcaa caccaaacag gtttgagtag ttacatagaa tagtacagac ctaggcgacg 4800

......... EBV_r SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 4860

4801 taattactta gccggttgcg cgcccctctc cgccaaacgc ataacccgcg agaaggcgaa 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4920

4861 ggagcgagtg actgagcgac gcgagccagc aagccgacgc cgctcgccat agtcgagtga 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4980

4921 gtttccgcca ttatgccaat aggtgtctta gtcccctatt gcgtcctttc ttgtacactc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5040

4981 gttttccggt cgttttccgg tccttggcat ttttccggcg caacgaccgc aaaaaggtat 5040

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5100

5041 ccgaggcggg gggactgctc gtagtgtttt tagctgcgag ttcagtctcc accgctttgg 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5160

5101 gctgtcctga tatttctatg gtccgcaaag ggggaccttc gagggagcac gcgagaggac 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5220

5161 aaggctggga cggcgaatgg cctatggaca ggcggaaaga gggaagccct tcgcaccgcg 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5280

5221 aaagagttac gagtgcgaca tccatagagt caagccacat ccagcaagcg aggttcgacc 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5340

5281 cgacacacgt gcttgggggg caagtcgggc tggcgacgcg gaataggcca ttgatagcag 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5400

5341 aactcaggtt gggccattct gtgctgaata gcggtgaccg tcgtcggtga ccattgtcct 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5460

5401 aatcgtctcg ctccatacat ccgccacgat gtctcaagaa cttcaccacc ggattgatgc 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5520

5461 cgatgtgatc ttcctgtcat aaaccataga cgcgagacga cttcggtcaa tggaagcctt 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 5580

5521 tttctcaacc atcgagaact aggccgtttg tttggtggcg accatcgcca ccaaaaaaac 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 5640

5581 aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt tcttctagga aactagaaaa 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 5700

5641 gatgccccag actgcgagtc accttgcttt tgagtgcaat tccctaaaac cagtactcta 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 5760

5701 atagtttttc ctagaagtgg atctaggaaa atttaatttt tacttcaaaa tttagttaga 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 5820

5761 tttcatatat actcatttga accagactgt caatggttac gaattagtca ctccgtggat 5820

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

5821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 5880

5821 agagtcgcta gacagataaa gcaagtaggt atcaacggac tgaggggcag cacatctatt 5880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 5940

5881 gatgctatgc cctcccgaat ggtagaccgg ggtcacgacg ttactatggc gctctgggtg 5940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 6000

5941 cgagtggccg aggtctaaat agtcgttatt tggtcggtcg gccttcccgg ctcgcgtctt 6000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 6060

6001 caccaggacg ttgaaatagg cggaggtagg tcagataatt aacaacggcc cttcgatctc 6060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... FspI(6087) .......... .......... ..........

6061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 6120

6061 attcatcaag cggtcaatta tcaaacgcgt tgcaacaacg gtaacgatgt ccgtagcacc 6120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 6180

6121 acagtgcgag cagcaaacca taccgaagta agtcgaggcc aagggttgct agttccgctc 6180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 6240

6181 aatgtactag ggggtacaac acgttttttc gccaatcgag gaagccagga ggctagcaac 6240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 6300

6241 agtcttcatt caaccggcgt cacaatagtg agtaccaata ccgtcgtgac gtattaagag 6300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 6360

6301 aatgacagta cggtaggcat tctacgaaaa gacactgacc actcatgagt tggttcagta 6360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 6420

6361 agactcttat cacatacgcc gctggctcaa cgagaacggg ccgcagttat gccctattat 6420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 6480

6421 ggcgcggtgt atcgtcttga aattttcacg agtagtaacc ttttgcaaga agccccgctt 6480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 6540

6481 ttgagagttc ctagaatggc gacaactcta ggtcaagcta cattgggtga gcacgtgggt 6540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 6600

6541 tgactagaag tcgtagaaaa tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg 6600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 6660

6601 ttttacggcg ttttttccct tattcccgct gtgcctttac aacttatgag tatgagaagg 6660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 6720

6661 aaaaagttat aataacttcg taaatagtcc caataacaga gtactcgcct atgtataaac 6720

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

6721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 6780

6721 ttacataaat ctttttattt gtttatcccc aaggcgcgtg taaaggggct tttcacggtg 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ctg....... .......... .......... .......... .......... .......... 6783

6781 gac....... .......... .......... .......... .......... .......... 6783

.......... .......... .......... .......... .......... ..........