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    Plasmid map of pCAG-Cre_GFP

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    Gene/insert name: Cre-GFP fusion protein
    Alternative names: Cre recombinase
    Insert size (bp): 1784
    Species of gene(s): Bacteriophage P1
    Fusion proteins or tags: GFP
    Terminal: C terminal on insert
    Vector backbone: pCAGEN (Search Vector Database)
    Type of vector: Mammalian expression
    Backbone size (bp): 6563
    Cloning site 5': EcoRI
    Site destroyed during cloning: No
    Cloning site 3': NotI
    Site destroyed during cloning: No
    5' Sequencing primer: pCAG-F (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template. GFP was from pEGFP-N1 (Clontech).
    Plasmid Provided In: DH5a
    Principal Investigator: Connie Cepko
    Comments: Kozak consensus sequence was added before the start ATG. GFP-tag was added at the C-terminus of Cre. Cre-GFP fusion protein is localized in the cell nucleus.
    Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)

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