Sequence of pCAG-Cre

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Sequence of pCAG-Cre_GFP

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Sequence of pCAG-Cre_GFP : 5551 BP; 1181 A; 1524 C; 1557 G; 1289 T; 0 other;

SalI(1) SpeI(18) .......... .......... .......... ..........

1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60

1 cagctgtaac taataactga tcaataatta tcattagtta atgccccagt aatcaagtat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120

61 cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga ccgactggcg 120

.......... .......... .......... .......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180

121 ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat tgcggttatc 180

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240

181 cctgaaaggt aactgcagtt acccacctga taaatgccat ttgacgggtg aaccgtcatg 240

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300

241 tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca tttaccgggc 300

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360

301 ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc atgtagatgc 360

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420

361 ataatcagta gcgataatgg tacccagctc cactcggggt gcaagacgaa gtgagagggg 420

CAG_enh .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480

421 tagagggggg ggaggggtgg gggttaaaac ataaataaat aaaaaattaa taaaacacgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 540

481 cgctaccccc gccccccccc cccccgcgcg cggtccgccc cgccccgccc cgctccccgc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600

541 cccgccccgc tccgcctctc cacgccgccg tcggttagtc tcgccgcgcg aggctttcaa 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660

601 aggaaaatac cgctccgccg ccgccgccgc cgggatattt ttcgcttcgc gcgccgcccg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720

661 ccctcagcga cgcaacggaa gcggggcacg gggcgaggcg cggcggagcg cggcgggcgg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780

721 ggccgagact gactggcgca atgagggtgt ccactcgccc gccctgccgg gaagaggagg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840

781 cccgacatta atcgcgaacc aaattactgc cgagcaaaga aaagacaccg acgcactttc 840

.......... .......... .......... .......... ORF 1 ..........

.......... .......... ApaI(863) .......... .......... ..........

841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900

841 ggaatttccc gaggccctcc cgggaaacac gccccccctc gccgagcccc ccacgcacgc 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960

901 acacacacac gcacccctcg cggcgcacgc cgggcgcgac gggccgccga cactcgcgac 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020

961 gcccgcgccg cgccccgaaa cacgcgaggc gcacacgcgc tcccctcgcg ccggcccccg 1020

.......... .......... ORF 1 .......... .......... ..........

SacII(1030) ........ .......... .......... .......... ..........

1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080

1021 ccacggggcg ccacgccccc ccgacgctcc ccttgtttcc gacgcacgcc ccacacacgc 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140

1081 acccccccac tcgtccccca cacccgcgcc gccagcccga cattgggggg ggacgtgggg 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200

1141 ggaggggctc aacgactcgt gccgggccga agcccacgcc ccgaggcacg ccccgcaccg 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260

1201 cgccccgagc ggcacggccc gccccccacc gccgtccacc cccacggccc gccccgcccc 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320

1261 ggcggagccc ggcccctccc gagccccctc cccgcgccgc cggggcctcg cggccgccga 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380

1321 cagctccgcg ccgctcggcg tcggtaacgg aaaataccat tagcacgctc tcccgcgtcc 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1440

1381 ctgaaggaaa cagggtttag accgcctcgg ctttagaccc tccgcggcgg cgtgggggag 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1500

1441 atcgcccgcg cccgcttcgc cacgccgcgg ccgtccttcc tttacccgcc cctcccggaa 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1560

1501 gcacgcagcg gcgcggcggc aggggaagag gtagaggtcg gagccccgac ggcgtccccc 1560

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1620

1561 tgccgacgga agccccccct gccccgtccc gccccaagcc gaagaccgca cactggccgc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1680

1621 cgagatctcg gagacgattg gtacaagtac ggaagaagaa aaaggatgtc gaggacccgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(1719) ......... ..........

1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc 1740

1681 tgcacgacca ataacacgac agagtagtaa aaccgtttct taagacgtca gctgccatgg 1740

pCAG_F_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 1800

1741 cgcccgggcc ctaggtggcc agcggtggta ccactcgttc ccgctcctcg acaagtggcc 1800

.......... .......... ORF 3 CRE_NLS .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 1860

1801 ccaccacggg taggaccagc tcgacctgcc gctgcatttg ccggtgttca agtcgcacag 1860

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 1920

1861 gccgctcccg ctcccgctac ggtggatgcc gttcgactgg gacttcaagt agacgtggtg 1920

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... NruI(1951) .......... ..........

1921 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg 1980

1921 gccgttcgac gggcacggga ccgggtggga gcactggtgg gactggatgc cgcacgtcac 1980

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 2040

1981 gaagtcggcg atggggctgg tgtacttcgt cgtgctgaag aagttcaggc ggtacgggct 2040

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 2100

2041 tccgatgcag gtcctcgcgt ggtagaagaa gttcctgctg ccgttgatgt tctgggcgcg 2100

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 2160

2101 gctccacttc aagctcccgc tgtgggacca cttggcgtag ctcgacttcc cgtagctgaa 2160

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 2220

2161 gttcctcctg ccgttgtagg accccgtgtt cgacctcatg ttgatgttgt cggtgttgca 2220

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 2280

2221 gatatagtac cggctgttcg tcttcttgcc gtagttccac ttgaagttct aggcggtgtt 2280

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

EcoRV(2288) ........ .......... .......... .......... ..........

2281 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 2340

2281 gtagctcctg ccgtcgcacg tcgagcggct ggtgatggtc gtcttgtggg ggtagccgct 2340

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 2400

2341 gccggggcac gacgacgggc tgttggtgat ggactcgtgg gtcaggcggg actcgtttct 2400

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 2460

2401 ggggttgctc ttcgcgctag tgtaccagga cgacctcaag cactggcggc ggccctagtg 2460

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tctcggcatg gacgagctgt acaagtaaag cggccgcact cctcaggtgc aggctgccta 2520

2461 agagccgtac ctgctcgaca tgttcatttc gccggcgtga ggagtccacg tccgacggat 2520

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... ClaI(2543) .......... .......... ..........

2521 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt 2580

2521 agtcttccac caccgaccac accggttacg ggaccgagtg tttatggtga ctctagaaaa 2580

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta 2640

2581 agggagacgg tttttaatac ccctgtagta cttcggggaa ctcgtagact gaagaccgat 2640

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 2700

2641 tatttccttt aaataaaagt aacgttatca cacaacctta aaaaacacag agagtgagcc 2700

.......... ........ ORF 3 CRE_NLS ORF 3 ........ ..........

.......... .......... .......... .......... .......... ..........

2701 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt 2760

2701 ttcctgtata ccctcccgtt tagtaaattt tgtagtctta ctcataaacc aaatctcaaa 2760

.......... ..... ORF 3 CRE_NLS ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt 2820

2761 ccgttgtata cggtatacga ccgacggtac ttgtttccac cgatatttct ccagtagtca 2820

.......... ......... ORF 3 ORF 3 EGFP ......... ..........

.......... .......... .......... .......... .......... ..........

2821 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt 2880

2821 tatactttgt cgggggacga caggtaagga ataaggtatc ttttcggaac tgaactccaa 2880

....... EGFP_N_pri ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct 2940

2881 tctaaaaaaa atataaaaca aaacacaata aaaaaagaaa ttgtagggat tttaaaagga 2940

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc 3000

2941 atgtacaaaa tgatcggtct aaaaaggagg agaggactga tgagggtcag tatcgacagg 3000

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctcttctctt atgaagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag 3060

3001 gagaagagaa tacttctagg gagctggacg tcgggttcga accgcattag taccagtatc 3060

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 3120

3061 gacaaaggac acactttaac aataggcgag tgttaaggtg tgttgtatgc tcggccttcg 3120

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 3180

3121 tatttcacat ttcggacccc acggattact cactcgattg agtgtaatta acgcaacgcg 3180

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt 3240

3181 agtgacgggc gaaaggtcag ccctttggac agcacggtcg cctaggcgta gagttaatca 3240

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 3300

3241 gtcgttggta tcagggcggg gattgaggcg ggtagggcgg ggattgaggc gggtcaaggc 3300

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 3360

3301 gggtaagagg cggggtaccg actgattaaa aaaaataaat acgtctccgg ctccggcgga 3360

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 3420

3361 gccggagact cgataaggtc ttcatcactc ctccgaaaaa acctccggat ccgaaaacgt 3420

.......... ....... ORF 3 ORF 3 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 3480

3421 ttttcgattg aacaaataac gtcgaatatt accaatgttt atttcgttat cgtagtgttt 3480

.......... . ORF 3 ORF 3 EGFP EGFP_C_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 3540

3481 aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt 3540

ORF 3 EGFP .......... ORF 3 ....... rb_glob_PA_term .......

.......... .......... .......... ......... BglII(3584) .........

3541 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc 3600

3541 acatagaata gtacagacct aggcgacgta attacttagc cggttgcgcg cccctctccg 3600

.......... .... ORF 3 rb_glob_PA_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 3660

3601 ccaaacgcat aacccgcgag aaggcgaagg agcgagtgac tgagcgacgc gagccagcaa 3660

....... ORF 3 rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 3720

3661 gccgacgccg ctcgccatag tcgagtgagt ttccgccatt atgccaatag gtgtcttagt 3720

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 3780

3721 cccctattgc gtcctttctt gtacactcgt tttccggtcg ttttccggtc cttggcattt 3780

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 3840

3781 ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg gactgctcgt agtgttttta 3840

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 3900

3841 gctgcgagtt cagtctccac cgctttgggc tgtcctgata tttctatggt ccgcaaaggg 3900

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 3960

3901 ggaccttcga gggagcacgc gagaggacaa ggctgggacg gcgaatggcc tatggacagg 3960

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 4020

3961 cggaaagagg gaagcccttc gcaccgcgaa agagttacga gtgcgacatc catagagtca 4020

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... ... PstI(4043),HindIII(4048) ... .......... ..........

4021 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4080

4021 agccacatcc agcaagcgag gttcgacccg acacacgtgc ttggggggca agtcgggctg 4080

.......... .......... .......... .......... .......... M13_r

.......... .......... .......... .......... .......... ..........

4081 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4140

4081 gcgacgcgga ataggccatt gatagcagaa ctcaggttgg gccattctgt gctgaatagc 4140

.......... M13_pUC_r .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

4141 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4200

4141 ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct ccatacatcc gccacgatgt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 4260

4201 ctcaagaact tcaccaccgg attgatgccg atgtgatctt cctgtcataa accatagacg 4260

.......... .......... .......... .......... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

4261 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4320

4261 cgagacgact tcggtcaatg gaagcctttt tctcaaccat cgagaactag gccgtttgtt 4320

.......... .......... SV40_pro .......... ......... SV40_origin

.......... .......... .......... .......... .......... ..........

4321 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 4380

4321 tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg tctaatgcgc gtcttttttt 4380

.......... ... SV40_pro SV40_origin SV40pro_F_pri ... ..........

.......... .......... .......... StuI(4418) .......... ..........

4381 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 4440

4381 cctagagttc ttctaggaaa ctagaaaaga tgccccagac tgcgagtcac cttgcttttg 4440

.......... .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 4500

4441 agtgcaattc cctaaaacca gtactctaat agtttttcct agaagtggat ctaggaaaat 4500

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

4501 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 4560

4501 ttaattttta cttcaaaatt tagttagatt tcatatatac tcatttgaac cagactgtca 4560

.......... ........ SV40_PA_term EBV_rev_pri ........ ..........

.......... .......... .......... .......... .......... ..........

4561 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 4620

4561 atggttacga attagtcact ccgtggatag agtcgctaga cagataaagc aagtaggtat 4620

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 4680

4621 caacggactg aggggcagca catctattga tgctatgccc tcccgaatgg tagaccgggg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 4740

4681 tcacgacgtt actatggcgc tctgggtgcg agtggccgag gtctaaatag tcgttatttg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 4800

4741 gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt gaaataggcg gaggtaggtc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 4860

4801 agataattaa caacggccct tcgatctcat tcatcaagcg gtcaattatc aaacgcgttg 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 4920

4861 caacaacggt aacgatgtcc gtagcaccac agtgcgagca gcaaaccata ccgaagtaag 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 4980

4921 tcgaggccaa gggttgctag ttccgctcaa tgtactaggg ggtacaacac gttttttcgc 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 5040

4981 caatcgagga agccaggagg ctagcaacag tcttcattca accggcgtca caatagtgag 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 5100

5041 taccaatacc gtcgtgacgt attaagagaa tgacagtacg gtaggcattc tacgaaaaga 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 5160

5101 cactgaccac tcatgagttg gttcagtaag actcttatca catacgccgc tggctcaacg 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 5220

5161 agaacgggcc gcagttatgc cctattatgg cgcggtgtat cgtcttgaaa ttttcacgag 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 5280

5221 tagtaacctt ttgcaagaag ccccgctttt gagagttcct agaatggcga caactctagg 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 5340

5281 tcaagctaca ttgggtgagc acgtgggttg actagaagtc gtagaaaatg aaagtggtcg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 5400

5341 caaagaccca ctcgtttttg tccttccgtt ttacggcgtt ttttccctta ttcccgctgt 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 5460

5401 gcctttacaa cttatgagta tgagaaggaa aaagttataa taacttcgta aatagtccca 5460

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 5520

5461 ataacagagt actcgcctat gtataaactt acataaatct ttttatttgt ttatccccaa 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ccgcgcacat ttccccgaaa agtgccacct g......... .......... .......... 5551

5521 ggcgcgtgta aaggggcttt tcacggtgga c......... .......... .......... 5551

.......... .......... .......... .......... ....... ORF 1 Ampicillin