Sequence of pCAG-Cre

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Sequence of pCAG-Cre

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Sequence of pCAG-Cre : 5871 BP; 1277 A; 1515 C; 1637 G; 1442 T; 0 other;

SalI(1) SpeI(18) .......... .......... .......... ..........

1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60

1 cagctgtaac taataactga tcaataatta tcattagtta atgccccagt aatcaagtat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120

61 cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga ccgactggcg 120

.......... .......... .......... .......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180

121 ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat tgcggttatc 180

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240

181 cctgaaaggt aactgcagtt acccacctga taaatgccat ttgacgggtg aaccgtcatg 240

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300

241 tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca tttaccgggc 300

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360

301 ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc atgtagatgc 360

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420

361 ataatcagta gcgataatgg tacccagctc cactcggggt gcaagacgaa gtgagagggg 420

CAG_enh .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480

421 tagagggggg ggaggggtgg gggttaaaac ataaataaat aaaaaattaa taaaacacgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 540

481 cgctaccccc gccccccccc cccccgcgcg cggtccgccc cgccccgccc cgctccccgc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600

541 cccgccccgc tccgcctctc cacgccgccg tcggttagtc tcgccgcgcg aggctttcaa 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660

601 aggaaaatac cgctccgccg ccgccgccgc cgggatattt ttcgcttcgc gcgccgcccg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720

661 ccctcagcga cgcaacggaa gcggggcacg gggcgaggcg cggcggagcg cggcgggcgg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780

721 ggccgagact gactggcgca atgagggtgt ccactcgccc gccctgccgg gaagaggagg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840

781 cccgacatta atcgcgaacc aaattactgc cgagcaaaga aaagacaccg acgcactttc 840

.......... .......... .......... .......... ORF 1 ..........

.......... .......... ApaI(863) .......... .......... ..........

841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900

841 ggaatttccc gaggccctcc cgggaaacac gccccccctc gccgagcccc ccacgcacgc 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960

901 acacacacac gcacccctcg cggcgcacgc cgggcgcgac gggccgccga cactcgcgac 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020

961 gcccgcgccg cgccccgaaa cacgcgaggc gcacacgcgc tcccctcgcg ccggcccccg 1020

.......... .......... ORF 1 .......... .......... ..........

SacII(1030) ........ .......... .......... .......... ..........

1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080

1021 ccacggggcg ccacgccccc ccgacgctcc ccttgtttcc gacgcacgcc ccacacacgc 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140

1081 acccccccac tcgtccccca cacccgcgcc gccagcccga cattgggggg ggacgtgggg 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200

1141 ggaggggctc aacgactcgt gccgggccga agcccacgcc ccgaggcacg ccccgcaccg 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260

1201 cgccccgagc ggcacggccc gccccccacc gccgtccacc cccacggccc gccccgcccc 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320

1261 ggcggagccc ggcccctccc gagccccctc cccgcgccgc cggggcctcg cggccgccga 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380

1321 cagctccgcg ccgctcggcg tcggtaacgg aaaataccat tagcacgctc tcccgcgtcc 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1440

1381 ctgaaggaaa cagggtttag accgcctcgg ctttagaccc tccgcggcgg cgtgggggag 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1500

1441 atcgcccgcg cccgcttcgc cacgccgcgg ccgtccttcc tttacccgcc cctcccggaa 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1560

1501 gcacgcagcg gcgcggcggc aggggaagag gtagaggtcg gagccccgac ggcgtccccc 1560

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1620

1561 tgccgacgga agccccccct gccccgtccc gccccaagcc gaagaccgca cactggccgc 1620

.......... .......... .......... .......... .......... ..........

XbaI(1623) .......... .......... .......... .......... ..........

1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1680

1621 cgagatctcg gagacgattg gtacaagtac ggaagaagaa aaaggatgtc gaggacccgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(1719) ......... ..........

1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgagcc gccaccatgg 1740

1681 tgcacgacca ataacacgac agagtagtaa aaccgtttct taagactcgg cggtggtacc 1740

pCAG_F_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg 1800

1741 ggttaaatga ctggcatgtg gttttaaacg gacgtaatgg ccagctacgt tgctcactac 1800

.......... .......... ORF 3 CRE_NLS .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct 1860

1801 tccaagcgtt cttggactac ctgtacaagt ccctagcggt ccgcaaaaga ctcgtatgga 1860

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga 1920

1861 ccttttacga agacaggcaa acggccagca cccgccgtac cacgttcaac ttattggcct 1920

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... NruI(1951) .......... ..........

1921 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg 1980

1921 ttaccaaagg gcgtcttgga cttctacaag cgctaataga agatatagaa gtccgcgcgc 1980

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt 2040

1981 cagaccgtca tttttgatag gtcgttgtaa acccggtcga tttgtacgaa gtagcagcca 2040

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa 2100

2041 ggcccgacgg tgctggttca ctgtcgttac gacaaagtga ccaatacgcc gcctaggctt 2100

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... ......... BstBI(2146) .........

2101 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt 2160

2101 ttcttttgca actacggcca cttgcacgtt ttgtccgaga tcgcaagctt gcgtgactaa 2160

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg 2220

2161 agctggtcca agcaagtgag taccttttat cgctagcgac ggtcctatat gcattagacc 2220

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg 2280

2221 gtaaagaccc ctaacgaata ttgtgggaca atgcatatcg gctttaacgg tcctagtccc 2280

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

EcoRV(2288) ........ .......... .......... .......... ..........

2281 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa 2340

2281 aatttctata gagtgcatga ctgccaccct cttacaatta ggtataaccg tcttgctttt 2340

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg 2400

2341 gcgaccaatc gtggcgtcca catctcttcc gtgaatcgga cccccattga tttgaccagc 2400

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg 2460

2401 tcgctaccta aaggcagaga ccacatcgac tactaggctt attgatggac aaaacggccc 2460

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg 2520

2461 agtctttttt accacaacgg cgcggtagac ggtggtcggt cgatagttga gcgcgggacc 2520

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... ClaI(2543) .......... .......... ..........

2521 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga 2580

2521 ttccctaaaa acttcgttga gtagctaact aaatgccgcg attcctactg agaccagtct 2580

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg 2640

2581 ctatggaccg gaccagacct gtgtcacggg cacagcctcg gcgcgctcta taccgggcgc 2640

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca 2700

2641 gacctcaaag ttatggcctc tagtacgttc gaccaccgac ctggttacat ttataacagt 2700

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg 2760

2701 acttgatata ggcattggac ctatcacttt gtccccgtta ccacgcggac gaccttctac 2760

.......... ........ ORF 3 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... NotI(2811)

2761 gcgatggacc ggtggaacaa aaacttattt ctgaagaaga tctgtgatag cggccgcact 2820

2761 cgctacctgg ccaccttgtt tttgaataaa gacttcttct agacactatc gccggcgtga 2820

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac 2880

2821 ggagtccacg tccgacggat agtcttccac caccgaccac accggttacg ggaccgagtg 2880

.......... .......... ....... rb_glob_PA_term ....... ..........

.......... .......... .......... .......... .......... ..........

2881 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 2940

2881 tttatggtga ctctagaaaa agggagacgg tttttaatac ccctgtagta cttcggggaa 2940

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 3000

2941 ctcgtagact gaagaccgat tatttccttt aaataaaagt aacgttatca cacaacctta 3000

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 3060

3001 aaaaacacag agagtgagcc ttcctgtata ccctcccgtt tagtaaattt tgtagtctta 3060

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg aacaaaggtg 3120

3061 ctcataaacc aaatctcaaa ccgttgtata cggtatacga ccgacggtac ttgtttccac 3120

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 3180

3121 cgatatttct ccagtagtca tatactttgt cgggggacga caggtaagga ataaggtatc 3180

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 3240

3181 ttttcggaac tgaactccaa tctaaaaaaa atataaaaca aaacacaata aaaaaagaaa 3240

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 3300

3241 ttgtagggat tttaaaagga atgtacaaaa tgatcggtct aaaaaggagg agaggactga 3300

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... ... PstI(3351),HindIII(3356)

3301 actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc agcccaagct 3360

3301 tgagggtcag tatcgacagg gagaagagaa tacttctagg gagctggacg tcgggttcga 3360

....... rb_glob_PA_term ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 3420

3361 accgcattag taccagtatc gacaaaggac acactttaac aataggcgag tgttaaggtg 3420

.......... M13_r .......... M13_pUC_r .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 3480

3421 tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact cactcgattg 3480

.......... lac .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 3540

3481 agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac agcacggtcg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 3600

3541 cctaggcgta gagttaatca gtcgttggta tcagggcggg gattgaggcg ggtagggcgg 3600

.......... .......... .......... SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 3660

3601 ggattgaggc gggtcaaggc gggtaagagg cggggtaccg actgattaaa aaaaataaat 3660

.......... ..... SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 3720

3661 acgtctccgg ctccggcgga gccggagact cgataaggtc ttcatcactc ctccgaaaaa 3720

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

StuI(3726) .......... .......... .......... .......... ..........

3721 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa 3780

3721 acctccggat ccgaaaacgt ttttcgattg aacaaataac gtcgaatatt accaatgttt 3780

SV40_pro .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

3781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 3840

3781 atttcgttat cgtagtgttt aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca 3840

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg 3900

3841 ccaaacaggt ttgagtagtt acatagaata gtacagacct aggcgacgta attacttagc 3900

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 3960

3901 cggttgcgcg cccctctccg ccaaacgcat aacccgcgag aaggcgaagg agcgagtgac 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 4020

3961 tgagcgacgc gagccagcaa gccgacgccg ctcgccatag tcgagtgagt ttccgccatt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 4080

4021 atgccaatag gtgtcttagt cccctattgc gtcctttctt gtacactcgt tttccggtcg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 4140

4081 ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg 4140

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 4200

4141 gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc tgtcctgata 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 4260

4201 tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa ggctgggacg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 4320

4261 gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa agagttacga 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 4380

4321 gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg acacacgtgc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 4440

4381 ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa ctcaggttgg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 4500

4441 gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 4560

4501 ccatacatcc gccacgatgt ctcaagaact tcaccaccgg attgatgccg atgtgatctt 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 4620

4561 cctgtcataa accatagacg cgagacgact tcggtcaatg gaagcctttt tctcaaccat 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 4680

4621 cgagaactag gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 4740

4681 tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga tgccccagac 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 4800

4741 tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtactctaat agtttttcct 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 4860

4801 agaagtggat ctaggaaaat ttaattttta cttcaaaatt tagttagatt tcatatatac 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 4920

4861 tcatttgaac cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga 4920

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4980

4921 cagataaagc aagtaggtat caacggactg aggggcagca catctattga tgctatgccc 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5040

4981 tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 5100

5041 gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 5160

5101 gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... FspI(5175) .......... .......... .......... ..........

5161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 5220

5161 gtcaattatc aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 5280

5221 gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 5340

5281 ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 5400

5341 accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg 5400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 5460

5401 gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca 5460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 5520

5461 catacgccgc tggctcaacg agaacgggcc gcagttatgc cctattatgg cgcggtgtat 5520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 5580

5521 cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct 5580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 5640

5581 agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc 5640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 5700

5641 gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt 5700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 5760

5701 ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa 5760

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 5820

5761 taacttcgta aatagtccca ataacagagt actcgcctat gtataaactt acataaatct 5820

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g......... 5871

5821 ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga c......... 5871

.......... .......... .......... .......... .......... ..........