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pCAG-ERT2CreERT2
Gene/insert name: ERT2-Cre-ERT2 fusion protein
Alternative names: inducible Cre
Insert size (bp): 2951
Species of gene(s): Bacteriophage P1
Vector backbone: pCAGEN (Search Vector Database)
Type of vector: Mammalian expression,Cre/Lox
Backbone size (bp): 7730
Cloning site 5': EcoRI
Site destroyed during cloning: No
Cloning site 3': NotI
Site destroyed during cloning: No
5' Sequencing primer: pCAG-F (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: ERT2CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
Plasmid Provided In: DH5a
Principal Investigator: Connie Cepko
Comments: 4-hydroxitamoxifen-responsible Cre. Kozak consensus sequence was added before the start ATG. The insert can be cut out with EcoRI (SmaI) and NotI.
Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)