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    Plasmid map of pCAG-ERT2CreERT2

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    Gene/insert name: ERT2-Cre-ERT2 fusion protein
    Alternative names: inducible Cre
    Insert size (bp): 2951
    Species of gene(s): Bacteriophage P1
    Vector backbone: pCAGEN (Search Vector Database)
    Type of vector: Mammalian expression,Cre/Lox
    Backbone size (bp): 7730
    Cloning site 5': EcoRI
    Site destroyed during cloning: No
    Cloning site 3': NotI
    Site destroyed during cloning: No
    5' Sequencing primer: pCAG-F (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: ERT2CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
    Plasmid Provided In: DH5a
    Principal Investigator: Connie Cepko
    Comments: 4-hydroxitamoxifen-responsible Cre. Kozak consensus sequence was added before the start ATG. The insert can be cut out with EcoRI (SmaI) and NotI.
    Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)

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