Sequence of pCAG-ERT2CreERT2

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Sequence of pCAG-ERT2CreERT2

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Sequence of pCAG-ERT2CreERT2 : 7730 BP; 1696 A; 2042 C; 2137 G; 1855 T; 0 other;

.......... SpeI(18) .......... .......... .......... ..........

1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60

1 cagctgtaac taataactga tcaataatta tcattagtta atgccccagt aatcaagtat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120

61 cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga ccgactggcg 120

.......... .......... .......... .......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180

121 ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat tgcggttatc 180

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240

181 cctgaaaggt aactgcagtt acccacctga taaatgccat ttgacgggtg aaccgtcatg 240

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300

241 tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca tttaccgggc 300

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360

301 ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc atgtagatgc 360

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420

361 ataatcagta gcgataatgg tacccagctc cactcggggt gcaagacgaa gtgagagggg 420

CAG_enh .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480

421 tagagggggg ggaggggtgg gggttaaaac ataaataaat aaaaaattaa taaaacacgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 540

481 cgctaccccc gccccccccc cccccgcgcg cggtccgccc cgccccgccc cgctccccgc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600

541 cccgccccgc tccgcctctc cacgccgccg tcggttagtc tcgccgcgcg aggctttcaa 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660

601 aggaaaatac cgctccgccg ccgccgccgc cgggatattt ttcgcttcgc gcgccgcccg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720

661 ccctcagcga cgcaacggaa gcggggcacg gggcgaggcg cggcggagcg cggcgggcgg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780

721 ggccgagact gactggcgca atgagggtgt ccactcgccc gccctgccgg gaagaggagg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840

781 cccgacatta atcgcgaacc aaattactgc cgagcaaaga aaagacaccg acgcactttc 840

.......... .......... .......... .......... ORF 1 ..........

.......... .......... ApaI(863) .......... .......... ..........

841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900

841 ggaatttccc gaggccctcc cgggaaacac gccccccctc gccgagcccc ccacgcacgc 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960

901 acacacacac gcacccctcg cggcgcacgc cgggcgcgac gggccgccga cactcgcgac 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020

961 gcccgcgccg cgccccgaaa cacgcgaggc gcacacgcgc tcccctcgcg ccggcccccg 1020

.......... .......... ORF 1 .......... .......... ..........

SacII(1030) ........ .......... .......... .......... ..........

1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080

1021 ccacggggcg ccacgccccc ccgacgctcc ccttgtttcc gacgcacgcc ccacacacgc 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140

1081 acccccccac tcgtccccca cacccgcgcc gccagcccga cattgggggg ggacgtgggg 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200

1141 ggaggggctc aacgactcgt gccgggccga agcccacgcc ccgaggcacg ccccgcaccg 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260

1201 cgccccgagc ggcacggccc gccccccacc gccgtccacc cccacggccc gccccgcccc 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320

1261 ggcggagccc ggcccctccc gagccccctc cccgcgccgc cggggcctcg cggccgccga 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380

1321 cagctccgcg ccgctcggcg tcggtaacgg aaaataccat tagcacgctc tcccgcgtcc 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1440

1381 ctgaaggaaa cagggtttag accgcctcgg ctttagaccc tccgcggcgg cgtgggggag 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1500

1441 atcgcccgcg cccgcttcgc cacgccgcgg ccgtccttcc tttacccgcc cctcccggaa 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1560

1501 gcacgcagcg gcgcggcggc aggggaagag gtagaggtcg gagccccgac ggcgtccccc 1560

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1620

1561 tgccgacgga agccccccct gccccgtccc gccccaagcc gaagaccgca cactggccgc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1680

1621 cgagatctcg gagacgattg gtacaagtac ggaagaagaa aaaggatgtc gaggacccgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(1719) SmaI(1726) .........

1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcccgggt gagccgccac 1740

1681 tgcacgacca ataacacgac agagtagtaa aaccgtttct taagggccca ctcggcggtg 1740

pCAG_F_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 catggctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc 1800

1741 gtaccgacct ctgtactctc gacggttgga aaccggttcg ggcgagtact agtttgcgag 1800

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga 1860

1801 attcttcttg tcggaccgga acagggactg ccggctggtc taccagtcac ggaacaacct 1860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc 1920

1861 acgactcggg gggtatgaga taaggctcat actaggatgg tctgggaagt cacttcgaag 1920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc 1980

1921 ctactacccg aatgactggt tggaccgtct gtccctcgac caagtgtact agttgacccg 1980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg 2040

1981 cttctcccac ggtccgaaac acctaaactg ggaggtacta gtccaggtgg aagatcttac 2040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa 2100

2041 acggaccgat ctctaggact actaaccaga gcagaccgcg aggtacctcg tgggtcactt 2100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat 2160

2101 cgatgacaaa cgaggattga acgagaacct gtccttggtc ccttttacac atctcccgta 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca 2220

2161 ccacctctag aagctgtacg acgaccgatg tagtagagcc aaggcgtact acttagacgt 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt 2280

2221 ccctctcctc aaacacacgg agtttagata ataaaacgaa ttaagacctc acatgtgtaa 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa 2340

2281 agacaggtcg tgggacttca gagaccttct cttcctggta taggtggctc aggacctgtt 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca 2400

2341 ctagtgtctg tgaaactagg tggactaccg gttccgtccg gactgggacg tcgtcgtcgt 2400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg 2460

2401 ggtcgccgac cgggtcgagg aggagtagga gagggtgtag tccgtgtact cattgtttcc 2460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct 2520

2461 gtacctcgta gacatgtcgt acttcacgtt cttgcaccac ggggagatac tggacgacga 2520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga 2580

2521 cctccgccgc ctgcgggtgg cggatgtacg cgggtgatcg gcacctcccc gtaggcacct 2580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa 2640

2581 cctctgcctg gtttcggtga accggtgacg cccgagatga agtagcgtaa ggaacgtttt 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... ......... PvuII(2681) .........

2641 gtattacatc acgggggagg cagagggttt ccctgccaca gctgtcgaca atttactgac 2700

2641 cataatgtag tgccccctcc gtctcccaaa gggacggtgt cgacagctgt taaatgactg 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... AgeI(2725) .......... .......... ..........

2701 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa 2760

2701 gcatgtggtt ttaaacggac gtaatggcca gctacgttgc tcactactcc aagcgttctt 2760

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct 2820

2761 ggactacctg tacaagtccc tagcggtccg caaaagactc gtatggacct tttacgaaga 2820

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc 2880

2821 caggcaaacg gccagcaccc gccgtaccac gttcaactta ttggccttta ccaaagggcg 2880

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... NruI(2898) .......... .......... .......... ..........

2881 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa 2940

2881 tcttggactt ctacaagcgc taatagaaga tatagaagtc cgcgcgccag accgtcattt 2940

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg 3000

2941 ttgataggtc gttgtaaacc cggtcgattt gtacgaagta gcagccaggc ccgacggtgc 3000

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga 3060

3001 tggttcactg tcgttacgac aaagtgacca atacgccgcc taggcttttc ttttgcaact 3060

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... ......... BstBI(3093) ......... ..........

3061 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg 3120

3061 acggccactt gcacgttttg tccgagatcg caagcttgcg tgactaaagc tggtccaagc 3120

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat 3180

3121 aagtgagtac cttttatcgc tagcgacggt cctatatgca ttagaccgta aagaccccta 3180

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... ......... EcoRV(3235)

3181 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc 3240

3181 acgaatattg tgggacaatg catatcggct ttaacggtcc tagtcccaat ttctatagag 3240

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac 3300

3241 tgcatgactg ccaccctctt acaattaggt ataaccgtct tgcttttgcg accaatcgtg 3300

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc 3360

3301 gcgtccacat ctcttccgtg aatcggaccc ccattgattt gaccagctcg ctacctaaag 3360

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg 3420

3361 gcagagacca catcgactac taggcttatt gatggacaaa acggcccagt cttttttacc 3420

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga 3480

3421 acaacggcgc ggtagacggt ggtcggtcga tagttgagcg cgggaccttc cctaaaaact 3480

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

ClaI(3490) .......... .......... .......... .......... ..........

3481 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg 3540

3481 tcgttgagta gctaactaaa tgccgcgatt cctactgaga ccagtctcta tggaccggac 3540

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat 3600

3541 cagacctgtg tcacgggcac agcctcggcg cgctctatac cgggcgcgac ctcaaagtta 3600

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg 3660

3601 tggcctctag tacgttcgac caccgacctg gttacattta taacagtact tgatataggc 3660

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... XhoI(3713)

3661 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc 3720

3661 attggaccta tcactttgtc cccgttacca cgcggacgac cttctaccgc tagagctcgg 3720

.......... CRE_NLS ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc 3780

3721 tagacgacct ctgtactctc gacggttgga aaccggttcg ggcgagtact agtttgcgag 3780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga 3840

3781 attcttcttg tcggaccgga acagggactg ccggctggtc taccagtcac ggaacaacct 3840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc 3900

3841 acgactcggg gggtatgaga taaggctcat actaggatgg tctgggaagt cacttcgaag 3900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc 3960

3901 ctactacccg aatgactggt tggaccgtct gtccctcgac caagtgtact agttgacccg 3960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg 4020

3961 cttctcccac ggtccgaaac acctaaactg ggaggtacta gtccaggtgg aagatcttac 4020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa 4080

4021 acggaccgat ctctaggact actaaccaga gcagaccgcg aggtacctcg tgggtcactt 4080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat 4140

4081 cgatgacaaa cgaggattga acgagaacct gtccttggtc ccttttacac atctcccgta 4140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca 4200

4141 ccacctctag aagctgtacg acgaccgatg tagtagagcc aaggcgtact acttagacgt 4200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt 4260

4201 ccctctcctc aaacacacgg agtttagata ataaaacgaa ttaagacctc acatgtgtaa 4260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa 4320

4261 agacaggtcg tgggacttca gagaccttct cttcctggta taggtggctc aggacctgtt 4320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca 4380

4321 ctagtgtctg tgaaactagg tggactaccg gttccgtccg gactgggacg tcgtcgtcgt 4380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg 4440

4381 ggtcgccgac cgggtcgagg aggagtagga gagggtgtag tccgtgtact cattgtttcc 4440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct 4500

4441 gtacctcgta gacatgtcgt acttcacgtt cttgcaccac ggggagatac tggacgacga 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga 4560

4501 cctccgccgc ctgcgggtgg cggatgtacg cgggtgatcg gcacctcccc gtaggcacct 4560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa 4620

4561 cctctgcctg gtttcggtga accggtgacg cccgagatga agtagcgtaa ggaacgtttt 4620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... NotI(4670) ..........

4621 gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatagc ggccgcactc 4680

4621 cataatgtag tgccccctcc gtctcccaaa gggacggtgt cgaactatcg ccggcgtgag 4680

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca 4740

4681 gagtccacgt ccgacggata gtcttccacc accgaccaca ccggttacgg gaccgagtgt 4740

.......... .......... ....... rb_glob_PA_term ....... ..........

.......... .......... .......... .......... .......... ..........

4741 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg 4800

4741 ttatggtgac tctagaaaaa gggagacggt ttttaatacc cctgtagtac ttcggggaac 4800

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt 4860

4801 tcgtagactg aagaccgatt atttccttta aataaaagta acgttatcac acaaccttaa 4860

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg 4920

4861 aaaacacaga gagtgagcct tcctgtatac cctcccgttt agtaaatttt gtagtcttac 4920

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg 4980

4921 tcataaacca aatctcaaac cgttgtatac ggtatacgac cgacggtact tgtttccacc 4980

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga 5040

4981 gatatttctc cagtagtcat atactttgtc gggggacgac aggtaaggaa taaggtatct 5040

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta 5100

5041 tttcggaact gaactccaat ctaaaaaaaa tataaaacaa aacacaataa aaaaagaaat 5100

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta 5160

5101 tgtagggatt ttaaaaggaa tgtacaaaat gatcggtcta aaaaggagga gaggactgat 5160

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctcccagtca tagctgtccc tcttctctta tgaagatccc tcgacctgca gcccaagctt 5220

5161 gagggtcagt atcgacaggg agaagagaat acttctaggg agctggacgt cgggttcgaa 5220

....... rb_glob_PA_term ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 5280

5221 ccgcattagt accagtatcg acaaaggaca cactttaaca ataggcgagt gttaaggtgt 5280

.......... M13_r .......... M13_pUC_r .......... ..........

.......... .......... .......... .......... .......... ..........

5281 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 5340

5281 gttgtatgct cggccttcgt atttcacatt tcggacccca cggattactc actcgattga 5340

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg 5400

5341 gtgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca gcacggtcgc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 5460

5401 ctaggcgtag agttaatcag tcgttggtat cagggcgggg attgaggcgg gtagggcggg 5460

.......... .......... .......... ......... SV40_origin .........

.......... .......... .......... .......... .......... ..........

5461 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 5520

5461 gattgaggcg ggtcaaggcg ggtaagaggc ggggtaccga ctgattaaaa aaaataaata 5520

.......... ......... SV40_origin SV40_pro ......... ..........

.......... .......... .......... .......... .......... ..........

5521 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 5580

5521 cgtctccggc tccggcggag ccggagactc gataaggtct tcatcactcc tccgaaaaaa 5580

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat 5640

5581 cctccggatc cgaaaacgtt tttcgattga acaaataacg tcgaatatta ccaatgttta 5640

SV40_pro .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

5641 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 5700

5641 tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa aagtgacgta agatcaacac 5700

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg 5760

5701 caaacaggtt tgagtagtta catagaatag tacagaccta ggcgacgtaa ttacttagcc 5760

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 5820

5761 ggttgcgcgc ccctctccgc caaacgcata acccgcgaga aggcgaagga gcgagtgact 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 5880

5821 gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 5940

5881 tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6000

5941 tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg 6000

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

6001 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6060

6001 actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6120

6061 ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 6180

6121 cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagttacgag 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6240

6181 tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6300

6241 tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 6360

6301 ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc 6360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 6420

6361 catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc 6420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6480

6421 ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc 6480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 6540

6481 gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt 6540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 6600

6541 ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact 6600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 6660

6601 gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 6720

6661 gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt catatatact 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 6780

6721 catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac 6780

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

6781 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 6840

6781 agataaagca agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct 6840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 6900

6841 cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg 6900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 6960

6901 tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg 6960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7020

6961 aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg 7020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... FspI(7034) .......... .......... .......... ..........

7021 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 7080

7021 tcaattatca aacgcgttgc aacaacggta acgatgtccg tagcaccaca gtgcgagcag 7080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7140

7081 caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg 7140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7200

7141 gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa 7200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7260

7201 ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg 7260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7320

7261 taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac 7320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 7380

7321 atacgccgct ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc 7380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 7440

7381 gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta 7440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 7500

7441 gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg 7500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 7560

7501 tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt 7560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 7620

7561 tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat 7620

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 7680

7621 aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt 7680

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

7681 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg .......... 7730

7681 tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac .......... 7730

.......... .......... .......... .......... .......... ..........