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    Plasmid map of pCAGIG

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    Gene/insert name: IRES-EGFP
    Alternative names: internal ribosomal entry site from Encephalomyocarditis virus green fluorescent protein from Aequorea victoria
    Insert size (bp): 1318
    Vector backbone: pCAGEN
    Type of vector: Mammalian expression
    Backbone size (bp): 4817
    Cloning site 5': NotI
    Site destroyed during cloning: No
    Cloning site 3': MscI
    Site destroyed during cloning: Yes
    5' Sequencing primer: pCAG-F
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: IRES-GFP was from pMX-IRES-GFP (Nosaka et al., EMBO J. 18, 4754-4765 (1999)) obtained from Dr. T. Kitamura (Univ. of Tokyo).
    Plasmid Provided In: DH5a
    Principal Investigator: Connie Cepko
    Article: Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T et al. (Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. Pubmed)

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