Gene/insert name: IRES-EGFP
Alternative names: internal ribosomal entry site from Encephalomyocarditis virus green fluorescent protein from Aequorea victoria
Insert size (bp): 1318
Vector backbone: pCAGEN
Type of vector: Mammalian expression
Backbone size (bp): 4817
Cloning site 5': NotI
Site destroyed during cloning: No
Cloning site 3': MscI
Site destroyed during cloning: Yes
5' Sequencing primer: pCAG-F
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: IRES-GFP was from pMX-IRES-GFP (Nosaka et al., EMBO J. 18, 4754-4765 (1999)) obtained from Dr. T. Kitamura (Univ. of Tokyo).
Plasmid Provided In: DH5a
Principal Investigator: Connie Cepko
Article: Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T et al. (Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. Pubmed)