Sequence of pCAGIG

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pCAGIG

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pCAGIG : 6135 BP; 1321 A; 1690 C; 1698 G; 1426 T; 0 other;

SalI(1) SpeI(18) .......... .......... .......... ..........

1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60

1 cagctgtaac taataactga tcaataatta tcattagtta atgccccagt aatcaagtat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120

61 cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga ccgactggcg 120

.......... .......... .......... ......... CAG_enhancer .........

.......... .......... .......... .......... .......... ..........

121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180

121 ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat tgcggttatc 180

.......... ......... CAG_enhancer ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240

181 cctgaaaggt aactgcagtt acccacctga taaatgccat ttgacgggtg aaccgtcatg 240

.......... ......... CAG_enhancer ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300

241 tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca tttaccgggc 300

.......... ......... CAG_enhancer ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360

301 ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc atgtagatgc 360

.......... ......... CAG_enhancer ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420

361 ataatcagta gcgataatgg tacccagctc cactcggggt gcaagacgaa gtgagagggg 420

CAG_enhancer ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480

421 tagagggggg ggaggggtgg gggttaaaac ataaataaat aaaaaattaa taaaacacgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 540

481 cgctaccccc gccccccccc cccccgcgcg cggtccgccc cgccccgccc cgctccccgc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600

541 cccgccccgc tccgcctctc cacgccgccg tcggttagtc tcgccgcgcg aggctttcaa 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660

601 aggaaaatac cgctccgccg ccgccgccgc cgggatattt ttcgcttcgc gcgccgcccg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720

661 ccctcagcga cgcaacggaa gcggggcacg gggcgaggcg cggcggagcg cggcgggcgg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780

721 ggccgagact gactggcgca atgagggtgt ccactcgccc gccctgccgg gaagaggagg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840

781 cccgacatta atcgcgaacc aaattactgc cgagcaaaga aaagacaccg acgcactttc 840

.......... .......... .......... ......... Orf frame 1 .........

.......... .......... .......... .......... .......... ..........

841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900

841 ggaatttccc gaggccctcc cgggaaacac gccccccctc gccgagcccc ccacgcacgc 900

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960

901 acacacacac gcacccctcg cggcgcacgc cgggcgcgac gggccgccga cactcgcgac 960

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020

961 gcccgcgccg cgccccgaaa cacgcgaggc gcacacgcgc tcccctcgcg ccggcccccg 1020

.......... ......... Orf frame 1 ......... .......... ..........

SacII(1030) ........ .......... .......... .......... ..........

1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080

1021 ccacggggcg ccacgccccc ccgacgctcc ccttgtttcc gacgcacgcc ccacacacgc 1080

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140

1081 acccccccac tcgtccccca cacccgcgcc gccagcccga cattgggggg ggacgtgggg 1140

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200

1141 ggaggggctc aacgactcgt gccgggccga agcccacgcc ccgaggcacg ccccgcaccg 1200

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260

1201 cgccccgagc ggcacggccc gccccccacc gccgtccacc cccacggccc gccccgcccc 1260

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320

1261 ggcggagccc ggcccctccc gagccccctc cccgcgccgc cggggcctcg cggccgccga 1320

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380

1321 cagctccgcg ccgctcggcg tcggtaacgg aaaataccat tagcacgctc tcccgcgtcc 1380

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1440

1381 ctgaaggaaa cagggtttag accgcctcgg ctttagaccc tccgcggcgg cgtgggggag 1440

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1500

1441 atcgcccgcg cccgcttcgc cacgccgcgg ccgtccttcc tttacccgcc cctcccggaa 1500

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1560

1501 gcacgcagcg gcgcggcggc aggggaagag gtagaggtcg gagccccgac ggcgtccccc 1560

......... Orf frame 1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1620

1561 tgccgacgga agccccccct gccccgtccc gccccaagcc gaagaccgca cactggccgc 1620

.......... .......... .......... .......... .......... ..........

XbaI(1623) .......... .......... .......... .......... ..........

1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1680

1621 cgagatctcg gagacgattg gtacaagtac ggaagaagaa aaaggatgtc gaggacccgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... ... EcorI(1719) XhoI(1725) EcoRV(1733),NotI(1738)

1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcctcgag gatatcgcgg 1740

1681 tgcacgacca acaacacgac agagtagtaa aaccgtttct taaggagctc ctatagcgcc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccgctacgta aattccgccc cccccccccc tctccctccc ccccccctaa cgttactggc 1800

1741 ggcgatgcat ttaaggcggg gggggggggg agagggaggg gggggggatt gcaatgaccg 1800

.......... .......... .......... .......... IRES ..........

.......... .......... .......... .......... .......... ..........

1801 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 1860

1801 gcttcggcga accttattcc ggccacacgc aaacagatat acaataaaag gtggtataac 1860

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 1920

1861 ggcagaaaac cgttacactc ccgggccttt ggaccgggac agaagaactg ctcgtaagga 1920

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 1980

1921 tccccagaaa ggggagagcg gtttccttac gttccagaca acttacagca cttccttcgt 1980

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 2040

1981 caaggagacc ttcgaagaac ttctgtttgt tgcagacatc gctgggaaac gtccgtcgcc 2040

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... PmlI(2083) ..........

2041 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 2100

2041 ttggggggtg gaccgctgtc cacggagacg ccggttttcg gtgcacatat tctatgtgga 2100

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 2160

2101 cgtttccgcc gtgttggggt cacggtgcaa cactcaacct atcaacacct ttctcagttt 2160

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... KpnI(2212)

2161 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 2220

2161 accgagagga gttcgcataa gttgttcccc gacttcctac gggtcttcca tggggtaaca 2220

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 2280

2221 taccctagac tagaccccgg agccacgtgt acgaaatgta cacaaatcag ctccaatttt 2280

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 2340

2281 tttgcagatc cggggggctt ggtgcccctg caccaaaagg aaactttttg tgctactatt 2340

.......... .......... IRES .......... .......... ..........

MscI(2345) .......... .......... .......... .......... ..........

2341 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 2400

2341 ataccggtgt tggtaccact cgttcccgct cctcgacaag tggccccacc acgggtagga 2400

IRES .......... ....... Orf frame 2 EGFP ....... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 2460

2401 ccagctcgac ctgccgctgc atttgccggt gttcaagtcg cacaggccgc tcccgctccc 2460

EGFP_N_primer ... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 2520

2461 gctacggtgg atgccgttcg actgggactt caagtagacg tggtggccgt tcgacgggca 2520

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 2580

2521 cgggaccggg tgggagcact ggtgggactg gatgccgcac gtcacgaagt cggcgatggg 2580

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 2640

2581 gctggtgtac ttcgtcgtgc tgaagaagtt caggcggtac gggcttccga tgcaggtcct 2640

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 2700

2641 cgcgtggtag aagaagttcc tgctgccgtt gatgttctgg gcgcggctcc acttcaagct 2700

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 2760

2701 cccgctgtgg gaccacttgg cgtagctcga cttcccgtag ctgaagttcc tcctgccgtt 2760

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 2820

2761 gtaggacccc gtgttcgacc tcatgttgat gttgtcggtg ttgcagatat agtaccggct 2820

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 2880

2821 gttcgtcttc ttgccgtagt tccacttgaa gttctaggcg gtgttgtagc tcctgccgtc 2880

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 2940

2881 gcacgtcgag cggctggtga tggtcgtctt gtgggggtag ccgctgccgg ggcacgacga 2940

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 3000

2941 cgggctgttg gtgatggact cgtgggtcag gcgggactcg tttctggggt tgctcttcgc 3000

.......... ....... Orf frame 2 EGFP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 3060

3001 gctagtgtac caggacgacc tcaagcactg gcggcggccc tagtgagagc cgtacctgct 3060

..... EGFP_C_primer Orf frame 2 EGFP ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gctgtacaag taaagcggcc ggccgccagc acagtggtcg atcgacctgc aggcatgcaa 3120

3061 cgacatgttc atttcgccgg ccggcggtcg tgtcaccagc tagctggacg tccgtacgtt 3120

Orf frame 2 EGFP .... .......... .......... .......... ..........

.......... .......... ......... BglII(3156) ......... ..........

3121 gcttcagcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat 3180

3121 cgaagtcggt tacgggaccg agtgtttatg gtgactctag aaaaagggag acggttttta 3180

.......... .......... .......... .... rb_glob_PA_terminator ....

.......... .......... .......... .......... .......... ..........

3181 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 3240

3181 atacccctgt agtacttcgg ggaactcgta gactgaagac cgattatttc ctttaaataa 3240

.......... .... rb_glob_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 3300

3241 aagtaacgtt atcacacaac cttaaaaaac acagagagtg agccttcctg tataccctcc 3300

.......... .... rb_glob_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata 3360

3301 cgtttagtaa attttgtagt cttactcata aaccaaatct caaaccgttg tatacggtat 3360

.......... .... rb_glob_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc 3420

3361 acgaccgacg gtacttgttt ccaccgatat ttctccagta gtcatatact ttgtcggggg 3420

.......... .... rb_glob_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt 3480

3421 acgacaggta aggaataagg tatcttttcg gaactgaact ccaatctaaa aaaaatataa 3480

.......... .... rb_glob_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc 3540

3481 aacaaaacac aataaaaaaa gaaattgtag ggattttaaa aggaatgtac aaaatgatcg 3540

.......... .... rb_glob_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag 3600

3541 gtctaaaaag gaggagagga ctgatgaggg tcagtatcga cagggagaag agaatacttc 3600

.......... .... rb_glob_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 3660

3601 tagggagctg gacgtcgggt tcgaaccgca ttagtaccag tatcgacaaa ggacacactt 3660

.......... .......... .......... .......... M13_r M13_pUC_r

.......... .......... .......... .......... .......... ..........

3661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 3720

3661 taacaatagg cgagtgttaa ggtgtgttgt atgctcggcc ttcgtatttc acatttcgga 3720

M13_pUC_r .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

3721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 3780

3721 ccccacggat tactcactcg attgagtgta attaacgcaa cgcgagtgac gggcgaaagg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc 3840

3781 tcagcccttt ggacagcacg gtcgcctagg cgtagagtta atcagtcgtt ggtatcaggg 3840

.......... .......... .......... .......... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

3841 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 3900

3841 cggggattga ggcgggtagg gcggggattg aggcgggtca aggcgggtaa gaggcggggt 3900

.......... .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 3960

3901 accgactgat taaaaaaaat aaatacgtct ccggctccgg cggagccgga gactcgataa 3960

... SV40_origin SV40_pro SV40pro_F_pri ... .......... ..........

.......... .......... StuI(3990) .......... .......... ..........

3961 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt 4020

3961 ggtcttcatc actcctccga aaaaacctcc ggatccgaaa acgtttttcg attgaacaaa 4020

.......... .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 4080

4021 taacgtcgaa tattaccaat gtttatttcg ttatcgtagt gtttaaagtg tttatttcgt 4080

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 4140

4081 aaaaaaagtg acgtaagatc aacaccaaac aggtttgagt agttacatag aatagtacag 4140

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 4200

4141 acctaggcga cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 4260

4201 cgagaaggcg aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 4320

4261 atagtcgagt gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 4380

4321 tcttgtacac tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc 4380

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 4440

4381 gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 4500

4441 ccaccgcttt gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4560

4501 acgcgagagg acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc 4620

4561 cttcgcaccg cgaaagagtt acgagtgcga catccataga gtcaagccac atccagcaag 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4680

4621 cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4740

4681 cattgatagc agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4800

4741 gaccattgtc ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 4860

4801 ccggattgat gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4920

4861 aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4980

4921 caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 5040

4981 gaaactagaa aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa 5040

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 5100

5041 accagtactc taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 5160

5101 aatttagtta gatttcatat atactcattt gaaccagact gtcaatggtt acgaattagt 5160

.......... .......... .......... .......... ....... ORF2 Ampicillin

.......... .......... .......... .......... .......... ..........

5161 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 5220

5161 cactccgtgg atagagtcgc tagacagata aagcaagtag gtatcaacgg actgaggggc 5220

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 5280

5221 agcacatcta ttgatgctat gccctcccga atggtagacc ggggtcacga cgttactatg 5280

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 5340

5281 gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt cggccttccc 5340

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 5400

5341 ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta ggtcagataa ttaacaacgg 5400

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... FspI(5439) .......... ..........

5401 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 5460

5401 cccttcgatc tcattcatca agcggtcaat tatcaaacgc gttgcaacaa cggtaacgat 5460

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 5520

5461 gtccgtagca ccacagtgcg agcagcaaac cataccgaag taagtcgagg ccaagggttg 5520

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 5580

5521 ctagttccgc tcaatgtact agggggtaca acacgttttt tcgccaatcg aggaagccag 5580

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 5640

5581 gaggctagca acagtcttca ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg 5640

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 5700

5641 acgtattaag agaatgacag tacggtaggc attctacgaa aagacactga ccactcatga 5700

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 5760

5701 gttggttcag taagactctt atcacatacg ccgctggctc aacgagaacg ggccgcagtt 5760

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 5820

5761 atgccctatt atggcgcggt gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa 5820

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 5880

5821 gaagccccgc ttttgagagt tcctagaatg gcgacaactc taggtcaagc tacattgggt 5880

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 5940

5881 gagcacgtgg gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt 5940

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 6000

5941 tttgtccttc cgttttacgg cgttttttcc cttattcccg ctgtgccttt acaacttatg 6000

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 6060

6001 agtatgagaa ggaaaaagtt ataataactt cgtaaatagt cccaataaca gagtactcgc 6060

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

6061 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 6120

6061 ctatgtataa acttacataa atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg 6120

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gaaaagtgcc acctg..... .......... .......... .......... .......... 6135

6121 cttttcacgg tggac..... .......... .......... .......... .......... 6135

.......... .......... .......... .......... .......... ..........