k7k7HID FJ362600; SV 1; circular; other DNA; STD; SYN; 11375 BP. AC FJ362600; DT 16-DEC-2008 (Rel. 98, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 1) DE Binary vector pCAMBIA1300-TMT-2S, complete sequence. KW . OS Binary vector pCAMBIA1300-TMT-2S OC other sequences; artificial sequences; vectors. RN [1] RP 1-11375 RA Shi X.G., Zhuang W.J.; RT "Binary vector pCAMBIA1300-TMT-2S for making gamma-tocopherol RT methyltransferase over expression in seed"; RL Unpublished. RN [2] RP 1-11375 RA Zhuang W.J., Shi X.G.; RT ; RL Submitted (08-OCT-2008) to the EMBL/GenBank/DDBJ databases. RL Crop Science Department, Oil Crop Institution, Fujian Agriculture, Jinshan, RL Fuzhou, Fujian 350002, P.R. China FH Key Location/Qualifiers FT source 1..11375 FT /organism="Binary vector pCAMBIA1300-TMT-2S" FT /mol_type="other DNA" FT /db_xref="taxon:571925" FT terminator 8..260 FT /note="NOS" FT gene complement(277..1323) FT /gene="VTE4" FT CDS complement(277..1323) FT /codon_start=1 FT /transl_table=11 FT /gene="VTE4" FT /product="gamma-tocopherol methyltransferase" FT /note="derived from Arabidopsis thaliana" FT /protein_id="ACJ54270.1" FT /translation="MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRSPSSSSSVSMTTTR FT GNVAVAAAATSTEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEA FT QIRMIEESLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAK FT RANDLAAAQSLSHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAA FT PGGRIIIVTWCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQD FT IKCADWSENVAPFWPAVIRTALTWKGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFG FT IITCQKPL" FT promoter 1341..2501 FT /note="Brassica napus 2S seed storage protein promoter" FT misc_feature 2746..2771 FT /note="right border T-DNA repeat" FT CDS complement(7786..8580) FT /codon_start=1 FT /transl_table=11 FT /product="aminoglycoside phosphotransferase" FT /protein_id="ACJ54268.1" FT /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM FT TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED FT EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT FT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FT FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" FT misc_feature 9005..9030 FT /note="left border C58 T-DNA repeat" FT misc_feature 9097..9305 FT /note="CaMV polyA signal" FT CDS complement(9321..10346) FT /codon_start=1 FT /transl_table=11 FT /product="hygromycin phosphotransferase" FT /note="hptII; confers hygromycin resistance" FT /protein_id="ACJ54269.1" FT /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG FT YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP FT ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ FT TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF FT GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG FT NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK FT K" FT promoter 10382..11162 FT /note="CaMV 35S promoter" SQ Sequence 11375 BP; 2836 A; 2946 C; 2929 G; 2664 T; 0 other; aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240 tgccaaatgt ttgaacgatc ggggaaattc gagctcttag agtggcttct ggcaagtgat 300 gataccaaac ttaatgacac ctttcttgta accttcaatc atcaatggca ttgtcaatgc 360 tcctttaata cttttcatac cactacgaag cagagacaca aggcccttcc atgttaatgc 420 agtccgtata accgcaggcc agaaaggagc tacgttctct gaccaatccg cacacttaat 480 atcctggaga gaatgggatt gaagcaagtt gacataatca tcggtggagc accaagccgg 540 gagatagaac gtcttacaga ttttgtccaa gatgttttgc tcccacggct gcaaagcttc 600 ctcccccgca gatagatttc tatggcacca tgtcactatt attatcctac ctcctggagc 660 cgccacacgt accaactctt ttacaaactt ggccttgtca ggcatatgct caccactctc 720 catcgaccac actagatcga attttccatc ttcgaatggc tgatccaacg catccgcaac 780 ttggaaggaa gccttatgag agagtgattg agcagccgcg agatcattgg ctctcttggc 840 ctgaacaggg ctgagagtaa tgccaatgca ttcagctcca aatttagagg caagatatct 900 tgagcttcct ccaatcccac acccaacatc cactactttc tttatctttt tctcctcctc 960 ttcatcagta acaccggcga aacggagaga ctcttcaatc atacggatct gagcttcctt 1020 gtgaccagaa tcagaaagtt gaacagaaga atcagggtca taaaagccat gatgcatatg 1080 atctccccaa atctcttccc acaaacccga agtttcattg tagaactccg ctattccttt 1140 tcttagcgcc tcagtggatg tagcagcagc cgccacagcc acgtttccac gcgttgtcgt 1200 catagagact gaggaggagg aggatggaga ccgaaagaga agcgatgact ttgagccgaa 1260 agaagagttg gttcgataag ggaggcttgt gagagaagag ggtgctgcta gagttgcttt 1320 catggtgact ctagacccgg gtcgtgtatg tttttaatct tgtttgtatt gatgagtttt 1380 ggtttgagta aagagtgaag cggatgagtt aatttatagg ctataaagga gatttgcatg 1440 gcgatcacgt gtaataatgc atgcacgcat gtgattgtat gtgtgtgctg tgagagagaa 1500 gctcttaggt gtttgaaggg agtgacaagt ggcgaagaaa aacaattctc cgcggctgca 1560 tgctatgtgt aacgtgtagc taatgttctg gcatggcatc ttatgaacga ttctttttaa 1620 aaacaaggta aaaacttaac ttcataaaat taaaaaaaaa aaacgtttac taagttggtt 1680 taaaagggga tgagagtcta taaattttgg aggtagtgcc gttgggaata taaattggga 1740 gcttaatcag aattatagaa gttaaagttg atttagtcac ggtcaatata aattgggaat 1800 ttgagtcaaa atcttccaaa ttcggaatcc gtcttgttac acccggtgga taggagccga 1860 acggtttgaa aatacttgaa atgtggatgc aggtgcaggc tggtttaatt ttatgttgaa 1920 tggatacatg tcaatcgaat ttgagttata ggtacacatt ttactctgat actaaaatgt 1980 aacatttgtc tcaagaatgg gtaggtcatc cttactcgag aacggtatgc cattcacttg 2040 aacgtatatc tcattcaacc caactctaac acacatttca tacccatgtc taaaataaag 2100 aaaattgaag aaaaacgctc aatagcgtca atccttacaa atataatgta catggcaacc 2160 tcgcatgaaa atagtaataa agaggagtga ataatataaa gtagtcacta attggatgac 2220 aaagcaaatg gaaccatccg atagatgttt tgagaacaag aggtacattt gtatatatgc 2280 attggttgat acatttctgc catctgaatt gtttcttcct caaagaagaa gcattcagcc 2340 aatagtgaaa tttaaaatgt tggaaaaatg aggatgcagt cagaaagtcc agaatataag 2400 tggtaactgg taactgaata cttgcctcag aagcaagata agaaacataa gtctttaaag 2460 gaatcaatca ccgatgaaga aagcttctgc aggcatgcaa gcttggcact ggccgtcgtt 2520 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 2580 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 2640 ttgcgcagcc tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc 2700 cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa 2760 aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc 2820 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac 2880 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg 2940 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt 3000 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt 3060 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg 3120 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt 3180 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc 3240 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg 3300 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag 3360 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca 3420 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca 3480 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg 3540 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg 3600 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg 3660 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg 3720 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc 3780 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc 3840 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa 3900 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag 3960 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg 4020 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa 4080 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg 4140 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg 4200 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg 4260 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg 4320 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac 4380 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat 4440 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt 4500 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag 4560 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag 4620 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga 4680 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga 4740 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc 4800 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc 4860 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga 4920 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa 4980 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca 5040 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag 5100 gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 5160 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga 5220 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 5280 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 5340 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 5400 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 5460 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta 5520 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 5580 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 5640 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 5700 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 5760 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 5820 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 5880 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 5940 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 6000 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 6060 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 6120 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 6180 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 6240 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 6300 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 6360 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 6420 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 6480 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 6540 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 6600 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg 6660 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 6720 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 6780 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 6840 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 6900 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 6960 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7020 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7080 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7140 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7200 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7260 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7320 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7380 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7440 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7500 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7560 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7620 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7680 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7740 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc 7800 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa 7860 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat 7920 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc 7980 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc 8040 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc 8100 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc 8160 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag 8220 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc 8280 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg 8340 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt 8400 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag 8460 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt 8520 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat 8580 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac 8640 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct 8700 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa 8760 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc 8820 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta 8880 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg 8940 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg 9000 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat 9060 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag 9120 tactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa 9180 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa 9240 ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac 9300 agatccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg gcgtcggttt 9360 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg 9420 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc 9480 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca 9540 atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg cctccgctcg 9600 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg 9660 acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg 9720 ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg 9780 gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg 9840 gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa 9900 tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc 9960 tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg tagaacagcg 10020 ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa 10080 taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc 10140 gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc 10200 cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc 10260 gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac 10320 gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc gaaagctcga 10380 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga 10440 acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg 10500 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt 10560 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct 10620 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct 10680 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat 10740 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata 10800 ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt 10860 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct 10920 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg 10980 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg 11040 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt 11100 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc 11160 atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 11220 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 11280 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 11340 gtatgttgtg tggaattgtg agcggataac aattt 11375 o,pCAMBIA1300-TMT-2S"Times New Roman k7+SacI(276),HindIII(595),NdeI(705),EcoRV(897) ArialSo,vlb"b k76NdeI(1077),MluI(1190),XbaI(1331),XmaI(1337),SmaI(1339) Arial3o,T[aaZ k7HXhoI(2016),HindIII(2482),PstI(2492),HindIII(2500),PvuII(2595),PmeI(2713) Arial >o,!@ k76ScaI(2868),EagI(3158),EagI(3386),EagI(3390),SfiI(3481) Arial: o,{ k7cEagI(3716),NotI(3716),EagI(3820),SfiI(3915),SfiI(3924),EagI(4194),EagI(4270),NdeI(4385),SexAI(4401) ArialTo,yH k77EagI(4731),SexAI(5164),SfiI(5190),NotI(5248),EagI(5248) ArialFo,.;JJZ k7oEagI(5494),SfiI(5502),SexAI(5524),EagI(5597),SexAI(5674),EagI(5744),EagI(5817),NheI(5840),ClaI(5920),EagI(5933) Arial*o,6@XX k7 EagI(6538),NotI(6538),NdeI(6874) Arialo,VQ(L00LT k7EcoRV Arialo, k76BclI(8768),ScaI(9122),XhoI(9283),ScaI(9376),EagI(9402) ArialC$o,  k7RNdeI(9894),EagI(9972),EagI(10137),XmaI(10360),SmaI(10362),XhoI(10377),EcoRV(10512) Arialx(o,=82~*2~ k7PvuII Arial+o,kaWKWk7o,+VTE4"Times New Romank7CɆo, M13_forward20_primer"Times New Romank7o, M13_pUC_fwd_primer"Times New Romank7Co, ORF 3"Times New Romank7Co,HORF 1"Times New Romank7o,[ORF 2"Times New Romank7o,%ORF 2"Times New Romank7Co,+ pGEX_3_primer"Times New Romank7Co,j!KanR1"Times New Romank7Co,j!ORF 3"Times New Romank7Co,9$'ORF 4"Times New Romank7o,i$j(ORF 1"Times New Romank7Co,6,S, lac_promoter"Times New Roman k7test"Times New Roman *Vo,U%)p!p/