Sequence of pCAMBIA1300-TMT-2S

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pCAMBIA1300-TMT-2S

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pCAMBIA1300-TMT-2S: 11375 BP; 2836 A; 2946 C; 2929 G; 2664 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60

1 ttaagggcta gatcattgta tctactgtgg cgcgcgctat taaataggat caaacgcgcg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120

61 atataaaaca aaagatagcg cataatttac atattaacgc cctgagatta gtatttttgg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180

121 gtagagtatt tattgcagta cgtaatgtac aattaataat gtacgaattg cattaagttg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240

181 tctttaatat actattagta gcgttctggc cgttgtccta agttagaatt ctttgaaata 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgccaaatgt ttgaacgatc ggggaaattc gagctcttag agtggcttct ggcaagtgat 300

241 acggtttaca aacttgctag cccctttaag ctcgagaatc tcaccgaaga ccgttcacta 300

.......... .......... .......... .......... VTE4 ..........

.......... .......... .......... .......... .......... ..........

301 gataccaaac ttaatgacac ctttcttgta accttcaatc atcaatggca ttgtcaatgc 360

301 ctatggtttg aattactgtg gaaagaacat tggaagttag tagttaccgt aacagttacg 360

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcctttaata cttttcatac cactacgaag cagagacaca aggcccttcc atgttaatgc 420

361 aggaaattat gaaaagtatg gtgatgcttc gtctctgtgt tccgggaagg tacaattacg 420

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agtccgtata accgcaggcc agaaaggagc tacgttctct gaccaatccg cacacttaat 480

421 tcaggcatat tggcgtccgg tctttcctcg atgcaagaga ctggttaggc gtgtgaatta 480

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcctggaga gaatgggatt gaagcaagtt gacataatca tcggtggagc accaagccgg 540

481 taggacctct cttaccctaa cttcgttcaa ctgtattagt agccacctcg tggttcggcc 540

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gagatagaac gtcttacaga ttttgtccaa gatgttttgc tcccacggct gcaaagcttc 600

541 ctctatcttg cagaatgtct aaaacaggtt ctacaaaacg agggtgccga cgtttcgaag 600

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ctcccccgca gatagatttc tatggcacca tgtcactatt attatcctac ctcctggagc 660

601 gagggggcgt ctatctaaag ataccgtggt acagtgataa taataggatg gaggacctcg 660

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgccacacgt accaactctt ttacaaactt ggccttgtca ggcatatgct caccactctc 720

661 gcggtgtgca tggttgagaa aatgtttgaa ccggaacagt ccgtatacga gtggtgagag 720

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 catcgaccac actagatcga attttccatc ttcgaatggc tgatccaacg catccgcaac 780

721 gtagctggtg tgatctagct taaaaggtag aagcttaccg actaggttgc gtaggcgttg 780

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttggaaggaa gccttatgag agagtgattg agcagccgcg agatcattgg ctctcttggc 840

781 aaccttcctt cggaatactc tctcactaac tcgtcggcgc tctagtaacc gagagaaccg 840

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... EcoRV(897)

841 ctgaacaggg ctgagagtaa tgccaatgca ttcagctcca aatttagagg caagatatct 900

841 gacttgtccc gactctcatt acggttacgt aagtcgaggt ttaaatctcc gttctataga 900

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgagcttcct ccaatcccac acccaacatc cactactttc tttatctttt tctcctcctc 960

901 actcgaagga ggttagggtg tgggttgtag gtgatgaaag aaatagaaaa agaggaggag 960

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttcatcagta acaccggcga aacggagaga ctcttcaatc atacggatct gagcttcctt 1020

961 aagtagtcat tgtggccgct ttgcctctct gagaagttag tatgcctaga ctcgaaggaa 1020

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtgaccagaa tcagaaagtt gaacagaaga atcagggtca taaaagccat gatgcatatg 1080

1021 cactggtctt agtctttcaa cttgtcttct tagtcccagt attttcggta ctacgtatac 1080

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atctccccaa atctcttccc acaaacccga agtttcattg tagaactccg ctattccttt 1140

1081 tagaggggtt tagagaaggg tgtttgggct tcaaagtaac atcttgaggc gataaggaaa 1140

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcttagcgcc tcagtggatg tagcagcagc cgccacagcc acgtttccac gcgttgtcgt 1200

1141 agaatcgcgg agtcacctac atcgtcgtcg gcggtgtcgg tgcaaaggtg cgcaacagca 1200

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 catagagact gaggaggagg aggatggaga ccgaaagaga agcgatgact ttgagccgaa 1260

1201 gtatctctga ctcctcctcc tcctacctct ggctttctct tcgctactga aactcggctt 1260

.......... .......... VTE4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agaagagttg gttcgataag ggaggcttgt gagagaagag ggtgctgcta gagttgcttt 1320

1261 tcttctcaac caagctattc cctccgaaca ctctcttctc ccacgacgat ctcaacgaaa 1320

.......... .......... VTE4 .......... .......... ..........

XbaI(1331) XmaI(1337),SmaI(1339) ........ .......... ..........

1321 catggtgact ctagacccgg gtcgtgtatg tttttaatct tgtttgtatt gatgagtttt 1380

1321 gtaccactga gatctgggcc cagcacatac aaaaattaga acaaacataa ctactcaaaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggtttgagta aagagtgaag cggatgagtt aatttatagg ctataaagga gatttgcatg 1440

1381 ccaaactcat ttctcacttc gcctactcaa ttaaatatcc gatatttcct ctaaacgtac 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcgatcacgt gtaataatgc atgcacgcat gtgattgtat gtgtgtgctg tgagagagaa 1500

1441 cgctagtgca cattattacg tacgtgcgta cactaacata cacacacgac actctctctt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctcttaggt gtttgaaggg agtgacaagt ggcgaagaaa aacaattctc cgcggctgca 1560

1501 cgagaatcca caaacttccc tcactgttca ccgcttcttt ttgttaagag gcgccgacgt 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgctatgtgt aacgtgtagc taatgttctg gcatggcatc ttatgaacga ttctttttaa 1620

1561 acgatacaca ttgcacatcg attacaagac cgtaccgtag aatacttgct aagaaaaatt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaacaaggta aaaacttaac ttcataaaat taaaaaaaaa aaacgtttac taagttggtt 1680

1621 tttgttccat ttttgaattg aagtatttta attttttttt tttgcaaatg attcaaccaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 taaaagggga tgagagtcta taaattttgg aggtagtgcc gttgggaata taaattggga 1740

1681 attttcccct actctcagat atttaaaacc tccatcacgg caacccttat atttaaccct 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcttaatcag aattatagaa gttaaagttg atttagtcac ggtcaatata aattgggaat 1800

1741 cgaattagtc ttaatatctt caatttcaac taaatcagtg ccagttatat ttaaccctta 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttgagtcaaa atcttccaaa ttcggaatcc gtcttgttac acccggtgga taggagccga 1860

1801 aactcagttt tagaaggttt aagccttagg cagaacaatg tgggccacct atcctcggct 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acggtttgaa aatacttgaa atgtggatgc aggtgcaggc tggtttaatt ttatgttgaa 1920

1861 tgccaaactt ttatgaactt tacacctacg tccacgtccg accaaattaa aatacaactt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tggatacatg tcaatcgaat ttgagttata ggtacacatt ttactctgat actaaaatgt 1980

1921 acctatgtac agttagctta aactcaatat ccatgtgtaa aatgagacta tgattttaca 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aacatttgtc tcaagaatgg gtaggtcatc cttactcgag aacggtatgc cattcacttg 2040

1981 ttgtaaacag agttcttacc catccagtag gaatgagctc ttgccatacg gtaagtgaac 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aacgtatatc tcattcaacc caactctaac acacatttca tacccatgtc taaaataaag 2100

2041 ttgcatatag agtaagttgg gttgagattg tgtgtaaagt atgggtacag attttatttc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaaattgaag aaaaacgctc aatagcgtca atccttacaa atataatgta catggcaacc 2160

2101 ttttaacttc tttttgcgag ttatcgcagt taggaatgtt tatattacat gtaccgttgg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcgcatgaaa atagtaataa agaggagtga ataatataaa gtagtcacta attggatgac 2220

2161 agcgtacttt tatcattatt tctcctcact tattatattt catcagtgat taacctactg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aaagcaaatg gaaccatccg atagatgttt tgagaacaag aggtacattt gtatatatgc 2280

2221 tttcgtttac cttggtaggc tatctacaaa actcttgttc tccatgtaaa catatatacg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 attggttgat acatttctgc catctgaatt gtttcttcct caaagaagaa gcattcagcc 2340

2281 taaccaacta tgtaaagacg gtagacttaa caaagaagga gtttcttctt cgtaagtcgg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aatagtgaaa tttaaaatgt tggaaaaatg aggatgcagt cagaaagtcc agaatataag 2400

2341 ttatcacttt aaattttaca acctttttac tcctacgtca gtctttcagg tcttatattc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tggtaactgg taactgaata cttgcctcag aagcaagata agaaacataa gtctttaaag 2460

2401 accattgacc attgacttat gaacggagtc ttcgttctat tctttgtatt cagaaatttc 2460

.......... .......... .......... .......... .......... ..........

.......... ........ HindIII(2482) PstI(2492) ........ ..........

2461 gaatcaatca ccgatgaaga aagcttctgc aggcatgcaa gcttggcact ggccgtcgtt 2520

2461 cttagttagt ggctacttct ttcgaagacg tccgtacgtt cgaaccgtga ccggcagcaa 2520

.......... .......... .......... .......... ..... M13_forward20_primer

.......... .......... .......... .......... .......... ..........

2521 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 2580

2521 aatgttgcag cactgaccct tttgggaccg caatgggttg aattagcgga acgtcgtgta 2580

M13_pUC_fwd_primer .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 2640

2581 gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt ggctagcggg aagggttgtc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ttgcgcagcc tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc 2700

2641 aacgcgtcgg acttaccgct tacgatctcg tcgaactcga acctagtcta acagcaaagg 2700

.......... .......... .......... .......... .......... ..........

.......... PmeI(2713) .......... .......... .......... ..........

2701 cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa 2760

2701 gcggaagtca aatttgatag tcacaaactg tcctatataa ccgcccattt ggattctctt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc 2820

2761 ttctcgcaaa taatcttatt gcctataaat tttcccgcac ttttccaaat aggcaagcag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac 2880

2821 gtaaacatac acgtacggtt ggtgtcccaa ggggagccct agtttcatga aactaggttg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg 2940

2881 gggaggcgac gatatcacgt cagccgaaga ctgcaagtca cgtcggcaga agacttttgc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt 3000

2941 tgtacagcgt gttcaggatt caatgcgctg tccgacggcg ggacgggaaa aggaccgcaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt 3060

3001 aagaacagcg cacaaaatca gcgtatttca tcttatgaac gctgatcttg gcctctgtaa 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg 3120

3061 tgcggtactt gttctcgcgg cggcgaccgg acgacccgat acgggcgcag tcgtggctgc 3120

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

3121 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt 3180

3121 tggtcctgaa ctggttggtt gcccggcttg acgtgcgccg gccgacgtgg ttcgacaaaa 3180

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc 3240

3181 ggctcttcta gtggccgtgg tccgcgctgg cgggcctcga ccggtcctac gaactggtgg 3240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg 3300

3241 atgcgggacc gctgcaacac tgtcactggt ccgatctggc ggaccgggcg tcgtgggcgc 3300

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag 3360

3301 tggatgacct gtaacggctc gcgtaggtcc tccggccgcg cccggacgca tcggaccgtc 3360

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca 3420

3361 tcggcacccg gctgtggtgg tgcggccggc cggcgtacca caactggcac aagcggccgt 3420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca 3480

3421 aacggctcaa gctcgcaagg gattagtagc tggcgtgggc ctcgcccgcg ctccggcggt 3480

.......... .......... ORF 3 .......... .......... ..........

SfiI(3481) .......... .......... .......... .......... ..........

3481 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg 3540

3481 tccgggctcc gcacttcaaa ccgggggcgg gatgggagtg gggccgtgtc tagcgcgtgc 3540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg 3600

3541 gggcgctcga ctagctggtc cttccggcgt ggcactttct ccgccgacgt gacgaaccgc 3600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg 3660

3601 acgtagcgag ctgggacatg gcgcgtgaac tcgcgtcgct ccttcactgc gggtggctcc 3660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg 3720

3661 ggtccgccgc gccacggaag gcactcctgc gtaactggct ccggctgcgg gaccgccggc 3720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc 3780

3721 ggctcttact tgcggttctc cttgttcgta ctttggcgtg gtcctgccgg tcctgcttgg 3780

.......... ORF 3 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc 3840

3781 caaaaagtaa tggcttctct agctccgcct ctactagcgc cggcccatgc acaagctcgg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa 3900

3841 cgggcgcgtg cagagttggc acgccgacgt actttaggac cggccaaaca gactacggtt 3900

.......... .......... .......... .......... .......... ..........

.......... SfiI(3915) .......... .......... .......... ..........

3901 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag 3960

3901 cgaccgccgg accggccggt cgaaccggcg acttctttgg ctcgcggcgg cagatttttc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg 4020

3961 cactacacat aaactcattt tgtcgaacgc agtacgccag cgacgcatat actacgctac 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa 4080

4021 tcatttattt gtttatgcgt tccccttgcg tacttccaat agcgacatga attggtcttt 4080

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

4081 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg 4140

4081 ccgcccagtc cgttctgctg gtagcgttgg gtagatcggg cgcgggacgt tgagcggccc 4140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg 4200

4141 cggctacaag acaatcagct aaggctaggg gtcccgtcac gggcgctaac ccgccggcac 4200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg 4260

4201 gcccttctag ttggcgattg gcaacagccg tagctggcgg gctgctaact ggcgctgcac 4260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg 4320

4261 ttccggtagc cggccgcgct gaagcatcac tagctgcctc gcggggtccg ccgcctgaac 4320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac 4380

4321 cgacacaggc gctagttccg tcggctgaag cacgactaag gccacgtcgg ttcgggaatg 4380

.......... .......... ORF 1 .......... .......... ..........

NdeI(4385) ......... SexAI(4401) ......... .......... ..........

4381 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat 4440

4381 ctgtataccc ggtggcggct ggaccacctc gaccaattcg tcgcgtaact ccagtgccta 4440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt 4500

4441 ccttccgatg ttcgccggaa acagcacagc gcccgctagt ttccgtgcgc gtagccgcca 4500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag 4560

4501 ctccaacggc tccgcgaccg gcccatgctc gacgggtaag aactcagggc atagtgcgtc 4560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag 4620

4561 gcgcactcga tgggtccgtg acggcggcgg ccgtgttggc aagaacttag tcttgggctc 4620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga 4680

4621 ccgctgcgac gggcgctcca ggtccgcgac cggcgacttt aatttagttt tgagtaaact 4680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga 4740

4681 caattactcc atttctcttt tactcgtttt cgtgtttgtg cgattcacgg ccggcaggct 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc 4800

4741 cgcgtgcgtc gtcgttccga cgttgcaacc ggtcggaccg tctgtgcggt cggtacttcg 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc 4860

4801 cccagttgaa agtcaacggc cgcctcctag tgtggttcga cttctacatg cgccatgcgg 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga 4920

4861 ttccgttctg gtaatggctc gacgatagac ttatgtagcg cgtcgatggt ctcatttact 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa 4980

4921 cgtttactta tttactcatc tacttaaaat cgccgatttc ctccgccgta ccttttagtt 4980

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

4981 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca 5040

4981 cttgttggtc cgtggctgcg gcaccttacg gggtacacac ctccttgccc gccaaccggt 5040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag 5100

5041 ccgcattcgc cgacccaaca gacggccggg acgttaccgt gaccttgggg gttcgggctc 5100

.......... .......... ORF 2 .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

5101 gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 5160

5101 cttagccgca ctgccagcgt ttggtaggcc gggccatgtt tagccgcgcc gcgacccact 5160

.......... ......... ORF 2 ORF 2 ......... .......... ..........

SexAI(5164) ........ .......... .......... .......... ..........

5161 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga 5220

5161 actggaccac ctcttcaact tccggcgcgt ccggcgggtc gccgttgcgt agctccgtct 5220

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .... NotI(5248),EagI(5248) .... .......... ..........

5221 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 5280

5221 tcgtgcgggg ccacttagca ccgttcgccg gcgactagct taggcgtttc ttagggccgt 5280

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 5340

5281 tggcggccgt cggccacgcg gcagctaatc cttcggcggg ttcccgctgc tcgttggtct 5340

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 5400

5341 aaaaaagcaa ggctacgaga tactgcaccc gtgggcgcta tcagcgtcgt agtacctgca 5400

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 5460

5401 ccggcaaaag gcagacagct tcgcactggc tgctcgaccg ctccactagg cgatgctcga 5460

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... EagI(5494) .......... ..........

5461 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta 5520

5461 aggtctgccc gtgcatctcc aaaggcgtcc cggccggccg taccggtcac acaccctaat 5520

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 5580

5521 gctggaccat gactaccgcc aaagggtaga ttggcttagg tacttggcta tggcccttcc 5580

.......... ORF 2 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 5640

5581 cttccctctg ttcgggccgg cgcacaaggc aggtgtgcaa cgcctgcatg agttcaagac 5640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 5700

5641 ggccgctcgg ctaccgcctt tcgtctttct gctggaccat ctttggacgt aagccaattt 5700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 5760

5701 gtggtgcgtg caacggtacg tcgcatgctt cttccggttc ttgccggcgg accactgcca 5760

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 5820

5761 taggctccca cttcggaact aatcggcgat gttctagcat ttctcgcttt ggcccgccgg 5820

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 5880

5821 cctcatgtag ctctagctcg atcgactaac ctacatggcg ctctagtgtc ttccgttctt 5880

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... ClaI(5920) .......... EagI(5933)

5881 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 5940

5881 gggcctgcac gactgccaag tggggctaat gaaaaactag ctagggccgt agccggcaaa 5940

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 6000

5941 agagatggcg gaccgtgcgg cgcggcgtcc gttccgtctt cggtctacca acaagttctg 6000

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 6060

6001 ctagatgctt gcgtcaccgt cgcggcctct caagttcttc aagacaaagt ggcacgcgtt 6060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 6120

6061 cgactagccc agtttactgg acggcctcat gctaaacttc ctcctccgcc ccgtccgacc 6120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 6180

6121 gggctaggat cagtacgcga tggcgttgga ctagctcccg cttcgtaggc ggccaaggat 6180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 6240

6181 tacatgcctc gtctacgatc ccgtttaacg ggatcgtccc ctttttccag cttttccaga 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 6300

6241 gaaaggacac ctatcgtgca tgtaaccctt gggtttcggc atgtaaccct tggccttggg 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 6360

6301 catgtaaccc ttgggtttcg gcatgtaacc cttggccagt gtgtacattc actgactata 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 6420

6361 ttttctcttt tttccgctaa aaaggcggat tttgagaaat tttgaataat tttgagaatt 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 6480

6421 ttgggcggac cggacacgta ttgacagacc ggtcgcgtgt cggcttctcg acgtttttcg 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 6540

6481 cggatgggaa gccagcgacg cgagggatgc ggggcggcga agcgcagccg gatagcgccg 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 6600

6541 gcgaccggcg agtttttacc gaccggatgc cggtccgtta gatggtcccg cgcctgttcg 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg 6660

6601 gcgcggcagc ggtgagctgg cggccgcggg tgtagttccg tgggacggag cgcgcaaagc 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 6720

6661 cactactgcc acttttggag actgtgtacg tcgagggcct ctgccagtgt cgaacagaca 6720

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6721 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 6780

6721 ttcgcctacg gccctcgtct gttcgggcag tcccgcgcag tcgcccacaa ccgcccacag 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 6840

6781 ccccgcgtcg gtactgggtc agtgcatcgc tatcgcctca catatgaccg aattgatacg 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NdeI(6874) .......... ..........

6841 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 6900

6841 ccgtagtctc gtctaacatg actctcacgt ggtatacgcc acactttatg gcgtgtctac 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 6960

6901 gcattcctct tttatggcgt agtccgcgag aaggcgaagg agcgagtgac tgagcgacgc 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7020

6961 gagccagcaa gccgacgccg ctcgccatag tcgagtgagt ttccgccatt atgccaatag 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7080

7021 gtgtcttagt cccctattgc gtcctttctt gtacactcgt tttccggtcg ttttccggtc 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7140

7081 cttggcattt ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg gactgctcgt 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7200

7141 agtgttttta gctgcgagtt cagtctccac cgctttgggc tgtcctgata tttctatggt 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7260

7201 ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa ggctgggacg gcgaatggcc 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7320

7261 tatggacagg cggaaagagg gaagcccttc gcaccgcgaa agagtatcga gtgcgacatc 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7380

7321 catagagtca agccacatcc agcaagcgag gttcgacccg acacacgtgc ttggggggca 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7440

7381 agtcgggctg gcgacgcgga ataggccatt gatagcagaa ctcaggttgg gccattctgt 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7500

7441 gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct ccatacatcc 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7560

7501 gccacgatgt ctcaagaact tcaccaccgg attgatgccg atgtgatctt cctgtcataa 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7620

7561 accatagacg cgagacgact tcggtcaatg gaagcctttt tctcaaccat cgagaactag 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7680

7621 gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg tctaatgcgc 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7740

7681 gtcttttttt cctagagttc ttctaggaaa ctagaaaaga tgccccagac tgcgagtcac 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc 7800

7741 cttgcttttg agtgcaattc cctaaaacca gtacgtaaga tccatgattt tgttaagtag 7800

.......... .......... .......... .......... ......... KanR1 ORF 3

.......... .......... .......... .......... .......... ..........

7801 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa 7860

7801 gtcattttat attataaaat aaaagagggt tagtccgaac taggggtcat tcagtttttt 7860

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... ......... EcoRV(7888) ......... .......... ..........

7861 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat 7920

7861 atcgagctgt atgacaagaa ggggctatag gagggactag ctggcctgcg tcttccgtta 7920

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc 7980

7921 cagtatggtg aacaggcggg acggcgaaga gggttctagt tatttcggtg aatgaaacgg 7980

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc 8040

7981 tagaaagtgt ttctacaacg acagagggtc cagcggcacc cttttctgtt caaggagaag 8040

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc 8100

8041 cccgaaaagg cagaaatttt ttagtatgtc gagcgcgcct agaaatttac ctcacagaag 8100

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc 8160

8101 aagggtcaaa agcgttaggt gtagccggtc tagcaataag tcattcatta ggttaagccg 8160

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag 8220

8161 attcgccgac agattcgata agcatatccc tgttaggcta tacagctacc tcactttctc 8220

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc 8280

8221 ggactacgtg aggcgtatgt cgagctatta gaaaagtccc gaaacaagta gaagtatgag 8280

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg 8340

8281 aaggctcgtt tcctgcggta gccggagtga gtactcgtct aacgaggtcg gtagtacggc 8340

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt 8400

8341 aagtttcacg tcctggaaac cttgtccgtc gaaaggaagg tcggtatcgt agtacaggaa 8400

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag 8460

8401 aagggcaagg tgtagtatcc accagggaaa tatggccgac aggcagtaaa aatttatatc 8460

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt 8520

8461 caaaagtaaa agagggtggt cgaatatatg gaatcgtcct ctgtaaggaa ggcatagaaa 8520

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat 8580

8521 atgcgtcgcc ataaaaagct agtcaaaaaa gttaaggcca ctataagagt aaaatcggta 8580

.......... ......... KanR1 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac 8640

8581 aataataaag gaaggagaaa agatgtcata aatttctatg gggttcttcg attaatattg 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct 8700

8641 ttctgcttga ggttaagtga caaggaacgt aagattttgg aatttatggt cttttgtcga 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa 8760

8701 aaaagtttca acaaaagttt caaccgcata ttgtatcata gctgcctcgg ctaaaacttt 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc 8820

8761 ggcgccacta gtgtccgtcg ttgcgagaca gtagcaatgt tagttgtacg atgggaggcg 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta 8880

8821 ctctagtagg cacaaagttt gggccgtcga atcaacggca agaaggctta tcgtagccat 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg 8940

8881 tgtactcgtt tcagacggcg gaatgttgcc gagagggcga ctgcggcagg gcctgactac 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg 9000

8941 ccgacggaca tagctcacca ctaaaacacg gctcgacggc cagcccctcg acaaccgacc 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat 9060

9001 gaccaccgtc ctatataaca ccacatttgt ttaactgcga atctgttgaa ttattgtgta 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag 9120

9061 acgcctgcaa aaattacatg acttaattgc ggcttaatta agccccctag acctaaaatc 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 tactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa 9180

9121 atgacctaaa accaaaatcc ttaatcttta aaataactat cttcataaaa tgtttatgtt 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa 9240

9181 tatgtatgat tcccaaagaa tatacgagtt gtgtactcgc tttgggatat ccttgggatt 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac 9300

9241 aagggaatag acccttgatg agtgtgtaat aatacctctt tgagctcgaa cagctagctg 9300

.......... .......... .......... .......... ORF 4 ..........

.......... .......... .......... .......... .......... ..........

9301 agatccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg gcgtcggttt 9360

9301 tctaggccag ccgtagatga gataaagaaa cgggagcctg ctcacgaccc cgcagccaaa 9360

.......... .......... ORF 4 ORF 1 .......... ..........

.......... ScaI(9376) .......... .......... EagI(9402) ..........

9361 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg 9420

9361 ggtgatagcc gctcatgaag atgtgtcggt agccaggtct gccggcgcga agacgcccgc 9420

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc 9480

9421 taaacacatg cgggctgtca gggccgaggc ctagcctgct aacgcagcgt agctgggacg 9480

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca 9540

9481 cgggttcgac gtagtagctt taacggcagt tggttcgaga ctatctcaac cagttctggt 9540

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg cctccgctcg 9600

9541 tacgcctcgt atatgcgggc ctcagcaccg ctaggacgtt cgaggcctac ggaggcgagc 9600

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg 9660

9601 ttcatcgcgc agacgacgag gtatgttcgg ttggtgccgg aggtcttctt ctacaaccgc 9660

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg 9720

9661 tggagcataa cccttagggg cttgtagcgg agcgaggtca gttactggcg acaatacgcc 9720

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg 9780

9721 ggtaacaggc agtcctgtaa caacctcggc tttaggcgca cgtgctccac ggcctgaagc 9780

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg 9840

9781 cccgtcagga gccgggtttc gtagtcgagt agctctcgga cgcgctgcct gcgtgactgc 9840

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa 9900

9841 cacagcaggt agtgtcaaac ggtcactatg tgtaccccta gtcgttagcg cgtatacttt 9900

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc 9960

9901 agtgcggtac atcacataac tggctaagga acgccaggct tacccggctt gggcgagcag 9960

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg tagaacagcg 10020

9961 accgattcta gccggcgtcg ctagcgtagg tatcggaggc gctggccaac atcttgtcgc 10020

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa 10080

10021 ccgtcaagcc aaagtccgtc cagaacgttg cactgtggga cacgtgccgc cctctacgtt 10080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc 10140

10081 atccagtccg agagcgattt gaggggttac agttcgtgaa ggccttagcc ctcgcgccgg 10140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc 10200

10141 ctacgtttca cggctatttg tattgctaga aacatctttg gtagccgcgt cgataaatgg 10200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc 10260

10201 gcgtcctgta taggtgcggg aggatgtagc ttcgactttc gtgctctaag aagcgggagg 10260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac 10320

10261 ctctcgacgt agtccagcct ctgcgacagc ttgaaaagct agtctttgaa gagctgtctg 10320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... ... XmaI(10360),SmaI(10362) ...

10321 gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc gaaagctcga 10380

10321 cagcgccact caagtccgaa aaagtataga gtaacggggg gccctagacg ctttcgagct 10380

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga 10440

10381 ctctctatct aaacatctct ctctgaccac taaagtcgca caggagaggt ttactttact 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg 10500

10441 tgaaggaata tatctccttc cagaacgctt cctatcaccc taacacgcag tagggaatgc 10500

.......... .......... .......... .......... .......... ..........

......... EcoRV(10512) ......... .......... .......... ..........

10501 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt 10560

10501 agtcacctct atagtgtagt taggtgaacg aaacttctgc accaaccttg cagaagaaaa 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct 10620

10561 aggtgctacg aggagcaccc acccccaggt agaaaccctg gtgacagccg tctccgtaga 10620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct 10680

10621 acttgctatc ggaaaggaaa tagcgttact accgtaaaca tccacggtgg aaggaaaaga 10680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat 10740

10681 tgacaggaaa actacttcac tgtctatcga cccgttacct taggctcctc caaagggcta 10740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata 10800

10741 taatgggaaa caacttttca gagttatcgg gaaaccagaa gactctgaca tagaaactat 10800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt 10860

10801 aagaacctca tctgctctca cagcacgagg tggtacaata gtgtagttag gtgaacgaaa 10860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct 10920

10861 cttctgcacc aaccttgcag aagaaaaagg tgctacgagg agcacccacc cccaggtaga 10920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg 10980

10921 aaccctggtg acagccgtct ccgtagaact tgctatcgga aaggaaatag cgttactacc 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg 11040

10981 gtaaacatcc acggtggaag gaaaagatga caggaaaact acttcactgt ctatcgaccc 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt 11100

11041 gttaccttag gctcctccaa agggctataa tgggaaacaa cttttcagag ttatcgggaa 11100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11101 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc 11160

11101 accagaagac tctgacatag aaactataag aacctcatct gctctcacag cacgaggtgg 11160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11161 atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 11220

11161 tacaaccgtt cgacgagatc ggttatgcgt ttggcggaga ggggcgcgca accggctaag 11220

.......... .......... .......... .......... .......... ..........

......... PvuII(11231) ......... .......... .......... ..........

11221 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 11280

11221 taattacgtc gaccgtgctg tccaaagggc tgacctttcg cccgtcactc gcgttgcgtt 11280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 11340

11281 aattacactc aatcgagtga gtaatccgtg gggtccgaaa tgtgaaatac gaaggccgag 11340

.......... .......... .......... ......... lac_promoter .........

.......... .......... .......... .......... .......... ..........

11341 gtatgttgtg tggaattgtg agcggataac aattt..... .......... .......... 11375

11341 catacaacac accttaacac tcgcctattg ttaaa..... .......... .......... 11375

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-11375 Reference author: Shi X.G., Zhuang W.J.; Reference title: "Binary vector pCAMBIA1300-TMT-2S for making gamma-tocopherol Reference title: methyltransferase over expression in seed"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-11375 Reference author: Zhuang W.J., Shi X.G.; Reference title: ; Reference location: Submitted (08-OCT-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Crop Science Department, Oil Crop Institution, Fujian Agriculture, Jinshan, Reference location: Fuzhou, Fujian 350002, P.R. China