yEyEDID FJ362598; SV 1; circular; other DNA; STD; SYN; 11288 BP. AC FJ362598; DT 16-DEC-2008 (Rel. 98, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 1) DE Binary vector pCAMBIA1300-VE2-7S, complete sequence. KW . OS Binary vector pCAMBIA1300-VE2-7S OC other sequences; artificial sequences; vectors. RN [1] RP 1-11288 RA Shi X.G., Zhuang W.J.; RT "Binary vector pCAMBIA1300-VE2-7S for making homogentisate RT phytyltransferase over expression in seed"; RL Unpublished. RN [2] RP 1-11288 RA Zhuang W.J., Shi X.G.; RT ; RL Submitted (06-OCT-2008) to the EMBL/GenBank/DDBJ databases. RL Crop Science Department, Oil Crop Institution, Fujian Agriculture, Jinshan, RL Fuzhou, Fujian 350002, P.R. China FH Key Location/Qualifiers FT source 1..11288 FT /organism="Binary vector pCAMBIA1300-VE2-7S" FT /mol_type="other DNA" FT /db_xref="taxon:571926" FT terminator 8..260 FT /note="NOS" FT gene complement(281..1462) FT /gene="HPT1" FT CDS complement(281..1462) FT /codon_start=1 FT /transl_table=11 FT /gene="HPT1" FT /product="homogentisate phytyltransferase 1" FT /note="derived from Arabidopsis thaliana" FT /protein_id="ACJ54263.1" FT /translation="MEFLLSSFFLVSAAGGFCWKKQNLKLHSLSEIRVLRCDSSKVVAK FT PKFRNNLVRPDGQGFSLLLYPKHKWRFRVNATAGQPEAFDSNSKQKFFRDSLDAFYRFF FT RPHTVIGTVLSILSVSFLAVEKVFDIFPLLFTGILEAVVAALMMNIYIVGLNQLFDVEI FT DKVNKPYFPLASGEYSVNTGIAIVASFSIMSFWLGWIVGSWPLFWALFVSFMLGTAYSI FT NLPLLRWKRFALVAAMCILAVRAIIVQIAFYLHIQTHVFGRPILFTRPLIFATAFMSFF FT SVVIALFKDIPDIEGDKIFGIRSFSVTLGQKRVFWTCVTLLQMAYAVAILVGATSPFIW FT SKVISVVGHVILATTLWARAKSVDLSSKTEITSCYMFIWKLFYAEYLLLPFLK" FT promoter 1474..2350 FT /note="soybean 7S promoter" FT misc_feature 2628..2653 FT /note="right border T-DNA repeat" FT CDS complement(7668..8462) FT /codon_start=1 FT /transl_table=11 FT /product="aminoglycoside phosphotransferase" FT /protein_id="ACJ54264.1" FT /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM FT TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED FT EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT FT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FT FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" FT misc_feature 8887..8912 FT /note="left border C58 T-DNA repeat" FT misc_feature 8979..9187 FT /note="CaMV polyA signal" SQ Sequence 11288 BP; 2826 A; 2910 C; 2917 G; 2635 T; 0 other; aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240 tgccaaatgt ttgaacgatc ggggaaattc gagctctcag tcacttcaaa aaaggtaaca 300 gcaagtactc tgcataaaag agcttccata tgaacatata acatgaagtt atttcggttt 360 tgctactcag atcaacggac ttagctcgag cccacaaagt tgttgcgagt ataacatgac 420 ccacaaccga gatgactttg ctccatatga atggagatgt ggctccaact agaattgcaa 480 cagcgtaagc catttgaagt agtgtaacac atgtccaaaa cacccgtttc tgacccagag 540 ttacagagaa tgatcggatt ccgaatatct tatccccttc gatatcaggt atatccttaa 600 acaatgcaat aacgacagag aaaaagctca taaacgcagt ggcgaaaata agaggcctag 660 tgaacaagat tggtcttcca aacacatgtg tctgaatatg tagataaaag gcgatttgaa 720 caataatagc tcggacagcg aggatacaca ttgctgcaac caatgcaaat cttttccacc 780 gtaaaagtgg caaattgata gagtatgcag taccgagcat gaaactcaca aaaagagccc 840 agaacaatgg ccatgaacca acaatccacc caagccagaa actcatgatg gagaaggaag 900 ctactattgc aatgccggtg ttaacagaat attctcctga tgccaatgga aaatagggct 960 tgttaacctt atctatttca acatcaaaca actgatttag cccaactatg taaatgttca 1020 tcatgagagc tgcaacaaca gcctccaaga tgccagtgaa aagtaaagga aatatatcaa 1080 aaaccttctc tactgctaaa aaagatacag ataaaatgct aagcactgtg ccaataactg 1140 tatgaggcct aaaaaacctg taaaacgcat ctaacgagtc tctaaaaaac ttctgtttgc 1200 tattcgagtc aaaagcctca ggctgacccg cagtggcatt aacccgaaat ctccacttat 1260 gttttggata caacaacaat gaaaatcctt gaccatcagg cctaacaaga ttgttcctaa 1320 acttcggttt tgcgacaact ttactcgaat cacaacgcaa aactcggatt tctgataaag 1380 agtggagctt tagattctgc ttcttccaac aaaacccacc agcagcggaa acaagaaaaa 1440 aactagagag cagaaactcc atggtgaccc gggagtatct taaattattt attaaggtat 1500 actaggatat tgaactagtt ctcgatgatg aaaacctgga ccgagattgc gggtatttat 1560 agtcatagaa gagatatagc tctgttaact tgcatgcatg gggtggcatg caagttgacg 1620 ggtgttgaat tgaacaaaat tgatgtgacg gaagatggtt gtgatcgagt tagagtcgca 1680 taggcagtgt acttagaaat taacaagaaa tgtgttgagc gatggtgagc aatttgggct 1740 gcatgttgag tacgtgttga tgtgcatggc atttagacct acatggacgc ggcgatcttg 1800 atcagccatg gatagtgaag tggggtgagg ttgcattttg tctcaatttt atttgatgga 1860 cttggacatt gctttcgaaa ggatacttta tgtgttgtct tttggttgcg catgcatgat 1920 ccaagagact agatagtgtg atatgctgat atggttgctt tccaaagaga acaccgcgtt 1980 tgagatttgg ttacaaacaa caaatgtgtg ggctcatcat ggattgagtt ggtgaatgtt 2040 tttgggcagc acgattgcac cttggtgagt acgtgttgtt gtgcatggct tttgggtcca 2100 ctgtgtgtga ttgcgttttt gtttgtattt tatgaactta gacatttttt tgcaaaggat 2160 actctttatg ttgtcttttt tgttctttgg ctttttctca tgatccaaga gactagtcag 2220 tgttgtggtg gcattcgaga gtaccaagat taaaagaacc gacatgcata taattacgat 2280 ggaagaagca taagtaattg atcggtacgg ctgttatcaa attaattaat gagcggcaaa 2340 tggaggacca ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg 2400 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 2460 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 2520 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt 2580 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag 2640 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg 2700 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca 2760 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa 2820 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg 2880 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca 2940 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga 3000 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt 3060 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca 3120 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg 3180 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc 3240 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg 3300 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc 3360 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca 3420 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg 3480 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga 3540 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc 3600 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa 3660 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag 3720 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc 3780 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa 3840 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga 3900 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga 3960 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg 4020 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg 4080 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg 4140 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact 4200 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt 4260 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg 4320 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg 4380 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc 4440 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg 4500 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt 4560 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc 4620 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa 4680 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg 4740 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat 4800 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc 4860 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc 4920 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg 4980 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt 5040 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca 5100 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg 5160 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca 5220 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac 5280 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag 5340 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat 5400 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa 5460 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc 5520 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta 5580 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg 5640 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg 5700 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag 5760 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt 5820 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag 5880 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc 5940 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct 6000 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc 6060 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt 6120 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac 6180 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat 6240 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt 6300 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa 6360 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg 6420 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa 6480 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt 6540 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 6600 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 6660 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 6720 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 6780 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 6840 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 6900 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6960 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7020 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7080 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7140 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7200 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7260 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7320 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7380 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 7440 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7500 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7560 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 7620 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca 7680 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa 7740 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca 7800 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg 7860 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct 7920 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct 7980 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg 8040 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag 8100 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac 8160 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc 8220 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc 8280 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat 8340 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct 8400 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc 8460 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata 8520 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag 8580 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga 8640 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc 8700 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg 8760 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga 8820 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct 8880 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac 8940 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt 9000 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac 9060 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct 9120 aattccctta tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg 9180 acagatccgg tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt 9240 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg 9300 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct 9360 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac 9420 caatgcggag catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct 9480 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg 9540 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc 9600 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt 9660 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga 9720 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga 9780 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg 9840 tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag 9900 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc 9960 aataggtcag gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg 10020 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta 10080 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct 10140 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag 10200 acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct gcgaaagctc 10260 gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat 10320 gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta 10380 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 10440 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 10500 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt 10560 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg 10620 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga 10680 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct 10740 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 10800 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 10860 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 10920 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 10980 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 11040 ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat 11100 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 11160 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 11220 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 11280 tgattacg 11288 ,pCAMBIA1300-VE2-7S"Times New Roman yEEEcoRI(1),SacI(276),ScaI(307),NdeI(329),XhoI(386),NdeI(446),EcoRV(584) ArialI,|rh$ h yEWHpaI(923),HpaI(965),Bsu36I(1218),NcoI(1460),XmaI(1469),SmaI(1471),SpeI(1515),HpaI(1587) Arial,[biiQ yErBclI(1800),NcoI(1807),SpeI(2213),PacI(2327),BamHI(2352),XbaI(2358),SalI(2364),PstI(2374),HindIII(2382),PvuII(2477) Arial0 i,# X yE6PmeI(2595),ScaI(2750),EagI(3040),EagI(3268),EagI(3272) Arial ,wxy[ yELSfiI(3363),NotI(3598),EagI(3598),EagI(3702),SfiI(3797),SfiI(3806),EagI(4076) Arialv , yE,EagI(4152),NdeI(4267),SexAI(4283),EagI(4613) Arial,U\ bbB yE͆SexAI(5046),SfiI(5072),EagI(5130),NotI(5130),EagI(5376),SfiI(5384),SexAI(5406),EagI(5479),SexAI(5556),EagI(5626),EagI(5699),NheI(5722) Arial,BXjj$ yE+ClaI(5802),EagI(5815),EagI(6420),NotI(6420) Arial,DNYbY yENdeI Ariald,2,#&++&7 yEEcoRV ArialZ, yE6BclI(8650),ScaI(9004),XhoI(9165),ScaI(9258),EagI(9284) Arial#, yERNdeI(9776),EagI(9854),EagI(10019),XmaI(10242),SmaI(10244),XhoI(10259),EcoRV(10394) Arial(,5/);) yEPvuII Ariali+,zpgU[gyEC,HPT1"Times New RomanyEC,V f M13_forward20_primer"Times New RomanyEC,_ u M13_pUC_fwd_primer"Times New RomanyEC, 2ORF 2"Times New RomanyEC,]ORF 4"Times New RomanyE,6ORF 4"Times New RomanyECɆ,]ORF 1"Times New RomanyECɆ, pGEX_3_primer"Times New RomanyE,!KanR1"Times New RomanyEC,!ORF 1"Times New RomanyEC,#&ORF 3"Times New RomanyEC,#'ORF 2"Times New RomanyEC,++ lac_promoter"Times New RomanyEC,+,M13_pUC_rev_primer"Times New RomanyECɆ,,,M13_reverse_primer"Times New Roman yEtest"Times New Romane 1R,S'&d-d; yEtest"Times New Roman+,yP)