Sequence of pCAMBIA1300-VE2-7S

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Sequence of pCAMBIA1300-VE2-7S

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Sequence of pCAMBIA1300-VE2-7S: 11288 BP; 2826 A; 2910 C; 2917 G; 2635 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60

1 ttaagggcta gatcattgta tctactgtgg cgcgcgctat taaataggat caaacgcgcg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120

61 atataaaaca aaagatagcg cataatttac atattaacgc cctgagatta gtatttttgg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180

121 gtagagtatt tattgcagta cgtaatgtac aattaataat gtacgaattg cattaagttg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240

181 tctttaatat actattagta gcgttctggc cgttgtccta agttagaatt ctttgaaata 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgccaaatgt ttgaacgatc ggggaaattc gagctctcag tcacttcaaa aaaggtaaca 300

241 acggtttaca aacttgctag cccctttaag ctcgagagtc agtgaagttt tttccattgt 300

.......... .......... .......... .......... HPT1 ..........

ScaI(307) .......... .......... .......... .......... ..........

301 gcaagtactc tgcataaaag agcttccata tgaacatata acatgaagtt atttcggttt 360

301 cgttcatgag acgtattttc tcgaaggtat acttgtatat tgtacttcaa taaagccaaa 360

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgctactcag atcaacggac ttagctcgag cccacaaagt tgttgcgagt ataacatgac 420

361 acgatgagtc tagttgcctg aatcgagctc gggtgtttca acaacgctca tattgtactg 420

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccacaaccga gatgactttg ctccatatga atggagatgt ggctccaact agaattgcaa 480

421 ggtgttggct ctactgaaac gaggtatact tacctctaca ccgaggttga tcttaacgtt 480

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cagcgtaagc catttgaagt agtgtaacac atgtccaaaa cacccgtttc tgacccagag 540

481 gtcgcattcg gtaaacttca tcacattgtg tacaggtttt gtgggcaaag actgggtctc 540

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... EcoRV(584) ..........

541 ttacagagaa tgatcggatt ccgaatatct tatccccttc gatatcaggt atatccttaa 600

541 aatgtctctt actagcctaa ggcttataga ataggggaag ctatagtcca tataggaatt 600

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acaatgcaat aacgacagag aaaaagctca taaacgcagt ggcgaaaata agaggcctag 660

601 tgttacgtta ttgctgtctc tttttcgagt atttgcgtca ccgcttttat tctccggatc 660

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgaacaagat tggtcttcca aacacatgtg tctgaatatg tagataaaag gcgatttgaa 720

661 acttgttcta accagaaggt ttgtgtacac agacttatac atctattttc cgctaaactt 720

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 caataatagc tcggacagcg aggatacaca ttgctgcaac caatgcaaat cttttccacc 780

721 gttattatcg agcctgtcgc tcctatgtgt aacgacgttg gttacgttta gaaaaggtgg 780

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtaaaagtgg caaattgata gagtatgcag taccgagcat gaaactcaca aaaagagccc 840

781 cattttcacc gtttaactat ctcatacgtc atggctcgta ctttgagtgt ttttctcggg 840

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agaacaatgg ccatgaacca acaatccacc caagccagaa actcatgatg gagaaggaag 900

841 tcttgttacc ggtacttggt tgttaggtgg gttcggtctt tgagtactac ctcttccttc 900

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctactattgc aatgccggtg ttaacagaat attctcctga tgccaatgga aaatagggct 960

901 gatgataacg ttacggccac aattgtctta taagaggact acggttacct tttatcccga 960

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgttaacctt atctatttca acatcaaaca actgatttag cccaactatg taaatgttca 1020

961 acaattggaa tagataaagt tgtagtttgt tgactaaatc gggttgatac atttacaagt 1020

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcatgagagc tgcaacaaca gcctccaaga tgccagtgaa aagtaaagga aatatatcaa 1080

1021 agtactctcg acgttgttgt cggaggttct acggtcactt ttcatttcct ttatatagtt 1080

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaaccttctc tactgctaaa aaagatacag ataaaatgct aagcactgtg ccaataactg 1140

1081 tttggaagag atgacgattt tttctatgtc tattttacga ttcgtgacac ggttattgac 1140

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tatgaggcct aaaaaacctg taaaacgcat ctaacgagtc tctaaaaaac ttctgtttgc 1200

1141 atactccgga ttttttggac attttgcgta gattgctcag agattttttg aagacaaacg 1200

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tattcgagtc aaaagcctca ggctgacccg cagtggcatt aacccgaaat ctccacttat 1260

1201 ataagctcag ttttcggagt ccgactgggc gtcaccgtaa ttgggcttta gaggtgaata 1260

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttttggata caacaacaat gaaaatcctt gaccatcagg cctaacaaga ttgttcctaa 1320

1261 caaaacctat gttgttgtta cttttaggaa ctggtagtcc ggattgttct aacaaggatt 1320

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acttcggttt tgcgacaact ttactcgaat cacaacgcaa aactcggatt tctgataaag 1380

1321 tgaagccaaa acgctgttga aatgagctta gtgttgcgtt ttgagcctaa agactatttc 1380

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agtggagctt tagattctgc ttcttccaac aaaacccacc agcagcggaa acaagaaaaa 1440

1381 tcacctcgaa atctaagacg aagaaggttg ttttgggtgg tcgtcgcctt tgttcttttt 1440

.......... .......... HPT1 .......... .......... ..........

.......... NcoI(1460) .......... .......... .......... ..........

1441 aactagagag cagaaactcc atggtgaccc gggagtatct taaattattt attaaggtat 1500

1441 ttgatctctc gtctttgagg taccactggg ccctcataga atttaataaa taattccata 1500

.......... HPT1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 actaggatat tgaactagtt ctcgatgatg aaaacctgga ccgagattgc gggtatttat 1560

1501 tgatcctata acttgatcaa gagctactac ttttggacct ggctctaacg cccataaata 1560

.......... .......... .......... .......... .......... ..........

.......... .......... HpaI(1587) .......... .......... ..........

1561 agtcatagaa gagatatagc tctgttaact tgcatgcatg gggtggcatg caagttgacg 1620

1561 tcagtatctt ctctatatcg agacaattga acgtacgtac cccaccgtac gttcaactgc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggtgttgaat tgaacaaaat tgatgtgacg gaagatggtt gtgatcgagt tagagtcgca 1680

1621 ccacaactta acttgtttta actacactgc cttctaccaa cactagctca atctcagcgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 taggcagtgt acttagaaat taacaagaaa tgtgttgagc gatggtgagc aatttgggct 1740

1681 atccgtcaca tgaatcttta attgttcttt acacaactcg ctaccactcg ttaaacccga 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcatgttgag tacgtgttga tgtgcatggc atttagacct acatggacgc ggcgatcttg 1800

1741 cgtacaactc atgcacaact acacgtaccg taaatctgga tgtacctgcg ccgctagaac 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcagccatg gatagtgaag tggggtgagg ttgcattttg tctcaatttt atttgatgga 1860

1801 tagtcggtac ctatcacttc accccactcc aacgtaaaac agagttaaaa taaactacct 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cttggacatt gctttcgaaa ggatacttta tgtgttgtct tttggttgcg catgcatgat 1920

1861 gaacctgtaa cgaaagcttt cctatgaaat acacaacaga aaaccaacgc gtacgtacta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccaagagact agatagtgtg atatgctgat atggttgctt tccaaagaga acaccgcgtt 1980

1921 ggttctctga tctatcacac tatacgacta taccaacgaa aggtttctct tgtggcgcaa 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgagatttgg ttacaaacaa caaatgtgtg ggctcatcat ggattgagtt ggtgaatgtt 2040

1981 actctaaacc aatgtttgtt gtttacacac ccgagtagta cctaactcaa ccacttacaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttgggcagc acgattgcac cttggtgagt acgtgttgtt gtgcatggct tttgggtcca 2100

2041 aaacccgtcg tgctaacgtg gaaccactca tgcacaacaa cacgtaccga aaacccaggt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctgtgtgtga ttgcgttttt gtttgtattt tatgaactta gacatttttt tgcaaaggat 2160

2101 gacacacact aacgcaaaaa caaacataaa atacttgaat ctgtaaaaaa acgtttccta 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actctttatg ttgtcttttt tgttctttgg ctttttctca tgatccaaga gactagtcag 2220

2161 tgagaaatac aacagaaaaa acaagaaacc gaaaaagagt actaggttct ctgatcagtc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgttgtggtg gcattcgaga gtaccaagat taaaagaacc gacatgcata taattacgat 2280

2221 acaacaccac cgtaagctct catggttcta attttcttgg ctgtacgtat attaatgcta 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggaagaagca taagtaattg atcggtacgg ctgttatcaa attaattaat gagcggcaaa 2340

2281 ccttcttcgt attcattaac tagccatgcc gacaatagtt taattaatta ctcgccgttt 2340

.......... .......... .......... .......... .......... ..........

.... BamHI(2352) XbaI(2358) SalI(2364) PstI(2374) .... ..........

2341 tggaggacca ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg 2400

2341 acctcctggt cctaggagat ctcagctgga cgtccgtacg ttcgaaccgt gaccggcagc 2400

.......... .......... .......... .......... ..... M13_forward20_primer

.......... .......... .......... .......... .......... ..........

2401 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 2460

2401 aaaatgttgc agcactgacc cttttgggac cgcaatgggt tgaattagcg gaacgtcgtg 2460

...... M13_pUC_fwd_primer ...... .......... .......... ..........

......... PvuII(2477) ......... .......... .......... ..........

2461 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 2520

2461 tagggggaaa gcggtcgacc gcattatcgc ttctccgggc gtggctagcg ggaagggttg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt 2580

2521 tcaacgcgtc ggacttaccg cttacgatct cgtcgaactc gaacctagtc taacagcaaa 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag 2640

2581 gggcggaagt caaatttgat agtcacaaac tgtcctatat aaccgcccat ttggattctc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg 2700

2641 ttttctcgca aataatctta ttgcctataa attttcccgc acttttccaa ataggcaagc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca 2760

2701 aggtaaacat acacgtacgg ttggtgtccc aaggggagcc ctagtttcat gaaactaggt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa 2820

2761 tggggaggcg acgatatcac gtcagccgaa gactgcaagt cacgtcggca gaagactttt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg 2880

2821 gctgtacagc gtgttcagga ttcaatgcgc tgtccgacgg cgggacggga aaaggaccgc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca 2940

2881 aaaagaacag cgcacaaaat cagcgtattt catcttatga acgctgatct tggcctctgt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga 3000

2941 aatgcggtac ttgttctcgc ggcggcgacc ggacgacccg atacgggcgc agtcgtggct 3000

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt 3060

3001 gctggtcctg aactggttgg ttgcccggct tgacgtgcgc cggccgacgt ggttcgacaa 3060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca 3120

3061 aaggctcttc tagtggccgt ggtccgcgct ggcgggcctc gaccggtcct acgaactggt 3120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg 3180

3121 ggatgcggga ccgctgcaac actgtcactg gtccgatctg gcggaccggg cgtcgtgggc 3180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc 3240

3181 gctggatgac ctgtaacggc tcgcgtaggt cctccggccg cgcccggacg catcggaccg 3240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .... EagI(3268),EagI(3272) .... ..........

3241 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg 3300

3241 tctcggcacc cggctgtggt ggtgcggccg gccggcgtac cacaactggc acaagcggcc 3300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc 3360

3301 gtaacggctc aagctcgcaa gggattagta gctggcgtgg gcctcgcccg cgctccggcg 3360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca 3420

3361 gttccgggct ccgcacttca aaccgggggc gggatgggag tggggccgtg tctagcgcgt 3420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg 3480

3421 gcgggcgctc gactagctgg tccttccggc gtggcacttt ctccgccgac gtgacgaacc 3480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga 3540

3481 gcacgtagcg agctgggaca tggcgcgtga actcgcgtcg ctccttcact gcgggtggct 3540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc 3600

3541 ccggtccgcc gcgccacgga aggcactcct gcgtaactgg ctccggctgc gggaccgccg 3600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa 3660

3601 gcggctctta cttgcggttc tccttgttcg tactttggcg tggtcctgcc ggtcctgctt 3660

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag 3720

3661 ggcaaaaagt aatggcttct ctagctccgc ctctactagc gccggcccat gcacaagctc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc 3780

3721 ggcgggcgcg tgcagagttg gcacgccgac gtactttagg accggccaaa cagactacgg 3780

.......... .......... .......... .......... .......... ..........

.......... SfiI(3797) .......... .......... .......... ..........

3781 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa 3840

3781 ttcgaccgcc ggaccggccg gtcgaaccgg cgacttcttt ggctcgcggc ggcagatttt 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga 3900

3841 tccactacac ataaactcat tttgtcgaac gcagtacgcc agcgacgcat atactacgct 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga 3960

3901 actcatttat ttgtttatgc gttccccttg cgtacttcca atagcgacat gaattggtct 3960

.......... .......... .......... .......... ORF 4 ..........

.......... .......... .......... .......... .......... ..........

3961 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg 4020

3961 ttccgcccag tccgttctgc tggtagcgtt gggtagatcg ggcgcgggac gttgagcggc 4020

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... EagI(4076)

4021 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg 4080

4021 cccggctaca agacaatcag ctaaggctag gggtcccgtc acgggcgcta acccgccggc 4080

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg 4140

4081 acgcccttct agttggcgat tggcaacagc cgtagctggc gggctgctaa ctggcgctgc 4140

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact 4200

4141 acttccggta gccggccgcg ctgaagcatc actagctgcc tcgcggggtc cgccgcctga 4200

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt 4260

4201 accgacacag gcgctagttc cgtcggctga agcacgacta aggccacgtc ggttcgggaa 4260

.......... .......... ORF 4 .......... .......... ..........

NdeI(4267) .......... .......... .......... .......... ..........

4261 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg 4320

4261 tgctgtatac ccggtggcgg ctggaccacc tcgaccaatt cgtcgcgtaa ctccagtgcc 4320

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg 4380

4321 taccttccga tgttcgccgg aaacagcaca gcgcccgcta gtttccgtgc gcgtagccgc 4380

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc 4440

4381 cactccaacg gctccgcgac cggcccatgc tcgacgggta agaactcagg gcatagtgcg 4440

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg 4500

4441 tcgcgcactc gatgggtccg tgacggcggc ggccgtgttg gcaagaactt agtcttgggc 4500

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt 4560

4501 tcccgctgcg acgggcgctc caggtccgcg accggcgact ttaatttagt tttgagtaaa 4560

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... EagI(4613)

4561 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc 4620

4561 ctcaattact ccatttctct tttactcgtt ttcgtgtttg tgcgattcac ggccggcagg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa 4680

4621 ctcgcgtgcg tcgtcgttcc gacgttgcaa ccggtcggac cgtctgtgcg gtcggtactt 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg 4740

4681 cgcccagttg aaagtcaacg gccgcctcct agtgtggttc gacttctaca tgcgccatgc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat 4800

4741 ggttccgttc tggtaatggc tcgacgatag acttatgtag cgcgtcgatg gtctcattta 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc 4860

4801 ctcgtttact tatttactca tctacttaaa atcgccgatt tcctccgccg taccttttag 4860

.......... .......... .......... .......... ORF 4 ..........

.......... .......... .......... .......... .......... ..........

4861 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc 4920

4861 ttcttgttgg tccgtggctg cggcacctta cggggtacac acctccttgc ccgccaaccg 4920

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg 4980

4921 gtccgcattc gccgacccaa cagacggccg ggacgttacc gtgaccttgg gggttcgggc 4980

.......... .......... ORF 4 .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

4981 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt 5040

4981 tccttagccg cactgccagc gtttggtagg ccgggccatg tttagccgcg ccgcgaccca 5040

.......... ......... ORF 4 ORF 1 ......... .......... ..........

SexAI(5046) ........ .......... .......... .......... ..........

5041 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca 5100

5041 ctactggacc acctcttcaa cttccggcgc gtccggcggg tcgccgttgc gtagctccgt 5100

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg 5160

5101 cttcgtgcgg ggccacttag caccgttcgc cggcgactag cttaggcgtt tcttagggcc 5160

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca 5220

5161 gttggcggcc gtcggccacg cggcagctaa tccttcggcg ggttcccgct gctcgttggt 5220

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac 5280

5221 ctaaaaaagc aaggctacga gatactgcac ccgtgggcgc tatcagcgtc gtagtacctg 5280

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag 5340

5281 caccggcaaa aggcagacag cttcgcactg gctgctcgac cgctccacta ggcgatgctc 5340

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... EagI(5376) .......... ..........

5341 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat 5400

5341 gaaggtctgc ccgtgcatct ccaaaggcgt cccggccggc cgtaccggtc acacacccta 5400

.......... ......... ORF 4 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa 5460

5401 atgctggacc atgactaccg ccaaagggta gattggctta ggtacttggc tatggccctt 5460

.......... ORF 4 ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc 5520

5461 cccttccctc tgttcgggcc ggcgcacaag gcaggtgtgc aacgcctgca tgagttcaag 5520

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta 5580

5521 acggccgctc ggctaccgcc tttcgtcttt ctgctggacc atctttggac gtaagccaat 5580

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg 5640

5581 ttgtggtgcg tgcaacggta cgtcgcatgc ttcttccggt tcttgccggc ggaccactgc 5640

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg 5700

5641 cataggctcc cacttcggaa ctaatcggcg atgttctagc atttctcgct ttggcccgcc 5700

.......... .......... ORF 1 .......... .......... ..........

.......... .......... NheI(5722) .......... .......... ..........

5701 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag 5760

5701 ggcctcatgt agctctagct cgatcgacta acctacatgg cgctctagtg tcttccgttc 5760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... ClaI(5802) ..........

5761 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt 5820

5761 ttgggcctgc acgactgcca agtggggcta atgaaaaact agctagggcc gtagccggca 5820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag 5880

5821 aaagagatgg cggaccgtgc ggcgcggcgt ccgttccgtc ttcggtctac caacaagttc 5880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc 5940

5881 tgctagatgc ttgcgtcacc gtcgcggcct ctcaagttct tcaagacaaa gtggcacgcg 5940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct 6000

5941 ttcgactagc ccagtttact ggacggcctc atgctaaact tcctcctccg ccccgtccga 6000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc 6060

6001 ccgggctagg atcagtacgc gatggcgttg gactagctcc cgcttcgtag gcggccaagg 6060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt 6120

6061 attacatgcc tcgtctacga tcccgtttaa cgggatcgtc ccctttttcc agcttttcca 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac 6180

6121 gagaaaggac acctatcgtg catgtaaccc ttgggtttcg gcatgtaacc cttggccttg 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat 6240

6181 ggcatgtaac ccttgggttt cggcatgtaa cccttggcca gtgtgtacat tcactgacta 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt 6300

6241 tattttctct tttttccgct aaaaaggcgg attttgagaa attttgaata attttgagaa 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa 6360

6301 ttttgggcgg accggacacg tattgacaga ccggtcgcgt gtcggcttct cgacgttttt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .... EagI(6420),NotI(6420)

6361 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg 6420

6361 cgcggatggg aagccagcga cgcgagggat gcggggcggc gaagcgcagc cggatagcgc 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa 6480

6421 cggcgaccgg cgagttttta ccgaccggat gccggtccgt tagatggtcc cgcgcctgtt 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt 6540

6481 cggcgcggca gcggtgagct ggcggccgcg ggtgtagttc cgtgggacgg agcgcgcaaa 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 6600

6541 gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt gtcgaacaga 6600

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 6660

6601 cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac aaccgcccac 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 6720

6661 agccccgcgt cggtactggg tcagtgcatc gctatcgcct cacatatgac cgaattgata 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NdeI(6756) .......... ..........

6721 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 6780

6721 cgccgtagtc tcgtctaaca tgactctcac gtggtatacg ccacacttta tggcgtgtct 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 6840

6781 acgcattcct cttttatggc gtagtccgcg agaaggcgaa ggagcgagtg actgagcgac 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 6900

6841 gcgagccagc aagccgacgc cgctcgccat agtcgagtga gtttccgcca ttatgccaat 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6960

6901 aggtgtctta gtcccctatt gcgtcctttc ttgtacactc gttttccggt cgttttccgg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7020

6961 tccttggcat ttttccggcg caacgaccgc aaaaaggtat ccgaggcggg gggactgctc 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7080

7021 gtagtgtttt tagctgcgag ttcagtctcc accgctttgg gctgtcctga tatttctatg 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7140

7081 gtccgcaaag ggggaccttc gagggagcac gcgagaggac aaggctggga cggcgaatgg 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7200

7141 cctatggaca ggcggaaaga gggaagccct tcgcaccgcg aaagagtatc gagtgcgaca 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7260

7201 tccatagagt caagccacat ccagcaagcg aggttcgacc cgacacacgt gcttgggggg 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7320

7261 caagtcgggc tggcgacgcg gaataggcca ttgatagcag aactcaggtt gggccattct 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7380

7321 gtgctgaata gcggtgaccg tcgtcggtga ccattgtcct aatcgtctcg ctccatacat 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 7440

7381 ccgccacgat gtctcaagaa cttcaccacc ggattgatgc cgatgtgatc ttcctgtcat 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7500

7441 aaaccataga cgcgagacga cttcggtcaa tggaagcctt tttctcaacc atcgagaact 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7560

7501 aggccgtttg tttggtggcg accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 7620

7561 gcgtcttttt ttcctagagt tcttctagga aactagaaaa gatgccccag actgcgagtc 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca 7680

7621 accttgcttt tgagtgcaat tccctaaaac cagtacgtaa gatccatgat tttgttaagt 7680

.......... .......... .......... .......... ......... KanR1 ORF 1

.......... .......... .......... .......... .......... ..........

7681 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa 7740

7681 aggtcatttt atattataaa ataaaagagg gttagtccga actaggggtc attcagtttt 7740

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... ......... EcoRV(7770) ......... .......... ..........

7741 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca 7800

7741 ttatcgagct gtatgacaag aaggggctat aggagggact agctggcctg cgtcttccgt 7800

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg 7860

7801 tacagtatgg tgaacaggcg ggacggcgaa gagggttcta gttatttcgg tgaatgaaac 7860

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct 7920

7861 ggtagaaagt gtttctacaa cgacagaggg tccagcggca cccttttctg ttcaaggaga 7920

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct 7980

7921 agcccgaaaa ggcagaaatt ttttagtatg tcgagcgcgc ctagaaattt acctcacaga 7980

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg 8040

7981 agaagggtca aaagcgttag gtgtagccgg tctagcaata agtcattcat taggttaagc 8040

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag 8100

8041 cgattcgccg acagattcga taagcatatc cctgttaggc tatacagcta cctcactttc 8100

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac 8160

8101 tcggactacg tgaggcgtat gtcgagctat tagaaaagtc ccgaaacaag tagaagtatg 8160

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc 8220

8161 agaaggctcg tttcctgcgg tagccggagt gagtactcgt ctaacgaggt cggtagtacg 8220

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc 8280

8221 gcaagtttca cgtcctggaa accttgtccg tcgaaaggaa ggtcggtatc gtagtacagg 8280

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat 8340

8281 aaaagggcaa ggtgtagtat ccaccaggga aatatggccg acaggcagta aaaatttata 8340

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct 8400

8341 tccaaaagta aaagagggtg gtcgaatata tggaatcgtc ctctgtaagg aaggcataga 8400

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc 8460

8401 aaatgcgtcg ccataaaaag ctagtcaaaa aagttaaggc cactataaga gtaaaatcgg 8460

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata 8520

8461 taaataataa aggaaggaga aaagatgtca taaatttcta tggggttctt cgattaatat 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag 8580

8521 tgttctgctt gaggttaagt gacaaggaac gtaagatttt ggaatttatg gtcttttgtc 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga 8640

8581 gaaaaagttt caacaaaagt ttcaaccgca tattgtatca tagctgcctc ggctaaaact 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc 8700

8641 ttggcgccac tagtgtccgt cgttgcgaga cagtagcaat gttagttgta cgatgggagg 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg 8760

8701 cgctctagta ggcacaaagt ttgggccgtc gaatcaacgg caagaaggct tatcgtagcc 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga 8820

8761 attgtactcg tttcagacgg cggaatgttg ccgagagggc gactgcggca gggcctgact 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct 8880

8821 acccgacgga catagctcac cactaaaaca cggctcgacg gccagcccct cgacaaccga 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac 8940

8881 ccgaccaccg tcctatataa caccacattt gtttaactgc gaatctgttg aattattgtg 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt 9000

8941 taacgcctgc aaaaattaca tgacttaatt gcggcttaat taagccccct agacctaaaa 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac 9060

9001 tcatgaccta aaaccaaaat ccttaatctt taaaataact atcttcataa aatgtttatg 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct 9120

9061 tttatgtatg attcccaaag aatatacgag ttgtgtactc gctttgggat atccttggga 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 aattccctta tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg 9180

9121 ttaagggaat agacccttga tgagtgtgta ataatacctc tttgagctcg aacagctagc 9180

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

9181 acagatccgg tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt 9240

9181 tgtctaggcc agccgtagat gagataaaga aacgggagcc tgctcacgac cccgcagcca 9240

.......... .......... ORF 3 .......... ORF 2 ..........

.......... ScaI(9258) .......... .......... EagI(9284) ..........

9241 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg 9300

9241 aaggtgatag ccgctcatga agatgtgtcg gtagccaggt ctgccggcgc gaagacgccc 9300

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct 9360

9301 gctaaacaca tgcgggctgt cagggccgag gcctagcctg ctaacgcagc gtagctggga 9360

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac 9420

9361 cgcgggttcg acgtagtagc tttaacggca gttggttcga gactatctca accagttctg 9420

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 caatgcggag catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct 9480

9421 gttacgcctc gtatatgcgg gcctcagcac cgctaggacg ttcgaggcct acggaggcga 9480

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg 9540

9481 gcttcatcgc gcagacgacg aggtatgttc ggttggtgcc ggaggtcttc ttctacaacc 9540

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc 9600

9541 gctggagcat aacccttagg ggcttgtagc ggagcgaggt cagttactgg cgacaatacg 9600

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt 9660

9601 ccggtaacag gcagtcctgt aacaacctcg gctttaggcg cacgtgctcc acggcctgaa 9660

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga 9720

9661 gccccgtcag gagccgggtt tcgtagtcga gtagctctcg gacgcgctgc ctgcgtgact 9720

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga 9780

9721 gccacagcag gtagtgtcaa acggtcacta tgtgtacccc tagtcgttag cgcgtatact 9780

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg 9840

9781 ttagtgcggt acatcacata actggctaag gaacgccagg cttacccggc ttgggcgagc 9840

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag 9900

9841 agaccgattc tagccggcgt cgctagcgta ggtatcggag gcgctggcca acatcttgtc 9900

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc 9960

9901 gcccgtcaag ccaaagtccg tccagaacgt tgcactgtgg gacacgtgcc gccctctacg 9960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 aataggtcag gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg 10020

9961 ttatccagtc cgagagcgat ttgaggggtt acagttcgtg aaggccttag ccctcgcgcc 10020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta 10080

10021 ggctacgttt cacggctatt tgtattgcta gaaacatctt tggtagccgc gtcgataaat 10080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct 10140

10081 gggcgtcctg tataggtgcg ggaggatgta gcttcgactt tcgtgctcta agaagcggga 10140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag 10200

10141 ggctctcgac gtagtccagc ctctgcgaca gcttgaaaag ctagtctttg aagagctgtc 10200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... ... XmaI(10242),SmaI(10244) ...

10201 acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct gcgaaagctc 10260

10201 tgcagcgcca ctcaagtccg aaaaagtata gagtaacggg gggccctaga cgctttcgag 10260

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat 10320

10261 ctctctctat ctaaacatct ctctctgacc actaaagtcg cacaggagag gtttacttta 10320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta 10380

10321 cttgaaggaa tatatctcct tccagaacgc ttcctatcac cctaacacgc agtagggaat 10380

.......... .......... .......... .......... .......... ..........

......... EcoRV(10394) ......... .......... .......... ..........

10381 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 10440

10381 gcagtcacct ctatagtgta gttaggtgaa cgaaacttct gcaccaacct tgcagaagaa 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 10500

10441 aaaggtgcta cgaggagcac ccacccccag gtagaaaccc tggtgacagc cgtctccgta 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt 10560

10501 gaacttgcta tcggaaagga aatagcgtta ctaccgtaaa catccacggt ggaaggaaaa 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg 10620

10561 gatgacagga aaactacttc actgtctatc gacccgttac cttaggctcc tccaaagggc 10620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga 10680

10621 tataatggga aacaactttt cagagttatc gggaaaccag aagactctga catagaaact 10680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct 10740

10681 ataagaacct catctgctct cacagcacga ggtggtacaa tagtgtagtt aggtgaacga 10740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 10800

10741 aacttctgca ccaaccttgc agaagaaaaa ggtgctacga ggagcaccca cccccaggta 10800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 10860

10801 gaaaccctgg tgacagccgt ctccgtagaa cttgctatcg gaaaggaaat agcgttacta 10860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 10920

10861 ccgtaaacat ccacggtgga aggaaaagat gacaggaaaa ctacttcact gtctatcgac 10920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 10980

10921 ccgttacctt aggctcctcc aaagggctat aatgggaaac aacttttcag agttatcggg 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 11040

10981 aaaccagaag actctgacat agaaactata agaacctcat ctgctctcac agcacgaggt 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat 11100

11041 ggtacaaccg ttcgacgaga tcggttatgc gtttggcgga gaggggcgcg caaccggcta 11100

.......... .......... .......... .......... .......... ..........

......... PvuII(11113) ......... .......... .......... ..........

11101 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 11160

11101 agtaattacg tcgaccgtgc tgtccaaagg gctgaccttt cgcccgtcac tcgcgttgcg 11160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11161 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 11220

11161 ttaattacac tcaatcgagt gagtaatccg tggggtccga aatgtgaaat acgaaggccg 11220

.......... .......... .......... ......... lac_promoter .........

.......... .......... .......... .......... .......... ..........

11221 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 11280

11221 agcatacaac acaccttaac actcgcctat tgttaaagtg tgtcctttgt cgatactggt 11280

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

.......... .......... .......... .......... .......... ..........

11281 tgattacg.. .......... .......... .......... .......... .......... 11288

11281 actaatgc.. .......... .......... .......... .......... .......... 11288

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-11288 Reference author: Shi X.G., Zhuang W.J.; Reference title: "Binary vector pCAMBIA1300-VE2-7S for making homogentisate Reference title: phytyltransferase over expression in seed"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-11288 Reference author: Zhuang W.J., Shi X.G.; Reference title: ; Reference location: Submitted (06-OCT-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Crop Science Department, Oil Crop Institution, Fujian Agriculture, Jinshan, Reference location: Fuzhou, Fujian 350002, P.R. China