Sequence of pCAMBIA1300-VE2-VE3

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Sequence of pCAMBIA1300-VE2-VE3

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Sequence of pCAMBIA1300-VE2-VE3: 13725 BP; 3560 A; 3386 C; 3487 G; 3292 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60

1 ttaagggcta gatcattgta tctactgtgg cgcgcgctat taaataggat caaacgcgcg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120

61 atataaaaca aaagatagcg cataatttac atattaacgc cctgagatta gtatttttgg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180

121 gtagagtatt tattgcagta cgtaatgtac aattaataat gtacgaattg cattaagttg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240

181 tctttaatat actattagta gcgttctggc cgttgtccta agttagaatt ctttgaaata 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgccaaatgt ttgaacgatc ggggaaattc gagctctcag tcacttcaaa aaaggtaaca 300

241 acggtttaca aacttgctag cccctttaag ctcgagagtc agtgaagttt tttccattgt 300

.......... .......... .......... .......... HPT1 ..........

ScaI(307) .......... .......... .......... .......... ..........

301 gcaagtactc tgcataaaag agcttccata tgaacatata acatgaagtt atttcggttt 360

301 cgttcatgag acgtattttc tcgaaggtat acttgtatat tgtacttcaa taaagccaaa 360

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgctactcag atcaacggac ttagctcgag cccacaaagt tgttgcgagt ataacatgac 420

361 acgatgagtc tagttgcctg aatcgagctc gggtgtttca acaacgctca tattgtactg 420

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccacaaccga gatgactttg ctccatatga atggagatgt ggctccaact agaattgcaa 480

421 ggtgttggct ctactgaaac gaggtatact tacctctaca ccgaggttga tcttaacgtt 480

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cagcgtaagc catttgaagt agtgtaacac atgtccaaaa cacccgtttc tgacccagag 540

481 gtcgcattcg gtaaacttca tcacattgtg tacaggtttt gtgggcaaag actgggtctc 540

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... EcoRV(584) ..........

541 ttacagagaa tgatcggatt ccgaatatct tatccccttc gatatcaggt atatccttaa 600

541 aatgtctctt actagcctaa ggcttataga ataggggaag ctatagtcca tataggaatt 600

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acaatgcaat aacgacagag aaaaagctca taaacgcagt ggcgaaaata agaggcctag 660

601 tgttacgtta ttgctgtctc tttttcgagt atttgcgtca ccgcttttat tctccggatc 660

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgaacaagat tggtcttcca aacacatgtg tctgaatatg tagataaaag gcgatttgaa 720

661 acttgttcta accagaaggt ttgtgtacac agacttatac atctattttc cgctaaactt 720

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 caataatagc tcggacagcg aggatacaca ttgctgcaac caatgcaaat cttttccacc 780

721 gttattatcg agcctgtcgc tcctatgtgt aacgacgttg gttacgttta gaaaaggtgg 780

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtaaaagtgg caaattgata gagtatgcag taccgagcat gaaactcaca aaaagagccc 840

781 cattttcacc gtttaactat ctcatacgtc atggctcgta ctttgagtgt ttttctcggg 840

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agaacaatgg ccatgaacca acaatccacc caagccagaa actcatgatg gagaaggaag 900

841 tcttgttacc ggtacttggt tgttaggtgg gttcggtctt tgagtactac ctcttccttc 900

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctactattgc aatgccggtg ttaacagaat attctcctga tgccaatgga aaatagggct 960

901 gatgataacg ttacggccac aattgtctta taagaggact acggttacct tttatcccga 960

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgttaacctt atctatttca acatcaaaca actgatttag cccaactatg taaatgttca 1020

961 acaattggaa tagataaagt tgtagtttgt tgactaaatc gggttgatac atttacaagt 1020

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcatgagagc tgcaacaaca gcctccaaga tgccagtgaa aagtaaagga aatatatcaa 1080

1021 agtactctcg acgttgttgt cggaggttct acggtcactt ttcatttcct ttatatagtt 1080

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaaccttctc tactgctaaa aaagatacag ataaaatgct aagcactgtg ccaataactg 1140

1081 tttggaagag atgacgattt tttctatgtc tattttacga ttcgtgacac ggttattgac 1140

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tatgaggcct aaaaaacctg taaaacgcat ctaacgagtc tctaaaaaac ttctgtttgc 1200

1141 atactccgga ttttttggac attttgcgta gattgctcag agattttttg aagacaaacg 1200

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tattcgagtc aaaagcctca ggctgacccg cagtggcatt aacccgaaat ctccacttat 1260

1201 ataagctcag ttttcggagt ccgactgggc gtcaccgtaa ttgggcttta gaggtgaata 1260

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttttggata caacaacaat gaaaatcctt gaccatcagg cctaacaaga ttgttcctaa 1320

1261 caaaacctat gttgttgtta cttttaggaa ctggtagtcc ggattgttct aacaaggatt 1320

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acttcggttt tgcgacaact ttactcgaat cacaacgcaa aactcggatt tctgataaag 1380

1321 tgaagccaaa acgctgttga aatgagctta gtgttgcgtt ttgagcctaa agactatttc 1380

.......... .......... HPT1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agtggagctt tagattctgc ttcttccaac aaaacccacc agcagcggaa acaagaaaaa 1440

1381 tcacctcgaa atctaagacg aagaaggttg ttttgggtgg tcgtcgcctt tgttcttttt 1440

.......... .......... HPT1 .......... .......... ..........

.......... NcoI(1460) .......... .......... .......... ..........

1441 aactagagag cagaaactcc atggtgaccc gggagtatct taaattattt attaaggtat 1500

1441 ttgatctctc gtctttgagg taccactggg ccctcataga atttaataaa taattccata 1500

.......... HPT1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 actaggatat tgaactagtt ctcgatgatg aaaacctgga ccgagattgc gggtatttat 1560

1501 tgatcctata acttgatcaa gagctactac ttttggacct ggctctaacg cccataaata 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agtcatagaa gagatatagc tctgttaact tgcatgcatg gggtggcatg caagttgacg 1620

1561 tcagtatctt ctctatatcg agacaattga acgtacgtac cccaccgtac gttcaactgc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggtgttgaat tgaacaaaat tgatgtgacg gaagatggtt gtgatcgagt tagagtcgca 1680

1621 ccacaactta acttgtttta actacactgc cttctaccaa cactagctca atctcagcgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 taggcagtgt acttagaaat taacaagaaa tgtgttgagc gatggtgagc aatttgggct 1740

1681 atccgtcaca tgaatcttta attgttcttt acacaactcg ctaccactcg ttaaacccga 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcatgttgag tacgtgttga tgtgcatggc atttagacct acatggacgc ggcgatcttg 1800

1741 cgtacaactc atgcacaact acacgtaccg taaatctgga tgtacctgcg ccgctagaac 1800

.......... .......... .......... .......... .......... ..........

NcoI(1807) .......... .......... .......... .......... ..........

1801 atcagccatg gatagtgaag tggggtgagg ttgcattttg tctcaatttt atttgatgga 1860

1801 tagtcggtac ctatcacttc accccactcc aacgtaaaac agagttaaaa taaactacct 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cttggacatt gctttcgaaa ggatacttta tgtgttgtct tttggttgcg catgcatgat 1920

1861 gaacctgtaa cgaaagcttt cctatgaaat acacaacaga aaaccaacgc gtacgtacta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccaagagact agatagtgtg atatgctgat atggttgctt tccaaagaga acaccgcgtt 1980

1921 ggttctctga tctatcacac tatacgacta taccaacgaa aggtttctct tgtggcgcaa 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgagatttgg ttacaaacaa caaatgtgtg ggctcatcat ggattgagtt ggtgaatgtt 2040

1981 actctaaacc aatgtttgtt gtttacacac ccgagtagta cctaactcaa ccacttacaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttgggcagc acgattgcac cttggtgagt acgtgttgtt gtgcatggct tttgggtcca 2100

2041 aaacccgtcg tgctaacgtg gaaccactca tgcacaacaa cacgtaccga aaacccaggt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctgtgtgtga ttgcgttttt gtttgtattt tatgaactta gacatttttt tgcaaaggat 2160

2101 gacacacact aacgcaaaaa caaacataaa atacttgaat ctgtaaaaaa acgtttccta 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actctttatg ttgtcttttt tgttctttgg ctttttctca tgatccaaga gactagtcag 2220

2161 tgagaaatac aacagaaaaa acaagaaacc gaaaaagagt actaggttct ctgatcagtc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgttgtggtg gcattcgaga gtaccaagat taaaagaacc gacatgcata taattacgat 2280

2221 acaacaccac cgtaagctct catggttcta attttcttgg ctgtacgtat attaatgcta 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggaagaagca taagtaattg atcggtacgg ctgttatcaa attaattaat gagcggcaaa 2340

2281 ccttcttcgt attcattaac tagccatgcc gacaatagtt taattaatta ctcgccgttt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tggaggacca ggatcaattc ccgatctagt aacatagatg acaccgcgcg cgataattta 2400

2341 acctcctggt cctagttaag ggctagatca ttgtatctac tgtggcgcgc gctattaaat 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact 2460

2401 aggatcaaac gcgcgatata aaacaaaaga tagcgcataa tttacatatt aacgccctga 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc 2520

2461 gattagtatt tttgggtaga gtatttattg cagtacgtaa tgtacaatta ataatgtacg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat 2580

2521 aattgcatta agttgtcttt aatatactat tagtagcgtt ctggccgttg tcctaagtta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcgagct cttcagatgg 2640

2581 gaattctttg aaataacggt ttacaaactt gctagcccct ttaagctcga gaagtctacc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gttggtcttt gggaacgatc tgatccttga tccacatgta gatagggatt aacacaaacc 2700

2641 caaccagaaa cccttgctag actaggaact aggtgtacat ctatccctaa ttgtgtttgg 2700

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aggcagctgc tagagttccc aggaggaagc gtcccaagaa ggagaagggg ttgttgacag 2760

2701 tccgtcgacg atctcaaggg tcctccttcg cagggttctt cctcttcccc aacaactgtc 2760

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcttctctac gtcctcttcc tttggaccaa gctggagagg agagtcaccg gaggcaggtt 2820

2761 cgaagagatg caggagaagg aaacctggtt cgacctctcc tctcagtggc ctccgtccaa 2820

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 taacaccagt gacagaacat cccatgataa ggccgtgcct gcgaacacca cggtaccact 2880

2821 attgtggtca ctgtcttgta gggtactatt ccggcacgga cgcttgtggt gccatggtga 2880

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tggggccaat cctcttgagc tgaacgtcct tgaaaccggc attcttgaac cactcaatgt 2940

2881 accccggtta ggagaactcg acttgcagga actttggccg taagaacttg gtgagttaca 2940

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 actcttcctc cttggggaag agcatccaga catcagaaaa gaagcgagag agccagaagg 3000

2941 tgagaaggag gaaccccttc tcgtaggtct gtagtctttt cttcgctctc tcggtcttcc 3000

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttgggtagac agggccgatg agacacgctt tgccaccgat cttgagaacc ctgtacgctt 3060

3001 aacccatctg tcccggctac tctgtgcgaa acggtggcta gaactcttgg gacatgcgaa 3060

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... ScaI(3091) .......... ..........

3061 cccttattcc cctctgcggg tccggccagt actcaatgct tccagcagaa acgtatctgt 3120

3061 gggaataagg ggagacgccc aggccggtca tgagttacga aggtcgtctt tgcatagaca 3120

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagcataatc ggttggaaaa ggaagatcct cagcatctcc ctcgacgatc ttgcattctt 3180

3121 gtcgtattag ccaacctttt ccttctagga gtcgtagagg gagctgctag aacgtaagaa 3180

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcaacggctc cttttgcttt gctttggcca gctgatgtgg cgactggtcc agaatggtca 3240

3181 agttgccgag gaaaacgaaa cgaaaccggt cgactacacc gctgaccagg tcttaccagt 3240

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cattcttggc cttcactgtc ttgactatgc ccagagtagt gaaaccagtt ccgccgccga 3300

3241 gtaagaaccg gaagtgacag aactgatacg ggtctcatca ctttggtcaa ggcggcggct 3300

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 catcgaccac tcgcatgtcc ggatggctga gatccgctgg ctcaagagcg tcgtctctca 3360

3301 gtagctggtg agcgtacagg cctaccgact ctaggcgacc gagttctcgc agcagagagt 3360

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tatcctcggt ccaatgccca ggattgatga catggtcgta tacgatggat aagaacctgt 3420

3361 ataggagcca ggttacgggt cctaactact gtaccagcat atgctaccta ttcttggaca 3420

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agaaccagta agcctccttc ttgtgctgaa tgaacctagg ttgcgccgat ggccgggaag 3480

3421 tcttggtcat tcggaggaag aacacgactt acttggatcc aacgcggcta ccggcccttc 3480

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 acgacacgct gctgctgctg catctagtag ccaccgagag tctaggtgtg gaggttcggg 3540

3481 tgctgtgcga cgacgacgac gtagatcatc ggtggctctc agatccacac ctccaagccc 3540

.......... .......... APG1 .......... .......... ..........

.......... EagI(3558) .......... .......... .......... ..........

3541 taactgagag taaggtcggc cgaggaatcg atctggcatg caaattggaa ccaggggaac 3600

3541 attgactctc attccagccg gctccttagc tagaccgtac gtttaacctt ggtccccttg 3600

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .... NcoI(3647) XmaI(3656),SmaI(3658)

3601 ctaaaccttt ggggaaggta atggccccgt tgagcatcaa agaggccatg gtgacccggg 3660

3601 gatttggaaa ccccttccat taccggggca actcgtagtt tctccggtac cactgggccc 3660

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcgtgtatgt ttttaatctt gtttgtattg atgagttttg gtttgagtaa agagtgaagc 3720

3661 agcacataca aaaattagaa caaacataac tactcaaaac caaactcatt tctcacttcg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggatgagtta atttataggc tataaaggag atttgcatgg cgatcacgtg taataatgca 3780

3721 cctactcaat taaatatccg atatttcctc taaacgtacc gctagtgcac attattacgt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgcacgcatg tgattgtatg tgtgtgctgt gagagagaag ctcttaggtg tttgaaggga 3840

3781 acgtgcgtac actaacatac acacacgaca ctctctcttc gagaatccac aaacttccct 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtgacaagtg gcgaagaaaa acaattctcc gcggctgcat gctatgtgta acgtgtagct 3900

3841 cactgttcac cgcttctttt tgttaagagg cgccgacgta cgatacacat tgcacatcga 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aatgttctgg catggcatct tatgaacgat tctttttaaa aacaaggtaa aaacttaact 3960

3901 ttacaagacc gtaccgtaga atacttgcta agaaaaattt ttgttccatt tttgaattga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcataaaatt aaaaaaaaaa aacgtttact aagttggttt aaaaggggat gagagtctat 4020

3961 agtattttaa tttttttttt ttgcaaatga ttcaaccaaa ttttccccta ctctcagata 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaattttgga ggtagtgccg ttgggaatat aaattgggag cttaatcaga attatagaag 4080

4021 tttaaaacct ccatcacggc aacccttata tttaaccctc gaattagtct taatatcttc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttaaagttga tttagtcacg gtcaatataa attgggaatt tgagtcaaaa tcttccaaat 4140

4081 aatttcaact aaatcagtgc cagttatatt taacccttaa actcagtttt agaaggttta 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcggaatccg tcttgttaca cccggtggat aggagccgaa cggtttgaaa atacttgaaa 4200

4141 agccttaggc agaacaatgt gggccaccta tcctcggctt gccaaacttt tatgaacttt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgtggatgca ggtgcaggct ggtttaattt tatgttgaat ggatacatgt caatcgaatt 4260

4201 acacctacgt ccacgtccga ccaaattaaa atacaactta cctatgtaca gttagcttaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgagttatag gtacacattt tactctgata ctaaaatgta acatttgtct caagaatggg 4320

4261 actcaatatc catgtgtaaa atgagactat gattttacat tgtaaacaga gttcttaccc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 taggtcatcc ttactcgaga acggtatgcc attcacttga acgtatatct cattcaaccc 4380

4321 atccagtagg aatgagctct tgccatacgg taagtgaact tgcatataga gtaagttggg 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aactctaaca cacatttcat acccatgtct aaaataaaga aaattgaaga aaaacgctca 4440

4381 ttgagattgt gtgtaaagta tgggtacaga ttttatttct tttaacttct ttttgcgagt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atagcgtcaa tccttacaaa tataatgtac atggcaacct cgcatgaaaa tagtaataaa 4500

4441 tatcgcagtt aggaatgttt atattacatg taccgttgga gcgtactttt atcattattt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gaggagtgaa taatataaag tagtcactaa ttggatgaca aagcaaatgg aaccatccga 4560

4501 ctcctcactt attatatttc atcagtgatt aacctactgt ttcgtttacc ttggtaggct 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tagatgtttt gagaacaaga ggtacatttg tatatatgca ttggttgata catttctgcc 4620

4561 atctacaaaa ctcttgttct ccatgtaaac atatatacgt aaccaactat gtaaagacgg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 atctgaattg tttcttcctc aaagaagaag cattcagcca atagtgaaat ttaaaatgtt 4680

4621 tagacttaac aaagaaggag tttcttcttc gtaagtcggt tatcacttta aattttacaa 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggaaaaatga ggatgcagtc agaaagtcca gaatataagt ggtaactggt aactgaatac 4740

4681 cctttttact cctacgtcag tctttcaggt cttatattca ccattgacca ttgacttatg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttgcctcaga agcaagataa gaaacataag tctttaaagg aatcaatcac cgatgaagaa 4800

4741 aacggagtct tcgttctatt ctttgtattc agaaatttcc ttagttagtg gctacttctt 4800

.......... .......... .......... .......... .......... ..........

HindIII(4801) PstI(4811) ...... .......... .......... ..........

4801 agcttctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa 4860

4801 tcgaagacgt ccgtacgttc gaaccgtgac cggcagcaaa atgttgcagc actgaccctt 4860

.......... .......... . M13_forward20_primer M13_pUC_fwd_primer .

.......... .......... .......... .......... .......... ..........

4861 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 4920

4861 ttgggaccgc aatgggttga attagcggaa cgtcgtgtag ggggaaagcg gtcgaccgca 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 4980

4921 ttatcgcttc tccgggcgtg gctagcggga agggttgtca acgcgtcgga cttaccgctt 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca 5040

4981 acgatctcgt cgaactcgaa cctagtctaa cagcaaaggg cggaagtcaa atttgatagt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac 5100

5041 cacaaactgt cctatataac cgcccatttg gattctcttt tctcgcaaat aatcttattg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac 5160

5101 cctataaatt ttcccgcact tttccaaata ggcaagcagg taaacataca cgtacggttg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... ScaI(5187) .......... .......... ..........

5161 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag 5220

5161 gtgtcccaag gggagcccta gtttcatgaa actaggttgg ggaggcgacg atatcacgtc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag 5280

5221 agccgaagac tgcaagtcac gtcggcagaa gacttttgct gtacagcgtg ttcaggattc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc 5340

5281 aatgcgctgt ccgacggcgg gacgggaaaa ggaccgcaaa agaacagcgc acaaaatcag 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg 5400

5341 cgtatttcat cttatgaacg ctgatcttgg cctctgtaat gcggtacttg ttctcgcggc 5400

.......... .......... .......... .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

5401 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac 5460

5401 ggcgaccgga cgacccgata cgggcgcagt cgtggctgct ggtcctgaac tggttggttg 5460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca 5520

5461 cccggcttga cgtgcgccgg ccgacgtggt tcgacaaaag gctcttctag tggccgtggt 5520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga 5580

5521 ccgcgctggc gggcctcgac cggtcctacg aactggtgga tgcgggaccg ctgcaacact 5580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc 5640

5581 gtcactggtc cgatctggcg gaccgggcgt cgtgggcgct ggatgacctg taacggctcg 5640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca 5700

5641 cgtaggtcct ccggccgcgc ccggacgcat cggaccgtct cggcacccgg ctgtggtggt 5700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc 5760

5701 gcggccggcc ggcgtaccac aactggcaca agcggccgta acggctcaag ctcgcaaggg 5760

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg 5820

5761 attagtagct ggcgtgggcc tcgcccgcgc tccggcggtt ccgggctccg cacttcaaac 5820

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg 5880

5821 cgggggcggg atgggagtgg ggccgtgtct agcgcgtgcg ggcgctcgac tagctggtcc 5880

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc 5940

5881 ttccggcgtg gcactttctc cgccgacgtg acgaaccgca cgtagcgagc tgggacatgg 5940

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc 6000

5941 cgcgtgaact cgcgtcgctc cttcactgcg ggtggctccg gtccgccgcg ccacggaagg 6000

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg 6060

6001 cactcctgcg taactggctc cggctgcggg accgccggcg gctcttactt gcggttctcc 6060

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat 6120

6061 ttgttcgtac tttggcgtgg tcctgccggt cctgcttggc aaaaagtaat ggcttctcta 6120

ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt 6180

6121 gctccgcctc tactagcgcc ggcccatgca caagctcggc gggcgcgtgc agagttggca 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag 6240

6181 cgccgacgta ctttaggacc ggccaaacag actacggttc gaccgccgga ccggccggtc 6240

.......... .......... .......... .......... .......... ..........

SfiI(6243) .......... .......... .......... .......... ..........

6241 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 6300

6241 gaaccggcga cttctttggc tcgcggcggc agatttttcc actacacata aactcatttt 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 6360

6301 gtcgaacgca gtacgccagc gacgcatata ctacgctact catttatttg tttatgcgtt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 6420

6361 ccccttgcgt acttccaata gcgacatgaa ttggtctttc cgcccagtcc gttctgctgg 6420

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 6480

6421 tagcgttggg tagatcgggc gcgggacgtt gagcggcccc ggctacaaga caatcagcta 6480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 6540

6481 aggctagggg tcccgtcacg ggcgctaacc cgccggcacg cccttctagt tggcgattgg 6540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 6600

6541 caacagccgt agctggcggg ctgctaactg gcgctgcact tccggtagcc ggccgcgctg 6600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 6660

6601 aagcatcact agctgcctcg cggggtccgc cgcctgaacc gacacaggcg ctagttccgt 6660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... NdeI(6704) ..........

6661 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 6720

6661 cggctgaagc acgactaagg ccacgtcggt tcgggaatgc tgtatacccg gtggcggctg 6720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 6780

6721 gaccacctcg accaattcgt cgcgtaactc cagtgcctac cttccgatgt tcgccggaaa 6780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 6840

6781 cagcacagcg cccgctagtt tccgtgcgcg tagccgccac tccaacggct ccgcgaccgg 6840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 6900

6841 cccatgctcg acgggtaaga actcagggca tagtgcgtcg cgcactcgat gggtccgtga 6900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 6960

6901 cggcggcggc cgtgttggca agaacttagt cttgggctcc cgctgcgacg ggcgctccag 6960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 7020

6961 gtccgcgacc ggcgacttta atttagtttt gagtaaactc aattactcca tttctctttt 7020

.......... ORF 1 .......... .......... .......... ..........

.......... .......... EagI(7050) .......... .......... ..........

7021 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 7080

7021 actcgttttc gtgtttgtgc gattcacggc cggcaggctc gcgtgcgtcg tcgttccgac 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 7140

7081 gttgcaaccg gtcggaccgt ctgtgcggtc ggtacttcgc ccagttgaaa gtcaacggcc 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 7200

7141 gcctcctagt gtggttcgac ttctacatgc gccatgcggt tccgttctgg taatggctcg 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 7260

7201 acgatagact tatgtagcgc gtcgatggtc tcatttactc gtttacttat ttactcatct 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 7320

7261 acttaaaatc gccgatttcc tccgccgtac cttttagttc ttgttggtcc gtggctgcgg 7320

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 7380

7321 caccttacgg ggtacacacc tccttgcccg ccaaccggtc cgcattcgcc gacccaacag 7380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa 7440

7381 acggccggga cgttaccgtg accttggggg ttcgggctcc ttagccgcac tgccagcgtt 7440

.......... .......... ORF 3 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

7441 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa 7500

7441 tggtaggccg ggccatgttt agccgcgccg cgacccacta ctggaccacc tcttcaactt 7500

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg 7560

7501 ccggcgcgtc cggcgggtcg ccgttgcgta gctccgtctt cgtgcggggc cacttagcac 7560

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc 7620

7561 cgttcgccgg cgactagctt aggcgtttct tagggccgtt ggcggccgtc ggccacgcgg 7620

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta 7680

7621 cagctaatcc ttcggcgggt tcccgctgct cgttggtcta aaaaagcaag gctacgagat 7680

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa 7740

7681 actgcacccg tgggcgctat cagcgtcgta gtacctgcac cggcaaaagg cagacagctt 7740

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt 7800

7741 cgcactggct gctcgaccgc tccactaggc gatgctcgaa ggtctgcccg tgcatctcca 7800

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... EagI(7813) SfiI(7821) .......... .......... ..........

7801 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt 7860

7801 aaggcgtccc ggccggccgt accggtcaca caccctaatg ctggaccatg actaccgcca 7860

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg 7920

7861 aagggtagat tggcttaggt acttggctat ggcccttccc ttccctctgt tcgggccggc 7920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa 7980

7921 gcacaaggca ggtgtgcaac gcctgcatga gttcaagacg gccgctcggc taccgccttt 7980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca 8040

7981 cgtctttctg ctggaccatc tttggacgta agccaatttg tggtgcgtgc aacggtacgt 8040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat 8100

8041 cgcatgcttc ttccggttct tgccggcgga ccactgccat aggctcccac ttcggaacta 8100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... EagI(8136) .......... ..........

8101 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct 8160

8101 atcggcgatg ttctagcatt tctcgctttg gcccgccggc ctcatgtagc tctagctcga 8160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca 8220

8161 tcgactaacc tacatggcgc tctagtgtct tccgttcttg ggcctgcacg actgccaagt 8220

.......... .......... ORF 2 .......... .......... ..........

.......... ClaI(8239) .......... EagI(8252) .......... ..........

8221 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg 8280

8221 ggggctaatg aaaaactagc tagggccgta gccggcaaaa gagatggcgg accgtgcggc 8280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag 8340

8281 gcggcgtccg ttccgtcttc ggtctaccaa caagttctgc tagatgcttg cgtcaccgtc 8340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct 8400

8341 gcggcctctc aagttcttca agacaaagtg gcacgcgttc gactagccca gtttactgga 8400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta 8460

8401 cggcctcatg ctaaacttcc tcctccgccc cgtccgaccg ggctaggatc agtacgcgat 8460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg 8520

8461 ggcgttggac tagctcccgc ttcgtaggcg gccaaggatt acatgcctcg tctacgatcc 8520

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta 8580

8521 cgtttaacgg gatcgtcccc tttttccagc ttttccagag aaaggacacc tatcgtgcat 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc 8640

8581 gtaacccttg ggtttcggca tgtaaccctt ggccttgggc atgtaaccct tgggtttcgg 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt 8700

8641 catgtaaccc ttggccagtg tgtacattca ctgactatat tttctctttt ttccgctaaa 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata 8760

8701 aaggcggatt ttgagaaatt ttgaataatt ttgagaattt tgggcggacc ggacacgtat 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg 8820

8761 tgacagaccg gtcgcgtgtc ggcttctcga cgtttttcgc ggatgggaag ccagcgacgc 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc 8880

8821 gagggatgcg gggcggcgaa gcgcagccgg atagcgccgg cgaccggcga gtttttaccg 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg 8940

8881 accggatgcc ggtccgttag atggtcccgc gcctgttcgg cgcggcagcg gtgagctggc 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 9000

8941 ggccgcgggt gtagttccgt gggacggagc gcgcaaagcc actactgcca cttttggaga 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 9060

9001 ctgtgtacgt cgagggcctc tgccagtgtc gaacagacat tcgcctacgg ccctcgtctg 9060

pGEX_3_primer ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 9120

9061 ttcgggcagt cccgcgcagt cgcccacaac cgcccacagc cccgcgtcgg tactgggtca 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 9180

9121 gtgcatcgct atcgcctcac atatgaccga attgatacgc cgtagtctcg tctaacatga 9180

.......... .......... .......... .......... .......... ..........

.......... NdeI(9193) .......... .......... .......... ..........

9181 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9240

9181 ctctcacgtg gtatacgcca cactttatgg cgtgtctacg cattcctctt ttatggcgta 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 9300

9241 gtccgcgaga aggcgaagga gcgagtgact gagcgacgcg agccagcaag ccgacgccgc 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 9360

9301 tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc ccctattgcg 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 9420

9361 tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 9480

9421 cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag ctgcgagttc 9480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 9540

9481 agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg gaccttcgag 9540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 9600

9541 ggagcacgcg agaggacaag gctgggacgg cgaatggcct atggacaggc ggaaagaggg 9600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 9660

9601 aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc atagagtcaa gccacatcca 9660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 9720

9661 gcaagcgagg ttcgacccga cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa 9720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 9780

9721 taggccattg atagcagaac tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg 9780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 9840

9781 tcggtgacca ttgtcctaat cgtctcgctc catacatccg ccacgatgtc tcaagaactt 9840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 9900

9841 caccaccgga ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc gagacgactt 9900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 9960

9901 cggtcaatgg aagccttttt ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc 9960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 10020

9961 atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct 10020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 10080

10021 tctaggaaac tagaaaagat gccccagact gcgagtcacc ttgcttttga gtgcaattcc 10080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat 10140

10081 ctaaaaccag tacgtaagat ccatgatttt gttaagtagg tcattttata ttataaaata 10140

.......... .......... .......... ......... KanR1 ORF 1 .........

.......... .......... .......... .......... .......... ..........

10141 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc 10200
tgacaagaag
10141 aaagagggtt agtccgaact aggggtcatt cagtttttta tcgagctgta 10200

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct 10260
acaggcggga
10201 gggctatagg agggactagc tggcctgcgt cttccgttac agtatggtga 10260

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct 10320
tctacaacga
10261 cggcgaagag ggttctagtt atttcggtga atgaaacggt agaaagtgtt 10320

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa 10380
agaaattttt
10321 cagagggtcc agcggcaccc ttttctgttc aaggagaagc ccgaaaaggc 10380

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac 10440
gcgttaggtg
10381 tagtatgtcg agcgcgccta gaaatttacc tcacagaaga agggtcaaaa 10440

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt 10500
gattcgataa
10441 tagccggtct agcaataagt cattcattag gttaagccga ttcgccgaca 10500

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag 10560
ggcgtatgtc
10501 gcatatccct gttaggctat acagctacct cactttctcg gactacgtga 10560

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc 10620
cctgcggtag
10561 gagctattag aaaagtcccg aaacaagtag aagtatgaga aggctcgttt 10620

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg 10680
cctggaaacc
10621 ccggagtgag tactcgtcta acgaggtcgg tagtacggca agtttcacgt 10680

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt 10740
gtagtatcca
10681 ttgtccgtcg aaaggaaggt cggtatcgta gtacaggaaa agggcaaggt 10740

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag 10800
gagggtggtc
10741 ccagggaaat atggccgaca ggcagtaaaa atttatatcc aaaagtaaaa 10800

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat 10860
taaaaagcta
10801 gaatatatgg aatcgtcctc tgtaaggaag gcatagaaaa tgcgtcgcca 10860

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt 10920

10861 gtcaaaaaag ttaaggccac tataagagta aaatcggtaa ataataaagg aaggagaaaa 10920

......... KanR1 ORF 1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg 10980

10921 gatgtcataa atttctatgg ggttcttcga ttaatattgt tctgcttgag gttaagtgac 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag 11040

10981 aaggaacgta agattttgga atttatggtc ttttgtcgaa aaagtttcaa caaaagtttc 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BclI(11087) .........

11041 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca 11100

11041 aaccgcatat tgtatcatag ctgcctcggc taaaactttg gcgccactag tgtccgtcgt 11100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11101 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac 11160

11101 tgcgagacag tagcaatgtt agttgtacga tgggaggcgc tctagtaggc acaaagtttg 11160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11161 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc 11220

11161 ggccgtcgaa tcaacggcaa gaaggcttat cgtagccatt gtactcgttt cagacggcgg 11220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11221 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg 11280

11221 aatgttgccg agagggcgac tgcggcaggg cctgactacc cgacggacat agctcaccac 11280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg 11340

11281 taaaacacgg ctcgacggcc agcccctcga caaccgaccg accaccgtcc tatataacac 11340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11341 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact 11400

11341 cacatttgtt taactgcgaa tctgttgaat tattgtgtaa cgcctgcaaa aattacatga 11400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11401 gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga 11460

11401 cttaattgcg gcttaattaa gccccctaga cctaaaatca tgacctaaaa ccaaaatcct 11460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11461 attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta 11520

11461 taatctttaa aataactatc ttcataaaat gtttatgttt atgtatgatt cccaaagaat 11520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11521 tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact 11580

11521 atacgagttg tgtactcgct ttgggatatc cttgggatta agggaataga cccttgatga 11580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11581 cacacattat tatggagaaa ctcgagcttg tcgatcgaca gatccggtcg gcatctactc 11640

11581 gtgtgtaata atacctcttt gagctcgaac agctagctgt ctaggccagc cgtagatgag 11640

.......... .......... .......... ORF 4 .......... ..........

.......... .......... .......... .......... .......... ..........

11641 tatttctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct 11700

11641 ataaagaaac gggagcctgc tcacgacccc gcagccaaag gtgatagccg ctcatgaaga 11700

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

11701 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc 11760

11701 tgtgtcggta gccaggtctg ccggcgcgaa gacgcccgct aaacacatgc gggctgtcag 11760

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

11761 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa 11820

11761 ggccgaggcc tagcctgcta acgcagcgta gctgggacgc gggttcgacg tagtagcttt 11820

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

11821 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg 11880

11821 aacggcagtt ggttcgagac tatctcaacc agttctggtt acgcctcgta tatgcgggcc 11880

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

11881 agtcgtggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc 11940

11881 tcagcaccgc taggacgttc gaggcctacg gaggcgagct tcatcgcgca gacgacgagg 11940

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

11941 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg 12000

11941 tatgttcggt tggtgccgga ggtcttcttc tacaaccgct ggagcataac ccttaggggc 12000

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

12001 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg 12060

12001 ttgtagcgga gcgaggtcag ttactggcga caatacgccg gtaacaggca gtcctgtaac 12060

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

12061 ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc 12120

12061 aacctcggct ttaggcgcac gtgctccacg gcctgaagcc ccgtcaggag ccgggtttcg 12120

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

12121 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc 12180

12121 tagtcgagta gctctcggac gcgctgcctg cgtgactgcc acagcaggta gtgtcaaacg 12180

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

12181 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga 12240

12181 gtcactatgt gtacccctag tcgttagcgc gtatacttta gtgcggtaca tcacataact 12240

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... ......... EagI(12291)

12241 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg 12300

12241 ggctaaggaa cgccaggctt acccggcttg ggcgagcaga ccgattctag ccggcgtcgc 12300

.......... ......... ORF 4 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

12301 atcgcatcca tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg 12360

12301 tagcgtaggt atcggaggcg ctggccaaca tcttgtcgcc cgtcaagcca aagtccgtcc 12360

.......... ORF 4 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12361 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac 12420

12361 agaacgttgc actgtgggac acgtgccgcc ctctacgtta tccagtccga gagcgatttg 12420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12421 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca 12480

12421 aggggttaca gttcgtgaag gccttagccc tcgcgccggc tacgtttcac ggctatttgt 12480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12481 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct 12540

12481 attgctagaa acatctttgg tagccgcgtc gataaatggg cgtcctgtat aggtgcggga 12540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12541 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag 12600

12541 ggatgtagct tcgactttcg tgctctaaga agcgggaggc tctcgacgta gtccagcctc 12600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12601 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt 12660

12601 tgcgacagct tgaaaagcta gtctttgaag agctgtctgc agcgccactc aagtccgaaa 12660

.......... .......... ORF 2 .......... .......... ..........

.......... ... XmaI(12679),SmaI(12681) ... .......... ..........

12661 ttcatatctc attgcccccc gggatctgcg aaagctcgag agagatagat ttgtagagag 12720

12661 aagtatagag taacgggggg ccctagacgc tttcgagctc tctctatcta aacatctctc 12720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12721 agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg 12780

12721 tctgaccact aaagtcgcac aggagaggtt tactttactt gaaggaatat atctccttcc 12780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(12831)

12781 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa 12840

12781 agaacgcttc ctatcaccct aacacgcagt agggaatgca gtcacctcta tagtgtagtt 12840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12841 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt 12900

12841 aggtgaacga aacttctgca ccaaccttgc agaagaaaaa ggtgctacga ggagcaccca 12900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12901 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta 12960

12901 cccccaggta gaaaccctgg tgacagccgt ctccgtagaa cttgctatcg gaaaggaaat 12960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12961 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga 13020

12961 agcgttacta ccgtaaacat ccacggtgga aggaaaagat gacaggaaaa ctacttcact 13020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13021 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc 13080

13021 gtctatcgac ccgttacctt aggctcctcc aaagggctat aatgggaaac aacttttcag 13080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13081 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg 13140

13081 agttatcggg aaaccagaag actctgacat agaaactata agaacctcat ctgctctcac 13140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13141 tcgtgctcca ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct 13200

13141 agcacgaggt ggtacaatag tgtagttagg tgaacgaaac ttctgcacca accttgcaga 13200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13201 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag 13260

13201 agaaaaaggt gctacgagga gcacccaccc ccaggtagaa accctggtga cagccgtctc 13260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13261 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc 13320

13261 cgtagaactt gctatcggaa aggaaatagc gttactaccg taaacatcca cggtggaagg 13320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13321 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt 13380

13321 aaaagatgac aggaaaacta cttcactgtc tatcgacccg ttaccttagg ctcctccaaa 13380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13381 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct 13440

13381 gggctataat gggaaacaac ttttcagagt tatcgggaaa ccagaagact ctgacataga 13440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13441 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc 13500

13441 aactataaga acctcatctg ctctcacagc acgaggtggt acaaccgttc gacgagatcg 13500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... PvuII(13550) .........

13501 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 13560

13501 gttatgcgtt tggcggagag gggcgcgcaa ccggctaagt aattacgtcg accgtgctgt 13560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13561 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 13620

13561 ccaaagggct gacctttcgc ccgtcactcg cgttgcgtta attacactca atcgagtgag 13620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13621 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 13680

13621 taatccgtgg ggtccgaaat gtgaaatacg aaggccgagc atacaacaca ccttaacact 13680

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

13681 gcggataaca atttcacaca ggaaacagct atgaccatga ttacg..... .......... 13725

13681 cgcctattgt taaagtgtgt cctttgtcga tactggtact aatgc..... .......... 13725

.. M13_pUC_rev_primer M13_reverse_primer .. .......... ..........

References

Reference number: [1] Reference positions: 1-13725 Reference author: Shi X.G., Zhuang W.J.; Reference title: "Binary vector pCAMBIA1300AT for making homogentisate phytyltransferase and Reference title: MPBQ/MSBQ methyltransferase over expression in seed"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-13725 Reference author: Zhuang W.J., Shi X.G.; Reference title: ; Reference location: Submitted (08-OCT-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Crop Science Department, Oil Crop Institution, Fujian Agriculture, Jinshan, Reference location: Fuzhou, Fujian 350002, P.R. China