i5i5HID FJ362599; SV 1; circular; other DNA; STD; SYN; 11369 BP. AC FJ362599; DT 16-DEC-2008 (Rel. 98, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 1) DE Binary vector pCAMBIA1300-VE3-2S, complete sequence. KW . OS Binary vector pCAMBIA1300-VE3-2S OC other sequences; artificial sequences; vectors. RN [1] RP 1-11369 RA Shi X.G., Zhuang W.J.; RT "Binary vector pCAMBIA1300-VE3-2S for making MPBQ/MSBQ methyltransferase RT over expression in seed"; RL Unpublished. RN [2] RP 1-11369 RA Zhuang W.J., Shi X.G.; RT ; RL Submitted (08-OCT-2008) to the EMBL/GenBank/DDBJ databases. RL Crop Science Department, Oil Crop Institution, Fujian Agriculture, Jinshan, RL Fuzhou, Fujian 350002, P.R. China FH Key Location/Qualifiers FT source 1..11369 FT /organism="Binary vector pCAMBIA1300-VE3-2S" FT /mol_type="other DNA" FT /db_xref="taxon:571928" FT terminator 8..260 FT /note="NOS" FT gene complement(277..1293) FT /gene="APG1" FT /note="VTE3" FT CDS complement(277..1293) FT /codon_start=1 FT /transl_table=11 FT /gene="APG1" FT /product="MPBQ" FT /note="derived from Arabidopsis thaliana" FT /protein_id="ACJ54267.1" FT /translation="MASLMLNGAITFPKGLGSPGSNLHARSIPRPTLLSVTRTSTPRLS FT VATRCSSSSVSSSRPSAQPRFIQHKKEAYWFYRFLSIVYDHVINPGHWTEDMRDDALEP FT ADLSHPDMRVVDVGGGTGFTTLGIVKTVKAKNVTILDQSPHQLAKAKQKEPLKECKIVE FT GDAEDLPFPTDYADRYVSAGSIEYWPDPQRGIREAYRVLKIGGKACLIGPVYPTFWLSR FT FFSDVWMLFPKEEEYIEWFKNAGFKDVQLKRIGPKWYRGVRRHGLIMGCSVTGVKPASG FT DSPLQLGPKEEDVEKPVNNPFSFLGRFLLGTLAAAWFVLIPIYMWIKDQIVPKDQPI" FT promoter 1305..2449 FT /note="Brassica napus 2S seed storage protein promoter" FT misc_feature 2709..2734 FT /note="right border T-DNA repeat" FT CDS complement(7749..8543) FT /codon_start=1 FT /transl_table=11 FT /product="aminoglycoside phosphotransferase" FT /protein_id="ACJ54265.1" FT /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM FT TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED FT EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT FT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FT FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" FT misc_feature 8968..8993 FT /note="left border C58 T-DNA repeat" FT misc_feature 9060..9268 FT /note="CaMV polyA signal" FT CDS complement(9284..10309) FT /codon_start=1 FT /transl_table=11 FT /product="hygromycin phosphotransferase" FT /note="hptII; confers hygromycin resistance" FT /protein_id="ACJ54266.1" FT /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG FT YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP FT ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ FT TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF FT GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG FT NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK FT K" FT promoter 10345..11125 FT /note="CaMV 35S promoter" SQ Sequence 11369 BP; 2805 A; 2947 C; 2985 G; 2632 T; 0 other; aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240 tgccaaatgt ttgaacgatc ggggaaattc gagctctcag atgggttggt ctttgggaac 300 gatctgatcc ttgatccaca tgtagatagg gattaacaca aaccaggcag ctgctagagt 360 tcccaggagg aagcgtccca agaaggagaa ggggttgttg acaggcttct ctacgtcctc 420 ttcctttgga ccaagctgga gaggagagtc accggaggca ggtttaacac cagtgacaga 480 acatcccatg ataaggccgt gcctgcgaac accacggtac cacttggggc caatcctctt 540 gagctgaacg tccttgaaac cggcattctt gaaccactca atgtactctt cctccttggg 600 gaagagcatc cagacatcag aaaagaagcg agagagccag aaggttgggt agacagggcc 660 gatgagacac gctttgccac cgatcttgag aaccctgtac gcttccctta ttcccctctg 720 cgggtccggc cagtactcaa tgcttccagc agaaacgtat ctgtcagcat aatcggttgg 780 aaaaggaaga tcctcagcat ctccctcgac gatcttgcat tctttcaacg gctccttttg 840 ctttgctttg gccagctgat gtggcgactg gtccagaatg gtcacattct tggccttcac 900 tgtcttgact atgcccagag tagtgaaacc agttccgccg ccgacatcga ccactcgcat 960 gtccggatgg ctgagatccg ctggctcaag agcgtcgtct ctcatatcct cggtccaatg 1020 cccaggattg atgacatggt cgtatacgat ggataagaac ctgtagaacc agtaagcctc 1080 cttcttgtgc tgaatgaacc taggttgcgc cgatggccgg gaagacgaca cgctgctgct 1140 gctgcatcta gtagccaccg agagtctagg tgtggaggtt cgggtaactg agagtaaggt 1200 cggccgagga atcgatctgg catgcaaatt ggaaccaggg gaacctaaac ctttggggaa 1260 ggtaatggcc ccgttgagca tcaaagaggc catggtgacc cgggtcgtgt atgtttttaa 1320 tcttgtttgt attgatgagt tttggtttga gtaaagagtg aagcggatga gttaatttat 1380 aggctataaa ggagatttgc atggcgatca cgtgtaataa tgcatgcacg catgtgattg 1440 tatgtgtgtg ctgtgagaga gaagctctta ggtgtttgaa gggagtgaca agtggcgaag 1500 aaaaacaatt ctccgcggct gcatgctatg tgtaacgtgt agctaatgtt ctggcatggc 1560 atcttatgaa cgattctttt taaaaacaag gtaaaaactt aacttcataa aattaaaaaa 1620 aaaaaacgtt tactaagttg gtttaaaagg ggatgagagt ctataaattt tggaggtagt 1680 gccgttggga atataaattg ggagcttaat cagaattata gaagttaaag ttgatttagt 1740 cacggtcaat ataaattggg aatttgagtc aaaatcttcc aaattcggaa tccgtcttgt 1800 tacacccggt ggataggagc cgaacggttt gaaaatactt gaaatgtgga tgcaggtgca 1860 ggctggttta attttatgtt gaatggatac atgtcaatcg aatttgagtt ataggtacac 1920 attttactct gatactaaaa tgtaacattt gtctcaagaa tgggtaggtc atccttactc 1980 gagaacggta tgccattcac ttgaacgtat atctcattca acccaactct aacacacatt 2040 tcatacccat gtctaaaata aagaaaattg aagaaaaacg ctcaatagcg tcaatcctta 2100 caaatataat gtacatggca acctcgcatg aaaatagtaa taaagaggag tgaataatat 2160 aaagtagtca ctaattggat gacaaagcaa atggaaccat ccgatagatg ttttgagaac 2220 aagaggtaca tttgtatata tgcattggtt gatacatttc tgccatctga attgtttctt 2280 cctcaaagaa gaagcattca gccaatagtg aaatttaaaa tgttggaaaa atgaggatgc 2340 agtcagaaag tccagaatat aagtggtaac tggtaactga atacttgcct cagaagcaag 2400 ataagaaaca taagtcttta aaggaatcaa tcaccgatga agaaagcttc tgcaggcatg 2460 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 2520 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 2580 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga 2640 gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat 2700 attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc 2760 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg 2820 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc 2880 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc 2940 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac 3000 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg 3060 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc 3120 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga 3180 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga 3240 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg 3300 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat 3360 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac 3420 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct 3480 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa 3540 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag 3600 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac 3660 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg 3720 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc 3780 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc 3840 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa 3900 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg 3960 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg 4020 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag 4080 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca 4140 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc 4200 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg 4260 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga 4320 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta 4380 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga 4440 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca 4500 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa 4560 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg 4620 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa 4680 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct 4740 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa 4800 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat 4860 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta 4920 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt 4980 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg 5040 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta 5100 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc 5160 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat 5220 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg 5280 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc 5340 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct 5400 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc 5460 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa 5520 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac 5580 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg 5640 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc 5700 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc 5760 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac 5820 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg 5880 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca 5940 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag 6000 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg 6060 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag 6120 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca 6180 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag 6240 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg 6300 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct 6360 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc 6420 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc 6480 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc 6540 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa 6600 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 6660 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 6720 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 6780 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 6840 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 6900 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6960 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7020 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7080 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7140 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7200 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7260 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7320 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7380 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7440 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7500 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7560 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7620 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7680 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat 7740 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc 7800 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg 7860 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga 7920 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg 7980 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc 8040 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta 8100 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc 8160 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca 8220 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc 8280 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct 8340 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg 8400 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca 8460 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc 8520 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga 8580 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa 8640 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag 8700 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt 8760 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc 8820 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc 8880 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg 8940 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac 9000 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt 9060 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg 9120 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga 9180 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga 9240 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg 9300 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc 9360 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga 9420 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc 9480 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg 9540 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg 9600 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc 9660 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg 9720 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag 9780 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg 9840 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc 9900 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct 9960 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac 10020 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca 10080 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga 10140 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga 10200 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt 10260 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc 10320 ccccgggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag 10380 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt 10440 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag 10500 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg 10560 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt 10620 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat 10680 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt 10740 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt 10800 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc 10860 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag 10920 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt 10980 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt 11040 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt 11100 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc 11160 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 11220 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 11280 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 11340 cacaggaaac agctatgacc atgattacg 11369 i,pCAMBIA1300-VE3-2S"Times New Roman i51EcoRI(1),SacI(276),PvuII(351),KpnI(521),ScaI(735) Arial_i,mcYY i5APvuII(856),EagI(1202),ClaI(1213),NcoI(1291),XmaI(1300),SmaI(1302) Ariali,OU[[A i5HXhoI(1979),HindIII(2445),PstI(2455),HindIII(2463),PvuII(2558),PmeI(2676) Arial Vi,: i56ScaI(2831),EagI(3121),EagI(3349),EagI(3353),SfiI(3444) Arial i,u i5cNotI(3679),EagI(3679),EagI(3783),SfiI(3878),SfiI(3887),EagI(4157),EagI(4233),NdeI(4348),SexAI(4364) Arial/i,B i57EagI(4694),SexAI(5127),SfiI(5153),NotI(5211),EagI(5211) Arial!i,* C#L#._j i5oEagI(5457),SfiI(5465),SexAI(5487),EagI(5560),SexAI(5637),EagI(5707),EagI(5780),NheI(5803),ClaI(5883),EagI(5896) Ariali,4>YY  i5 EagI(6501),NotI(6501),NdeI(6837) Arialei,`\(W1 1WU i5EcoRV Ariali, i56BclI(8731),ScaI(9085),XhoI(9246),ScaI(9339),EagI(9365) Arial$i, i5RNdeI(9857),EagI(9935),EagI(10100),XmaI(10323),SmaI(10325),XhoI(10340),EcoRV(10475) ArialS(i,A;5+5 i5PvuII Arial+i,j`VJVi5CɆi, APG1"Times New Romani5Ci, M13_forward20_primer"Times New Romani5Ci, M13_pUC_fwd_primer"Times New Romani5i, ORF 2"Times New Romani5Ci,#ORF 4"Times New Romani5Ci,6ORF 3"Times New Romani5Ci,ORF 1"Times New Romani5CɆi, pGEX_3_primer"Times New Romani5i,E_!KanR1"Times New Romani5CɆi,E_!ORF 1"Times New Romani5i,$&ORF 3"Times New Romani5Ci,D$E(ORF 2"Times New Romani5Ci,,., lac_promoter"Times New Romani5Ci,<,R,M13_pUC_rev_primer"Times New Romani5CɆi,Q,c,M13_reverse_primer"Times New Roman i5test"Times New Roman *Ri,T!zz+ i5test"Times New Roman:,i,iz_)