Sequence of pCAMBIA1300-VE3-2S

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Sequence of pCAMBIA1300-VE3-2S

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Sequence of pCAMBIA1300-VE3-2S: 11369 BP; 2805 A; 2947 C; 2985 G; 2632 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60

1 ttaagggcta gatcattgta tctactgtgg cgcgcgctat taaataggat caaacgcgcg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120

61 atataaaaca aaagatagcg cataatttac atattaacgc cctgagatta gtatttttgg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180

121 gtagagtatt tattgcagta cgtaatgtac aattaataat gtacgaattg cattaagttg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240

181 tctttaatat actattagta gcgttctggc cgttgtccta agttagaatt ctttgaaata 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgccaaatgt ttgaacgatc ggggaaattc gagctctcag atgggttggt ctttgggaac 300

241 acggtttaca aacttgctag cccctttaag ctcgagagtc tacccaacca gaaacccttg 300

.......... .......... .......... .......... APG1 ..........

.......... .......... .......... .......... .......... ..........

301 gatctgatcc ttgatccaca tgtagatagg gattaacaca aaccaggcag ctgctagagt 360

301 ctagactagg aactaggtgt acatctatcc ctaattgtgt ttggtccgtc gacgatctca 360

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcccaggagg aagcgtccca agaaggagaa ggggttgttg acaggcttct ctacgtcctc 420

361 agggtcctcc ttcgcagggt tcttcctctt ccccaacaac tgtccgaaga gatgcaggag 420

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttcctttgga ccaagctgga gaggagagtc accggaggca ggtttaacac cagtgacaga 480

421 aaggaaacct ggttcgacct ctcctctcag tggcctccgt ccaaattgtg gtcactgtct 480

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 acatcccatg ataaggccgt gcctgcgaac accacggtac cacttggggc caatcctctt 540

481 tgtagggtac tattccggca cggacgcttg tggtgccatg gtgaaccccg gttaggagaa 540

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gagctgaacg tccttgaaac cggcattctt gaaccactca atgtactctt cctccttggg 600

541 ctcgacttgc aggaactttg gccgtaagaa cttggtgagt tacatgagaa ggaggaaccc 600

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gaagagcatc cagacatcag aaaagaagcg agagagccag aaggttgggt agacagggcc 660

601 cttctcgtag gtctgtagtc ttttcttcgc tctctcggtc ttccaaccca tctgtcccgg 660

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gatgagacac gctttgccac cgatcttgag aaccctgtac gcttccctta ttcccctctg 720

661 ctactctgtg cgaaacggtg gctagaactc ttgggacatg cgaagggaat aaggggagac 720

.......... .......... APG1 .......... .......... ..........

.......... ScaI(735) .......... .......... .......... ..........

721 cgggtccggc cagtactcaa tgcttccagc agaaacgtat ctgtcagcat aatcggttgg 780

721 gcccaggccg gtcatgagtt acgaaggtcg tctttgcata gacagtcgta ttagccaacc 780

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aaaaggaaga tcctcagcat ctccctcgac gatcttgcat tctttcaacg gctccttttg 840

781 ttttccttct aggagtcgta gagggagctg ctagaacgta agaaagttgc cgaggaaaac 840

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctttgctttg gccagctgat gtggcgactg gtccagaatg gtcacattct tggccttcac 900

841 gaaacgaaac cggtcgacta caccgctgac caggtcttac cagtgtaaga accggaagtg 900

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtcttgact atgcccagag tagtgaaacc agttccgccg ccgacatcga ccactcgcat 960

901 acagaactga tacgggtctc atcactttgg tcaaggcggc ggctgtagct ggtgagcgta 960

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtccggatgg ctgagatccg ctggctcaag agcgtcgtct ctcatatcct cggtccaatg 1020

961 caggcctacc gactctaggc gaccgagttc tcgcagcaga gagtatagga gccaggttac 1020

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cccaggattg atgacatggt cgtatacgat ggataagaac ctgtagaacc agtaagcctc 1080

1021 gggtcctaac tactgtacca gcatatgcta cctattcttg gacatcttgg tcattcggag 1080

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cttcttgtgc tgaatgaacc taggttgcgc cgatggccgg gaagacgaca cgctgctgct 1140

1081 gaagaacacg acttacttgg atccaacgcg gctaccggcc cttctgctgt gcgacgacga 1140

.......... .......... APG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gctgcatcta gtagccaccg agagtctagg tgtggaggtt cgggtaactg agagtaaggt 1200

1141 cgacgtagat catcggtggc tctcagatcc acacctccaa gcccattgac tctcattcca 1200

.......... .......... APG1 .......... .......... ..........

EagI(1202) .......... .......... .......... .......... ..........

1201 cggccgagga atcgatctgg catgcaaatt ggaaccaggg gaacctaaac ctttggggaa 1260

1201 gccggctcct tagctagacc gtacgtttaa ccttggtccc cttggatttg gaaacccctt 1260

.......... .......... APG1 .......... .......... ..........

.......... .......... .... NcoI(1291) XmaI(1300),SmaI(1302) ....

1261 ggtaatggcc ccgttgagca tcaaagaggc catggtgacc cgggtcgtgt atgtttttaa 1320

1261 ccattaccgg ggcaactcgt agtttctccg gtaccactgg gcccagcaca tacaaaaatt 1320

.......... APG1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcttgtttgt attgatgagt tttggtttga gtaaagagtg aagcggatga gttaatttat 1380

1321 agaacaaaca taactactca aaaccaaact catttctcac ttcgcctact caattaaata 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aggctataaa ggagatttgc atggcgatca cgtgtaataa tgcatgcacg catgtgattg 1440

1381 tccgatattt cctctaaacg taccgctagt gcacattatt acgtacgtgc gtacactaac 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tatgtgtgtg ctgtgagaga gaagctctta ggtgtttgaa gggagtgaca agtggcgaag 1500

1441 atacacacac gacactctct cttcgagaat ccacaaactt ccctcactgt tcaccgcttc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaaacaatt ctccgcggct gcatgctatg tgtaacgtgt agctaatgtt ctggcatggc 1560

1501 tttttgttaa gaggcgccga cgtacgatac acattgcaca tcgattacaa gaccgtaccg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atcttatgaa cgattctttt taaaaacaag gtaaaaactt aacttcataa aattaaaaaa 1620

1561 tagaatactt gctaagaaaa atttttgttc catttttgaa ttgaagtatt ttaatttttt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaaaaacgtt tactaagttg gtttaaaagg ggatgagagt ctataaattt tggaggtagt 1680

1621 ttttttgcaa atgattcaac caaattttcc cctactctca gatatttaaa acctccatca 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gccgttggga atataaattg ggagcttaat cagaattata gaagttaaag ttgatttagt 1740

1681 cggcaaccct tatatttaac cctcgaatta gtcttaatat cttcaatttc aactaaatca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cacggtcaat ataaattggg aatttgagtc aaaatcttcc aaattcggaa tccgtcttgt 1800

1741 gtgccagtta tatttaaccc ttaaactcag ttttagaagg tttaagcctt aggcagaaca 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tacacccggt ggataggagc cgaacggttt gaaaatactt gaaatgtgga tgcaggtgca 1860

1801 atgtgggcca cctatcctcg gcttgccaaa cttttatgaa ctttacacct acgtccacgt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggctggttta attttatgtt gaatggatac atgtcaatcg aatttgagtt ataggtacac 1920

1861 ccgaccaaat taaaatacaa cttacctatg tacagttagc ttaaactcaa tatccatgtg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attttactct gatactaaaa tgtaacattt gtctcaagaa tgggtaggtc atccttactc 1980

1921 taaaatgaga ctatgatttt acattgtaaa cagagttctt acccatccag taggaatgag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gagaacggta tgccattcac ttgaacgtat atctcattca acccaactct aacacacatt 2040

1981 ctcttgccat acggtaagtg aacttgcata tagagtaagt tgggttgaga ttgtgtgtaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcatacccat gtctaaaata aagaaaattg aagaaaaacg ctcaatagcg tcaatcctta 2100

2041 agtatgggta cagattttat ttcttttaac ttctttttgc gagttatcgc agttaggaat 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 caaatataat gtacatggca acctcgcatg aaaatagtaa taaagaggag tgaataatat 2160

2101 gtttatatta catgtaccgt tggagcgtac ttttatcatt atttctcctc acttattata 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaagtagtca ctaattggat gacaaagcaa atggaaccat ccgatagatg ttttgagaac 2220

2161 tttcatcagt gattaaccta ctgtttcgtt taccttggta ggctatctac aaaactcttg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aagaggtaca tttgtatata tgcattggtt gatacatttc tgccatctga attgtttctt 2280

2221 ttctccatgt aaacatatat acgtaaccaa ctatgtaaag acggtagact taacaaagaa 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cctcaaagaa gaagcattca gccaatagtg aaatttaaaa tgttggaaaa atgaggatgc 2340

2281 ggagtttctt cttcgtaagt cggttatcac tttaaatttt acaacctttt tactcctacg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agtcagaaag tccagaatat aagtggtaac tggtaactga atacttgcct cagaagcaag 2400

2341 tcagtctttc aggtcttata ttcaccattg accattgact tatgaacgga gtcttcgttc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ........ HindIII(2445) ........

2401 ataagaaaca taagtcttta aaggaatcaa tcaccgatga agaaagcttc tgcaggcatg 2460

2401 tattctttgt attcagaaat ttccttagtt agtggctact tctttcgaag acgtccgtac 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 2520

2461 gttcgaaccg tgaccggcag caaaatgttg cagcactgac ccttttggga ccgcaatggg 2520

..... M13_forward20_primer . M13_pUC_fwd_primer ...... ..........

.......... .......... .......... .......... .......... ..........

2521 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 2580

2521 ttgaattagc ggaacgtcgt gtagggggaa agcggtcgac cgcattatcg cttctccggg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga 2640

2581 cgtggctagc gggaagggtt gtcaacgcgt cggacttacc gcttacgatc tcgtcgaact 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... PmeI(2676) .......... ..........

2641 gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat 2700

2641 cgaacctagt ctaacagcaa agggcggaag tcaaatttga tagtcacaaa ctgtcctata 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc 2760

2701 taaccgccca tttggattct cttttctcgc aaataatctt attgcctata aattttcccg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg 2820

2761 cacttttcca aataggcaag caggtaaaca tacacgtacg gttggtgtcc caaggggagc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc 2880

2821 cctagtttca tgaaactagg ttggggaggc gacgatatca cgtcagccga agactgcaag 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc 2940

2881 tcacgtcggc agaagacttt tgctgtacag cgtgttcagg attcaatgcg ctgtccgacg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac 3000

2941 gcgggacggg aaaaggaccg caaaagaaca gcgcacaaaa tcagcgtatt tcatcttatg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg 3060

3001 aacgctgatc ttggcctctg taatgcggta cttgttctcg cggcggcgac cggacgaccc 3060

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

3061 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc 3120

3061 gatacgggcg cagtcgtggc tgctggtcct gaactggttg gttgcccggc ttgacgtgcg 3120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga 3180

3121 ccggccgacg tggttcgaca aaaggctctt ctagtggccg tggtccgcgc tggcgggcct 3180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga 3240

3181 cgaccggtcc tacgaactgg tggatgcggg accgctgcaa cactgtcact ggtccgatct 3240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg 3300

3241 ggcggaccgg gcgtcgtggg cgctggatga cctgtaacgg ctcgcgtagg tcctccggcc 3300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat 3360

3301 gcgcccggac gcatcggacc gtctcggcac ccggctgtgg tggtgcggcc ggccggcgta 3360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac 3420

3361 ccacaactgg cacaagcggc cgtaacggct caagctcgca agggattagt agctggcgtg 3420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... SfiI(3444) .......... .......... ..........

3421 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct 3480

3421 ggcctcgccc gcgctccggc ggttccgggc tccgcacttc aaaccggggg cgggatggga 3480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa 3540

3481 gtggggccgt gtctagcgcg tgcgggcgct cgactagctg gtccttccgg cgtggcactt 3540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag 3600

3541 tctccgccga cgtgacgaac cgcacgtagc gagctgggac atggcgcgtg aactcgcgtc 3600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac 3660

3601 gctccttcac tgcgggtggc tccggtccgc cgcgccacgg aaggcactcc tgcgtaactg 3660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg 3720

3661 gctccggctg cgggaccgcc ggcggctctt acttgcggtt ctccttgttc gtactttggc 3720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc 3780

3721 gtggtcctgc cggtcctgct tggcaaaaag taatggcttc tctagctccg cctctactag 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc 3840

3781 cgccggccca tgcacaagct cggcgggcgc gtgcagagtt ggcacgccga cgtactttag 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SfiI(3878) .......... ..........

3841 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa 3900

3841 gaccggccaa acagactacg gttcgaccgc cggaccggcc ggtcgaaccg gcgacttctt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg 3960

3901 tggctcgcgg cggcagattt ttccactaca cataaactca ttttgtcgaa cgcagtacgc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg 4020

3961 cagcgacgca tatactacgc tactcattta tttgtttatg cgttcccctt gcgtacttcc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag 4080

4021 aatagcgaca tgaattggtc tttccgccca gtccgttctg ctggtagcgt tgggtagatc 4080

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca 4140

4081 gggcgcggga cgttgagcgg ccccggctac aagacaatca gctaaggcta ggggtcccgt 4140

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc 4200

4141 cacgggcgct aacccgccgg cacgcccttc tagttggcga ttggcaacag ccgtagctgg 4200

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg 4260

4201 cgggctgcta actggcgctg cacttccggt agccggccgc gctgaagcat cactagctgc 4260

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga 4320

4261 ctcgcggggt ccgccgcctg aaccgacaca ggcgctagtt ccgtcggctg aagcacgact 4320

.......... .......... ORF 4 .......... .......... ..........

.......... .......... NdeI(4348) ......... SexAI(4364) .........

4321 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta 4380

4321 aaggccacgt cggttcggga atgctgtata cccggtggcg gctggaccac ctcgaccaat 4380

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga 4440

4381 tcgtcgcgta actccagtgc ctaccttccg atgttcgccg gaaacagcac agcgcccgct 4440

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca 4500

4441 agtttccgtg cgcgtagccg ccactccaac ggctccgcga ccggcccatg ctcgacgggt 4500

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa 4560

4501 aagaactcag ggcatagtgc gtcgcgcact cgatgggtcc gtgacggcgg cggccgtgtt 4560

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg 4620

4561 ggcaagaact tagtcttggg ctcccgctgc gacgggcgct ccaggtccgc gaccggcgac 4620

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa 4680

4621 tttaatttag ttttgagtaa actcaattac tccatttctc ttttactcgt tttcgtgttt 4680

.......... ORF 4 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct 4740

4681 gtgcgattca cggccggcag gctcgcgtgc gtcgtcgttc cgacgttgca accggtcgga 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa 4800

4741 ccgtctgtgc ggtcggtact tcgcccagtt gaaagtcaac ggccgcctcc tagtgtggtt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat 4860

4801 cgacttctac atgcgccatg cggttccgtt ctggtaatgg ctcgacgata gacttatgta 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta 4920

4861 gcgcgtcgat ggtctcattt actcgtttac ttatttactc atctacttaa aatcgccgat 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt 4980

4921 ttcctccgcc gtacctttta gttcttgttg gtccgtggct gcggcacctt acggggtaca 4980

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg 5040

4981 cacctccttg cccgccaacc ggtccgcatt cgccgaccca acagacggcc gggacgttac 5040

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta 5100

5041 cgtgaccttg ggggttcggg ctccttagcc gcactgccag cgtttggtag gccgggccat 5100

.......... ......... ORF 3 ORF 1 ......... .......... ..........

.......... ......... SexAI(5127) ......... .......... ..........

5101 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc 5160

5101 gtttagccgc gccgcgaccc actactggac cacctcttca acttccggcg cgtccggcgg 5160

.......... ......... ORF 3 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .... NotI(5211),EagI(5211)

5161 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat 5220

5161 gtcgccgttg cgtagctccg tcttcgtgcg gggccactta gcaccgttcg ccggcgacta 5220

.......... ......... ORF 3 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg 5280

5221 gcttaggcgt ttcttagggc cgttggcggc cgtcggccac gcggcagcta atccttcggc 5280

.......... ......... ORF 3 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc 5340

5281 gggttcccgc tgctcgttgg tctaaaaaag caaggctacg agatactgca cccgtgggcg 5340

.......... ......... ORF 3 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct 5400

5341 ctatcagcgt cgtagtacct gcaccggcaa aaggcagaca gcttcgcact ggctgctcga 5400

.......... ......... ORF 3 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc 5460

5401 ccgctccact aggcgatgct cgaaggtctg cccgtgcatc tccaaaggcg tcccggccgg 5460

.......... ......... ORF 3 ORF 1 ......... .......... ..........

SfiI(5465) .......... .......... .......... .......... ..........

5461 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa 5520

5461 ccgtaccggt cacacaccct aatgctggac catgactacc gccaaagggt agattggctt 5520

.......... ......... ORF 3 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac 5580

5521 aggtacttgg ctatggccct tcccttccct ctgttcgggc cggcgcacaa ggcaggtgtg 5580

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg 5640

5581 caacgcctgc atgagttcaa gacggccgct cggctaccgc ctttcgtctt tctgctggac 5640

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc 5700

5641 catctttgga cgtaagccaa tttgtggtgc gtgcaacggt acgtcgcatg cttcttccgg 5700

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc 5760

5701 ttcttgccgg cggaccactg ccataggctc ccacttcgga actaatcggc gatgttctag 5760

.......... .......... ORF 1 .......... .......... ..........

.......... EagI(5780) .......... .......... .......... ..........

5761 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac 5820

5761 catttctcgc tttggcccgc cggcctcatg tagctctagc tcgatcgact aacctacatg 5820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg 5880

5821 gcgctctagt gtcttccgtt cttgggcctg cacgactgcc aagtggggct aatgaaaaac 5880

.......... .......... ORF 1 .......... .......... ..........

ClaI(5883) EagI(5896) .......... .......... .......... ..........

5881 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca 5940

5881 tagctagggc cgtagccggc aaaagagatg gcggaccgtg cggcgcggcg tccgttccgt 5940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag 6000

5941 cttcggtcta ccaacaagtt ctgctagatg cttgcgtcac cgtcgcggcc tctcaagttc 6000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg 6060

6001 ttcaagacaa agtggcacgc gttcgactag cccagtttac tggacggcct catgctaaac 6060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag 6120

6061 ttcctcctcc gccccgtccg accgggctag gatcagtacg cgatggcgtt ggactagctc 6120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca 6180

6121 ccgcttcgta ggcggccaag gattacatgc ctcgtctacg atcccgttta acgggatcgt 6180

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag 6240

6181 cccctttttc cagcttttcc agagaaagga cacctatcgt gcatgtaacc cttgggtttc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg 6300

6241 ggcatgtaac ccttggcctt gggcatgtaa cccttgggtt tcggcatgta acccttggcc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct 6360

6301 agtgtgtaca ttcactgact atattttctc ttttttccgc taaaaaggcg gattttgaga 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc 6420

6361 aattttgaat aattttgaga attttgggcg gaccggacac gtattgacag accggtcgcg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc 6480

6421 tgtcggcttc tcgacgtttt tcgcggatgg gaagccagcg acgcgaggga tgcggggcgg 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc 6540

6481 cgaagcgcag ccggatagcg ccggcgaccg gcgagttttt accgaccgga tgccggtccg 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa 6600

6541 ttagatggtc ccgcgcctgt tcggcgcggc agcggtgagc tggcggccgc gggtgtagtt 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 6660

6601 ccgtgggacg gagcgcgcaa agccactact gccacttttg gagactgtgt acgtcgaggg 6660

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

6661 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 6720

6661 cctctgccag tgtcgaacag acattcgcct acggccctcg tctgttcggg cagtcccgcg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 6780

6721 cagtcgccca caaccgccca cagccccgcg tcggtactgg gtcagtgcat cgctatcgcc 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NdeI(6837)

6781 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 6840

6781 tcacatatga ccgaattgat acgccgtagt ctcgtctaac atgactctca cgtggtatac 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 6900

6841 gccacacttt atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc gagaaggcga 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6960

6901 aggagcgagt gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7020

6961 agtttccgcc attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7080

7021 cgttttccgg tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7140

7081 tccgaggcgg ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7200

7141 ggctgtcctg atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7260

7201 caaggctggg acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7320

7261 gaaagagtat cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7380

7321 ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7440

7381 gaactcaggt tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7500

7441 taatcgtctc gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7560

7501 ccgatgtgat cttcctgtca taaaccatag acgcgagacg acttcggtca atggaagcct 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7620

7561 ttttctcaac catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7680

7621 caaacgttcg tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat 7740

7681 agatgcccca gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtacgta 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc 7800

7741 agatccatga ttttgttaag taggtcattt tatattataa aataaaagag ggttagtccg 7800

.......... .......... ......... KanR1 ORF 1 ......... ..........

.......... .......... .......... .......... ......... EcoRV(7851)

7801 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg 7860

7801 aactaggggt cattcagttt tttatcgagc tgtatgacaa gaaggggcta taggagggac 7860

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga 7920

7861 tagctggcct gcgtcttccg ttacagtatg gtgaacaggc gggacggcga agagggttct 7920

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg 7980

7921 agttatttcg gtgaatgaaa cggtagaaag tgtttctaca acgacagagg gtccagcggc 7980

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc 8040

7981 acccttttct gttcaaggag aagcccgaaa aggcagaaat tttttagtat gtcgagcgcg 8040

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta 8100

8041 cctagaaatt tacctcacag aagaagggtc aaaagcgtta ggtgtagccg gtctagcaat 8100

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc 8160

8101 aagtcattca ttaggttaag ccgattcgcc gacagattcg ataagcatat ccctgttagg 8160

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca 8220

8161 ctatacagct acctcacttt ctcggactac gtgaggcgta tgtcgagcta ttagaaaagt 8220

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc 8280

8221 cccgaaacaa gtagaagtat gagaaggctc gtttcctgcg gtagccggag tgagtactcg 8280

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct 8340

8281 tctaacgagg tcggtagtac ggcaagtttc acgtcctgga aaccttgtcc gtcgaaagga 8340

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg 8400

8341 aggtcggtat cgtagtacag gaaaagggca aggtgtagta tccaccaggg aaatatggcc 8400

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca 8460

8401 gacaggcagt aaaaatttat atccaaaagt aaaagagggt ggtcgaatat atggaatcgt 8460

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc 8520

8461 cctctgtaag gaaggcatag aaaatgcgtc gccataaaaa gctagtcaaa aaagttaagg 8520

.......... ......... KanR1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga 8580

8521 ccactataag agtaaaatcg gtaaataata aaggaaggag aaaagatgtc ataaatttct 8580

......... KanR1 ORF 1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa 8640

8581 atggggttct tcgattaata ttgttctgct tgaggttaag tgacaaggaa cgtaagattt 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag 8700

8641 tggaatttat ggtcttttgt cgaaaaagtt tcaacaaaag tttcaaccgc atattgtatc 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt 8760

8701 atagctgcct cggctaaaac tttggcgcca ctagtgtccg tcgttgcgag acagtagcaa 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc 8820

8761 tgttagttgt acgatgggag gcgctctagt aggcacaaag tttgggccgt cgaatcaacg 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc 8880

8821 gcaagaaggc ttatcgtagc cattgtactc gtttcagacg gcggaatgtt gccgagaggg 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg 8940

8881 cgactgcggc agggcctgac tacccgacgg acatagctca ccactaaaac acggctcgac 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac 9000

8941 ggccagcccc tcgacaaccg accgaccacc gtcctatata acaccacatt tgtttaactg 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt 9060

9001 cgaatctgtt gaattattgt gtaacgcctg caaaaattac atgacttaat tgcggcttaa 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg 9120

9061 ttaagccccc tagacctaaa atcatgacct aaaaccaaaa tccttaatct ttaaaataac 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga 9180

9121 tatcttcata aaatgtttat gtttatgtat gattcccaaa gaatatacga gttgtgtact 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga 9240

9181 cgctttggga tatccttggg attaagggaa tagacccttg atgagtgtgt aataatacct 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg 9300

9241 ctttgagctc gaacagctag ctgtctaggc cagccgtaga tgagataaag aaacgggagc 9300

.......... .......... ORF 3 .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

9301 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc 9360

9301 ctgctcacga ccccgcagcc aaaggtgata gccgctcatg aagatgtgtc ggtagccagg 9360

.......... ......... ORF 3 ORF 2 ......... .......... ..........

EagI(9365) .......... .......... .......... .......... ..........

9361 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga 9420

9361 tctgccggcg cgaagacgcc cgctaaacac atgcgggctg tcagggccga ggcctagcct 9420

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc 9480

9421 gctaacgcag cgtagctggg acgcgggttc gacgtagtag ctttaacggc agttggttcg 9480

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg 9540

9481 agactatctc aaccagttct ggttacgcct cgtatatgcg ggcctcagca ccgctaggac 9540

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg 9600

9541 gttcgaggcc tacggaggcg agcttcatcg cgcagacgac gaggtatgtt cggttggtgc 9600

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc 9660

9601 cggaggtctt cttctacaac cgctggagca taacccttag gggcttgtag cggagcgagg 9660

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg 9720

9661 tcagttactg gcgacaatac gccggtaaca ggcagtcctg taacaacctc ggctttaggc 9720

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag 9780

9721 gcacgtgctc cacggcctga agccccgtca ggagccgggt ttcgtagtcg agtagctctc 9780

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg 9840

9781 ggacgcgctg cctgcgtgac tgccacagca ggtagtgtca aacggtcact atgtgtaccc 9840

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc 9900

9841 ctagtcgtta gcgcgtatac tttagtgcgg tacatcacat aactggctaa ggaacgccag 9900

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct 9960

9901 gcttacccgg cttgggcgag cagaccgatt ctagccggcg tcgctagcgt aggtatcgga 9960

.......... ......... ORF 3 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac 10020

9961 ggcgctggcc aacatcttgt cgcccgtcaa gccaaagtcc gtccagaacg ttgcactgtg 10020

ORF 3 .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca 10080

10021 ggacacgtgc cgccctctac gttatccagt ccgagagcga tttgaggggt tacagttcgt 10080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga 10140

10081 gaaggcctta gccctcgcgc cggctacgtt tcacggctat ttgtattgct agaaacatct 10140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga 10200

10141 ttggtagccg cgtcgataaa tgggcgtcct gtataggtgc gggaggatgt agcttcgact 10200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt 10260

10201 ttcgtgctct aagaagcggg aggctctcga cgtagtccag cctctgcgac agcttgaaaa 10260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc 10320

10261 gctagtcttt gaagagctgt ctgcagcgcc actcaagtcc gaaaaagtat agagtaacgg 10320

.......... .......... ORF 2 .......... .......... ..........

XmaI(10323),SmaI(10325) ...... .......... .......... ..........

10321 ccccgggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag 10380

10321 ggggccctag acgctttcga gctctctcta tctaaacatc tctctctgac cactaaagtc 10380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt 10440

10381 gcacaggaga ggtttacttt acttgaagga atatatctcc ttccagaacg cttcctatca 10440

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRV(10475) ......... ..........

10441 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag 10500

10441 ccctaacacg cagtagggaa tgcagtcacc tctatagtgt agttaggtga acgaaacttc 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg 10560

10501 tgcaccaacc ttgcagaaga aaaaggtgct acgaggagca cccaccccca ggtagaaacc 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt 10620

10561 ctggtgacag ccgtctccgt agaacttgct atcggaaagg aaatagcgtt actaccgtaa 10620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat 10680

10621 acatccacgg tggaaggaaa agatgacagg aaaactactt cactgtctat cgacccgtta 10680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt 10740

10681 ccttaggctc ctccaaaggg ctataatggg aaacaacttt tcagagttat cgggaaacca 10740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt 10800

10741 gaagactctg acatagaaac tataagaacc tcatctgctc tcacagcacg aggtggtaca 10800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc 10860

10801 atagtgtagt taggtgaacg aaacttctgc accaaccttg cagaagaaaa aggtgctacg 10860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag 10920

10861 aggagcaccc acccccaggt agaaaccctg gtgacagccg tctccgtaga acttgctatc 10920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt 10980

10921 ggaaaggaaa tagcgttact accgtaaaca tccacggtgg aaggaaaaga tgacaggaaa 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt 11040

10981 actacttcac tgtctatcga cccgttacct taggctcctc caaagggcta taatgggaaa 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt 11100

11041 caacttttca gagttatcgg gaaaccagaa gactctgaca tagaaactat aagaacctca 11100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11101 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc 11160

11101 tctgctctca cagcacgagg tggtacaacc gttcgacgag atcggttatg cgtttggcgg 11160

.......... .......... .......... .......... .......... ..........

.......... .......... ......... PvuII(11194) ......... ..........

11161 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 11220

11161 agaggggcgc gcaaccggct aagtaattac gtcgaccgtg ctgtccaaag ggctgacctt 11220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11221 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 11280

11221 tcgcccgtca ctcgcgttgc gttaattaca ctcaatcgag tgagtaatcc gtggggtccg 11280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 11340

11281 aaatgtgaaa tacgaaggcc gagcatacaa cacaccttaa cactcgccta ttgttaaagt 11340

......... lac_promoter ......... .......... ...... M13_pUC_rev_primer

.......... .......... .......... .......... .......... ..........

11341 cacaggaaac agctatgacc atgattacg. .......... .......... .......... 11369

11341 gtgtcctttg tcgatactgg tactaatgc. .......... .......... .......... 11369

...... M13_reverse_primer ...... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-11369 Reference author: Shi X.G., Zhuang W.J.; Reference title: "Binary vector pCAMBIA1300-VE3-2S for making MPBQ/MSBQ methyltransferase Reference title: over expression in seed"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-11369 Reference author: Zhuang W.J., Shi X.G.; Reference title: ; Reference location: Submitted (08-OCT-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Crop Science Department, Oil Crop Institution, Fujian Agriculture, Jinshan, Reference location: Fuzhou, Fujian 350002, P.R. China