ttJLOCUS pCAT3-Basic 4027 bp DNA SYN 28-Aug-2007 DEFINITION pCAT3-Basic ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4027 /organism="pCAT3-Basic" /mol_type="other DNA" misc_feature 103..235 /label="chim_int" CDS 291..950 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 3" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 291..950 /label="CAT/CamR" /gene="CAT/CamR" misc_feature complement(1001..1020) /label="EBV_rev_primer" terminator complement(987..1106) /label="SV40_PA_terminator" rep_origin complement(1515..2134) /label="pBR322_origin" CDS complement(2289..3149) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2289..3149) /label="Ampicillin" /gene="Ampicillin" promoter complement(3191..3219) /label="AmpR_promoter" rep_origin 3298..3604 /label="f1_origin" terminator 3867..3915 /label="synth_PA_terminator" misc_feature 3867..4020 /label="TB" terminator 3867..4020 /label="up_PA_terminator" misc_feature 3969..3988 /label="RVprimer3_primer" ORIGIN 1 GGTACCGAGC TCTTACGCGT GCTAGCCCGG GCTCGAGATC TGCGATCTAA GTAAGCTTGC 61 GGCCGCTTAA GCTGCAGAAG TTGGTCGTGA GGCACTGGGC AGGTAAGTAT CAAGGTTACA 121 AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT 181 TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC 241 CACTCCCAGG TTCAATACAG CTCTTAAGCG GCCGCAAGCT TGCCGCCACC ATGGAGAAAA 301 AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG 361 CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT 421 TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG 481 CCCGCCTGAT GAATGCTCAT CCGGAACTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA 541 TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT 601 CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG 661 TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT 721 TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG 781 ACAACTTCTT CGCCCCCGTT TTCACGATGG GCAAATATTA TACGCAAGGC GACAAGGTGC 841 TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA 901 TGCTTAATGA ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA TCTAGAGTCG 961 GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC 1021 AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT 1081 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT 1141 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG 1201 TAAAATCGAT AAGGATCCGT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT 1261 TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC 1321 AACTCGTAGG ACAGGTGCCG GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC 1381 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 1441 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 1501 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 1561 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 1621 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 1681 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 1741 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 1801 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 1861 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 1921 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 1981 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 2041 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 2101 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 2161 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 2221 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 2281 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 2341 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 2401 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA 2461 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 2521 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 2581 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 2641 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 2701 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 2761 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 2821 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 2881 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 2941 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 3001 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 3061 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 3121 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 3181 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 3241 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 3301 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 3361 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 3421 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 3481 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 3541 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 3601 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 3661 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA 3721 TTAACGCTTA CAATTTGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG 3781 GTGCGGGCCT CTTCGCTATT ACGCCAGCCC AAGCTACCAT GATAAGTAAG TAATATTAAG 3841 GTACGGGAGG TACTTGGAGC GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG 3901 TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC 3961 AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT 4021 ATCGATA // pCAT3-Basic "Times New RomantCgchim_int"Times New RomantTB"Times New RomantC up_PA_term"Times New Roman(tC#ORF 3"Times New RomantCɆ#CAT/CamR"Times New RomantCR SV40_PA_term"Times New Romant EBV_rev_pri"Times New RomantCV pBR322_origin"Times New RomantCɆM ORF 3"Times New RomantM  Ampicillin"Times New RomantCw AmpR_pro"Times New RomantC  f1_origin"Times New RomantCɆK synth_PA_term"Times New RomantC RVpri3_pri"Times New Roman t>KpnI(5),SacI(11),NheI(21),SmaI(28),XhoI(32),BglII(36),PstI(76)"Times New Roman~~ tNcoI(289),PvuII(404)"Times New Roman!#,::z tMscI"Times New Roman;4,%3 tXbaI"Times New Romanyxvo} t!HpaI(1111),BamHI(1213),SalI(1219)"Times New RomanW tAseI"Times New Roman '5BBQ ttest"Times New Roman]Fd ttest"Times New Roman*d