Sequence of pCAT3-Basic

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Sequence of pCAT3-Basic

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Sequence of pCAT3-Basic : 4027 BP; 1024 A; 979 C; 953 G; 1071 T; 0 other;

KpnI(5) SacI(11) NheI(21) .......... .......... ..........

1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgc 60

1 ccatggctcg agaatgcgca cgatcgggcc cgagctctag acgctagatt cattcgaacg 60

.......... .......... .......... .......... .......... ..........

.......... PstI(76) .......... .......... .......... ..........

61 ggccgcttaa gctgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca 120

61 ccggcgaatt cgacgtcttc aaccagcact ccgtgacccg tccattcata gttccaatgt 120

.......... .......... .......... .......... .......... chim_int

.......... .......... .......... .......... .......... ..........

121 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt 180

121 tctgtccaaa ttcctctggt tatctttgac ccgaacagct ctgtctcttc tgagaacgca 180

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc 240

181 aagactatcc gtggataacc agaatgactg taggtgaaac ggaaagagag gtgtccacag 240

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cactcccagg ttcaatacag ctcttaagcg gccgcaagct tgccgccacc atggagaaaa 300

241 gtgagggtcc aagttatgtc gagaattcgc cggcgttcga acggcggtgg tacctctttt 300

.......... .......... .......... .......... ........ ORF 3 CAT/CamR

.......... .......... .......... .......... .......... ..........

301 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 360

301 tttagtgacc tatatggtgg caactatata gggttaccgt agcatttctt gtaaaactcc 360

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... PvuII(404) ..........

361 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 420

361 gtaaagtcag tcaacgagtt acatggatat tggtctggca agtcgaccta taatgccgga 420

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 480

421 aaaatttctg gcatttcttt ttattcgtgt tcaaaatagg ccggaaataa gtgtaagaac 480

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 cccgcctgat gaatgctcat ccggaactcc gtatggcaat gaaagacggt gagctggtga 540

481 gggcggacta cttacgagta ggccttgagg cataccgtta ctttctgcca ctcgaccact 540

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 600

541 ataccctatc acaagtggga acaatgtggc aaaaggtact cgtttgactt tgcaaaagta 600

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 660

601 gcgagacctc acttatggtg ctgctaaagg ccgtcaaaga tgtgtatata agcgttctac 660

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 720

661 accgcacaat gccacttttg gaccggataa agggatttcc caaataactc ttatacaaaa 720

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... MscI(771)

721 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 780

721 agcagagtcg gttagggacc cactcaaagt ggtcaaaact aaatttgcac cggttatacc 780

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaacttctt cgcccccgtt ttcacgatgg gcaaatatta tacgcaaggc gacaaggtgc 840

781 tgttgaagaa gcgggggcaa aagtgctacc cgtttataat atgcgttccg ctgttccacg 840

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa 900

841 actacggcga ccgctaagtc caagtagtac ggcaaacact accgaaggta cagccgtctt 900

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... XbaI(951)

901 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tctagagtcg 960

901 acgaattact taatgttgtc atgacgctac tcaccgtccc gccccgcatt agatctcagc 960

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac 1020

961 cccgccggcc ggcgaagctc gtctgtacta ttctatgtaa ctactcaaac ctgtttggtg 1020

.......... .......... .......... ... SV40_PA_term EBV_rev_pri ...

.......... .......... .......... .......... .......... ..........

1021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 1080

1021 ttgatcttac gtcacttttt ttacgaaata aacactttaa acactacgat aacgaaataa 1080

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 1140

1081 acattggtaa tattcgacgt tatttgttca attgttgttg ttaacgtaag taaaatacaa 1140

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 1200

1141 agtccaagtc cccctccaca ccctccaaaa aatttcgttc attttggaga tgtttacacc 1200

.......... .......... .......... .......... .......... ..........

.... BamHI(1213) SalI(1219) .... .......... .......... ..........

1201 taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 1260

1201 attttagcta ttcctaggca gctggctacg ggaactctcg gaagttgggt cagtcgagga 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 1320

1261 aggccacccg cgccccgtac tgatagcagc ggcgtgaata ctgacagaag aaatagtacg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc 1380

1321 ttgagcatcc tgtccacggc cgtcgcgaga aggcgaagga gcgagtgact gagcgacgcg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 1440

1381 agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 1500

1441 tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 1560

1501 ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta 1560

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 1620

1561 gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 1680

1621 cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 1740

1681 atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 1800

1741 atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 1860

1801 gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 1920

1861 ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 1980

1921 ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc ttgtcataaa 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2040

1981 ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2100

2041 ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2160

2101 tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc 2160

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 2220

2161 ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta gaagtggatc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 2280

2221 taggaaaatt taatttttac ttcaaaattt agttagattt catatatact catttgaacc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 2340

2281 agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac agataaagca 2340

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2341 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 2400

2341 agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct cccgaatggt 2400

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 2460

2401 agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg tctaaatagt 2460

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 2520

2461 cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg aaataggcgg 2520

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... AseI(2534) .......... .......... .......... ..........

2521 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 2580

2521 aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg tcaattatca 2580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 2640

2581 aacgcgttgc aacaacggta acgatgtccg tagcaccaca gtgcgagcag caaaccatac 2640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 2700

2641 cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg gtacaacacg 2700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 2760

2701 ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa ccggcgtcac 2760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 2820

2761 aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg taggcattct 2820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 2880

2821 acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac atacgccgct 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 2940

2881 ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc gtcttgaaat 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3000

2941 tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta gaatggcgac 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3060

3001 aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg tagaaaatga 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 3120

3061 aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt tttcccttat 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 3180

3121 tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat aacttcgtaa 3180

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 3240

3181 atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt tttatttgtt 3240

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 3300

3241 tatccccaag gcgcgtgtaa aggggctttt cacggtggac tgcgcgggac atcgccgcgt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 3360

3301 aattcgcgcc gcccacacca ccaatgcgcg tcgcactggc gatgtgaacg gtcgcgggat 3360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 3420

3361 cgcgggcgag gaaagcgaaa gaagggaagg aaagagcggt gcaagcggcc gaaaggggca 3420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 3480

3421 gttcgagatt tagcccccga gggaaatccc aaggctaaat cacgaaatgc cgtggagctg 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 3540

3481 gggttttttg aactaatccc actaccaagt gcatcacccg gtagcgggac tatctgccaa 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 3600

3541 aaagcgggaa actgcaacct caggtgcaag aaattatcac ctgagaacaa ggtttgacct 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 3660

3601 tgttgtgagt tgggatagag ccagataaga aaactaaata ttccctaaaa cggctaaagc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 3720

3661 cggataacca attttttact cgactaaatt gtttttaaat tgcgcttaaa attgttttat 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 3780

3721 aattgcgaat gttaaacggt aagcggtaag tccgacgcgt tgacaaccct tcccgctagc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag 3840

3781 cacgcccgga gaagcgataa tgcggtcggg ttcgatggta ctattcattc attataattc 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg 3900

3841 catgccctcc atgaacctcg ccggcgttat tttatagaaa taaaagtaat gtagacacac 3900

.......... .......... .......... ........ synth_PA_term ........

.......... .......... .......... .......... .......... ..........

3901 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac 3960

3901 aaccaaaaaa cacacttagc tatcatgatt gtatgcgaga ggtagttttg ttttgctttg 3960

synth_PA_term ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct 4020

3961 ttttgtttga tcgttttatc cgacaggggt cacgttcacg tccacggtct tgtaaagaga 4020

.......... RVpri3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atcgata... .......... .......... .......... .......... .......... 4027

4021 tagctat... .......... .......... .......... .......... .......... 4027

.......... .......... .......... .......... .......... ..........