Sequence of pCAT3-Promoter

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Sequence of pCAT3-Promoter

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Sequence of pCAT3-Promoter : 4219 BP; 1060 A; 1046 C; 992 G; 1121 T; 0 other;

KpnI SacI11) NheI(21) SmaI(28),XhoI(32),BglII(36) ..

1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta 60

1 ccatggctcg agaatgcgca cgatcgggcc cgagctctag acgctagacg tagagttaat 60

.......... .......... .......... .......... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgc ccagttc 120

61 cagtcgttgg tatcagggcg gggattgagg cgggtagggc ggggattgag gcgggtcaag 120

.......... .......... SV40_pro ......... SV40_origin .........

.......... .......... .......... .......... .......... ..........

121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc 180

121 gcgggtaaga ggcggggtag cgactgatta aaaaaaataa atacgtctcc ggctccggcg 180

.......... ..... SV40_origin SV40_pro ... SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 240

181 gagccggaga ctcgataagg tcttcatcac tcctccgaaa aaacctccgg atccgaaaac 240

.......... .......... SV40_pro .......... .......... ..........

.......... .......... PstI(268) .......... .......... ..........

241 caaaaagctt gcggccgctt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt 300

241 gtttttcgaa cgccggcgaa ttcgacgtct tcaaccagca ctccgtgacc cgtccattca 300

SV40_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga 360

301 tagttccaat gttctgtcca aattcctctg gttatctttg acccgaacag ctctgtctct 360

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc 420

361 tctgagaacg caaagactat ccgtggataa ccagaatgac tgtaggtgaa acggaaagag 420

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tccacaggtg tccactccca ggttcaatac agctcttaag cggccgcaag cttgccgcca 480

421 aggtgtccac aggtgagggt ccaagttatg tcgagaattc gccggcgttc gaacggcggt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ccatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag 540

481 ggtacctctt tttttagtga cctatatggt ggcaactata tagggttacc gtagcatttc 540

.......... .......... ........ ORF 3 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... PvuII(596)

541 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg 600

541 ttgtaaaact ccgtaaagtc agtcaacgag ttacatggat attggtctgg caagtcgacc 600

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta 660

601 tataatgccg gaaaaatttc tggcatttct ttttattcgt gttcaaaata ggccggaaat 660

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttcacattct tgcccgcctg atgaatgctc atccggaact ccgtatggca atgaaagacg 720

661 aagtgtaaga acgggcggac tacttacgag taggccttga ggcataccgt tactttctgc 720

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg 780

721 cactcgacca ctatacccta tcacaagtgg gaacaatgtg gcaaaaggta ctcgtttgac 780

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat 840

781 tttgcaaaag tagcgagacc tcacttatgg tgctgctaaa ggccgtcaaa gatgtgtata 840

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg 900

841 taagcgttct acaccgcaca atgccacttt tggaccggat aaagggattt cccaaataac 900

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg 960

901 tcttatacaa aaagcagagt cggttaggga cccactcaaa gtggtcaaaa ctaaatttgc 960

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

MscI(963) .......... .......... .......... .......... ..........

961 tggccaatat ggacaacttc ttcgcccccg ttttcacgat gggcaaatat tatacgcaag 1020

961 accggttata cctgttgaag aagcgggggc aaaagtgcta cccgtttata atatgcgttc 1020

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc 1080

1021 cgctgttcca cgactacggc gaccgctaag tccaagtagt acggcaaaca ctaccgaagg 1080

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt 1140

1081 tacagccgtc ttacgaatta cttaatgttg tcatgacgct actcaccgtc ccgccccgca 1140

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

XbaI(1143) .......... .......... .......... .......... ..........

1141 aatctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt 1200

1141 ttagatctca gccccgccgg ccggcgaagc tcgtctgtac tattctatgt aactactcaa 1200

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

1201 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc 1260

1201 acctgtttgg tgttgatctt acgtcacttt ttttacgaaa taaacacttt aaacactacg 1260

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat 1320

1261 ataacgaaat aaacattggt aatattcgac gttatttgtt caattgttgt tgttaacgta 1320

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct 1380

1321 agtaaaatac aaagtccaag tccccctcca caccctccaa aaaatttcgt tcattttgga 1380

.......... .......... .......... .......... .......... ..........

.......... ......... BamHI(1405) SalI(1411) ......... ..........

1381 ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga gccttcaacc 1440

1381 gatgtttaca ccattttagc tattcctagg cagctggcta cgggaactct cggaagttgg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct 1500

1441 gtcagtcgag gaaggccacc cgcgccccgt actgatagca gcggcgtgaa tactgacaga 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact 1560

1501 agaaatagta cgttgagcat cctgtccacg gccgtcgcga gaaggcgaag gagcgagtga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 1620

1561 ctgagcgacg cgagccagca agccgacgcc gctcgccata gtcgagtgag tttccgccat 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 1680

1621 tatgccaata ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 1740

1681 gttttccggt ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg 1740

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1741 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 1800

1741 ggactgctcg tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 1860

1801 atttctatgg tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 1920

1861 ggcgaatggc ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagtatcg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 1980

1921 agtgcgacat ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 2040

1981 cttggggggc aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 2100

2041 ggccattctg tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 2160

2101 tccatacatc cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 2220

2161 tcttgtcata aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 2280

2221 tcgagaacta ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 2340

2281 gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 2400

2341 ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc agtactctaa tagtttttcc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 2460

2401 tagaagtgga tctaggaaaa tttaattttt acttcaaaat ttagttagat ttcatatata 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 2520

2461 ctcatttgaa ccagactgtc aatggttacg aattagtcac tccgtggata gagtcgctag 2520

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2521 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 2580

2521 acagataaag caagtaggta tcaacggact gaggggcagc acatctattg atgctatgcc 2580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 2640

2581 ctcccgaatg gtagaccggg gtcacgacgt tactatggcg ctctgggtgc gagtggccga 2640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 2700

2641 ggtctaaata gtcgttattt ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt 2700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... AseI(2726) .......... .......... ..........

2701 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 2760

2701 tgaaataggc ggaggtaggt cagataatta acaacggccc ttcgatctca ttcatcaagc 2760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 2820

2761 ggtcaattat caaacgcgtt gcaacaacgg taacgatgtc cgtagcacca cagtgcgagc 2820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 2880

2821 agcaaaccat accgaagtaa gtcgaggcca agggttgcta gttccgctca atgtactagg 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 2940

2881 gggtacaaca cgttttttcg ccaatcgagg aagccaggag gctagcaaca gtcttcattc 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 3000

2941 aaccggcgtc acaatagtga gtaccaatac cgtcgtgacg tattaagaga atgacagtac 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 3060

3001 ggtaggcatt ctacgaaaag acactgacca ctcatgagtt ggttcagtaa gactcttatc 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 3120

3061 acatacgccg ctggctcaac gagaacgggc cgcagttatg ccctattatg gcgcggtgta 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 3180

3121 tcgtcttgaa attttcacga gtagtaacct tttgcaagaa gccccgcttt tgagagttcc 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 3240

3181 tagaatggcg acaactctag gtcaagctac attgggtgag cacgtgggtt gactagaagt 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 3300

3241 cgtagaaaat gaaagtggtc gcaaagaccc actcgttttt gtccttccgt tttacggcgt 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 3360

3301 tttttccctt attcccgctg tgcctttaca acttatgagt atgagaagga aaaagttata 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 3420

3361 ataacttcgt aaatagtccc aataacagag tactcgccta tgtataaact tacataaatc 3420

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 3480

3421 tttttatttg tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actgcgcggg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 3540

3481 acatcgccgc gtaattcgcg ccgcccacac caccaatgcg cgtcgcactg gcgatgtgaa 3540

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 3600

3541 cggtcgcggg atcgcgggcg aggaaagcga aagaagggaa ggaaagagcg gtgcaagcgg 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 3660

3601 ccgaaagggg cagttcgaga tttagccccc gagggaaatc ccaaggctaa atcacgaaat 3660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 3720

3661 gccgtggagc tggggttttt tgaactaatc ccactaccaa gtgcatcacc cggtagcggg 3720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3780

3721 actatctgcc aaaaagcggg aaactgcaac ctcaggtgca agaaattatc acctgagaac 3780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 3840

3781 aaggtttgac cttgttgtga gttgggatag agccagataa gaaaactaaa tattccctaa 3840

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3900

3841 aacggctaaa gccggataac caatttttta ctcgactaaa ttgtttttaa attgcgctta 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg caactgttgg 3960

3901 aaattgtttt ataattgcga atgttaaacg gtaagcggta agtccgacgc gttgacaacc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta 4020

3961 cttcccgcta gccacgcccg gagaagcgat aatgcggtcg ggttcgatgg tactattcat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agtaatatta aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat 4080

4021 tcattataat tccatgccct ccatgaacct cgccggcgtt attttataga aataaaagta 4080

.......... .......... .......... ........ synth_PA_term ........

.......... .......... .......... .......... .......... ..........

4081 tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa 4140

4081 atgtagacac acaaccaaaa aacacactta gctatcatga ttgtatgcga gaggtagttt 4140

........ synth_PA_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca 4200

4141 tgttttgctt tgttttgttt gatcgtttta tccgacaggg gtcacgttca cgtccacggt 4200

.......... .......... RVpri3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaacatttct ctatcgata. .......... .......... .......... .......... 4219

4201 cttgtaaaga gatagctat. .......... .......... .......... .......... 4219

.......... .......... .......... .......... .......... ..........