Sequence of pCCG

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Sequence of pCCG_C-3xFLAG

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Sequence of pCCG::C-3xFLAG: 8019 BP; 1985 A; 2098 C; 2064 G; 1872 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60

1 atatcacagt ggatttagca tacacatact atgtattcca atacataatt aacatcggcg 60

Sp6_primer .......... .......... .......... .......... ..........

.......... .......... NdeI(82) .......... .......... ..........

61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120

61 caagattgct gttatacagg tataccacgt gagagtcatg ttagacgaga ctacggcgta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180

121 tcaattcggt cggggctgtg ggcggttgtg ggcgactgcg cgggactgcc cgaacagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240

181 agggccgtag gcgaatgtct gttcgacact ggcagaggcc ctcgacgtac acagtctcca 240

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300

241 aaagtggcag tagtggcttt gcgcgctctg ctttcccgga gcactatgcg gataaaaata 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360

301 tccaattaca gtactattat taccaaagaa tctgcagtcc accgtgaaaa gcccctttac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420

361 acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata ggcgagtact 420

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480

421 ctgttattgg gactatttac gaagttatta taactttttc cttctcatac tcataagttg 480

.......... .......... .......... .......... ....... Ampicillin ORF 2

.......... .......... .......... .......... .......... ..........

481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540

481 taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg 540

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600

541 gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt 600

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660

601 agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag 660

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720

661 gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca taactgcggc 720

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ScaI(775)

721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780

721 ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg 780

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840

781 gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt 840

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900

841 attggtactc actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc 900

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960

901 tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg 960

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020

961 gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga catcgttacc 1020

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080

1021 gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta 1080

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140

1081 attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc 1140

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200

1141 gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac 1200

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260

1201 gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag 1260

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320

1261 tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg 1320

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380

1321 taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440

1381 aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500

1441 ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa 1500

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560

1501 ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620

1561 gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680

1621 cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740

1681 tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800

1741 ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860

1801 gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920

1861 tgtggcttga ctctatggat gtcgcactcg taactctttc gcggtgcgaa gggcttccct 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980

1921 ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040

1981 aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100

2041 tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160

2101 gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220

2161 ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280

2221 cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcgggttatg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340

2281 cgtttggcgg agaggggcgc gcaaccggct aagtaattac gtccaattgg accgaatagc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400

2341 tttaattatg ctgagtgata tccctctggc cgtctaggtt acgcctacct aagcgagtga 2400

......... T7_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460

2401 gccacggcgc cgccacgctt caaggacggc acctaacccc ctaacgtgtc tgccacctca 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520

2461 cgaacgatgt cgatccatgg gtcaacgtgt ggtttgccac ggggacacgg ggacgcagat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580

2521 ccacacagga cactcgttga cgcgggcgcg cgccgtggcc cttcgacgcc cttggagtta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640

2581 cctactaacc gcggtctagg ggccgtagtg gagtcgtgta cccgtcgggc tcttccttag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700

2641 ctcccatctt ctataaggtc ccaaaggcga tcgtgttgaa ggttcgcgga gatggcgatg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760

2701 aggtgtgtgg gtgcgactta ccgtgttggt gacatccgga tcgccagggt cgaggggcat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820

2761 tggtgcgtcg gggtcgctgg ctccagtagt actgtgatac tgctgttaga ccgccctctg 2820

.......... .......... .......... .......... .......... ..........

.......... ClaI(2831) .......... .......... .......... ..........

2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880

2821 aaccctttta gctactgccc gtggatggaa cccttgttat ctcgttcagg tcagttgcgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940

2881 gatcccttcc cccttttttt ttttctcggg taagagtgtt cctcgttgtg acacgacgat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000

2941 agtatcatgt gatgttcgtt tcagccctac aacgatctta ggcagccgat gcagccatct 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060

3001 atggagggta tccgtcattc agatccatag gttaaatatc aaggcgcata aagaagcagc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120

3061 ttgccccgtg tcgtttcgga ttgtcggcgt cacggagcgt tggttgtggt tcgtcattag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180

3121 agtaggtagg tatgtctagg agggtgttat agaaatgtac gcgtgaacgc gacacaatcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240

3181 tcaaatccaa ggtgatggtt ccgactgtgc tcgtcgtgac tgtcgtttga cgacgaaccc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300

3241 cggttcttta accgtcaaac gactcgtgga tgtatagtca tgtgtagttg ggaccaaatt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360

3301 aacaatcgga ccatgacaat tggcagtact tgctcctgca cctccttctt ggtgcgattg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420

3361 ttaacttggt cccaactaca tatggagaga atggtataaa cgaacgaagg tgcagggata 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480

3421 ggagttcgct aaaacaagaa ctgaaaaaca taaagttgcg tttccggtaa gtccaaagag 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540

3481 tacaagttgg gaaacctacc gctacggcaa gcagggtcct ggagcactgg aaactttctg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600

3541 ctctttatat agggtttcgt tgaagacgaa tacctgttcg ttgacgtacc gccgggtgtt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ScaI(3640) .......... ..........

3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660

3601 gagtatagag cgcattagta ggtttgcgat ccgggctcat gagatgacaa gagctctgtg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720

3661 gatggtatta tacatgggtt agtctgtact gttgtcagtt ggtttgtatg ggactaggcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780

3721 ggtttgggac tgtagggatc tactaccaat ccgtgtgttt accttttgcc ctgagcaacc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... XhoI(3833)

3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840

3781 aatccaccct tgcgaacata acgtcggtcg gttggctgag aggagcaggg agagctcagg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900

3841 gcaataacgg caaactgggt cggcgggtcg gcggtttgtc gactccctcg gttaccagat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960

3901 tatgtggcag gttgccgggg tcactacaaa gtttgaacta actgtcgctt gctttgggga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac 4020

3961 ctttgggcaa tatctataca tatctaaact gccccatcga accgggaatt atgctgagtg 4020

.......... .......... .......... .......... ......... T7_promoter

.......... SacI(4040) .......... .......... .......... ..........

4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg 4080

4021 atatcccgct taacctcgag gtggcgccac cgccggcgat cttcctcgtc aggtagacgc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag 4140

4081 acttagtgct ctcttagtcg atgaaactta gctacctacg tcgatggtct tcagtgagtc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc 4200

4141 aagcaagttt cggtgtagtg acccgtgaag gtaaccctgt ccgtaactag cctgctctgg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc 4260

4201 ctgaagaccg gcgaaagttg tcggtgtaat ataggtacag tgccgatgcg cagccggaag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt 4320

4261 ccattgtctt ttcgtgtgtc tgtcgctaac actgtaccta agcccgtttc gtaaccacca 4320

.......... .......... .......... .......... .......... ..........

SexAI(4325) ........ .......... .......... .......... ..........

4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag 4380

4321 gcgtggtcca gtaggactca cacgtcaccg acgataagtc taaagtagat tgacgccctc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt 4440

4381 tccccaagtt tccccgcact gcagtgtctg ttgcccactt cctgcttcta acggagtgaa 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca 4500

4441 gaaacgatcg ttaaacggac gtttcttccg cgtgtactgt tcgtttgttg accctttggt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg 4560

4501 gataacttat gggtgttacg tttcgagcct tccatgcaga actaacgtca cacagctcac 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt 4620

4561 agttttttct tcgttcacaa gtacgttcgg ttttaaccgt ggaggaggtg aagaggctca 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac 4680

4621 cggggtgggc ttggaggtcc gctctaccgg ccttgtatgg taggcgcaac cctaatactg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg 4740

4681 catagaggaa gaagaagtgt actaaggtag ggcaacaacg aacaaacgct cgacactgcc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ........ HindIII(4796)

4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt 4800

4741 ctctagcatc tacggtgaag cccggtccgt ccgtcacgtc cgtcggtcct tgtgttcgaa 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc 4860

4801 ggttgaacca gtagagctaa cggctaattc ccttggttta cggatatatt ctgacaggag 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct 4920

4861 ggtggagggg ttatggtaag aaaagaagaa ggtagtagtc ggttgtttcg ttagtgtaga 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa 4980

4921 agtgatgaag tttagttgtg ttgtgagttt ggtgaaagtg ttggggagtg tagttggttt 4980

.......... .......... .......... .......... .......... ..........

XbaI SpeI(4988) BamHI(4994),XmaI(4999),SmaI(5001) ....

4981 tctagaacta gtggatcccc gggttaatta aagactacaa agaccatgac ggtgattata 5040

4981 agatcttgat cacctagggg cccaattaat ttctgatgtt tctggtactg ccactaatat 5040

pBluescriptSK_primer .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aagatcatga catcgactac aaggatgacg atgacaagta gtgatcccgg gtggcatccc 5100

5041 ttctagtact gtagctgatg ttcctactgc tactgttcat cactagggcc caccgtaggg 5100

.......... .......... .......... EK .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc 5160

5101 acactgggga ggggtcacgg agaggaccgg gaccttcaac ggtgaggtca cgggtggtcg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt 5220

5161 gaacaggatt attttaattc aacgtagtaa aacagactga tccacaggaa gatattataa 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgaattcga 5280

5221 taccccacct ccccccacca tacctcgttc cccgttcaac ccttctgttg gacttaagct 5280

.......... .......... .......... .......... .......... ..........

EcoRV,HindIII ClaI SalI XhoI ApaI5320) KpnI(5326)

5281 tatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccagc ttttgttccc 5340

5281 atagttcgaa tagctatggc agctggagct cccccccggg ccatgggtcg aaaacaaggg 5340

.......... ..... pBluescriptKS_primer ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tttagtgagg gttaattagg gcgtggacgg ctaatggggt ctgaatgcta aaagacacca 5400

5341 aaatcactcc caattaatcc cgcacctgcc gattacccca gacttacgat tttctgtggt 5400

T3_promoter ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tttcccacac tccctctgat tttttttatg gttttttttt atcttcattt ttcgccgtct 5460

5401 aaagggtgtg agggagacta aaaaaaatac caaaaaaaaa tagaagtaaa aagcggcaga 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tttattcggt tgtttgggag gttcacatgc aacgtgcaag actgcaagtt ttttgagatg 5520

5461 aaataagcca acaaaccctc caagtgtacg ttgcacgttc tgacgttcaa aaaactctac 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ggcgcttcat gcaatgcagc atcactgcag ggacattgtg acatccacgt ttcgatcgtc 5580

5521 ccgcgaagta cgttacgtcg tagtgacgtc cctgtaacac tgtaggtgca aagctagcag 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgtgcttccc ccgttcatgt acgcatgatc tgaaaaaaaa aaaaaaaaaa aaggttgatg 5640

5581 acacgaaggg ggcaagtaca tgcgtactag actttttttt tttttttttt ttccaactac 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 taataatgaa aataaacacc cagggacgag cacctcatct gctggaatag ggagaaaaac 5700

5641 attattactt ttatttgtgg gtccctgctc gtggagtaga cgaccttatc cctctttttg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tcaagggccg tttgggtctc cacacttacc aacagattca tgtactgatc ttcagcctgt 5760

5701 agttcccggc aaacccagag gtgtgaatgg ttgtctaagt acatgactag aagtcggaca 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 acagaacagt acaatgtcca aaactcgtta tcattactct atgtgcccaa aatgacccag 5820

5761 tgtcttgtca tgttacaggt tttgagcaat agtaatgaga tacacgggtt ttactgggtc 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 atatacatca acccactaca tccttttgtt cttgtttttt tttttttttt ttgctacttc 5880

5821 tatatgtagt tgggtgatgt aggaaaacaa gaacaaaaaa aaaaaaaaaa aacgatgaag 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gaaaagaatt tccgtttagt tgctcttgct catgtgctca agacggatgc tgaccgccct 5940

5881 cttttcttaa aggcaaatca acgagaacga gtacacgagt tctgcctacg actggcggga 5940

.......... .......... .......... ORF 1 .......... ..........

.......... XhoI(5951) .......... .......... .......... ..........

5941 tctgtgagcc tcgagctcct caaccttagc caactgcatg acagcctggc cgacgttttt 6000

5941 agacactcgg agctcgagga gttggaatcg gttgacgtac tgtcggaccg gctgcaaaaa 6000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gagaccctcg gcagtcaagt tggagctggt aatgtgcttg acgaacgagg cgaaattgac 6060

6001 ctctgggagc cgtcagttca acctcgacca ttacacgaac tgcttgctcc gctttaactg 6060

.......... .......... ORF 1 .......... .......... ..........

ScaI(6069) .......... .......... .......... .......... ..........

6061 gccagagtac tgcttgccaa agccataggt agctatgtgt cttgacgtta gcagaagaat 6120

6061 cggtctcatg acgaacggtt tcggtatcca tcgatacaca gaactgcaat cgtcttctta 6120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ctgtgaagcg gggctattta tgatatgtaa cttacgcagc gagtggttaa caccagcaga 6180

6121 gacacttcgc cccgataaat actatacatt gaatgcgtcg ctcaccaatt gtggtcgtct 6180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gtaatcgcca acggactcag gagtccaggc gccaatgaag acactaccgg cgttcatgac 6240

6181 cattagcggt tgcctgagtc ctcaggtccg cggttacttc tgtgatggcc gcaagtactg 6240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aagatcgaca gccttctcgg cctccttgat ctggaggatc aagtgctcag gagcgtactt 6300

6241 ttctagctgt cggaagagcc ggaggaacta gacctcctag ttcacgagtc ctcgcatgaa 6300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gttgctgagc tccatggcct cctcgacggt cttgacctgc acggtgatcg agtgggcgat 6360

6301 caacgactcg aggtaccgga ggagctgcca gaactggacg tgccactagc tcacccgcta 6360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ggagccacgg acaatctgga cgcgaggaag ctccgtagcc tgacggtgaa cctcgtcctc 6420

6361 cctcggtgcc tgttagacct gcgctccttc gaggcatcgg actgccactt ggagcaggag 6420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 aatagcctga agatgctcct cgtcgaggtc aatagcgatc aggatgacct gactgtcaac 6480

6421 ttatcggact tctacgagga gcagctccag ttatcgctag tcctactgga ctgacagttg 6480

.......... .......... ORF 1 ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gccgtgctca gcctgggaca ggagatccga ggcaacgaac gcggggttgg cgtccttgtc 6540

6481 cggcacgagt cggaccctgt cctctaggct ccgttgcttg cgccccaacc gcaggaacag 6540

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 agcgatgacc agcacctcgg acgggccagc gggcatgtca ataccaacgg cagcgttggt 6600

6541 tcgctactgg tcgtggagcc tgcccggtcg cccgtacagt tatggttgcc gtcgcaacca 6600

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gtcgttgctg acgaacatct tggcagcagt gacgaactgg ttaccggggc cgagaatctt 6660

6601 cagcaacgac tgcttgtaga accgtcgtca ctgcttgacc aatggccccg gctcttagaa 6660

.......... ......... ORF 1 ORF 1 ......... .......... ..........

SalI(6662) .......... .......... NcoI(6694) .......... ..........

6661 gtcgaccttg gtgatgctct cggtgccgta ggccatggca gctacggcct gggcaccgcc 6720

6661 cagctggaac cactacgaga gccacggcat ccggtaccgt cgatgccgga cccgtggcgg 6720

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ggcaagcacg atggactcgg ccccaacctt gtgagcgacg tggacaatct cgggagtgat 6780

6721 ccgttcgtgc tacctgagcc ggggttggaa cactcgctgc acctgttaga gccctcacta 6780

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ggttccgtcg gcgcggggag gagaggcgaa cacaatcttg ttgcagccgg cgaccatggc 6840

6781 ccaaggcagc cgcgcccctc ctctccgctt gtgttagaac aacgtcggcc gctggtaccg 6840

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gggaacaccc agcataaggg cagtgctggg gagaacggcg gtaccgccgg ggatgtagca 6900

6841 cccttgtggg tcgtattccc gtcacgaccc ctcttgccgc catggcggcc cctacatcgt 6900

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gccgacggcc tcgatgggac gagagaaacg gctgcagaca acaccgggca tggtctcgac 6960

6901 cggctgccgg agctaccctg ctctctttgc cgacgtctgt tgtggcccgt accagagctg 6960

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ctggaggggc ttctcctcct tctgggcggc gtggaacttg cggatgttct cgaaggacac 7020

6961 gacctccccg aagaggagga agacccgccg caccttgaac gcctacaaga gcttcctgtg 7020

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gtcgatggca gcaatggtct cctcagggag ctgcataagc tccttgggga agggcgcctt 7080

7021 cagctaccgt cgttaccaga ggagtccctc gacgtattcg aggaacccct tcccgcggaa 7080

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 caggacgggg ctagtaagag aggtagcctt ctcgaacttg tgagtgtacg acagaacagc 7140

7081 gtcctgcccc gatcattctc tccatcggaa gagcttgaac actcacatgc tgtcttgtcg 7140

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cttgtcgccg ttcttgcgga cgtcctcgat gatggggaca atgatcttgt agatggcatc 7200

7141 gaacagcggc aagaacgcct gcaggagcta ctacccctgt tactagaaca tctaccgtag 7200

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... SacI(7242) ..........

7201 ggacgacttt tgcgcaggac gcttgagagc agcatcgagc tcctcggtag agaccttgga 7260

7201 cctgctgaaa acgcgtcctg cgaactctcg tcgtagctcg aggagccatc tctggaacct 7260

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ggcgtcgaaa cgtctcatgg tgatcttctc aggggtggtc tcctgggcgg cctccttggt 7320

7261 ccgcagcttt gcagagtacc actagaagag tccccaccag aggacccgcc ggaggaacca 7320

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gacaggggcc gaagtggcag cgagagcgga cgccgcaggc ttgatgccct ccttctcagc 7380

7321 ctgtccccgg cttcaccgtc gctctcgcct gcggcgtccg aactacggga ggaagagtcg 7380

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ccacttaccc ttagcatctc cagtcctgcg cttgaccttc cagctcttgg cgtcaaggct 7440

7381 ggtgaatggg aatcgtagag gtcaggacgc gaactggaag gtcgagaacc gcagttccga 7440

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cctttcgata tcggcaagag taacgccggc agcaacggcc ctggtaagag caaagtagaa 7500

7441 ggaaagctat agccgttctc attgcggccg tcgttgccgg gaccattctc gtttcatctt 7500

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 gagatcggca gcctcaaagg cgatttcctg gggggtctga gcggtgcaga gctcctcagc 7560

7501 ctctagccgt cggagtttcc gctaaaggac cccccagact cgccacgtct cgaggagtcg 7560

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ctcctccatg atcttggccc ggactagctt ctcatcggag aagagacggg cagtgtagga 7620

7561 gaggaggtac tagaaccggg cctgatcgaa gagtagcctc ttctctgccc gtcacatcct 7620

ORF 1 ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gccctcgggg gcagactgtt tcctcgaaat caaagtctgc tcgagcttgg gaaggccttt 7680

7621 cgggagcccc cgtctgacaa aggagcttta gtttcagacg agctcgaacc cttccggaaa 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gagctgacca aagcagccgg actgatcgag gtggcagaaa cgacccttct gcttcacgac 7740

7681 ctcgactggt ttcgtcggcc tgactagctc caccgtcttt gctgggaaga cgaagtgctg 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 aaacttgaga gcatcgttat cgcagtcaag cgagatgcgg acgagctcct gagtgtctcc 7800

7741 tttgaactct cgtagcaata gcgtcagttc gctctacgcc tgctcgagga ctcacagagg 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ggaagtagca cccttgtacc agagaccgcg cttccggctc tgatagacac cagtctgtgt 7860

7801 ccttcatcgt gggaacatgg tctctggcgc gaaggccgag actatctgtg gtcagacaca 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 cctgagggcc tcgttcacac tctcggcact gctgtagacc agacccagag cagtgtcgtg 7920

7861 ggactcccgg agcaagtgtg agagccgtga cgacatctgg tctgggtctc gtcacagcac 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... XhoI(7977)

7921 ctcatcaacg acaacggtgg ggagaagacc atcgggacgg tcagacttcc agacgctcga 7980

7921 gagtagttgc tgttgccacc cctcttctgg tagccctgcc agtctgaagg tctgcgagct 7980

.......... .......... .......... .......... .......... ..........

........ HindIII(8000) .. PvuII(8008),XhoI(8012) .... ..........

7981 gaggatggtg gagatggcaa gcttcagctg ctcgagttc. .......... .......... 8019

7981 ctcctaccac ctctaccgtt cgaagtcgac gagctcaag. .......... .......... 8019

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-8019 Reference author: Honda S., Selker E.U.; Reference title: "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene Reference title: replacement, protein expression and protein purification"; Reference location: Genetics 0:0-0(2009).
Reference number: [2] Reference positions: 1-8019 Reference author: Honda S., Selker E.U.; Reference title: ; Reference location: Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA