_V_VT7ID FJ457002; SV 1; circular; other DNA; STD; SYN; 8509 BP. AC FJ457002; DT 30-JAN-2009 (Rel. 99, Created) DT 30-JAN-2009 (Rel. 99, Last updated, Version 1) DE Neurospora expression vector pCCG::C-Gly::GFP, complete sequence. KW . OS Neurospora expression vector pCCG::C-Gly::GFP OC other sequences; artificial sequences; vectors. RN [1] RP 1-8509 RA Honda S., Selker E.U.; RT "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene RT replacement, protein expression and protein purification"; RL Genetics 0:0-0(2009). RN [2] RP 1-8509 RA Honda S., Selker E.U.; RT ; RL Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. RL Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA FH Key Location/Qualifiers FT source 1..8509 FT /organism="Neurospora expression vector pCCG::C-Gly::GFP" FT /mol_type="other DNA" FT /db_xref="taxon:591005" FT promoter complement(1..17) FT /note="SP6" FT CDS 468..1328 FT /codon_start=1 FT /transl_table=11 FT /product="beta-lactamase" FT /note="ampicillin resistance" FT /protein_id="ACM10002.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT promoter complement(2345..2365) FT /note="T7" FT misc_feature complement(2374..4006) FT /note="3' flank region of Neurospora crassa his-3 gene" FT promoter 4057..4985 FT /note="Neurospora crassa ccg-1 (grg-1)" FT misc_feature 5013..5042 FT /note="10xGly" FT CDS 5043..5762 FT /codon_start=1 FT /transl_table=11 FT /product="GFP" FT /protein_id="ACM10003.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYK" FT misc_feature 5763..5847 FT /note="T3 promoter and multiple cloning site from FT pBluescript KS+" FT misc_feature 6583..8490 FT /note="5' truncated histidine biosynthesis trifunctional FT protein; includes phosphoribosyl-AMP cyclohydrolase, FT phosphoribosyl-ATP cyclohydrolase and histindinol FT dehydrogenase" SQ Sequence 8509 BP; 2095 A; 2283 C; 2218 G; 1913 T; 0 other; tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac 4020 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg 4080 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag 4140 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc 4200 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc 4260 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt 4320 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag 4380 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt 4440 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca 4500 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg 4560 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt 4620 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac 4680 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg 4740 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt 4800 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc 4860 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct 4920 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa 4980 tctagaacta gtggatcccc gggttaatta agggcggagg cggcggaggc ggaggcggag 5040 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 5100 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 5160 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 5220 ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 5280 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 5340 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 5400 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 5460 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 5520 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 5580 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 5640 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 5700 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 5760 aagaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccagc 5820 ttttgttccc tttagtgagg gttaattagg gcgtggacgg ctaatggggt ctgaatgcta 5880 aaagacacca tttcccacac tccctctgat tttttttatg gttttttttt atcttcattt 5940 ttcgccgtct tttattcggt tgtttgggag gttcacatgc aacgtgcaag actgcaagtt 6000 ttttgagatg ggcgcttcat gcaatgcagc atcactgcag ggacattgtg acatccacgt 6060 ttcgatcgtc tgtgcttccc ccgttcatgt acgcatgatc tgaaaaaaaa aaaaaaaaaa 6120 aaggttgatg taataatgaa aataaacacc cagggacgag cacctcatct gctggaatag 6180 ggagaaaaac tcaagggccg tttgggtctc cacacttacc aacagattca tgtactgatc 6240 ttcagcctgt acagaacagt acaatgtcca aaactcgtta tcattactct atgtgcccaa 6300 aatgacccag atatacatca acccactaca tccttttgtt cttgtttttt tttttttttt 6360 ttgctacttc gaaaagaatt tccgtttagt tgctcttgct catgtgctca agacggatgc 6420 tgaccgccct tctgtgagcc tcgagctcct caaccttagc caactgcatg acagcctggc 6480 cgacgttttt gagaccctcg gcagtcaagt tggagctggt aatgtgcttg acgaacgagg 6540 cgaaattgac gccagagtac tgcttgccaa agccataggt agctatgtgt cttgacgtta 6600 gcagaagaat ctgtgaagcg gggctattta tgatatgtaa cttacgcagc gagtggttaa 6660 caccagcaga gtaatcgcca acggactcag gagtccaggc gccaatgaag acactaccgg 6720 cgttcatgac aagatcgaca gccttctcgg cctccttgat ctggaggatc aagtgctcag 6780 gagcgtactt gttgctgagc tccatggcct cctcgacggt cttgacctgc acggtgatcg 6840 agtgggcgat ggagccacgg acaatctgga cgcgaggaag ctccgtagcc tgacggtgaa 6900 cctcgtcctc aatagcctga agatgctcct cgtcgaggtc aatagcgatc aggatgacct 6960 gactgtcaac gccgtgctca gcctgggaca ggagatccga ggcaacgaac gcggggttgg 7020 cgtccttgtc agcgatgacc agcacctcgg acgggccagc gggcatgtca ataccaacgg 7080 cagcgttggt gtcgttgctg acgaacatct tggcagcagt gacgaactgg ttaccggggc 7140 cgagaatctt gtcgaccttg gtgatgctct cggtgccgta ggccatggca gctacggcct 7200 gggcaccgcc ggcaagcacg atggactcgg ccccaacctt gtgagcgacg tggacaatct 7260 cgggagtgat ggttccgtcg gcgcggggag gagaggcgaa cacaatcttg ttgcagccgg 7320 cgaccatggc gggaacaccc agcataaggg cagtgctggg gagaacggcg gtaccgccgg 7380 ggatgtagca gccgacggcc tcgatgggac gagagaaacg gctgcagaca acaccgggca 7440 tggtctcgac ctggaggggc ttctcctcct tctgggcggc gtggaacttg cggatgttct 7500 cgaaggacac gtcgatggca gcaatggtct cctcagggag ctgcataagc tccttgggga 7560 agggcgcctt caggacgggg ctagtaagag aggtagcctt ctcgaacttg tgagtgtacg 7620 acagaacagc cttgtcgccg ttcttgcgga cgtcctcgat gatggggaca atgatcttgt 7680 agatggcatc ggacgacttt tgcgcaggac gcttgagagc agcatcgagc tcctcggtag 7740 agaccttgga ggcgtcgaaa cgtctcatgg tgatcttctc aggggtggtc tcctgggcgg 7800 cctccttggt gacaggggcc gaagtggcag cgagagcgga cgccgcaggc ttgatgccct 7860 ccttctcagc ccacttaccc ttagcatctc cagtcctgcg cttgaccttc cagctcttgg 7920 cgtcaaggct cctttcgata tcggcaagag taacgccggc agcaacggcc ctggtaagag 7980 caaagtagaa gagatcggca gcctcaaagg cgatttcctg gggggtctga gcggtgcaga 8040 gctcctcagc ctcctccatg atcttggccc ggactagctt ctcatcggag aagagacggg 8100 cagtgtagga gccctcgggg gcagactgtt tcctcgaaat caaagtctgc tcgagcttgg 8160 gaaggccttt gagctgacca aagcagccgg actgatcgag gtggcagaaa cgacccttct 8220 gcttcacgac aaacttgaga gcatcgttat cgcagtcaag cgagatgcgg acgagctcct 8280 gagtgtctcc ggaagtagca cccttgtacc agagaccgcg cttccggctc tgatagacac 8340 cagtctgtgt cctgagggcc tcgttcacac tctcggcact gctgtagacc agacccagag 8400 cagtgtcgtg ctcatcaacg acaacggtgg ggagaagacc atcgggacgg tcagacttcc 8460 agacgctcga gaggatggtg gagatggcaa gcttcagctg ctcgagttc 8509 =!pCCG::C-Gly::GFP"Times New Roman _VNdeI ArialR=!vpjv _VScaI Arial=!+ _V+HpaI(2327),KpnI(2480),NheI(2669),ClaI(2831) Arial =!Xajj _V7HpaI(3320),ScaI(3640),XhoI(3652),XhoI(3833),PvuII(3881) ArialD=!>GOO _VMSacI(4040),EagI(4053),NotI(4053),ClaI(4111),MluI(4248),SexAI(4325),NheI(4446) Arial=!T^gjg _VPHindIII(4796),XbaI(4982),SpeI(4988),BamHI(4994),XmaI(4999),SmaI(5001),PacI(5009) Arial=!5=EE _VgEcoRI(5764),EcoRV(5772),HindIII(5776),ClaI(5783),SalI(5791),XhoI(5797),ApaI(5810),KpnI(5816),PstI(6040) Arial=!U _VAXhoI(6441),SacI(6448),ScaI(6559),HpaI(6659),SacI(6802),NcoI(6803) Arial=!SRPI,t _VCSalI(7152),NcoI(7184),NcoI(7325),KpnI(7375),PstI(7427),Bsu36I(7533) Arial=!v _V7SacI(7732),EcoRV(7940),SacI(8044),XhoI(8151),SacI(8278) Ariall=!*&I _V