Sequence of pCCG_C-Gly

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Sequence of pCCG_C-Gly_GFP

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Sequence of pCCG::C-Gly::GFP: 8509 BP; 2095 A; 2283 C; 2218 G; 1913 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60

1 atatcacagt ggatttagca tacacatact atgtattcca atacataatt aacatcggcg 60

Sp6_primer .......... .......... .......... .......... ..........

.......... .......... NdeI(82) .......... .......... ..........

61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120

61 caagattgct gttatacagg tataccacgt gagagtcatg ttagacgaga ctacggcgta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180

121 tcaattcggt cggggctgtg ggcggttgtg ggcgactgcg cgggactgcc cgaacagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240

181 agggccgtag gcgaatgtct gttcgacact ggcagaggcc ctcgacgtac acagtctcca 240

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300

241 aaagtggcag tagtggcttt gcgcgctctg ctttcccgga gcactatgcg gataaaaata 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360

301 tccaattaca gtactattat taccaaagaa tctgcagtcc accgtgaaaa gcccctttac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420

361 acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata ggcgagtact 420

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480

421 ctgttattgg gactatttac gaagttatta taactttttc cttctcatac tcataagttg 480

.......... .......... .......... .......... ....... Ampicillin ORF 2

.......... .......... .......... .......... .......... ..........

481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540

481 taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg 540

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600

541 gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt 600

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660

601 agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag 660

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720

661 gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca taactgcggc 720

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ScaI(775)

721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780

721 ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg 780

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840

781 gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt 840

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900

841 attggtactc actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc 900

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960

901 tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg 960

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020

961 gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga catcgttacc 1020

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080

1021 gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta 1080

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140

1081 attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc 1140

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200

1141 gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac 1200

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260

1201 gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag 1260

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320

1261 tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg 1320

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380

1321 taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440

1381 aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500

1441 ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa 1500

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560

1501 ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620

1561 gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680

1621 cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740

1681 tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800

1741 ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860

1801 gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920

1861 tgtggcttga ctctatggat gtcgcactcg taactctttc gcggtgcgaa gggcttccct 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980

1921 ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040

1981 aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100

2041 tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160

2101 gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220

2161 ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280

2221 cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcgggttatg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340

2281 cgtttggcgg agaggggcgc gcaaccggct aagtaattac gtccaattgg accgaatagc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400

2341 tttaattatg ctgagtgata tccctctggc cgtctaggtt acgcctacct aagcgagtga 2400

......... T7_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460

2401 gccacggcgc cgccacgctt caaggacggc acctaacccc ctaacgtgtc tgccacctca 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520

2461 cgaacgatgt cgatccatgg gtcaacgtgt ggtttgccac ggggacacgg ggacgcagat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580

2521 ccacacagga cactcgttga cgcgggcgcg cgccgtggcc cttcgacgcc cttggagtta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640

2581 cctactaacc gcggtctagg ggccgtagtg gagtcgtgta cccgtcgggc tcttccttag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700

2641 ctcccatctt ctataaggtc ccaaaggcga tcgtgttgaa ggttcgcgga gatggcgatg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760

2701 aggtgtgtgg gtgcgactta ccgtgttggt gacatccgga tcgccagggt cgaggggcat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820

2761 tggtgcgtcg gggtcgctgg ctccagtagt actgtgatac tgctgttaga ccgccctctg 2820

.......... .......... .......... .......... .......... ..........

.......... ClaI(2831) .......... .......... .......... ..........

2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880

2821 aaccctttta gctactgccc gtggatggaa cccttgttat ctcgttcagg tcagttgcgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940

2881 gatcccttcc cccttttttt ttttctcggg taagagtgtt cctcgttgtg acacgacgat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000

2941 agtatcatgt gatgttcgtt tcagccctac aacgatctta ggcagccgat gcagccatct 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060

3001 atggagggta tccgtcattc agatccatag gttaaatatc aaggcgcata aagaagcagc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120

3061 ttgccccgtg tcgtttcgga ttgtcggcgt cacggagcgt tggttgtggt tcgtcattag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180

3121 agtaggtagg tatgtctagg agggtgttat agaaatgtac gcgtgaacgc gacacaatcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240

3181 tcaaatccaa ggtgatggtt ccgactgtgc tcgtcgtgac tgtcgtttga cgacgaaccc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300

3241 cggttcttta accgtcaaac gactcgtgga tgtatagtca tgtgtagttg ggaccaaatt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360

3301 aacaatcgga ccatgacaat tggcagtact tgctcctgca cctccttctt ggtgcgattg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420

3361 ttaacttggt cccaactaca tatggagaga atggtataaa cgaacgaagg tgcagggata 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480

3421 ggagttcgct aaaacaagaa ctgaaaaaca taaagttgcg tttccggtaa gtccaaagag 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540

3481 tacaagttgg gaaacctacc gctacggcaa gcagggtcct ggagcactgg aaactttctg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600

3541 ctctttatat agggtttcgt tgaagacgaa tacctgttcg ttgacgtacc gccgggtgtt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ScaI(3640) .......... ..........

3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660

3601 gagtatagag cgcattagta ggtttgcgat ccgggctcat gagatgacaa gagctctgtg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720

3661 gatggtatta tacatgggtt agtctgtact gttgtcagtt ggtttgtatg ggactaggcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780

3721 ggtttgggac tgtagggatc tactaccaat ccgtgtgttt accttttgcc ctgagcaacc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840

3781 aatccaccct tgcgaacata acgtcggtcg gttggctgag aggagcaggg agagctcagg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... PvuII(3881) .........

3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900

3841 gcaataacgg caaactgggt cggcgggtcg gcggtttgtc gactccctcg gttaccagat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960

3901 tatgtggcag gttgccgggg tcactacaaa gtttgaacta actgtcgctt gctttgggga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac 4020

3961 ctttgggcaa tatctataca tatctaaact gccccatcga accgggaatt atgctgagtg 4020

.......... .......... .......... .......... ......... T7_promoter

.......... SacI(4040) .......... .......... .......... ..........

4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg 4080

4021 atatcccgct taacctcgag gtggcgccac cgccggcgat cttcctcgtc aggtagacgc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag 4140

4081 acttagtgct ctcttagtcg atgaaactta gctacctacg tcgatggtct tcagtgagtc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc 4200

4141 aagcaagttt cggtgtagtg acccgtgaag gtaaccctgt ccgtaactag cctgctctgg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc 4260

4201 ctgaagaccg gcgaaagttg tcggtgtaat ataggtacag tgccgatgcg cagccggaag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt 4320

4261 ccattgtctt ttcgtgtgtc tgtcgctaac actgtaccta agcccgtttc gtaaccacca 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag 4380

4321 gcgtggtcca gtaggactca cacgtcaccg acgataagtc taaagtagat tgacgccctc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt 4440

4381 tccccaagtt tccccgcact gcagtgtctg ttgcccactt cctgcttcta acggagtgaa 4440

.......... .......... .......... .......... .......... ..........

NheI(4446) .......... .......... .......... .......... ..........

4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca 4500

4441 gaaacgatcg ttaaacggac gtttcttccg cgtgtactgt tcgtttgttg accctttggt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg 4560

4501 gataacttat gggtgttacg tttcgagcct tccatgcaga actaacgtca cacagctcac 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt 4620

4561 agttttttct tcgttcacaa gtacgttcgg ttttaaccgt ggaggaggtg aagaggctca 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac 4680

4621 cggggtgggc ttggaggtcc gctctaccgg ccttgtatgg taggcgcaac cctaatactg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg 4740

4681 catagaggaa gaagaagtgt actaaggtag ggcaacaacg aacaaacgct cgacactgcc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt 4800

4741 ctctagcatc tacggtgaag cccggtccgt ccgtcacgtc cgtcggtcct tgtgttcgaa 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc 4860

4801 ggttgaacca gtagagctaa cggctaattc ccttggttta cggatatatt ctgacaggag 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct 4920

4861 ggtggagggg ttatggtaag aaaagaagaa ggtagtagtc ggttgtttcg ttagtgtaga 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa 4980

4921 agtgatgaag tttagttgtg ttgtgagttt ggtgaaagtg ttggggagtg tagttggttt 4980

.......... .......... .......... .......... .......... ..........

XbaI SpeI BamHI),XmaI(4999),SmaI(5001) PacI(5009)

4981 tctagaacta gtggatcccc gggttaatta agggcggagg cggcggaggc ggaggcggag 5040

4981 agatcttgat cacctagggg cccaattaat tcccgcctcc gccgcctccg cctccgcctc 5040

pBluescriptSK_primer .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 5100

5041 cgtaccactc gttcccgctc ctcgacaagt ggccccacca cgggtaggac cagctcgacc 5100

.......... .......... .......... ORF 3 ........ EGFP_N_primer

.......... .......... .......... .......... .......... ..........

5101 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 5160

5101 tgccgctgca tttgccggtg ttcaagtcgc acaggccgct cccgctcccg ctacggtgga 5160

EGFP_N_primer ...... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 5220

5161 tgccgttcga ctgggacttc aagtagacgt ggtggccgtt cgacgggcac gggaccgggt 5220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 5280

5221 gggagcactg gtggaagtgg atgccgcacg tcacgaagtc ggcgatgggg ctggtgtact 5280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 5340

5281 tcgtcgtgct gaagaagttc aggcggtacg ggcttccgat gcaggtcctc gcgtggtaga 5340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 5400

5341 agaagttcct gctgccgttg atgttctggg cgcggctcca cttcaagctc ccgctgtggg 5400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 5460

5401 accacttggc gtagctcgac ttcccgtagc tgaagttcct cctgccgttg taggaccccg 5460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 5520

5461 tgttcgacct catgttgatg ttgtcggtgt tgcagatata gtaccggctg ttcgtcttct 5520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 5580

5521 tgccgtagtt ccacttgaag ttctaggcgg tgttgtagct cctgccgtcg cacgtcgagc 5580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 5640

5581 ggctggtgat ggtcgtcttg tgggggtagc cgctgccggg gcacgacgac gggctgttgg 5640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 5700

5641 tgatggactc gtgggtcagg cgggactcgt ttctggggtt gctcttcgcg ctagtgtacc 5700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 5760

5701 aggacgacct caagcactgg cggcggccct agtgagagcc gtacctgctc gacatgttca 5760

EGFP_C_primer ...... ORF 3 .......... .......... ..........

EcoRI EcoRV,HindIII ClaI SalI XhoI797) ApaI(5810)

5761 aagaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccagc 5820

5761 ttcttaagct atagttcgaa tagctatggc agctggagct cccccccggg ccatgggtcg 5820

.......... .......... ..... pBluescriptKS_primer ..... ..........

.......... .......... .......... .......... .......... ..........

5821 ttttgttccc tttagtgagg gttaattagg gcgtggacgg ctaatggggt ctgaatgcta 5880

5821 aaaacaaggg aaatcactcc caattaatcc cgcacctgcc gattacccca gacttacgat 5880

......... T3_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aaagacacca tttcccacac tccctctgat tttttttatg gttttttttt atcttcattt 5940

5881 tttctgtggt aaagggtgtg agggagacta aaaaaaatac caaaaaaaaa tagaagtaaa 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ttcgccgtct tttattcggt tgtttgggag gttcacatgc aacgtgcaag actgcaagtt 6000

5941 aagcggcaga aaataagcca acaaaccctc caagtgtacg ttgcacgttc tgacgttcaa 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... PstI(6040) .......... ..........

6001 ttttgagatg ggcgcttcat gcaatgcagc atcactgcag ggacattgtg acatccacgt 6060

6001 aaaactctac ccgcgaagta cgttacgtcg tagtgacgtc cctgtaacac tgtaggtgca 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ttcgatcgtc tgtgcttccc ccgttcatgt acgcatgatc tgaaaaaaaa aaaaaaaaaa 6120

6061 aagctagcag acacgaaggg ggcaagtaca tgcgtactag actttttttt tttttttttt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 aaggttgatg taataatgaa aataaacacc cagggacgag cacctcatct gctggaatag 6180

6121 ttccaactac attattactt ttatttgtgg gtccctgctc gtggagtaga cgaccttatc 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ggagaaaaac tcaagggccg tttgggtctc cacacttacc aacagattca tgtactgatc 6240

6181 cctctttttg agttcccggc aaacccagag gtgtgaatgg ttgtctaagt acatgactag 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ttcagcctgt acagaacagt acaatgtcca aaactcgtta tcattactct atgtgcccaa 6300

6241 aagtcggaca tgtcttgtca tgttacaggt tttgagcaat agtaatgaga tacacgggtt 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aatgacccag atatacatca acccactaca tccttttgtt cttgtttttt tttttttttt 6360

6301 ttactgggtc tatatgtagt tgggtgatgt aggaaaacaa gaacaaaaaa aaaaaaaaaa 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttgctacttc gaaaagaatt tccgtttagt tgctcttgct catgtgctca agacggatgc 6420

6361 aacgatgaag cttttcttaa aggcaaatca acgagaacga gtacacgagt tctgcctacg 6420

.......... .......... .......... .......... ORF 1 ..........

.......... .......... XhoI(6441) .......... .......... ..........

6421 tgaccgccct tctgtgagcc tcgagctcct caaccttagc caactgcatg acagcctggc 6480

6421 actggcggga agacactcgg agctcgagga gttggaatcg gttgacgtac tgtcggaccg 6480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cgacgttttt gagaccctcg gcagtcaagt tggagctggt aatgtgcttg acgaacgagg 6540

6481 gctgcaaaaa ctctgggagc cgtcagttca acctcgacca ttacacgaac tgcttgctcc 6540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cgaaattgac gccagagtac tgcttgccaa agccataggt agctatgtgt cttgacgtta 6600

6541 gctttaactg cggtctcatg acgaacggtt tcggtatcca tcgatacaca gaactgcaat 6600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gcagaagaat ctgtgaagcg gggctattta tgatatgtaa cttacgcagc gagtggttaa 6660

6601 cgtcttctta gacacttcgc cccgataaat actatacatt gaatgcgtcg ctcaccaatt 6660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 caccagcaga gtaatcgcca acggactcag gagtccaggc gccaatgaag acactaccgg 6720

6661 gtggtcgtct cattagcggt tgcctgagtc ctcaggtccg cggttacttc tgtgatggcc 6720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cgttcatgac aagatcgaca gccttctcgg cctccttgat ctggaggatc aagtgctcag 6780

6721 gcaagtactg ttctagctgt cggaagagcc ggaggaacta gacctcctag ttcacgagtc 6780

.......... .......... ORF 1 .......... .......... ..........

.......... .... SacI(6802),NcoI(6803) .... .......... ..........

6781 gagcgtactt gttgctgagc tccatggcct cctcgacggt cttgacctgc acggtgatcg 6840

6781 ctcgcatgaa caacgactcg aggtaccgga ggagctgcca gaactggacg tgccactagc 6840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 agtgggcgat ggagccacgg acaatctgga cgcgaggaag ctccgtagcc tgacggtgaa 6900

6841 tcacccgcta cctcggtgcc tgttagacct gcgctccttc gaggcatcgg actgccactt 6900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cctcgtcctc aatagcctga agatgctcct cgtcgaggtc aatagcgatc aggatgacct 6960

6901 ggagcaggag ttatcggact tctacgagga gcagctccag ttatcgctag tcctactgga 6960

.......... .......... ORF 1 .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

6961 gactgtcaac gccgtgctca gcctgggaca ggagatccga ggcaacgaac gcggggttgg 7020

6961 ctgacagttg cggcacgagt cggaccctgt cctctaggct ccgttgcttg cgccccaacc 7020

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cgtccttgtc agcgatgacc agcacctcgg acgggccagc gggcatgtca ataccaacgg 7080

7021 gcaggaacag tcgctactgg tcgtggagcc tgcccggtcg cccgtacagt tatggttgcc 7080

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cagcgttggt gtcgttgctg acgaacatct tggcagcagt gacgaactgg ttaccggggc 7140

7081 gtcgcaacca cagcaacgac tgcttgtaga accgtcgtca ctgcttgacc aatggccccg 7140

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... SalI(7152) .......... .......... .......... ..........

7141 cgagaatctt gtcgaccttg gtgatgctct cggtgccgta ggccatggca gctacggcct 7200

7141 gctcttagaa cagctggaac cactacgaga gccacggcat ccggtaccgt cgatgccgga 7200

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gggcaccgcc ggcaagcacg atggactcgg ccccaacctt gtgagcgacg tggacaatct 7260

7201 cccgtggcgg ccgttcgtgc tacctgagcc ggggttggaa cactcgctgc acctgttaga 7260

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 cgggagtgat ggttccgtcg gcgcggggag gagaggcgaa cacaatcttg ttgcagccgg 7320

7261 gccctcacta ccaaggcagc cgcgcccctc ctctccgctt gtgttagaac aacgtcggcc 7320

.......... ......... ORF 1 ORF 1 ......... .......... ..........

NcoI(7325) .......... .......... .......... .......... ..........

7321 cgaccatggc gggaacaccc agcataaggg cagtgctggg gagaacggcg gtaccgccgg 7380

7321 gctggtaccg cccttgtggg tcgtattccc gtcacgaccc ctcttgccgc catggcggcc 7380

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ggatgtagca gccgacggcc tcgatgggac gagagaaacg gctgcagaca acaccgggca 7440

7381 cctacatcgt cggctgccgg agctaccctg ctctctttgc cgacgtctgt tgtggcccgt 7440

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tggtctcgac ctggaggggc ttctcctcct tctgggcggc gtggaacttg cggatgttct 7500

7441 accagagctg gacctccccg aagaggagga agacccgccg caccttgaac gcctacaaga 7500

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... ......... Bsu36I(7533) ......... ..........

7501 cgaaggacac gtcgatggca gcaatggtct cctcagggag ctgcataagc tccttgggga 7560

7501 gcttcctgtg cagctaccgt cgttaccaga ggagtccctc gacgtattcg aggaacccct 7560

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 agggcgcctt caggacgggg ctagtaagag aggtagcctt ctcgaacttg tgagtgtacg 7620

7561 tcccgcggaa gtcctgcccc gatcattctc tccatcggaa gagcttgaac actcacatgc 7620

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 acagaacagc cttgtcgccg ttcttgcgga cgtcctcgat gatggggaca atgatcttgt 7680

7621 tgtcttgtcg gaacagcggc aagaacgcct gcaggagcta ctacccctgt tactagaaca 7680

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 agatggcatc ggacgacttt tgcgcaggac gcttgagagc agcatcgagc tcctcggtag 7740

7681 tctaccgtag cctgctgaaa acgcgtcctg cgaactctcg tcgtagctcg aggagccatc 7740

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 agaccttgga ggcgtcgaaa cgtctcatgg tgatcttctc aggggtggtc tcctgggcgg 7800

7741 tctggaacct ccgcagcttt gcagagtacc actagaagag tccccaccag aggacccgcc 7800

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 cctccttggt gacaggggcc gaagtggcag cgagagcgga cgccgcaggc ttgatgccct 7860

7801 ggaggaacca ctgtccccgg cttcaccgtc gctctcgcct gcggcgtccg aactacggga 7860

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ccttctcagc ccacttaccc ttagcatctc cagtcctgcg cttgaccttc cagctcttgg 7920

7861 ggaagagtcg ggtgaatggg aatcgtagag gtcaggacgc gaactggaag gtcgagaacc 7920

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 cgtcaaggct cctttcgata tcggcaagag taacgccggc agcaacggcc ctggtaagag 7980

7921 gcagttccga ggaaagctat agccgttctc attgcggccg tcgttgccgg gaccattctc 7980

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 caaagtagaa gagatcggca gcctcaaagg cgatttcctg gggggtctga gcggtgcaga 8040

7981 gtttcatctt ctctagccgt cggagtttcc gctaaaggac cccccagact cgccacgtct 8040

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gctcctcagc ctcctccatg atcttggccc ggactagctt ctcatcggag aagagacggg 8100

8041 cgaggagtcg gaggaggtac tagaaccggg cctgatcgaa gagtagcctc ttctctgccc 8100

ORF 1 ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 cagtgtagga gccctcgggg gcagactgtt tcctcgaaat caaagtctgc tcgagcttgg 8160

8101 gtcacatcct cgggagcccc cgtctgacaa aggagcttta gtttcagacg agctcgaacc 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 gaaggccttt gagctgacca aagcagccgg actgatcgag gtggcagaaa cgacccttct 8220

8161 cttccggaaa ctcgactggt ttcgtcggcc tgactagctc caccgtcttt gctgggaaga 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SacI(8278)

8221 gcttcacgac aaacttgaga gcatcgttat cgcagtcaag cgagatgcgg acgagctcct 8280

8221 cgaagtgctg tttgaactct cgtagcaata gcgtcagttc gctctacgcc tgctcgagga 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 gagtgtctcc ggaagtagca cccttgtacc agagaccgcg cttccggctc tgatagacac 8340

8281 ctcacagagg ccttcatcgt gggaacatgg tctctggcgc gaaggccgag actatctgtg 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 cagtctgtgt cctgagggcc tcgttcacac tctcggcact gctgtagacc agacccagag 8400

8341 gtcagacaca ggactcccgg agcaagtgtg agagccgtga cgacatctgg tctgggtctc 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 cagtgtcgtg ctcatcaacg acaacggtgg ggagaagacc atcgggacgg tcagacttcc 8460

8401 gtcacagcac gagtagttgc tgttgccacc cctcttctgg tagccctgcc agtctgaagg 8460

.......... .......... .......... .......... .......... ..........

XhoI(8467) ........ HindIII(8490) .. PvuII(8498),XhoI(8502) ....

8461 agacgctcga gaggatggtg gagatggcaa gcttcagctg ctcgagttc. .......... 8509

8461 tctgcgagct ctcctaccac ctctaccgtt cgaagtcgac gagctcaag. .......... 8509

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-8509 Reference author: Honda S., Selker E.U.; Reference title: "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene Reference title: replacement, protein expression and protein purification"; Reference location: Genetics 0:0-0(2009).
Reference number: [2] Reference positions: 1-8509 Reference author: Honda S., Selker E.U.; Reference title: ; Reference location: Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA