lo8;lo8;"7ID FJ457013; SV 1; circular; other DNA; STD; SYN; 8817 BP. AC FJ457013; DT 30-JAN-2009 (Rel. 99, Created) DT 30-JAN-2009 (Rel. 99, Last updated, Version 1) DE Neurospora expression vector pCCG::Cre, complete sequence. KW . OS Neurospora expression vector pCCG::Cre OC other sequences; artificial sequences; vectors. RN [1] RP 1-8817 RA Honda S., Selker E.U.; RT "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene RT replacement, protein expression and protein purification"; RL Genetics 0:0-0(2009). RN [2] RP 1-8817 RA Honda S., Selker E.U.; RT ; RL Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. RL Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA FH Key Location/Qualifiers FT source 1..8817 FT /organism="Neurospora expression vector pCCG::Cre" FT /mol_type="other DNA" FT /db_xref="taxon:591007" FT CDS 468..1328 FT /codon_start=1 FT /transl_table=11 FT /product="beta-lactamase" FT /note="ampicillin resistance" FT /protein_id="ACM10020.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT misc_feature complement(2374..4006) FT /note="3' flank region of Neurospora crassa his-3 gene" FT promoter 4059..4980 FT /note="Neurospora crassa ccg-1 (grg-1)" FT CDS 4988..6043 FT /codon_start=1 FT /transl_table=11 FT /product="Cre" FT /protein_id="ACM10021.1" FT /translation="MGPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF FT SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLN FT MLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRC FT QDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALS FT LGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIY FT GAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSET FT GAMVRLLEDGD" FT misc_feature complement(6891..8798) FT /note="5' delta-his-3" SQ Sequence 8817 BP; 2188 A; 2280 C; 2287 G; 2062 T; 0 other; tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac 4020 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg 4080 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag 4140 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc 4200 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc 4260 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt 4320 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag 4380 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt 4440 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca 4500 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg 4560 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt 4620 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac 4680 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg 4740 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt 4800 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc 4860 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct 4920 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa 4980 ttctagaatg ggcccaaaga agaagagaaa ggtttcgaat ttactgaccg tacaccaaaa 5040 tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat 5100 gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg 5160 gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga 5220 tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca 5280 acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag 5340 caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg 5400 tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga 5460 aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac 5520 cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg 5580 tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga 5640 gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt 5700 agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc 5760 atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg 5820 attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag 5880 tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat 5940 gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag 6000 tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagccattaa cgcgcgacca 6060 gcttgatatc gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg 6120 tacccagctt ttgttccctt tagtgagggt taattagggc gtggacggct aatggggtct 6180 gaatgctaaa agacaccatt tcccacactc cctctgattt tttttatggt ttttttttat 6240 cttcattttt cgccgtcttt tattcggttg tttgggaggt tcacatgcaa cgtgcaagac 6300 tgcaagtttt ttgagatggg cgcttcatgc aatgcagcat cactgcaggg acattgtgac 6360 atccacgttt cgatcgtctg tgcttccccc gttcatgtac gcatgatctg aaaaaaaaaa 6420 aaaaaaaaaa ggttgatgta ataatgaaaa taaacaccca gggacgagca cctcatctgc 6480 tggaataggg agaaaaactc aagggccgtt tgggtctcca cacttaccaa cagattcatg 6540 tactgatctt cagcctgtac agaacagtac aatgtccaaa actcgttatc attactctat 6600 gtgcccaaaa tgacccagat atacatcaac ccactacatc cttttgttct tgtttttttt 6660 tttttttttt gctacttcga aaagaatttc cgtttagttg ctcttgctca tgtgctcaag 6720 acggatgctg accgcccttc tgtgagcctc gagctcctca accttagcca actgcatgac 6780 agcctggccg acgtttttga gaccctcggc agtcaagttg gagctggtaa tgtgcttgac 6840 gaacgaggcg aaattgacgc cagagtactg cttgccaaag ccataggtag ctatgtgtct 6900 tgacgttagc agaagaatct gtgaagcggg gctatttatg atatgtaact tacgcagcga 6960 gtggttaaca ccagcagagt aatcgccaac ggactcagga gtccaggcgc caatgaagac 7020 actaccggcg ttcatgacaa gatcgacagc cttctcggcc tccttgatct ggaggatcaa 7080 gtgctcagga gcgtacttgt tgctgagctc catggcctcc tcgacggtct tgacctgcac 7140 ggtgatcgag tgggcgatgg agccacggac aatctggacg cgaggaagct ccgtagcctg 7200 acggtgaacc tcgtcctcaa tagcctgaag atgctcctcg tcgaggtcaa tagcgatcag 7260 gatgacctga ctgtcaacgc cgtgctcagc ctgggacagg agatccgagg caacgaacgc 7320 ggggttggcg tccttgtcag cgatgaccag cacctcggac gggccagcgg gcatgtcaat 7380 accaacggca gcgttggtgt cgttgctgac gaacatcttg gcagcagtga cgaactggtt 7440 accggggccg agaatcttgt cgaccttggt gatgctctcg gtgccgtagg ccatggcagc 7500 tacggcctgg gcaccgccgg caagcacgat ggactcggcc ccaaccttgt gagcgacgtg 7560 gacaatctcg ggagtgatgg ttccgtcggc gcggggagga gaggcgaaca caatcttgtt 7620 gcagccggcg accatggcgg gaacacccag cataagggca gtgctgggga gaacggcggt 7680 accgccgggg atgtagcagc cgacggcctc gatgggacga gagaaacggc tgcagacaac 7740 accgggcatg gtctcgacct ggaggggctt ctcctccttc tgggcggcgt ggaacttgcg 7800 gatgttctcg aaggacacgt cgatggcagc aatggtctcc tcagggagct gcataagctc 7860 cttggggaag ggcgccttca ggacggggct agtaagagag gtagccttct cgaacttgtg 7920 agtgtacgac agaacagcct tgtcgccgtt cttgcggacg tcctcgatga tggggacaat 7980 gatcttgtag atggcatcgg acgacttttg cgcaggacgc ttgagagcag catcgagctc 8040 ctcggtagag accttggagg cgtcgaaacg tctcatggtg atcttctcag gggtggtctc 8100 ctgggcggcc tccttggtga caggggccga agtggcagcg agagcggacg ccgcaggctt 8160 gatgccctcc ttctcagccc acttaccctt agcatctcca gtcctgcgct tgaccttcca 8220 gctcttggcg tcaaggctcc tttcgatatc ggcaagagta acgccggcag caacggccct 8280 ggtaagagca aagtagaaga gatcggcagc ctcaaaggcg atttcctggg gggtctgagc 8340 ggtgcagagc tcctcagcct cctccatgat cttggcccgg actagcttct catcggagaa 8400 gagacgggca gtgtaggagc cctcgggggc agactgtttc ctcgaaatca aagtctgctc 8460 gagcttggga aggcctttga gctgaccaaa gcagccggac tgatcgaggt ggcagaaacg 8520 acccttctgc ttcacgacaa acttgagagc atcgttatcg cagtcaagcg agatgcggac 8580 gagctcctga gtgtctccgg aagtagcacc cttgtaccag agaccgcgct tccggctctg 8640 atagacacca gtctgtgtcc tgagggcctc gttcacactc tcggcactgc tgtagaccag 8700 acccagagca gtgtcgtgct catcaacgac aacggtgggg agaagaccat cgggacggtc 8760 agacttccag acgctcgaga ggatggtgga gatggcaagc ttcagctgct cgagttc 8817 q" pCCG::Cre"Times New Roman lo8;NdeI ArialRq"^o^e^[IOs[ lo8;ScaI Arialq"~r~ lo8;+HpaI(2327),KpnI(2480),NheI(2669),ClaI(2831) Arial q"3=FFk} lo8;7HpaI(3320),ScaI(3640),XhoI(3652),XhoI(3833),PvuII(3881) ArialDq""** f lo8;MSacI(4040),EagI(4053),NotI(4053),ClaI(4111),MluI(4248),SexAI(4325),NheI(4446) Arialq"5>G\G lo8;:HindIII(4796),XbaI(4983),ApaI(4995),NruI(5227),BamHI(5368) Arialq"(1:: lo8;"SexAI(5440),EcoRV(5564),ClaI(5819) Arialq"Z" lo8;sEcoRV(6068),EcoRI(6072),EcoRV(6080),HindIII(6084),ClaI(6091),SalI(6099),XhoI(6105),ApaI(6118),KpnI(6124),PstI(6348) Arial ^q"|si)ui lo8;AXhoI(6749),SacI(6756),ScaI(6867),HpaI(6967),SacI(7110),NcoI(7111) Arialq"o-e+[)$[M lo8;NSalI(7460),NcoI(7492),NcoI(7633),KpnI(7683),PstI(7735),Bsu36I(7841),SacI(8040) Arialq"ZV lo8;iEcoRV(8248),SacI(8352),XhoI(8459),SacI(8586),Bsu36I(8661),XhoI(8775),HindIII(8798),PvuII(8806),XhoI(8810) Arial!q"5p3^0LjLlo8;CɆq" Sp6_primer"Times New Romanlo8;CɆq" pGEX_3_primer"Times New Romanlo8;Cq" AmpR_promoter"Times New Romanlo8;Cq"0 Ampicillin"Times New Romanlo8;q"0ORF 3"Times New Romanlo8;Cq"6 pBR322_origin"Times New Romanlo8;q") ;  T7_promoter"Times New Romanlo8;Cq" T7_promoter"Times New Romanlo8;Cq"ORF 2"Times New Romanlo8;Cq"pBluescriptKS_primer"Times New Romanlo8;q"  T3_promoter"Times New Romanlo8;CɆq"& ORF 1"Times New Romanlo8;q"? ORF 1"Times New Roman lo8;test"Times New RomanAq"tkaEah lo8;test"Times New Romanq"yH4