bPbPB1ID FJ457004; SV 1; circular; other DNA; STD; SYN; 7766 BP. AC FJ457004; DT 30-JAN-2009 (Rel. 99, Created) DT 30-JAN-2009 (Rel. 99, Last updated, Version 1) DE Neurospora expression vector pCCG::N-3xFLAG, complete sequence. KW . OS Neurospora expression vector pCCG::N-3xFLAG OC other sequences; artificial sequences; vectors. RN [1] RP 1-7766 RA Honda S., Selker E.U.; RT "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene RT replacement, protein expression and protein purification"; RL Genetics 0:0-0(2009). RN [2] RP 1-7766 RA Honda S., Selker E.U.; RT ; RL Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. RL Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA FH Key Location/Qualifiers FT source 1..7766 FT /organism="Neurospora expression vector pCCG::N-3xFLAG" FT /mol_type="other DNA" FT /db_xref="taxon:591008" FT promoter complement(1..17) FT /note="SP6" FT CDS 468..1328 FT /codon_start=1 FT /transl_table=11 FT /product="beta-lactamase" FT /note="ampicillin resistance" FT /protein_id="ACM10005.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT promoter complement(2345..2365) FT /note="T7" FT misc_feature complement(2374..4006) FT /note="3' flank region of Neurospora crassa his-3 gene" FT promoter 4092..4934 FT /note="Neurospora crassa ccg-1 (grg-1)" FT misc_feature 4936..5004 FT /note="3xFLAG" FT misc_feature 5005..5028 FT /note="8xFLAG" FT misc_feature complement(5646..7747) FT /note="5' truncated histidine biosynthesis trifunctional FT protein; includes phosphoribosyl-AMP cyclohydrolase, FT phosphoribosyl-ATP cyclohydrolase and histindinol FT dehydrogenase" SQ Sequence 7766 BP; 1927 A; 2041 C; 2001 G; 1797 T; 0 other; tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac 4020 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt 4080 gatatcgaat tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg 4140 gacgagaccg acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg 4200 tcggccttcg gtaacagaaa agcacacaga cagcgattgt gacatggatt cgggcaaagc 4260 attggtggtc gcaccaggtc atcctgagtg tgcagtggct gctattcaga tttcatctaa 4320 ctgcgggaga ggggttcaaa ggggcgtgac gtcacagaca acgggtgaag gacgaagatt 4380 gcctcacttc tttgctagca atttgcctgc aaagaaggcg cacatgacaa gcaaacaact 4440 gggaaaccac tattgaatac ccacaatgca aagctcggaa ggtacgtctt gattgcagtg 4500 tgtcgagtgt caaaaaagaa gcaagtgttc atgcaagcca aaattggcac ctcctccact 4560 tctccgagtg ccccacccga acctccaggc gagatggccg gaacatacca tccgcgttgg 4620 gattatgacg tatctccttc ttcttcacat gattccatcc cgttgttgct tgtttgcgag 4680 ctgtgacggg agatcgtaga tgccacttcg ggccaggcag gcagtgcagg cagccaggaa 4740 cacaagcttc caacttggtc atctcgattg ccgattaagg gaaccaaatg cctatataag 4800 actgtcctcc cacctcccca ataccattct tttcttcttc catcatcagc caacaaagca 4860 atcacatctt cactacttca aatcaacaca acactcaaac cactttcaca acccctcaca 4920 tcaaccaaag gatccatgga ctacaaagac catgacggtg attataaaga tcatgacatc 4980 gactacaagg atgacgatga caagggcggc ggaggcggcg gaggcggcgc gccattaatt 5040 aactagttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt 5100 attaagggcg tggacggcta atggggtctg aatgctaaaa gacaccattt cccacactcc 5160 ctctgatttt ttttatggtt tttttttatc ttcatttttc gccgtctttt attcggttgt 5220 ttgggaggtt cacatgcaac gtgcaagact gcaagttttt tgagatgggc gcttcatgca 5280 atgcagcatc actgcaggga cattgtgaca tccacgtttc gatcgtctgt gcttcccccg 5340 ttcatgtacg catgatctga aaaaaaaaaa aaaaaaaaag gttgatgtaa taatgaaaat 5400 aaacacccag ggacgagcac ctcatctgct ggaataggga gaaaaactca agggccgttt 5460 gggtctccac acttaccaac agattcatgt actgatcttc agcctgtaca gaacagtaca 5520 atgtccaaaa ctcgttatca ttactctatg tgcccaaaat gacccagata tacatcaacc 5580 cactacatcc ttttgttctt gttttttttt tttttttttg ctacttcgaa aagaatttcc 5640 gtttagttgc tcttgctcat gtgctcaaga cggatgctga ccgcccttct gtgagcctcg 5700 agctcctcaa ccttagccaa ctgcatgaca gcctggccga cgtttttgag accctcggca 5760 gtcaagttgg agctggtaat gtgcttgacg aacgaggcga aattgacgcc agagtactgc 5820 ttgccaaagc cataggtagc tatgtgtctt gacgttagca gaagaatctg tgaagcgggg 5880 ctatttatga tatgtaactt acgcagcgag tggttaacac cagcagagta atcgccaacg 5940 gactcaggag tccaggcgcc aatgaagaca ctaccggcgt tcatgacaag atcgacagcc 6000 ttctcggcct ccttgatctg gaggatcaag tgctcaggag cgtacttgtt gctgagctcc 6060 atggcctcct cgacggtctt gacctgcacg gtgatcgagt gggcgatgga gccacggaca 6120 atctggacgc gaggaagctc cgtagcctga cggtgaacct cgtcctcaat agcctgaaga 6180 tgctcctcgt cgaggtcaat agcgatcagg atgacctgac tgtcaacgcc gtgctcagcc 6240 tgggacagga gatccgaggc aacgaacgcg gggttggcgt ccttgtcagc gatgaccagc 6300 acctcggacg ggccagcggg catgtcaata ccaacggcag cgttggtgtc gttgctgacg 6360 aacatcttgg cagcagtgac gaactggtta ccggggccga gaatcttgtc gaccttggtg 6420 atgctctcgg tgccgtaggc catggcagct acggcctggg caccgccggc aagcacgatg 6480 gactcggccc caaccttgtg agcgacgtgg acaatctcgg gagtgatggt tccgtcggcg 6540 cggggaggag aggcgaacac aatcttgttg cagccggcga ccatggcggg aacacccagc 6600 ataagggcag tgctggggag aacggcggta ccgccgggga tgtagcagcc gacggcctcg 6660 atgggacgag agaaacggct gcagacaaca ccgggcatgg tctcgacctg gaggggcttc 6720 tcctccttct gggcggcgtg gaacttgcgg atgttctcga aggacacgtc gatggcagca 6780 atggtctcct cagggagctg cataagctcc ttggggaagg gcgccttcag gacggggcta 6840 gtaagagagg tagccttctc gaacttgtga gtgtacgaca gaacagcctt gtcgccgttc 6900 ttgcggacgt cctcgatgat ggggacaatg atcttgtaga tggcatcgga cgacttttgc 6960 gcaggacgct tgagagcagc atcgagctcc tcggtagaga ccttggaggc gtcgaaacgt 7020 ctcatggtga tcttctcagg ggtggtctcc tgggcggcct ccttggtgac aggggccgaa 7080 gtggcagcga gagcggacgc cgcaggcttg atgccctcct tctcagccca cttaccctta 7140 gcatctccag tcctgcgctt gaccttccag ctcttggcgt caaggctcct ttcgatatcg 7200 gcaagagtaa cgccggcagc aacggccctg gtaagagcaa agtagaagag atcggcagcc 7260 tcaaaggcga tttcctgggg ggtctgagcg gtgcagagct cctcagcctc ctccatgatc 7320 ttggcccgga ctagcttctc atcggagaag agacgggcag tgtaggagcc ctcgggggca 7380 gactgtttcc tcgaaatcaa agtctgctcg agcttgggaa ggcctttgag ctgaccaaag 7440 cagccggact gatcgaggtg gcagaaacga cccttctgct tcacgacaaa cttgagagca 7500 tcgttatcgc agtcaagcga gatgcggacg agctcctgag tgtctccgga agtagcaccc 7560 ttgtaccaga gaccgcgctt ccggctctga tagacaccag tctgtgtcct gagggcctcg 7620 ttcacactct cggcactgct gtagaccaga cccagagcag tgtcgtgctc atcaacgaca 7680 acggtgggga gaagaccatc gggacggtca gacttccaga cgctcgagag gatggtggag 7740 atggcaagct tcagctgctc gagttc 7766 VpCCG::N-3xFLAG"Times New Roman bPNdeI ArialRVzpsdp bPScaI ArialV  8 bP+HpaI(2327),KpnI(2480),NheI(2669),ClaI(2831) Arial VNW`` bP+HpaI(3320),ScaI(3640),XhoI(3652),XhoI(3833) Arial8VJT]] bP͊PvuII(3881),KpnI(4044),ApaI(4050),XhoI(4055),SalI(4061),ClaI(4071),HindIII(4076),EcoRV(4084),EcoRI(4088),MluI(4197),SexAI(4274),NheI(4395) ArialV[MQ:x:xz bPqHindIII(4745),BamHI(4931),NcoI(4935),AscI(5028),PacI(5040),SpeI(5043),XbaI(5049),EagI(5056),NotI(5056),SacI(5077) Arial Ve bP PstI(5297),XhoI(5698),SacI(5705) ArialBVMr bP+ScaI(5816),HpaI(5916),SacI(6059),NcoI(6060) ArialVYYXK@p bPCSalI(6409),NcoI(6441),NcoI(6582),KpnI(6632),PstI(6684),Bsu36I(6790) ArialVn bP,SacI(6989),EcoRV(7197),SacI(7301),XhoI(7408) ArialV [  bPGSacI(7535),Bsu36I(7610),XhoI(7724),HindIII(7747),PvuII(7755),XhoI(7759) Arial,Vssrdd$ddbPV Sp6_primer"Times New RomanbPCV pGEX_3_primer"Times New RomanbPCV AmpR_promoter"Times New RomanbPCV0 Ampicillin"Times New RomanbPCV0ORF 1"Times New RomanbPV6 pBR322_origin"Times New RomanbPCɆV) ;  T7_promoter"Times New RomanbPCV T3_promoter"Times New RomanbPCɆVpBluescriptKS_primer"Times New RomanbPCV~EK"Times New RomanbPCV T7_promoter"Times New RomanbPCV ORF 1"Times New RomanbPCV$ORF 2"Times New Roman bPtest"Times New RomanAVxjVh