Sequence of pCCG

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Sequence of pCCG_N-3xFLAG

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Sequence of pCCG::N-3xFLAG: 7766 BP; 1927 A; 2041 C; 2001 G; 1797 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60

1 atatcacagt ggatttagca tacacatact atgtattcca atacataatt aacatcggcg 60

Sp6_primer .......... .......... .......... .......... ..........

.......... .......... NdeI(82) .......... .......... ..........

61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120

61 caagattgct gttatacagg tataccacgt gagagtcatg ttagacgaga ctacggcgta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180

121 tcaattcggt cggggctgtg ggcggttgtg ggcgactgcg cgggactgcc cgaacagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240

181 agggccgtag gcgaatgtct gttcgacact ggcagaggcc ctcgacgtac acagtctcca 240

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300

241 aaagtggcag tagtggcttt gcgcgctctg ctttcccgga gcactatgcg gataaaaata 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360

301 tccaattaca gtactattat taccaaagaa tctgcagtcc accgtgaaaa gcccctttac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420

361 acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata ggcgagtact 420

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480

421 ctgttattgg gactatttac gaagttatta taactttttc cttctcatac tcataagttg 480

.......... .......... .......... .......... ....... Ampicillin ORF 1

.......... .......... .......... .......... .......... ..........

481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540

481 taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg 540

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600

541 gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt 600

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660

601 agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag 660

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720

661 gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca taactgcggc 720

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ScaI(775)

721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780

721 ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg 780

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840

781 gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt 840

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900

841 attggtactc actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc 900

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960

901 tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg 960

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020

961 gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga catcgttacc 1020

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080

1021 gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta 1080

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140

1081 attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc 1140

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200

1141 gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac 1200

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260

1201 gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag 1260

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320

1261 tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg 1320

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380

1321 taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440

1381 aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500

1441 ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa 1500

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560

1501 ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620

1561 gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680

1621 cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740

1681 tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800

1741 ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860

1801 gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920

1861 tgtggcttga ctctatggat gtcgcactcg taactctttc gcggtgcgaa gggcttccct 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980

1921 ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040

1981 aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100

2041 tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160

2101 gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220

2161 ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280

2221 cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcgggttatg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340

2281 cgtttggcgg agaggggcgc gcaaccggct aagtaattac gtccaattgg accgaatagc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400

2341 tttaattatg ctgagtgata tccctctggc cgtctaggtt acgcctacct aagcgagtga 2400

......... T7_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460

2401 gccacggcgc cgccacgctt caaggacggc acctaacccc ctaacgtgtc tgccacctca 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520

2461 cgaacgatgt cgatccatgg gtcaacgtgt ggtttgccac ggggacacgg ggacgcagat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580

2521 ccacacagga cactcgttga cgcgggcgcg cgccgtggcc cttcgacgcc cttggagtta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640

2581 cctactaacc gcggtctagg ggccgtagtg gagtcgtgta cccgtcgggc tcttccttag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700

2641 ctcccatctt ctataaggtc ccaaaggcga tcgtgttgaa ggttcgcgga gatggcgatg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760

2701 aggtgtgtgg gtgcgactta ccgtgttggt gacatccgga tcgccagggt cgaggggcat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820

2761 tggtgcgtcg gggtcgctgg ctccagtagt actgtgatac tgctgttaga ccgccctctg 2820

.......... .......... .......... .......... .......... ..........

.......... ClaI(2831) .......... .......... .......... ..........

2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880

2821 aaccctttta gctactgccc gtggatggaa cccttgttat ctcgttcagg tcagttgcgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940

2881 gatcccttcc cccttttttt ttttctcggg taagagtgtt cctcgttgtg acacgacgat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000

2941 agtatcatgt gatgttcgtt tcagccctac aacgatctta ggcagccgat gcagccatct 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060

3001 atggagggta tccgtcattc agatccatag gttaaatatc aaggcgcata aagaagcagc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120

3061 ttgccccgtg tcgtttcgga ttgtcggcgt cacggagcgt tggttgtggt tcgtcattag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180

3121 agtaggtagg tatgtctagg agggtgttat agaaatgtac gcgtgaacgc gacacaatcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240

3181 tcaaatccaa ggtgatggtt ccgactgtgc tcgtcgtgac tgtcgtttga cgacgaaccc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300

3241 cggttcttta accgtcaaac gactcgtgga tgtatagtca tgtgtagttg ggaccaaatt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360

3301 aacaatcgga ccatgacaat tggcagtact tgctcctgca cctccttctt ggtgcgattg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420

3361 ttaacttggt cccaactaca tatggagaga atggtataaa cgaacgaagg tgcagggata 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480

3421 ggagttcgct aaaacaagaa ctgaaaaaca taaagttgcg tttccggtaa gtccaaagag 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540

3481 tacaagttgg gaaacctacc gctacggcaa gcagggtcct ggagcactgg aaactttctg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600

3541 ctctttatat agggtttcgt tgaagacgaa tacctgttcg ttgacgtacc gccgggtgtt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ScaI(3640) .......... ..........

3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660

3601 gagtatagag cgcattagta ggtttgcgat ccgggctcat gagatgacaa gagctctgtg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720

3661 gatggtatta tacatgggtt agtctgtact gttgtcagtt ggtttgtatg ggactaggcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780

3721 ggtttgggac tgtagggatc tactaccaat ccgtgtgttt accttttgcc ctgagcaacc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... XhoI(3833)

3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840

3781 aatccaccct tgcgaacata acgtcggtcg gttggctgag aggagcaggg agagctcagg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900

3841 gcaataacgg caaactgggt cggcgggtcg gcggtttgtc gactccctcg gttaccagat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960

3901 tatgtggcag gttgccgggg tcactacaaa gtttgaacta actgtcgctt gctttgggga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac 4020

3961 ctttgggcaa tatctataca tatctaaact gccccatcga accgggatta attgggagtg 4020

.......... .......... .......... .......... ......... T3_promoter

.......... .... KpnI(4044) ApaI(4050),XhoI(4055) SalI(4061) ....

4021 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt 4080

4021 atttcccttg ttttcgaccc atggcccggg ggggagctcc agctgccata gctattcgaa 4080

.......... .......... .......... ..... pBluescriptKS_primer .....

.......... .......... .......... .......... .......... ..........

4081 gatatcgaat tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg 4140

4081 ctatagctta agcaagtttc ggtgtagtga cccgtgaagg taaccctgtc cgtaactagc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gacgagaccg acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg 4200

4141 ctgctctggc tgaagaccgg cgaaagttgt cggtgtaata taggtacagt gccgatgcgc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tcggccttcg gtaacagaaa agcacacaga cagcgattgt gacatggatt cgggcaaagc 4260

4201 agccggaagc cattgtcttt tcgtgtgtct gtcgctaaca ctgtacctaa gcccgtttcg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 attggtggtc gcaccaggtc atcctgagtg tgcagtggct gctattcaga tttcatctaa 4320

4261 taaccaccag cgtggtccag taggactcac acgtcaccga cgataagtct aaagtagatt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctgcgggaga ggggttcaaa ggggcgtgac gtcacagaca acgggtgaag gacgaagatt 4380

4321 gacgccctct ccccaagttt ccccgcactg cagtgtctgt tgcccacttc ctgcttctaa 4380

.......... .......... .......... .......... .......... ..........

.......... NheI(4395) .......... .......... .......... ..........

4381 gcctcacttc tttgctagca atttgcctgc aaagaaggcg cacatgacaa gcaaacaact 4440

4381 cggagtgaag aaacgatcgt taaacggacg tttcttccgc gtgtactgtt cgtttgttga 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gggaaaccac tattgaatac ccacaatgca aagctcggaa ggtacgtctt gattgcagtg 4500

4441 ccctttggtg ataacttatg ggtgttacgt ttcgagcctt ccatgcagaa ctaacgtcac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgtcgagtgt caaaaaagaa gcaagtgttc atgcaagcca aaattggcac ctcctccact 4560

4501 acagctcaca gttttttctt cgttcacaag tacgttcggt tttaaccgtg gaggaggtga 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tctccgagtg ccccacccga acctccaggc gagatggccg gaacatacca tccgcgttgg 4620

4561 agaggctcac ggggtgggct tggaggtccg ctctaccggc cttgtatggt aggcgcaacc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gattatgacg tatctccttc ttcttcacat gattccatcc cgttgttgct tgtttgcgag 4680

4621 ctaatactgc atagaggaag aagaagtgta ctaaggtagg gcaacaacga acaaacgctc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctgtgacggg agatcgtaga tgccacttcg ggccaggcag gcagtgcagg cagccaggaa 4740

4681 gacactgccc tctagcatct acggtgaagc ccggtccgtc cgtcacgtcc gtcggtcctt 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cacaagcttc caacttggtc atctcgattg ccgattaagg gaaccaaatg cctatataag 4800

4741 gtgttcgaag gttgaaccag tagagctaac ggctaattcc cttggtttac ggatatattc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 actgtcctcc cacctcccca ataccattct tttcttcttc catcatcagc caacaaagca 4860

4801 tgacaggagg gtggaggggt tatggtaaga aaagaagaag gtagtagtcg gttgtttcgt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atcacatctt cactacttca aatcaacaca acactcaaac cactttcaca acccctcaca 4920

4861 tagtgtagaa gtgatgaagt ttagttgtgt tgtgagtttg gtgaaagtgt tggggagtgt 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcaaccaaag gatccatgga ctacaaagac catgacggtg attataaaga tcatgacatc 4980

4921 agttggtttc ctaggtacct gatgtttctg gtactgccac taatatttct agtactgtag 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AscI(5028) ..........

4981 gactacaagg atgacgatga caagggcggc ggaggcggcg gaggcggcgc gccattaatt 5040

4981 ctgatgttcc tactgctact gttcccgccg cctccgccgc ctccgccgcg cggtaattaa 5040

.......... EK .......... .......... .......... ..........

SpeI) XbaI(5049) EagI(5056),NotI(5056) SacI(5077) ......

5041 aactagttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt 5100

5041 ttgatcaaga tctcgccggc ggtggcgcca cctcgaggtt aagcgggata tcactcagca 5100

.......... .......... .......... .......... ......... T7_promoter

.......... .......... .......... .......... .......... ..........

5101 attaagggcg tggacggcta atggggtctg aatgctaaaa gacaccattt cccacactcc 5160

5101 taattcccgc acctgccgat taccccagac ttacgatttt ctgtggtaaa gggtgtgagg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctctgatttt ttttatggtt tttttttatc ttcatttttc gccgtctttt attcggttgt 5220

5161 gagactaaaa aaaataccaa aaaaaaatag aagtaaaaag cggcagaaaa taagccaaca 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttgggaggtt cacatgcaac gtgcaagact gcaagttttt tgagatgggc gcttcatgca 5280

5221 aaccctccaa gtgtacgttg cacgttctga cgttcaaaaa actctacccg cgaagtacgt 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atgcagcatc actgcaggga cattgtgaca tccacgtttc gatcgtctgt gcttcccccg 5340

5281 tacgtcgtag tgacgtccct gtaacactgt aggtgcaaag ctagcagaca cgaagggggc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttcatgtacg catgatctga aaaaaaaaaa aaaaaaaaag gttgatgtaa taatgaaaat 5400

5341 aagtacatgc gtactagact tttttttttt tttttttttc caactacatt attactttta 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaacacccag ggacgagcac ctcatctgct ggaataggga gaaaaactca agggccgttt 5460

5401 tttgtgggtc cctgctcgtg gagtagacga ccttatccct ctttttgagt tcccggcaaa 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gggtctccac acttaccaac agattcatgt actgatcttc agcctgtaca gaacagtaca 5520

5461 cccagaggtg tgaatggttg tctaagtaca tgactagaag tcggacatgt cttgtcatgt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 atgtccaaaa ctcgttatca ttactctatg tgcccaaaat gacccagata tacatcaacc 5580

5521 tacaggtttt gagcaatagt aatgagatac acgggtttta ctgggtctat atgtagttgg 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cactacatcc ttttgttctt gttttttttt tttttttttg ctacttcgaa aagaatttcc 5640

5581 gtgatgtagg aaaacaagaa caaaaaaaaa aaaaaaaaac gatgaagctt ttcttaaagg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gtttagttgc tcttgctcat gtgctcaaga cggatgctga ccgcccttct gtgagcctcg 5700

5641 caaatcaacg agaacgagta cacgagttct gcctacgact ggcgggaaga cactcggagc 5700

.......... .......... .......... ORF 1 .......... ..........

SacI(5705) .......... .......... .......... .......... ..........

5701 agctcctcaa ccttagccaa ctgcatgaca gcctggccga cgtttttgag accctcggca 5760

5701 tcgaggagtt ggaatcggtt gacgtactgt cggaccggct gcaaaaactc tgggagccgt 5760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gtcaagttgg agctggtaat gtgcttgacg aacgaggcga aattgacgcc agagtactgc 5820

5761 cagttcaacc tcgaccatta cacgaactgc ttgctccgct ttaactgcgg tctcatgacg 5820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttgccaaagc cataggtagc tatgtgtctt gacgttagca gaagaatctg tgaagcgggg 5880

5821 aacggtttcg gtatccatcg atacacagaa ctgcaatcgt cttcttagac acttcgcccc 5880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ctatttatga tatgtaactt acgcagcgag tggttaacac cagcagagta atcgccaacg 5940

5881 gataaatact atacattgaa tgcgtcgctc accaattgtg gtcgtctcat tagcggttgc 5940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gactcaggag tccaggcgcc aatgaagaca ctaccggcgt tcatgacaag atcgacagcc 6000

5941 ctgagtcctc aggtccgcgg ttacttctgt gatggccgca agtactgttc tagctgtcgg 6000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .... SacI(6059),NcoI(6060)

6001 ttctcggcct ccttgatctg gaggatcaag tgctcaggag cgtacttgtt gctgagctcc 6060

6001 aagagccgga ggaactagac ctcctagttc acgagtcctc gcatgaacaa cgactcgagg 6060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 atggcctcct cgacggtctt gacctgcacg gtgatcgagt gggcgatgga gccacggaca 6120

6061 taccggagga gctgccagaa ctggacgtgc cactagctca cccgctacct cggtgcctgt 6120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 atctggacgc gaggaagctc cgtagcctga cggtgaacct cgtcctcaat agcctgaaga 6180

6121 tagacctgcg ctccttcgag gcatcggact gccacttgga gcaggagtta tcggacttct 6180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tgctcctcgt cgaggtcaat agcgatcagg atgacctgac tgtcaacgcc gtgctcagcc 6240

6181 acgaggagca gctccagtta tcgctagtcc tactggactg acagttgcgg cacgagtcgg 6240

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tgggacagga gatccgaggc aacgaacgcg gggttggcgt ccttgtcagc gatgaccagc 6300

6241 accctgtcct ctaggctccg ttgcttgcgc cccaaccgca ggaacagtcg ctactggtcg 6300

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 acctcggacg ggccagcggg catgtcaata ccaacggcag cgttggtgtc gttgctgacg 6360

6301 tggagcctgc ccggtcgccc gtacagttat ggttgccgtc gcaaccacag caacgactgc 6360

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 aacatcttgg cagcagtgac gaactggtta ccggggccga gaatcttgtc gaccttggtg 6420

6361 ttgtagaacc gtcgtcactg cttgaccaat ggccccggct cttagaacag ctggaaccac 6420

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atgctctcgg tgccgtaggc catggcagct acggcctggg caccgccggc aagcacgatg 6480

6421 tacgagagcc acggcatccg gtaccgtcga tgccggaccc gtggcggccg ttcgtgctac 6480

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gactcggccc caaccttgtg agcgacgtgg acaatctcgg gagtgatggt tccgtcggcg 6540

6481 ctgagccggg gttggaacac tcgctgcacc tgttagagcc ctcactacca aggcagccgc 6540

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cggggaggag aggcgaacac aatcttgttg cagccggcga ccatggcggg aacacccagc 6600

6541 gcccctcctc tccgcttgtg ttagaacaac gtcggccgct ggtaccgccc ttgtgggtcg 6600

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ataagggcag tgctggggag aacggcggta ccgccgggga tgtagcagcc gacggcctcg 6660

6601 tattcccgtc acgacccctc ttgccgccat ggcggcccct acatcgtcgg ctgccggagc 6660

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 atgggacgag agaaacggct gcagacaaca ccgggcatgg tctcgacctg gaggggcttc 6720

6661 taccctgctc tctttgccga cgtctgttgt ggcccgtacc agagctggac ctccccgaag 6720

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tcctccttct gggcggcgtg gaacttgcgg atgttctcga aggacacgtc gatggcagca 6780

6721 aggaggaaga cccgccgcac cttgaacgcc tacaagagct tcctgtgcag ctaccgtcgt 6780

.......... ......... ORF 1 ORF 2 ......... .......... ..........

Bsu36I(6790) ........ .......... .......... .......... ..........

6781 atggtctcct cagggagctg cataagctcc ttggggaagg gcgccttcag gacggggcta 6840

6781 taccagagga gtccctcgac gtattcgagg aaccccttcc cgcggaagtc ctgccccgat 6840

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gtaagagagg tagccttctc gaacttgtga gtgtacgaca gaacagcctt gtcgccgttc 6900

6841 cattctctcc atcggaagag cttgaacact cacatgctgt cttgtcggaa cagcggcaag 6900

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ttgcggacgt cctcgatgat ggggacaatg atcttgtaga tggcatcgga cgacttttgc 6960

6901 aacgcctgca ggagctacta cccctgttac tagaacatct accgtagcct gctgaaaacg 6960

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gcaggacgct tgagagcagc atcgagctcc tcggtagaga ccttggaggc gtcgaaacgt 7020

6961 cgtcctgcga actctcgtcg tagctcgagg agccatctct ggaacctccg cagctttgca 7020

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ctcatggtga tcttctcagg ggtggtctcc tgggcggcct ccttggtgac aggggccgaa 7080

7021 gagtaccact agaagagtcc ccaccagagg acccgccgga ggaaccactg tccccggctt 7080

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gtggcagcga gagcggacgc cgcaggcttg atgccctcct tctcagccca cttaccctta 7140

7081 caccgtcgct ctcgcctgcg gcgtccgaac tacgggagga agagtcgggt gaatgggaat 7140

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 gcatctccag tcctgcgctt gaccttccag ctcttggcgt caaggctcct ttcgatatcg 7200

7141 cgtagaggtc aggacgcgaa ctggaaggtc gagaaccgca gttccgagga aagctatagc 7200

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gcaagagtaa cgccggcagc aacggccctg gtaagagcaa agtagaagag atcggcagcc 7260

7201 cgttctcatt gcggccgtcg ttgccgggac cattctcgtt tcatcttctc tagccgtcgg 7260

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tcaaaggcga tttcctgggg ggtctgagcg gtgcagagct cctcagcctc ctccatgatc 7320

7261 agtttccgct aaaggacccc ccagactcgc cacgtctcga ggagtcggag gaggtactag 7320

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ttggcccgga ctagcttctc atcggagaag agacgggcag tgtaggagcc ctcgggggca 7380

7321 aaccgggcct gatcgaagag tagcctcttc tctgcccgtc acatcctcgg gagcccccgt 7380

ORF 2 .......... .......... .......... .......... ..........

.......... .......... XhoI(7408) .......... .......... ..........

7381 gactgtttcc tcgaaatcaa agtctgctcg agcttgggaa ggcctttgag ctgaccaaag 7440

7381 ctgacaaagg agctttagtt tcagacgagc tcgaaccctt ccggaaactc gactggtttc 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cagccggact gatcgaggtg gcagaaacga cccttctgct tcacgacaaa cttgagagca 7500

7441 gtcggcctga ctagctccac cgtctttgct gggaagacga agtgctgttt gaactctcgt 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tcgttatcgc agtcaagcga gatgcggacg agctcctgag tgtctccgga agtagcaccc 7560

7501 agcaatagcg tcagttcgct ctacgcctgc tcgaggactc acagaggcct tcatcgtggg 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ttgtaccaga gaccgcgctt ccggctctga tagacaccag tctgtgtcct gagggcctcg 7620

7561 aacatggtct ctggcgcgaa ggccgagact atctgtggtc agacacagga ctcccggagc 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ttcacactct cggcactgct gtagaccaga cccagagcag tgtcgtgctc atcaacgaca 7680

7621 aagtgtgaga gccgtgacga catctggtct gggtctcgtc acagcacgag tagttgctgt 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 acggtgggga gaagaccatc gggacggtca gacttccaga cgctcgagag gatggtggag 7740

7681 tgccacccct cttctggtag ccctgccagt ctgaaggtct gcgagctctc ctaccacctc 7740

.......... .......... .......... .......... .......... ..........

HindIII(7747) PvuII(7755),XhoI(7759) .... .......... ..........

7741 atggcaagct tcagctgctc gagttc.... .......... .......... .......... 7766

7741 taccgttcga agtcgacgag ctcaag.... .......... .......... .......... 7766

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-7766 Reference author: Honda S., Selker E.U.; Reference title: "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene Reference title: replacement, protein expression and protein purification"; Reference location: Genetics 0:0-0(2009).
Reference number: [2] Reference positions: 1-7766 Reference author: Honda S., Selker E.U.; Reference title: ; Reference location: Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA