OO0ID FJ457005; SV 1; circular; other DNA; STD; SYN; 7790 BP. AC FJ457005; DT 30-JAN-2009 (Rel. 99, Created) DT 30-JAN-2009 (Rel. 99, Last updated, Version 1) DE Neurospora expression vector pCCG::N-3xMyc, complete sequence. KW . OS Neurospora expression vector pCCG::N-3xMyc OC other sequences; artificial sequences; vectors. RN [1] RP 1-7790 RA Honda S., Selker E.U.; RT "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene RT replacement, protein expression and protein purification"; RL Genetics 0:0-0(2009). RN [2] RP 1-7790 RA Honda S., Selker E.U.; RT ; RL Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. RL Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA FH Key Location/Qualifiers FT source 1..7790 FT /organism="Neurospora expression vector pCCG::N-3xMyc" FT /mol_type="other DNA" FT /db_xref="taxon:591009" FT CDS 468..1328 FT /codon_start=1 FT /transl_table=11 FT /product="beta-lactamase" FT /note="ampicillin resistance" FT /protein_id="ACM10006.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT promoter complement(2345..2365) FT /note="T7" FT misc_feature complement(2374..4006) FT /note="3' flank region of Neurospora crassa his-3 gene" FT promoter 4092..4934 FT /note="Neurospora crassa ccg-1 (grg-1)" FT misc_feature 4936..5028 FT /note="3xMyc" FT misc_feature 5029..5052 FT /note="8xGly" FT misc_feature complement(5670..7771) FT /note="5' truncated histidine biosynthesis trifunctional FT protein; includes phosphoribosyl-AMP cyclohydrolase, FT phosphoribosyl-ATP cyclohydrolase and histindinol FT dehydrogenase" SQ Sequence 7790 BP; 1939 A; 2041 C; 2005 G; 1805 T; 0 other; tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac 4020 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt 4080 gatatcgaat tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg 4140 gacgagaccg acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg 4200 tcggccttcg gtaacagaaa agcacacaga cagcgattgt gacatggatt cgggcaaagc 4260 attggtggtc gcaccaggtc atcctgagtg tgcagtggct gctattcaga tttcatctaa 4320 ctgcgggaga ggggttcaaa ggggcgtgac gtcacagaca acgggtgaag gacgaagatt 4380 gcctcacttc tttgctagca atttgcctgc aaagaaggcg cacatgacaa gcaaacaact 4440 gggaaaccac tattgaatac ccacaatgca aagctcggaa ggtacgtctt gattgcagtg 4500 tgtcgagtgt caaaaaagaa gcaagtgttc atgcaagcca aaattggcac ctcctccact 4560 tctccgagtg ccccacccga acctccaggc gagatggccg gaacatacca tccgcgttgg 4620 gattatgacg tatctccttc ttcttcacat gattccatcc cgttgttgct tgtttgcgag 4680 ctgtgacggg agatcgtaga tgccacttcg ggccaggcag gcagtgcagg cagccaggaa 4740 cacaagcttc caacttggtc atctcgattg ccgattaagg gaaccaaatg cctatataag 4800 actgtcctcc cacctcccca ataccattct tttcttcttc catcatcagc caacaaagca 4860 atcacatctt cactacttca aatcaacaca acactcaaac cactttcaca acccctcaca 4920 tcaaccaaag gatccatgga acaaaagttg atttctgaag aagatttgaa cggtgaacaa 4980 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatcaatgg cggcggaggc 5040 ggcggaggcg gcgcgccatt aattaactag ttctagagcg gccgccaccg cggtggagct 5100 ccaattcgcc ctatagtgag tcgtattaag ggcgtggacg gctaatgggg tctgaatgct 5160 aaaagacacc atttcccaca ctccctctga ttttttttat ggtttttttt tatcttcatt 5220 tttcgccgtc ttttattcgg ttgtttggga ggttcacatg caacgtgcaa gactgcaagt 5280 tttttgagat gggcgcttca tgcaatgcag catcactgca gggacattgt gacatccacg 5340 tttcgatcgt ctgtgcttcc cccgttcatg tacgcatgat ctgaaaaaaa aaaaaaaaaa 5400 aaaggttgat gtaataatga aaataaacac ccagggacga gcacctcatc tgctggaata 5460 gggagaaaaa ctcaagggcc gtttgggtct ccacacttac caacagattc atgtactgat 5520 cttcagcctg tacagaacag tacaatgtcc aaaactcgtt atcattactc tatgtgccca 5580 aaatgaccca gatatacatc aacccactac atccttttgt tcttgttttt tttttttttt 5640 tttgctactt cgaaaagaat ttccgtttag ttgctcttgc tcatgtgctc aagacggatg 5700 ctgaccgccc ttctgtgagc ctcgagctcc tcaaccttag ccaactgcat gacagcctgg 5760 ccgacgtttt tgagaccctc ggcagtcaag ttggagctgg taatgtgctt gacgaacgag 5820 gcgaaattga cgccagagta ctgcttgcca aagccatagg tagctatgtg tcttgacgtt 5880 agcagaagaa tctgtgaagc ggggctattt atgatatgta acttacgcag cgagtggtta 5940 acaccagcag agtaatcgcc aacggactca ggagtccagg cgccaatgaa gacactaccg 6000 gcgttcatga caagatcgac agccttctcg gcctccttga tctggaggat caagtgctca 6060 ggagcgtact tgttgctgag ctccatggcc tcctcgacgg tcttgacctg cacggtgatc 6120 gagtgggcga tggagccacg gacaatctgg acgcgaggaa gctccgtagc ctgacggtga 6180 acctcgtcct caatagcctg aagatgctcc tcgtcgaggt caatagcgat caggatgacc 6240 tgactgtcaa cgccgtgctc agcctgggac aggagatccg aggcaacgaa cgcggggttg 6300 gcgtccttgt cagcgatgac cagcacctcg gacgggccag cgggcatgtc aataccaacg 6360 gcagcgttgg tgtcgttgct gacgaacatc ttggcagcag tgacgaactg gttaccgggg 6420 ccgagaatct tgtcgacctt ggtgatgctc tcggtgccgt aggccatggc agctacggcc 6480 tgggcaccgc cggcaagcac gatggactcg gccccaacct tgtgagcgac gtggacaatc 6540 tcgggagtga tggttccgtc ggcgcgggga ggagaggcga acacaatctt gttgcagccg 6600 gcgaccatgg cgggaacacc cagcataagg gcagtgctgg ggagaacggc ggtaccgccg 6660 gggatgtagc agccgacggc ctcgatggga cgagagaaac ggctgcagac aacaccgggc 6720 atggtctcga cctggagggg cttctcctcc ttctgggcgg cgtggaactt gcggatgttc 6780 tcgaaggaca cgtcgatggc agcaatggtc tcctcaggga gctgcataag ctccttgggg 6840 aagggcgcct tcaggacggg gctagtaaga gaggtagcct tctcgaactt gtgagtgtac 6900 gacagaacag ccttgtcgcc gttcttgcgg acgtcctcga tgatggggac aatgatcttg 6960 tagatggcat cggacgactt ttgcgcagga cgcttgagag cagcatcgag ctcctcggta 7020 gagaccttgg aggcgtcgaa acgtctcatg gtgatcttct caggggtggt ctcctgggcg 7080 gcctccttgg tgacaggggc cgaagtggca gcgagagcgg acgccgcagg cttgatgccc 7140 tccttctcag cccacttacc cttagcatct ccagtcctgc gcttgacctt ccagctcttg 7200 gcgtcaaggc tcctttcgat atcggcaaga gtaacgccgg cagcaacggc cctggtaaga 7260 gcaaagtaga agagatcggc agcctcaaag gcgatttcct ggggggtctg agcggtgcag 7320 agctcctcag cctcctccat gatcttggcc cggactagct tctcatcgga gaagagacgg 7380 gcagtgtagg agccctcggg ggcagactgt ttcctcgaaa tcaaagtctg ctcgagcttg 7440 ggaaggcctt tgagctgacc aaagcagccg gactgatcga ggtggcagaa acgacccttc 7500 tgcttcacga caaacttgag agcatcgtta tcgcagtcaa gcgagatgcg gacgagctcc 7560 tgagtgtctc cggaagtagc acccttgtac cagagaccgc gcttccggct ctgatagaca 7620 ccagtctgtg tcctgagggc ctcgttcaca ctctcggcac tgctgtagac cagacccaga 7680 gcagtgtcgt gctcatcaac gacaacggtg gggagaagac catcgggacg gtcagacttc 7740 cagacgctcg agaggatggt ggagatggca agcttcagct gctcgagttc 7790 n pCCG::N-3xMyc"Times New Roman ONdeI ArialRnyoco OScaI Arialn!',,Z O+HpaI(2327),KpnI(2480),NheI(2669),ClaI(2831) Arial npy O+HpaI(3320),ScaI(3640),XhoI(3652),XhoI(3833) Arial8nIS\\ O͊PvuII(3881),KpnI(4044),ApaI(4050),XhoI(4055),SalI(4061),ClaI(4071),HindIII(4076),EcoRV(4084),EcoRI(4088),MluI(4197),SexAI(4274),NheI(4395) Arial*)n}LDJ1ll1 OqHindIII(4745),BamHI(4931),NcoI(4935),AscI(5052),PacI(5064),SpeI(5067),XbaI(5073),EagI(5080),NotI(5080),SacI(5101) Arialn7 O+PstI(5321),XhoI(5722),SacI(5729),ScaI(5840) ArialZnok O+HpaI(5940),SacI(6083),NcoI(6084),SalI(6433) Arialn=;9q!Q O8NcoI(6465),NcoI(6606),KpnI(6656),PstI(6708),Bsu36I(6814) Arialn O,SacI(7013),EcoRV(7221),SacI(7325),XhoI(7432) Arial5n51,Z, OGSacI(7559),Bsu36I(7634),XhoI(7748),HindIII(7771),PvuII(7779),XhoI(7783) ArialDnq_m_OCɆn Sp6_primer"Times New RomanOn pGEX_3_primer"Times New RomanOCn AmpR_promoter"Times New RomanOCn0 Ampicillin"Times New RomanOCn0ORF 1"Times New RomanOCɆn6 pBR322_origin"Times New RomanOCn) ;  T7_promoter"Times New RomanOCn T3_promoter"Times New RomanOCnpBluescriptKS_primer"Times New RomanOn T7_promoter"Times New RomanOCɆn#ORF 1"Times New RomanOCn<ORF 2"Times New Roman Otest"Times New RomanAnw[lwh