Sequence of pCCG_N-FLAG

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Sequence of pCCG_N-FLAG_HAT

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Sequence of pCCG::N-FLAG::HAT: 7793 BP; 1934 A; 2054 C; 2005 G; 1800 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 60

1 atatcacagt ggatttagca tacacatact atgtattcca atacataatt aacatcggcg 60

Sp6_primer .......... .......... .......... .......... ..........

.......... .......... NdeI(82) .......... .......... ..........

61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat 120

61 caagattgct gttatacagg tataccacgt gagagtcatg ttagacgaga ctacggcgta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 180

121 tcaattcggt cggggctgtg ggcggttgtg ggcgactgcg cgggactgcc cgaacagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 240

181 agggccgtag gcgaatgtct gttcgacact ggcagaggcc ctcgacgtac acagtctcca 240

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 300

241 aaagtggcag tagtggcttt gcgcgctctg ctttcccgga gcactatgcg gataaaaata 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 360

301 tccaattaca gtactattat taccaaagaa tctgcagtcc accgtgaaaa gcccctttac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 420

361 acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata ggcgagtact 420

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 480

421 ctgttattgg gactatttac gaagttatta taactttttc cttctcatac tcataagttg 480

.......... .......... .......... .......... ....... Ampicillin ORF 1

.......... .......... .......... .......... .......... ..........

481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 540

481 taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg 540

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 600

541 gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt 600

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 660

601 agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag 660

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 720

661 gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca taactgcggc 720

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ScaI(775)

721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 780

721 ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg 780

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 840

781 gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt 840

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 900

841 attggtactc actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc 900

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 960

901 tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg 960

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1020

961 gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga catcgttacc 1020

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1080

1021 gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta 1080

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1140

1081 attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc 1140

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1200

1141 gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac 1200

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1260

1201 gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag 1260

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1320

1261 tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg 1320

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1380

1321 taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1440

1381 aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1500

1441 ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa 1500

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1560

1501 ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1620

1561 gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1680

1621 cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1740

1681 tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1800

1741 ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1860

1801 gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 1920

1861 tgtggcttga ctctatggat gtcgcactcg taactctttc gcggtgcgaa gggcttccct 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1980

1921 ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2040

1981 aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2100

2041 tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2160

2101 gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2220

2161 ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2280

2221 cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcgggttatg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg 2340

2281 cgtttggcgg agaggggcgc gcaaccggct aagtaattac gtccaattgg accgaatagc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact 2400

2341 tttaattatg ctgagtgata tccctctggc cgtctaggtt acgcctacct aagcgagtga 2400

......... T7_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt 2460

2401 gccacggcgc cgccacgctt caaggacggc acctaacccc ctaacgtgtc tgccacctca 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta 2520

2461 cgaacgatgt cgatccatgg gtcaacgtgt ggtttgccac ggggacacgg ggacgcagat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat 2580

2521 ccacacagga cactcgttga cgcgggcgcg cgccgtggcc cttcgacgcc cttggagtta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc 2640

2581 cctactaacc gcggtctagg ggccgtagtg gagtcgtgta cccgtcgggc tcttccttag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac 2700

2641 ctcccatctt ctataaggtc ccaaaggcga tcgtgttgaa ggttcgcgga gatggcgatg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta 2760

2701 aggtgtgtgg gtgcgactta ccgtgttggt gacatccgga tcgccagggt cgaggggcat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac 2820

2761 tggtgcgtcg gggtcgctgg ctccagtagt actgtgatac tgctgttaga ccgccctctg 2820

.......... .......... .......... .......... .......... ..........

.......... ClaI(2831) .......... .......... .......... ..........

2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc 2880

2821 aaccctttta gctactgccc gtggatggaa cccttgttat ctcgttcagg tcagttgcgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta 2940

2881 gatcccttcc cccttttttt ttttctcggg taagagtgtt cctcgttgtg acacgacgat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga 3000

2941 agtatcatgt gatgttcgtt tcagccctac aacgatctta ggcagccgat gcagccatct 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg 3060

3001 atggagggta tccgtcattc agatccatag gttaaatatc aaggcgcata aagaagcagc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc 3120

3061 ttgccccgtg tcgtttcgga ttgtcggcgt cacggagcgt tggttgtggt tcgtcattag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc 3180

3121 agtaggtagg tatgtctagg agggtgttat agaaatgtac gcgtgaacgc gacacaatcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg 3240

3181 tcaaatccaa ggtgatggtt ccgactgtgc tcgtcgtgac tgtcgtttga cgacgaaccc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa 3300

3241 cggttcttta accgtcaaac gactcgtgga tgtatagtca tgtgtagttg ggaccaaatt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac 3360

3301 aacaatcgga ccatgacaat tggcagtact tgctcctgca cctccttctt ggtgcgattg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat 3420

3361 ttaacttggt cccaactaca tatggagaga atggtataaa cgaacgaagg tgcagggata 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc 3480

3421 ggagttcgct aaaacaagaa ctgaaaaaca taaagttgcg tttccggtaa gtccaaagag 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac 3540

3481 tacaagttgg gaaacctacc gctacggcaa gcagggtcct ggagcactgg aaactttctg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa 3600

3541 ctctttatat agggtttcgt tgaagacgaa tacctgttcg ttgacgtacc gccgggtgtt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ScaI(3640) .......... ..........

3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac 3660

3601 gagtatagag cgcattagta ggtttgcgat ccgggctcat gagatgacaa gagctctgtg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg 3720

3661 gatggtatta tacatgggtt agtctgtact gttgtcagtt ggtttgtatg ggactaggcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg 3780

3721 ggtttgggac tgtagggatc tactaccaat ccgtgtgttt accttttgcc ctgagcaacc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... XhoI(3833)

3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc 3840

3781 aatccaccct tgcgaacata acgtcggtcg gttggctgag aggagcaggg agagctcagg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta 3900

3841 gcaataacgg caaactgggt cggcgggtcg gcggtttgtc gactccctcg gttaccagat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct 3960

3901 tatgtggcag gttgccgggg tcactacaaa gtttgaacta actgtcgctt gctttgggga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac 4020

3961 ctttgggcaa tatctataca tatctaaact gccccatcga accgggatta attgggagtg 4020

.......... .......... .......... .......... ......... T3_promoter

.......... .... KpnI(4044) ApaI(4050),XhoI(4055) SalI(4061) ....

4021 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt 4080

4021 atttcccttg ttttcgaccc atggcccggg ggggagctcc agctgccata gctattcgaa 4080

.......... .......... .......... ..... pBluescriptKS_primer .....

.......... .......... .......... .......... .......... ..........

4081 gatatcgaat tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg 4140

4081 ctatagctta agcaagtttc ggtgtagtga cccgtgaagg taaccctgtc cgtaactagc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gacgagaccg acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg 4200

4141 ctgctctggc tgaagaccgg cgaaagttgt cggtgtaata taggtacagt gccgatgcgc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tcggccttcg gtaacagaaa agcacacaga cagcgattgt gacatggatt cgggcaaagc 4260

4201 agccggaagc cattgtcttt tcgtgtgtct gtcgctaaca ctgtacctaa gcccgtttcg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 attggtggtc gcaccaggtc atcctgagtg tgcagtggct gctattcaga tttcatctaa 4320

4261 taaccaccag cgtggtccag taggactcac acgtcaccga cgataagtct aaagtagatt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctgcgggaga ggggttcaaa ggggcgtgac gtcacagaca acgggtgaag gacgaagatt 4380

4321 gacgccctct ccccaagttt ccccgcactg cagtgtctgt tgcccacttc ctgcttctaa 4380

.......... .......... .......... .......... .......... ..........

.......... NheI(4395) .......... .......... .......... ..........

4381 gcctcacttc tttgctagca atttgcctgc aaagaaggcg cacatgacaa gcaaacaact 4440

4381 cggagtgaag aaacgatcgt taaacggacg tttcttccgc gtgtactgtt cgtttgttga 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gggaaaccac tattgaatac ccacaatgca aagctcggaa ggtacgtctt gattgcagtg 4500

4441 ccctttggtg ataacttatg ggtgttacgt ttcgagcctt ccatgcagaa ctaacgtcac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgtcgagtgt caaaaaagaa gcaagtgttc atgcaagcca aaattggcac ctcctccact 4560

4501 acagctcaca gttttttctt cgttcacaag tacgttcggt tttaaccgtg gaggaggtga 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tctccgagtg ccccacccga acctccaggc gagatggccg gaacatacca tccgcgttgg 4620

4561 agaggctcac ggggtgggct tggaggtccg ctctaccggc cttgtatggt aggcgcaacc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gattatgacg tatctccttc ttcttcacat gattccatcc cgttgttgct tgtttgcgag 4680

4621 ctaatactgc atagaggaag aagaagtgta ctaaggtagg gcaacaacga acaaacgctc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctgtgacggg agatcgtaga tgccacttcg ggccaggcag gcagtgcagg cagccaggaa 4740

4681 gacactgccc tctagcatct acggtgaagc ccggtccgtc cgtcacgtcc gtcggtcctt 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cacaagcttc caacttggtc atctcgattg ccgattaagg gaaccaaatg cctatataag 4800

4741 gtgttcgaag gttgaaccag tagagctaac ggctaattcc cttggtttac ggatatattc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 actgtcctcc cacctcccca ataccattct tttcttcttc catcatcagc caacaaagca 4860

4801 tgacaggagg gtggaggggt tatggtaaga aaagaagaag gtagtagtcg gttgtttcgt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atcacatctt cactacttca aatcaacaca acactcaaac cactttcaca acccctcaca 4920

4861 tagtgtagaa gtgatgaagt ttagttgtgt tgtgagtttg gtgaaagtgt tggggagtgt 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcaaccaaag gatccatgga ctacaaagac gatgacgaca agggcggagg cttaatcggc 4980

4921 agttggtttc ctaggtacct gatgtttctg ctactgctgt tcccgcctcc gaattagccg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ttgaaggatc atctcatcca caatgtccac aaagaggagc acgctcatgc ccacaacaag 5040

4981 aacttcctag tagagtaggt gttacaggtg tttctcctcg tgcgagtacg ggtgttgttc 5040

.......... .......... .......... .......... HAT_primer ..........

.... AscI(5055) PacI(5067),SpeI(5070) XbaI(5076) .... ..........

5041 ggcggcggag gcggcgcgcc attaattaac tagttctaga gcggccgcca ccgcggtgga 5100

5041 ccgccgcctc cgccgcgcgg taattaattg atcaagatct cgccggcggt ggcgccacct 5100

.......... .......... .......... .......... .......... ..........

SacI(5104) .......... .......... .......... .......... ..........

5101 gctccaattc gccctatagt gagtcgtatt aagggcgtgg acggctaatg gggtctgaat 5160

5101 cgaggttaag cgggatatca ctcagcataa ttcccgcacc tgccgattac cccagactta 5160

.......... ......... T7_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gctaaaagac accatttccc acactccctc tgattttttt tatggttttt ttttatcttc 5220

5161 cgattttctg tggtaaaggg tgtgagggag actaaaaaaa ataccaaaaa aaaatagaag 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atttttcgcc gtcttttatt cggttgtttg ggaggttcac atgcaacgtg caagactgca 5280

5221 taaaaagcgg cagaaaataa gccaacaaac cctccaagtg tacgttgcac gttctgacgt 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agttttttga gatgggcgct tcatgcaatg cagcatcact gcagggacat tgtgacatcc 5340

5281 tcaaaaaact ctacccgcga agtacgttac gtcgtagtga cgtccctgta acactgtagg 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 acgtttcgat cgtctgtgct tcccccgttc atgtacgcat gatctgaaaa aaaaaaaaaa 5400

5341 tgcaaagcta gcagacacga agggggcaag tacatgcgta ctagactttt tttttttttt 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaaaaaggtt gatgtaataa tgaaaataaa cacccaggga cgagcacctc atctgctgga 5460

5401 ttttttccaa ctacattatt acttttattt gtgggtccct gctcgtggag tagacgacct 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 atagggagaa aaactcaagg gccgtttggg tctccacact taccaacaga ttcatgtact 5520

5461 tatccctctt tttgagttcc cggcaaaccc agaggtgtga atggttgtct aagtacatga 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gatcttcagc ctgtacagaa cagtacaatg tccaaaactc gttatcatta ctctatgtgc 5580

5521 ctagaagtcg gacatgtctt gtcatgttac aggttttgag caatagtaat gagatacacg 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccaaaatgac ccagatatac atcaacccac tacatccttt tgttcttgtt tttttttttt 5640

5581 ggttttactg ggtctatatg tagttgggtg atgtaggaaa acaagaacaa aaaaaaaaaa 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ttttttgcta cttcgaaaag aatttccgtt tagttgctct tgctcatgtg ctcaagacgg 5700

5641 aaaaaacgat gaagcttttc ttaaaggcaa atcaacgaga acgagtacac gagttctgcc 5700

.......... .......... .......... .......... ORF 1 ..........

.......... .......... XhoI(5725) .......... .......... ..........

5701 atgctgaccg cccttctgtg agcctcgagc tcctcaacct tagccaactg catgacagcc 5760

5701 tacgactggc gggaagacac tcggagctcg aggagttgga atcggttgac gtactgtcgg 5760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tggccgacgt ttttgagacc ctcggcagtc aagttggagc tggtaatgtg cttgacgaac 5820

5761 accggctgca aaaactctgg gagccgtcag ttcaacctcg accattacac gaactgcttg 5820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... ScaI(5843) .......... .......... ..........

5821 gaggcgaaat tgacgccaga gtactgcttg ccaaagccat aggtagctat gtgtcttgac 5880

5821 ctccgcttta actgcggtct catgacgaac ggtttcggta tccatcgata cacagaactg 5880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gttagcagaa gaatctgtga agcggggcta tttatgatat gtaacttacg cagcgagtgg 5940

5881 caatcgtctt cttagacact tcgccccgat aaatactata cattgaatgc gtcgctcacc 5940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ttaacaccag cagagtaatc gccaacggac tcaggagtcc aggcgccaat gaagacacta 6000

5941 aattgtggtc gtctcattag cggttgcctg agtcctcagg tccgcggtta cttctgtgat 6000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ccggcgttca tgacaagatc gacagccttc tcggcctcct tgatctggag gatcaagtgc 6060

6001 ggccgcaagt actgttctag ctgtcggaag agccggagga actagacctc ctagttcacg 6060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tcaggagcgt acttgttgct gagctccatg gcctcctcga cggtcttgac ctgcacggtg 6120

6061 agtcctcgca tgaacaacga ctcgaggtac cggaggagct gccagaactg gacgtgccac 6120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 atcgagtggg cgatggagcc acggacaatc tggacgcgag gaagctccgt agcctgacgg 6180

6121 tagctcaccc gctacctcgg tgcctgttag acctgcgctc cttcgaggca tcggactgcc 6180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tgaacctcgt cctcaatagc ctgaagatgc tcctcgtcga ggtcaatagc gatcaggatg 6240

6181 acttggagca ggagttatcg gacttctacg aggagcagct ccagttatcg ctagtcctac 6240

.......... .......... ORF 1 .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

6241 acctgactgt caacgccgtg ctcagcctgg gacaggagat ccgaggcaac gaacgcgggg 6300

6241 tggactgaca gttgcggcac gagtcggacc ctgtcctcta ggctccgttg cttgcgcccc 6300

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ttggcgtcct tgtcagcgat gaccagcacc tcggacgggc cagcgggcat gtcaatacca 6360

6301 aaccgcagga acagtcgcta ctggtcgtgg agcctgcccg gtcgcccgta cagttatggt 6360

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 acggcagcgt tggtgtcgtt gctgacgaac atcttggcag cagtgacgaa ctggttaccg 6420

6361 tgccgtcgca accacagcaa cgactgcttg tagaaccgtc gtcactgctt gaccaatggc 6420

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... SalI(6436) .......... .......... .......... ..........

6421 gggccgagaa tcttgtcgac cttggtgatg ctctcggtgc cgtaggccat ggcagctacg 6480

6421 cccggctctt agaacagctg gaaccactac gagagccacg gcatccggta ccgtcgatgc 6480

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gcctgggcac cgccggcaag cacgatggac tcggccccaa ccttgtgagc gacgtggaca 6540

6481 cggacccgtg gcggccgttc gtgctacctg agccggggtt ggaacactcg ctgcacctgt 6540

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 atctcgggag tgatggttcc gtcggcgcgg ggaggagagg cgaacacaat cttgttgcag 6600

6541 tagagccctc actaccaagg cagccgcgcc cctcctctcc gcttgtgtta gaacaacgtc 6600

.......... ......... ORF 1 ORF 2 ......... .......... ..........

NcoI(6609) .......... .......... .......... .......... ..........

6601 ccggcgacca tggcgggaac acccagcata agggcagtgc tggggagaac ggcggtaccg 6660

6601 ggccgctggt accgcccttg tgggtcgtat tcccgtcacg acccctcttg ccgccatggc 6660

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ccggggatgt agcagccgac ggcctcgatg ggacgagaga aacggctgca gacaacaccg 6720

6661 ggcccctaca tcgtcggctg ccggagctac cctgctctct ttgccgacgt ctgttgtggc 6720

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ggcatggtct cgacctggag gggcttctcc tccttctggg cggcgtggaa cttgcggatg 6780

6721 ccgtaccaga gctggacctc cccgaagagg aggaagaccc gccgcacctt gaacgcctac 6780

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... ......... Bsu36I(6817) ......... ..........

6781 ttctcgaagg acacgtcgat ggcagcaatg gtctcctcag ggagctgcat aagctccttg 6840

6781 aagagcttcc tgtgcagcta ccgtcgttac cagaggagtc cctcgacgta ttcgaggaac 6840

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gggaagggcg ccttcaggac ggggctagta agagaggtag ccttctcgaa cttgtgagtg 6900

6841 cccttcccgc ggaagtcctg ccccgatcat tctctccatc ggaagagctt gaacactcac 6900

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tacgacagaa cagccttgtc gccgttcttg cggacgtcct cgatgatggg gacaatgatc 6960

6901 atgctgtctt gtcggaacag cggcaagaac gcctgcagga gctactaccc ctgttactag 6960

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ttgtagatgg catcggacga cttttgcgca ggacgcttga gagcagcatc gagctcctcg 7020

6961 aacatctacc gtagcctgct gaaaacgcgt cctgcgaact ctcgtcgtag ctcgaggagc 7020

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gtagagacct tggaggcgtc gaaacgtctc atggtgatct tctcaggggt ggtctcctgg 7080

7021 catctctgga acctccgcag ctttgcagag taccactaga agagtcccca ccagaggacc 7080

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gcggcctcct tggtgacagg ggccgaagtg gcagcgagag cggacgccgc aggcttgatg 7140

7081 cgccggagga accactgtcc ccggcttcac cgtcgctctc gcctgcggcg tccgaactac 7140

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ccctccttct cagcccactt acccttagca tctccagtcc tgcgcttgac cttccagctc 7200

7141 gggaggaaga gtcgggtgaa tgggaatcgt agaggtcagg acgcgaactg gaaggtcgag 7200

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ttggcgtcaa ggctcctttc gatatcggca agagtaacgc cggcagcaac ggccctggta 7260

7201 aaccgcagtt ccgaggaaag ctatagccgt tctcattgcg gccgtcgttg ccgggaccat 7260

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 agagcaaagt agaagagatc ggcagcctca aaggcgattt cctggggggt ctgagcggtg 7320

7261 tctcgtttca tcttctctag ccgtcggagt ttccgctaaa ggacccccca gactcgccac 7320

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 cagagctcct cagcctcctc catgatcttg gcccggacta gcttctcatc ggagaagaga 7380

7321 gtctcgagga gtcggaggag gtactagaac cgggcctgat cgaagagtag cctcttctct 7380

.......... ORF 1 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... XhoI(7435)

7381 cgggcagtgt aggagccctc gggggcagac tgtttcctcg aaatcaaagt ctgctcgagc 7440

7381 gcccgtcaca tcctcgggag cccccgtctg acaaaggagc tttagtttca gacgagctcg 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 ttgggaaggc ctttgagctg accaaagcag ccggactgat cgaggtggca gaaacgaccc 7500

7441 aacccttccg gaaactcgac tggtttcgtc ggcctgacta gctccaccgt ctttgctggg 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 ttctgcttca cgacaaactt gagagcatcg ttatcgcagt caagcgagat gcggacgagc 7560

7501 aagacgaagt gctgtttgaa ctctcgtagc aatagcgtca gttcgctcta cgcctgctcg 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tcctgagtgt ctccggaagt agcacccttg taccagagac cgcgcttccg gctctgatag 7620

7561 aggactcaca gaggccttca tcgtgggaac atggtctctg gcgcgaaggc cgagactatc 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 acaccagtct gtgtcctgag ggcctcgttc acactctcgg cactgctgta gaccagaccc 7680

7621 tgtggtcaga cacaggactc ccggagcaag tgtgagagcc gtgacgacat ctggtctggg 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 agagcagtgt cgtgctcatc aacgacaacg gtggggagaa gaccatcggg acggtcagac 7740

7681 tctcgtcaca gcacgagtag ttgctgttgc cacccctctt ctggtagccc tgccagtctg 7740

.......... .......... .......... .......... .......... ..........

.......... XhoI(7751) .. HindIII(7774) PvuII(7782),XhoI(7786) ..

7741 ttccagacgc tcgagaggat ggtggagatg gcaagcttca gctgctcgag ttc....... 7793

7741 aaggtctgcg agctctccta ccacctctac cgttcgaagt cgacgagctc aag....... 7793

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-7793 Reference author: Honda S., Selker E.U.; Reference title: "Tools for fungal proteomics: Multifunctional Neurospora vectors for gene Reference title: replacement, protein expression and protein purification"; Reference location: Genetics 0:0-0(2009).
Reference number: [2] Reference positions: 1-7793 Reference author: Honda S., Selker E.U.; Reference title: ; Reference location: Submitted (11-NOV-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA