cc&>pCFJ150 - pDESTttTi5605[R4-R3] plasmid sequence 9131 bp tgtgaaattgttatccgctggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcagga aagaacatgtgagcaaaaggccagcaaaagaccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccg cccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgt ttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcg ggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgt gcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgact tatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtgg tggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagt tggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaa aaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggatt ttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat atatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcat ccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgata ccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcc tgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgc gcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaa cgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaag taagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgct tttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtca atacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctc aaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttca ccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaata ctcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtat ttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatca tgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctga cacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagc gggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtg aaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaaggg cgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgcc agggttttcccagtcacgacgtCACGTGtgtttttgaacacggcgatatgtatctgtagatcatccccaattttcaacaa tgaatctaaatactgaactttttgtgtaatctttagtgcagaaaaatgaccaaaatcagcgattttccgattatacccaa aatatctcagctcaatgagcacctataaaaatgtaaaaataaagaaagtagtcgaaccatattttgtaaaggctaaaaaa ctgaataaatttttatccatccagcgcggcgttacaatccgtggcccatgtcacgcacggctttcaaagaaaaaaaaaca gtctgtttgcgcgacatcgatctggcaaaaatcctaaaaacgactatgcgtggctcactttatattaatatgaaaaatac aaacttggtggtgcaggtgctgcagaggccatcgatgaagaagtggaggccttgttgtcgatctacaaaattgtatggtt gtttgagattttccttgaaacgctctcaatcttggatatgatataaattctggataagatatgatggatatgatctatgt ttgatagagtgcatttgaaaggataacggccctgcagacatttttgaaaaaataacaaacatttaattttctcactcgtt taggctattcccctatttttgatattccttcgcacatatgaaaactactttttttcgaaactgttaactccagaatttat aaatctatagcccttacttgattatttattatcatggttactcacctcatgtctctttctcttttcaagaagtcttcgtt catatctgttgtctctttcctccttttgaatctctgctttgctcttcgccattgtttcctgaaaataatgtaacttgaat tgtgtaatatacttttttaatttgaatttggcttgtaacgcggaatcactacgtgcgggatcatttcttactagaaaacc cagaaaatgccatatttcacttatctcggggtcatttctaattagaaaagctacaaaaaccatttctaattacggtgact ttagaactggtcgaaatagctaattagaaatgggtttttgctaattagaggtgactctatgttatctgagtaagcctcgt ttgtatgtaaactcacagtactaataaatgcaagacacccgggtttgtctagatatgaaataattgaaatatcaattctg acagacaataatggtaatcttgataaggagttccacgcccaggagaacacgttagttttcttgtttttgattgcgtgcgt tattttggagaaaaactcgattttttacaaaataattttttgaaaggaacactgttcaataagttttgtcttttttctca gttgtgatacggttttttattcttttttgtagttatacagaagaccgttacgaaacagactgatatcgaaacaaacactg atcttacttgcacttaTAATACGACTCACTAGTGGGCAGATCTTCGAATGCATCGCGCGCACCGTACGTCTCGAGtgtaa aacgacggccagtgaattatcaactatgtataataaagttgaacgagaaacgtaaaatgatataaatatcaatatattaa attagattttgcataaaaaacagactacataatactgtaaaacacaacatatccagtcactatggcggccgcattaggca cccccaggctttacactttatgcttccggctcgtataatgtgtggattttgagttaggatccgtcgagattttcaggagc taaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttg aggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaag aaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaattccgtatggc aatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgtttt catcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaa aacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttt tgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaagg tgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaa cagtactgcgatgagtggcagggcggggcgtaatctagaggatccggcttactaaaagccagataacagtatgcgtattt gcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagca gcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggttcggtaag cacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcg cccggtttattgaaatgaacggctcttttgccgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataa aagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccc tggccagtgcacgtctgctgtcagataaagtcccccgtgaactttacccggtggtgcatatcggggatgaaagctggcgc atgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatga catcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagccagtctgcaggtcgac catagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatt tatatcattttacgtttctcgttcaacttttctatacaaagttgatagcttggcgtaatcatggtcatagctgttcctgC CTAGGTGAGCTCTGGTACCCTCTAGTCAAGGCCTTAAGTGAGTCGTATTACGTAGCCCGGGCctagttctagacattctc taatgaaaaaatctttcagttgaaattgaaaatgagttaaagttggagtttttattgaaaacagatttccgtgtgattag tgtttttagcgagtgtgacaggacagcgaaaaaatatagaaacaaggggggaactgaaaagcttaggaatgcattgaaca tgagaaggggaaggggaaggaacaaactagacaggaattattggaatttaatcacatttggagttttttttctattcgac agaataattatccagaacatttttgtattaaatatttatgcatcatatgagtagtcggctttgttgtgcatgacgagttt gttatcgacgaaatagaagctgtcagaacgagtctcgtttggattgttgatcatgtcgtccactgaaaaagagattagtc ttttgaattgtactttttagaataatgactcactgagttgttgagagagttgagggaactcatagatatgttcacagttg tttcgtgaattcggaatacagaatccgaattcaaagtcaaaacacttcagaaggcgatctttgaagaagtgacgttcgat cattcggaaatgatggattgggatgtctcctactttgaattccacagttgctccgaccgttttgagtttcaaaaagtttg gagcaaatctataacgcacgtatcttgccgactcctgtggcgactcatcattctcctgatcgttctccggtttggcgatc tcgaaaagcacttgctcagtgtccagatcacggatttggaacttggtgaactcgatgttatagatgttcgcagatgggga gcataagaatcctaaatttatgttttaaactgaaatccaaagggagcaagataccttgagtgattcccggaagtgctaaa acgtcgttcggagtgatttgagctttcttcgcaagctccgattccgttgtgattccttgttcggtgcttggtggtggccg tggcatctggaaatatggaaaagttcaacaaaaagaaaagagaaaagaatgaaatcggatatcaagagttagttgagcgg tttctctagttttctgagtctcacctgcgacgggaaggtcgccgagccgggtggaatcgatcgttgttgctcggctttca tatcggtttggttggaagcggctgaaaacggaaagaagtggaagaaggaaaagagtgtggtgtgacaggaaaatggtaat tagagggtgccaaataaccagctatattttgtttttttttgaaaacatttttaaaaagaaaaatacgataatgatatcag atggatttccggaaaactggtatgaaaaatttcaacctttttgagtacatgtaatcaaaatacactttgtaaattatcat ttttattgaaactccaccatttttctatttataacgctaataatttgaaaaagaaacctAttgcgaaccgcggggtgaat cccaaaaacgaatgcgttttggtggagtgattgattcgaatcgaagaagaaaaagaagaagacgtggaatagagagctca ctcttaaccgagcagcacacaccgacagaaaaaaaaatgaaatgaatgagggtcttcttcttcttcttcttcgaatgatt gacagaaatgggaaaaagaggaagattgagaagggaaaaaggaaggagaaaagaagcagaagaagacgtcagagaggaga ggaacgagcggaaaagcagcgggcgcaagtcatagaagtagcagagctggggagaagaagacactatccaagaaaggaat gacgagagagtatgcaaaggggtatagggtgcagacagaataggaacagaataacagatgatgagccaagaagagttgaa aagggcgatgaatttgtcatgtaacttaatttgggtcaatttgagcatgatgaattgaaatcatcccttgttgggagtta ataaccggtttgttatcagaaaccctgtaatagaagggcgccctaactttgagccaattcatcccggtttctgtcaaata tatcaaaaagtggtcaactgacaaattgtttttgatattataataaacattttatccgttaacaattttcgaatactttt tacaaggacttggataaattggctcaaagacgaagtgagtttgctaccatcatggaatcaggaggacatttcgacaatgt catataatgtttgaaaaatgattttaacaagggtctcattcgagataagcaatgctctgagcaagtggctcgtcgtcttc aagaatctcttgttgatattcatttggatcctgttttgcacgaagcatgtgccatggatattcagagtttttgcaaaaaa gttccgccaggtcaaacaatctttttaatttttatatgatacttcgggtgtatttttcttctttttcctgttcattttcg cttttttctcacttttttgtaacaaaatctctcacttttctgtaagtcttaacaaaagttcaattggctcgatcaagtaa aatttcggccgtatgaacgttttcaaaggttttcatttttttttaagttcattcacaatatcatgaatctctcgtccttt ggtttccggtaagtagcgaaataatataagcatacttataaatgaaggaattgaatatagaataaggaaagcataactac caataacggaatatagaggtaatactgtaaatgtactgataaccaccattacagtattcattgaatatgccacagattga gcaatactacgatgtttttgaggtaccagttcagaggatataaaccacgaaattggaccaactccagttctgaaatttca ttattcgattaaatcaaaatatatttgaaaaacctcacccataagtataaccgtagatgatcaatgcaactaggcatccg tatttcatcgggtcaatcagaatacgtatttttgcgaagaatacgaatgcaattagagctaaattattgagaaatgtgaa gagaaggagaagcttgcgtcgaccaactctgtaaacttattcatacaattttgtcttcaaattttgaacgtttaaaacta tcttattcaatgagttagcgggtttttatagtgagttagttcacgattatctaataaaactatgtccaaacatcctaact gcgaccgtattctcactaactcgtcttaaaaacgttgagattattttaaacccttcttcagtacaactacagtactgatg cacaaatacaataataataatacattcagacatactctgaacaatgtaacttgtttttgttatactgtggtaacatttat tgaaatgaaatataaccttacttttcaatcacaattccaccagtaattgttccagaaacataagctaaacccattgcagt tgtactccattcagcaatttctgactcaacatttgcattttctagaaagaaagtagaggataaaagaagagcccataatc cgacaGGGCCC Help: BLASTN BLAST programs find regions of similarity between biological sequences. BLASTN searches NCBI's DNA database for DNA sequences similar to your sequence. Help: More Sequence Options View the sequence in different formats, find restriction sites, view open reading frame translations, and more. Helpful Tip: Click on the "Analyze Sequence" button to see restriction sites, view ORFs, etc. Addgene's Sequencing Results %pCFJ150 - pDESTttTi5605[R4-R3] "Times New RomancC# pBR322_origin"Times New RomancC#ORF 2"Times New Roman(cCɆ# Ampicillin"Times New Romanc#+AmpR_pro"Times New RomancC# pGEX_3_pri"Times New RomancC#M13_f20"Times New RomancCɆ#,tattR2"Times New Romanc#,attR1"Times New RomancC#lac_pro"Times New RomancC#ORF 2"Times New RomancCɆ#CAT/CamR"Times New Romanc#ccdB"Times New RomancC#AattR1"Times New RomancC#AattR2"Times New Roman cPmlI"Times New Roman) #7770> c#XhoI(3830), BglII(3798), SpeI(3788)"Times New Roman L#=GQQ. cNotI"Times New Roman#$+2Q  cAflII"Times New Roman #J=// cSacII"Times New Roman #    cAgeI"Times New Roman #  cApaI"Times New Roman##cO;,; ctest"Times New Romanr#  ~o