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    Plasmid map of pCFJ151

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    pCFJ151 - ttTi5605_MCS
    Gene/insert name: ttTi5605 targeting region
    Insert size (bp): 5075
    Species of gene(s): C. elegans (nematode)
    Vector backbone: N/A (Search Vector Database)
    Type of vector: Worm expression
    Backbone size (bp): 2334
    5' Sequencing primer: n/a (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: Erik Jorgensen
    Comments: Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
    Article: Single-copy insertion of transgenes in Caenorhabditis elegans Christian Frøkjær-Jensen et al. ( Pubmed)

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