Sequence of pCFJ151

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Sequence of pCFJ151

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Sequence of pCFJ151 - ttTi5605_MCS : 7409 BP; 2227 A; 1430 C; 1602 G; 2150 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 60

1 acactttaac aataggcgac catagtcgag tgagtttccg ccattatgcc aataggtgtc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc 120

61 ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc tggtccttgg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 180

121 catttttccg gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt 180

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 240

181 ttttagctgc gagttcagtc tccaccgctt tgggctgtcc tgatatttct atggtccgca 240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 300

241 aagggggacc ttcgagggag cacgcgagag gacaaggctg ggacggcgaa tggcctatgg 300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 360

301 acaggcggaa agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg acatccatag 360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 420

361 agtcaagcca catccagcaa gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg 420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 480

421 ggctggcgac gcggaatagg ccattgatag cagaactcag gttgggccat tctgtgctga 480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 540

481 atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata catccgccac 540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 600

541 gatgtctcaa gaacttcacc accggattga tgccgatgtg atcttcctgt cataaaccat 600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 660

601 agacgcgaga cgacttcggt caatggaagc ctttttctca accatcgaga actaggccgt 660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 720

661 ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt 720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 780

721 tttttcctag agttcttcta ggaaactaga aaagatgccc cagactgcga gtcaccttgc 780

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 840

781 ttttgagtgc aattccctaa aaccagtact ctaatagttt ttcctagaag tggatctagg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 900

841 aaaatttaat ttttacttca aaatttagtt agatttcata tatactcatt tgaaccagac 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 960

901 tgtcaatggt tacgaattag tcactccgtg gatagagtcg ctagacagat aaagcaagta 960

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 1020

961 ggtatcaacg gactgagggg cagcacatct attgatgcta tgccctcccg aatggtagac 1020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 1080

1021 cggggtcacg acgttactat ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt 1080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 1140

1081 atttggtcgg tcggccttcc cggctcgcgt cttcaccagg acgttgaaat aggcggaggt 1140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 1200

1141 aggtcagata attaacaacg gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg 1200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 1260

1201 cgttgcaaca acggtaacga tgtccgtagc accacagtgc gagcagcaaa ccataccgaa 1260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 1320

1261 gtaagtcgag gccaagggtt gctagttccg ctcaatgtac tagggggtac aacacgtttt 1320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 1380

1321 ttcgccaatc gaggaagcca ggaggctagc aacagtcttc attcaaccgg cgtcacaata 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 1440

1381 gtgagtacca ataccgtcgt gacgtattaa gagaatgaca gtacggtagg cattctacga 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 1500

1441 aaagacactg accactcatg agttggttca gtaagactct tatcacatac gccgctggct 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 1560

1501 caacgagaac gggccgcagt tatgccctat tatggcgcgg tgtatcgtct tgaaattttc 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 1620

1561 acgagtagta accttttgca agaagccccg cttttgagag ttcctagaat ggcgacaact 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 1680

1621 ctaggtcaag ctacattggg tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 1740

1681 ggtcgcaaag acccactcgt ttttgtcctt ccgttttacg gcgttttttc ccttattccc 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 1800

1741 gctgtgcctt tacaacttat gagtatgaga aggaaaaagt tataataact tcgtaaatag 1800

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 1860

1801 tcccaataac agagtactcg cctatgtata aacttacata aatcttttta tttgtttatc 1860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 1920

1861 cccaaggcgc gtgtaaaggg gcttttcacg gtggactgca gattctttgg taataatagt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 1980

1921 actgtaattg gatattttta tccgcatagt gctccgggaa agcagagcgc gcaaagccac 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 2040

1981 tactgccact tttggagact gtgtacgtcg agggcctctg ccagtgtcga acagacattc 2040

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 2100

2041 gcctacggcc ctcgtctgtt cgggcagtcc cgcgcagtcg cccacaaccg cccacagccc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 2160

2101 cgaccgaatt gatacgccgt agtctcgtct aacatgactc tcacgtggta tacgccacac 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 2220

2161 tttatggcgt gtctacgcat tcctctttta tggcgtagtc cgcggtaagc ggtaagtccg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 2280

2221 acgcgttgac aacccttccc gctagccacg cccggagaag cgataatgcg gtcgaccgct 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... PmlI(2325) ..........

2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agcacgtgtg tttttgaaca 2340

2281 ttccccctac acgacgttcc gctaattcaa cccattgcgg tcgtgcacac aaaaacttgt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cggcgatatg tatctgtaga tcatccccaa ttttcaacaa tgaatctaaa tactgaactt 2400

2341 gccgctatac atagacatct agtaggggtt aaaagttgtt acttagattt atgacttgaa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc gattttccga ttatacccaa 2460

2401 aaacacatta gaaatcacgt ctttttactg gttttagtcg ctaaaaggct aatatgggtt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aatatctcag ctcaatgagc acctataaaa atgtaaaaat aaagaaagta gtcgaaccat 2520

2461 ttatagagtc gagttactcg tggatatttt tacattttta tttctttcat cagcttggta 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 attttgtaaa ggctaaaaaa ctgaataaat ttttatccat ccagcgcggc gttacaatcc 2580

2521 taaaacattt ccgatttttt gacttattta aaaataggta ggtcgcgccg caatgttagg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca gtctgtttgc gcgacatcga 2640

2581 caccgggtac agtgcgtgcc gaaagtttct ttttttttgt cagacaaacg cgctgtagct 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tctggcaaaa atcctaaaaa cgactatgcg tggctcactt tatattaata tgaaaaatac 2700

2641 agaccgtttt taggattttt gctgatacgc accgagtgaa atataattat actttttatg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag aagtggaggc cttgttgtcg 2760

2701 tttgaaccac cacgtccacg acgtctccgg tagctacttc ttcacctccg gaacaacagc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa cgctctcaat cttggatatg 2820

2761 tagatgtttt aacataccaa caaactctaa aaggaacttt gcgagagtta gaacctatac 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atataaattc tggataagat atgatggata tgatctatgt ttgatagagt gcatttgaaa 2880

2821 tatatttaag acctattcta tactacctat actagataca aactatctca cgtaaacttt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggataacggc cctgcagaca tttttgaaaa aataacaaac atttaatttt ctcactcgtt 2940

2881 cctattgccg ggacgtctgt aaaaactttt ttattgtttg taaattaaaa gagtgagcaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taggctattc ccctattttt gatattcctt cgcacatatg aaaactactt tttttcgaaa 3000

2941 atccgataag gggataaaaa ctataaggaa gcgtgtatac ttttgatgaa aaaaagcttt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctgttaactc cagaatttat aaatctatag cccttacttg attatttatt atcatggtta 3060

3001 gacaattgag gtcttaaata tttagatatc gggaatgaac taataaataa tagtaccaat 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctcacctcat gtctctttct cttttcaaga agtcttcgtt catatctgtt gtctctttcc 3120

3061 gagtggagta cagagaaaga gaaaagttct tcagaagcaa gtatagacaa cagagaaagg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tccttttgaa tctctgcttt gctcttcgcc attgtttcct gaaaataatg taacttgaat 3180

3121 aggaaaactt agagacgaaa cgagaagcgg taacaaagga cttttattac attgaactta 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgtgtaatat acttttttaa tttgaatttg gcttgtaacg cggaatcact acgtgcggga 3240

3181 acacattata tgaaaaaatt aaacttaaac cgaacattgc gccttagtga tgcacgccct 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcatttctta ctagaaaacc cagaaaatgc catatttcac ttatctcggg gtcatttcta 3300

3241 agtaaagaat gatcttttgg gtcttttacg gtataaagtg aatagagccc cagtaaagat 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 attagaaaag ctacaaaaac catttctaat tacggtgact ttagaactgg tcgaaatagc 3360

3301 taatcttttc gatgtttttg gtaaagatta atgccactga aatcttgacc agctttatcg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 taattagaaa tgggtttttg ctaattagag gtgactctat gttatctgag taagcctcgt 3420

3361 attaatcttt acccaaaaac gattaatctc cactgagata caatagactc attcggagca 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttgtatgtaa actcacagta ctaataaatg caagacaccc gggtttgtct agatatgaaa 3480

3421 aacatacatt tgagtgtcat gattatttac gttctgtggg cccaaacaga tctatacttt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taattgaaat atcaattctg acagacaata atggtaatct tgataaggag ttccacgccc 3540

3481 attaacttta tagttaagac tgtctgttat taccattaga actattcctc aaggtgcggg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aggagaacac gttagttttc ttgtttttga ttgcgtgcgt tattttggag aaaaactcga 3600

3541 tcctcttgtg caatcaaaag aacaaaaact aacgcacgca ataaaacctc tttttgagct 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttttttacaa aataattttt tgaaaggaac actgttcaat aagttttgtc ttttttctca 3660

3601 aaaaaatgtt ttattaaaaa actttccttg tgacaagtta ttcaaaacag aaaaaagagt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttgtgatac ggttttttat tcttttttgt agttatacag aagaccgtta cgaaacagac 3720

3661 caacactatg ccaaaaaata agaaaaaaca tcaatatgtc ttctggcaat gctttgtctg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SpeI(3768) ..........

3721 tgatatcgaa acaaacactg atcttacttg cacttataat acgactcact agtgggcaga 3780

3721 actatagctt tgtttgtgac tagaatgaac gtgaatatta tgctgagtga tcacccgtct 3780

.......... .......... .......... .......... .......... ..........

.......... .......... XhoI(3810) .......... .......... ..........

3781 tcttcgaatg catcgcgcgc accgtacgtc tcgaggaatt cctgcaggat atctggatcc 3840

3781 agaagcttac gtagcgcgcg tggcatgcag agctccttaa ggacgtccta tagacctagg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 acgaagcttc ccatggtgac gtcaccggtt ctagatacct aggtgagctc tggtaccctc 3900

3841 tgcttcgaag ggtaccactg cagtggccaa gatctatgga tccactcgag accatgggag 3900

.......... .......... .......... .......... .......... ..........

......... AflII(3911) ......... .......... .......... ..........

3901 tagtcaaggc cttaagtgag tcgtattacg tagcccgggc ctagttctag acattctcta 3960

3901 atcagttccg gaattcactc agcataatgc atcgggcccg gatcaagatc tgtaagagat 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atgaaaaaat ctttcagttg aaattgaaaa tgagttaaag ttggagtttt tattgaaaac 4020

3961 tactttttta gaaagtcaac tttaactttt actcaatttc aacctcaaaa ataacttttg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agatttccgt gtgattagtg tttttagcga gtgtgacagg acagcgaaaa aatatagaaa 4080

4021 tctaaaggca cactaatcac aaaaatcgct cacactgtcc tgtcgctttt ttatatcttt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caagggggga actgaaaagc ttaggaatgc attgaacatg agaaggggaa ggggaaggaa 4140

4081 gttcccccct tgacttttcg aatccttacg taacttgtac tcttcccctt ccccttcctt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 caaactagac aggaattatt ggaatttaat cacatttgga gttttttttc tattcgacag 4200

4141 gtttgatctg tccttaataa ccttaaatta gtgtaaacct caaaaaaaag ataagctgtc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aataattatc cagaacattt ttgtattaaa tatttatgca tcatatgagt agtcggcttt 4260

4201 ttattaatag gtcttgtaaa aacataattt ataaatacgt agtatactca tcagccgaaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gttgtgcatg acgagtttgt tatcgacgaa atagaagctg tcagaacgag tctcgtttgg 4320

4261 caacacgtac tgctcaaaca atagctgctt tatcttcgac agtcttgctc agagcaaacc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 attgttgatc atgtcgtcca ctgaaaaaga gattagtctt ttgaattgta ctttttagaa 4380

4321 taacaactag tacagcaggt gactttttct ctaatcagaa aacttaacat gaaaaatctt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 taatgactca ctgagttgtt gagagagttg agggaactca tagatatgtt cacagttgtt 4440

4381 attactgagt gactcaacaa ctctctcaac tcccttgagt atctatacaa gtgtcaacaa 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcgtgaattc ggaatacaga atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt 4500

4441 agcacttaag ccttatgtct taggcttaag tttcagtttt gtgaagtctt ccgctagaaa 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gaagaagtga cgttcgatca ttcggaaatg atggattggg atgtctccta ctttgaattc 4560

4501 cttcttcact gcaagctagt aagcctttac tacctaaccc tacagaggat gaaacttaag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cacagttgct ccgaccgttt tgagtttcaa aaagtttgga gcaaatctat aacgcacgta 4620

4561 gtgtcaacga ggctggcaaa actcaaagtt tttcaaacct cgtttagata ttgcgtgcat 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcttgccgac tcctgtggcg actcatcatt ctcctgatcg ttctccggtt tggcgatctc 4680

4621 agaacggctg aggacaccgc tgagtagtaa gaggactagc aagaggccaa accgctagag 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gaaaagcact tgctcagtgt ccagatcacg gatttggaac ttggtgaact cgatgttata 4740

4681 cttttcgtga acgagtcaca ggtctagtgc ctaaaccttg aaccacttga gctacaatat 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gatgttcgca gatggggagc ataagaatcc taaatttatg ttttaaactg aaatccaaag 4800

4741 ctacaagcgt ctacccctcg tattcttagg atttaaatac aaaatttgac tttaggtttc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggagcaagat accttgagtg attcccggaa gtgctaaaac gtcgttcgga gtgatttgag 4860

4801 cctcgttcta tggaactcac taagggcctt cacgattttg cagcaagcct cactaaactc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctttcttcgc aagctccgat tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg 4920

4861 gaaagaagcg ttcgaggcta aggcaacact aaggaacaag ccacgaacca ccaccggcac 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcatctggaa atatggaaaa gttcaacaaa aagaaaagag aaaagaatga aatcggatat 4980

4921 cgtagacctt tatacctttt caagttgttt ttcttttctc ttttcttact ttagcctata 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caagagttag ttgagcggtt tctctagttt tctgagtctc acctgcgacg ggaaggtcgc 5040

4981 gttctcaatc aactcgccaa agagatcaaa agactcagag tggacgctgc ccttccagcg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgagccgggt ggaatcgatc gttgttgctc ggctttcata tcggtttggt tggaagcggc 5100

5041 gctcggccca ccttagctag caacaacgag ccgaaagtat agccaaacca accttcgccg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgaaaacgga aagaagtgga agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta 5160

5101 acttttgcct ttcttcacct tcttcctttt ctcacaccac actgtccttt taccattaat 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gagggtgcca aataaccagc tatattttgt ttttttttga aaacattttt aaaaagaaaa 5220

5161 ctcccacggt ttattggtcg atataaaaca aaaaaaaact tttgtaaaaa tttttctttt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atacgataat gatatcagat ggatttccgg aaaactggta tgaaaaattt caaccttttt 5280

5221 tatgctatta ctatagtcta cctaaaggcc ttttgaccat actttttaaa gttggaaaaa 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gagtacatgt aatcaaaata cactttgtaa attatcattt ttattgaaac tccaccattt 5340

5281 ctcatgtaca ttagttttat gtgaaacatt taatagtaaa aataactttg aggtggtaaa 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... SacII(5389) .........

5341 ttctatttat aacgctaata atttgaaaaa gaaacctgtt gcgaaccgcg gggtgaatcc 5400

5341 aagataaata ttgcgattat taaacttttt ctttggacaa cgcttggcgc cccacttagg 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caaaaacgaa tgcgttttgg tggagtgatt gattcgaatc gaagaagaaa aagaagaaga 5460

5401 gtttttgctt acgcaaaacc acctcactaa ctaagcttag cttcttcttt ttcttcttct 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgtggaatag agagctcact cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa 5520

5461 gcaccttatc tctcgagtga gaattggctc gtcgtgtgtg gctgtctttt tttttacttt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgaatgaggg tcttcttctt cttcttcttc gaatgattga cagaaatggg aaaaagagga 5580

5521 acttactccc agaagaagaa gaagaagaag cttactaact gtctttaccc tttttctcct 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agattgagaa gggaaaaagg aaggagaaaa gaagcagaag aagacgtcag agaggagagg 5640

5581 tctaactctt ccctttttcc ttcctctttt cttcgtcttc ttctgcagtc tctcctctcc 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aacgagcgga aaagcagcgg gcgcaagtca tagaagtagc agagctgggg agaagaagac 5700

5641 ttgctcgcct tttcgtcgcc cgcgttcagt atcttcatcg tctcgacccc tcttcttctg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 actatccaag aaaggaatga cgagagagta tgcaaagggg tatagggtgc agacagaata 5760

5701 tgataggttc tttccttact gctctctcat acgtttcccc atatcccacg tctgtcttat 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ggaacagaat aacagatgat gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt 5820

5761 ccttgtctta ttgtctacta ctcggttctt ctcaactttt cccgctactt aaacagtaca 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aacttaattt gggtcaattt gagcatgatg aattgaaatc atcccttgtt gggagttaat 5880

5821 ttgaattaaa cccagttaaa ctcgtactac ttaactttag tagggaacaa ccctcaatta 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aaccggtttg ttatcagaaa ccctgtaata gaagggcgcc ctaactttga gccaattcat 5940

5881 ttggccaaac aatagtcttt gggacattat cttcccgcgg gattgaaact cggttaagta 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cccggtttct gtcaaatata tcaaaaagtg gtcaactgac aaattgtttt tgatattata 6000

5941 gggccaaaga cagtttatat agtttttcac cagttgactg tttaacaaaa actataatat 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ataaacattt tatccgttaa caattttcga atacttttta caaggacttg gataaattgg 6060

6001 tatttgtaaa ataggcaatt gttaaaagct tatgaaaaat gttcctgaac ctatttaacc 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ctcaaagacg aagtgagttt gctaccatca tggaatcagg aggacatttc gacaatgtca 6120

6061 gagtttctgc ttcactcaaa cgatggtagt accttagtcc tcctgtaaag ctgttacagt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tataatgttt gaaaaatgat tttaacaagg gtctcattcg agataagcaa tgctctgagc 6180

6121 atattacaaa ctttttacta aaattgttcc cagagtaagc tctattcgtt acgagactcg 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 aagtggctcg tcgtcttcaa gaatctcttg ttgatattca tttggatcct gttttgcacg 6240

6181 ttcaccgagc agcagaagtt cttagagaac aactataagt aaacctagga caaaacgtgc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aagcatgtgc catggatatt cagagttttt gcaaaaaagt tccgccaggt caaacaatct 6300

6241 ttcgtacacg gtacctataa gtctcaaaaa cgttttttca aggcggtcca gtttgttaga 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ttttaatttt tatatgatac ttcgggtgta tttttcttct ttttcctgtt cattttcgct 6360

6301 aaaattaaaa atatactatg aagcccacat aaaaagaaga aaaaggacaa gtaaaagcga 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tttttctcac ttttttgtaa caaaatctct cacttttctg taagtcttaa caaaagttca 6420

6361 aaaaagagtg aaaaaacatt gttttagaga gtgaaaagac attcagaatt gttttcaagt 6420

.......... .......... .......... .......... .......... ..........

.......... .......... EagI(6444) .......... .......... ..........

6421 attggctcga tcaagtaaaa tttcggccgt atgaacgttt tcaaaggttt tcattttttt 6480

6421 taaccgagct agttcatttt aaagccggca tacttgcaaa agtttccaaa agtaaaaaaa 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ttaagttcat tcacaatatc atgaatctct cgtcctttgg tttccggtaa gtagcgaaat 6540

6481 aattcaagta agtgttatag tacttagaga gcaggaaacc aaaggccatt catcgcttta 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aatataagca tacttataaa tgaaggaatt gaatatagaa taaggaaagc ataactacca 6600

6541 ttatattcgt atgaatattt acttccttaa cttatatctt attcctttcg tattgatggt 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ataacggaat atagaggtaa tactgtaaat gtactgataa ccaccattac agtattcatt 6660

6601 tattgcctta tatctccatt atgacattta catgactatt ggtggtaatg tcataagtaa 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gaatatgcca cagattgagc aatactacga tgtttttgag gtaccagttc agaggatata 6720

6661 cttatacggt gtctaactcg ttatgatgct acaaaaactc catggtcaag tctcctatat 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 aaccacgaaa ttggaccaac tccagttctg aaatttcatt attcgattaa atcaaaatat 6780

6721 ttggtgcttt aacctggttg aggtcaagac tttaaagtaa taagctaatt tagttttata 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 atttgaaaaa cctcacccat aagtataacc gtagatgatc aatgcaacta ggcatccgta 6840

6781 taaacttttt ggagtgggta ttcatattgg catctactag ttacgttgat ccgtaggcat 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 tttcatcggg tcaatcagaa tacgtatttt tgcgaagaat acgaatgcaa ttagagctaa 6900

6841 aaagtagccc agttagtctt atgcataaaa acgcttctta tgcttacgtt aatctcgatt 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SalI(6936) .......... ..........

6901 attattgaga aatgtgaaga gaaggagaag cttgcgtcga ccaactctgt aaacttattc 6960

6901 taataactct ttacacttct cttcctcttc gaacgcagct ggttgagaca tttgaataag 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 atacaatttt gtcttcaaat tttgaacgtt taaaactatc ttattcaatg agttagcggg 7020

6961 tatgttaaaa cagaagttta aaacttgcaa attttgatag aataagttac tcaatcgccc 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tttttatagt gagttagttc acgattatct aataaaacta tgtccaaaca tcctaactgc 7080

7021 aaaaatatca ctcaatcaag tgctaataga ttattttgat acaggtttgt aggattgacg 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gaccgtattc tcactaactc gtcttaaaaa cgttgagatt attttaaacc cttcttcagt 7140

7081 ctggcataag agtgattgag cagaattttt gcaactctaa taaaatttgg gaagaagtca 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 acaactacag tactgatgca caaatacaat aataataata cattcagaca tactctgaac 7200

7141 tgttgatgtc atgactacgt gtttatgtta ttattattat gtaagtctgt atgagacttg 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 aatgtaactt gtttttgtta tactgtggta acatttattg aaatgaaata taaccttact 7260

7201 ttacattgaa caaaaacaat atgacaccat tgtaaataac tttactttat attggaatga 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tttcaatcac aattccacca gtaattgttc cagaaacata agctaaaccc attgcagttg 7320

7261 aaagttagtg ttaaggtggt cattaacaag gtctttgtat tcgatttggg taacgtcaac 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tactccattc agcaatttct gactcaacat ttgcattttc tagaaagaaa gtagaggata 7380

7321 atgaggtaag tcgttaaaga ctgagttgta aacgtaaaag atctttcttt catctcctat 7380

.......... .......... .......... .......... .......... ..........

.......... .......... ApaI(7408) .......... .......... ..........

7381 aaagaagagc ccataatccg acagggccc. .......... .......... .......... 7409

7381 tttcttctcg ggtattaggc tgtcccggg. .......... .......... .......... 7409

.......... .......... .......... .......... .......... ..........