Sequence of pCFJ178

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Sequence of pCFJ178

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Sequence of pCFJ178 - cxTi10882_MCS : 7312 BP; 2130 A; 1450 C; 1666 G; 2066 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 60

1 cgttttctgg tccttggcat ttttccggcg caacgaccgc aaaaaggtat ccgaggcggg 60

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 120

61 gggactgctc gtagtgtttt tagctgcgag ttcagtctcc accgctttgg gctgtcctga 120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 180

121 tatttctatg gtccgcaaag ggggaccttc gagggagcac gcgagaggac aaggctggga 180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 240

181 cggcgaatgg cctatggaca ggcggaaaga gggaagccct tcgcaccgcg aaagagtatc 240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 300

241 gagtgcgaca tccatagagt caagccacat ccagcaagcg aggttcgacc cgacacacgt 300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 360

301 gcttgggggg caagtcgggc tggcgacgcg gaataggcca ttgatagcag aactcaggtt 360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 420

361 gggccattct gtgctgaata gcggtgaccg tcgtcggtga ccattgtcct aatcgtctcg 420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 480

421 ctccatacat ccgccacgat gtctcaagaa cttcaccacc ggattgatgc cgatgtgatc 480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 540

481 ttcctgtcat aaaccataga cgcgagacga cttcggtcaa tggaagcctt tttctcaacc 540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 600

541 atcgagaact aggccgtttg tttggtggcg accatcgcca ccaaaaaaac aaacgttcgt 600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 660

601 cgtctaatgc gcgtcttttt ttcctagagt tcttctagga aactagaaaa gatgccccag 660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 720

661 actgcgagtc accttgcttt tgagtgcaat tccctaaaac cagtactcta atagtttttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 780

721 ctagaagtgg atctaggaaa atttaatttt tacttcaaaa tttagttaga tttcatatat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 840

781 actcatttga accagactgt caatggttac gaattagtca ctccgtggat agagtcgcta 840

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 900

841 gacagataaa gcaagtaggt atcaacggac tgaggggcag cacatctatt gatgctatgc 900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 960

901 cctcccgaat ggtagaccgg ggtcacgacg ttactatggc gctctgggtg cgagtggccg 960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 1020

961 aggtctaaat agtcgttatt tggtcggtcg gccttcccgg ctcgcgtctt caccaggacg 1020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 1080

1021 ttgaaatagg cggaggtagg tcagataatt aacaacggcc cttcgatctc attcatcaag 1080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 1140

1081 cggtcaatta tcaaacgcgt tgcaacaacg gtaacgatgt ccgtagcacc acagtgcgag 1140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 1200

1141 cagcaaacca taccgaagta agtcgaggcc aagggttgct agttccgctc aatgtactag 1200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 1260

1201 ggggtacaac acgttttttc gccaatcgag gaagccagga ggctagcaac agtcttcatt 1260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 1320

1261 caaccggcgt cacaatagtg agtaccaata ccgtcgtgac gtattaagag aatgacagta 1320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 1380

1321 cggtaggcat tctacgaaaa gacactgacc actcatgagt tggttcagta agactcttat 1380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 1440

1381 cacatacgcc gctggctcaa cgagaacggg ccgcagttat gccctattat ggcgcggtgt 1440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 1500

1441 atcgtcttga aattttcacg agtagtaacc ttttgcaaga agccccgctt ttgagagttc 1500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 1560

1501 ctagaatggc gacaactcta ggtcaagcta cattgggtga gcacgtgggt tgactagaag 1560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 1620

1561 tcgtagaaaa tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg ttttacggcg 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 1680

1621 ttttttccct tattcccgct gtgcctttac aacttatgag tatgagaagg aaaaagttat 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 1740

1681 aataacttcg taaatagtcc caataacaga gtactcgcct atgtataaac ttacataaat 1740

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 1800

1741 ctttttattt gtttatcccc aaggcgcgtg taaaggggct tttcacggtg gactgcagat 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 1860

1801 tctttggtaa taatagtact gtaattggat atttttatcc gcatagtgct ccgggaaagc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 1920

1861 agagcgcgca aagccactac tgccactttt ggagactgtg tacgtcgagg gcctctgcca 1920

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 1980

1921 gtgtcgaaca gacattcgcc tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 2040

1981 acaaccgccc acagccccga ccgaattgat acgccgtagt ctcgtctaac atgactctca 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 2100

2041 cgtggtatac gccacacttt atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 2160

2101 ggtaagcggt aagtccgacg cgttgacaac ccttcccgct agccacgccc ggagaagcga 2160

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 2220

2161 taatgcggtc gaccgctttc cccctacacg acgttccgct aattcaaccc attgcggtcc 2220

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa 2280

2221 caaaagggtc agtgctgcaa cattttgctg ccggtcactt aatagttgat acatattatt 2280

lacZ_a M13_pUC_fwd_pri M13_forward20_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2281 agttgcggcc cattaggtca gacaaaaatt tctgatcttt aaaccttatt gattaaaact 2340

2281 tcaacgccgg gtaatccagt ctgtttttaa agactagaaa tttggaataa ctaattttga 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aatttttgcc cgactttttt gacacatgca ctagacgctt gtactcaaaa tttggaaacc 2400

2341 ttaaaaacgg gctgaaaaaa ctgtgtacgt gatctgcgaa catgagtttt aaacctttgg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttgctcaaat ccactactca gatatgacag tagcatcgat aattgaacca ttatcctgaa 2460

2401 aacgagttta ggtgatgagt ctatactgtc atcgtagcta ttaacttggt aataggactt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atcccctttc ctgtcccggt tcattttcca attttttcaa cggtggaaga aggggcaaat 2520

2461 taggggaaag gacagggcca agtaaaaggt taaaaaagtt gccaccttct tccccgttta 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tttataccaa aacgtctccg tttatgaccc accccctatg caaaataaac ggctctggga 2580

2521 aaatatggtt ttgcagaggc aaatactggg tgggggatac gttttatttg ccgagaccct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gaaatagtac agaaaacgcg gagcctgctt gattccttct ccgaggcgac aaaaaacgca 2640

2581 ctttatcatg tcttttgcgc ctcggacgaa ctaaggaaga ggctccgctg ttttttgcgt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcattcgtat tgtaagatgc atgtgtggct ggcctctttt gaattttact ggttttttat 2700

2641 agtaagcata acattctacg tacacaccga ccggagaaaa cttaaaatga ccaaaaaata 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atttctacta atatttcgaa aaattttgta agtaaatcat tgattacgtt tacaaaaatt 2760

2701 taaagatgat tataaagctt tttaaaacat tcatttagta actaatgcaa atgtttttaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tagaaacatt tcaaaatgat tacttctaaa ttaattatat ttacaggtta gttcgagtta 2820

2761 atctttgtaa agttttacta atgaagattt aattaatata aatgtccaat caagctcaat 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cttatgacct tccaatccgc catatccgcc aaggacgctc caattaattt tttagaatta 2880

2821 gaatactgga aggttaggcg gtataggcgg ttcctgcgag gttaattaaa aaatcttaat 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttttttaaca catgtattat tttttaacaa taaattattc atgaaatcat caaatgtata 2940

2881 aaaaaattgt gtacataata aaaaattgtt atttaataag tactttagta gtttacatat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgttctacta taatatttag gtcaaactat agaaaagaaa tccttgtttt tggaacaaag 3000

2941 acaagatgat attataaatc cagtttgata tcttttcttt aggaacaaaa accttgtttc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gagttcagat cctgtgcatt tgatcttgga tccacatctt tggtccatgg gaaagcttgg 3060

3001 ctcaagtcta ggacacgtaa actagaacct aggtgtagaa accaggtacc ctttcgaacc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cactgatcaa gaaggattct tcaaatatct aaaattgccg gctgcccgta gaattccatc 3120

3061 gtgactagtt cttcctaaga agtttataga ttttaacggc cgacgggcat cttaaggtag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agttttccag agttttagtg aaaatttgca tcccgttaga aacaatctac acaacggtaa 3180

3121 tcaaaaggtc tcaaaatcac ttttaaacgt agggcaatct ttgttagatg tgttgccatt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aaaggaccca ttatttttac cgtaccgttt tcctttatgg ccctctatgc aaaaaattat 3240

3181 tttcctgggt aataaaaatg gcatggcaaa aggaaatacc gggagatacg ttttttaata 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agcgtggagg ggcagtacag aataataaag tgtgagaaaa aaaactgctc tgcgagaaat 3300

3241 tcgcacctcc ccgtcatgtc ttattatttc acactctttt ttttgacgag acgctcttta 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agtacagcaa acgcggagca tgcttgattc tatccacttt gtacaagaaa gctgggttaa 3360

3301 tcatgtcgtt tgcgcctcgt acgaactaag ataggtgaaa catgttcttt cgacccaatt 3360

.......... .......... .......... .......... .......... ..........

SpeI(3369) .......... .......... .......... .......... ..........

3361 tacgactcac tagtgggcag atcttcgaat gcatcgcgcg caccgtacgt ctcgaggaat 3420

3361 atgctgagtg atcacccgtc tagaagctta cgtagcgcgc gtggcatgca gagctcctta 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tcctgcagga tatctggatc cacgaagctt cccatggtga cgtcaccggt tctagatacc 3480

3421 aggacgtcct atagacctag gtgcttcgaa gggtaccact gcagtggcca agatctatgg 3480

.......... .......... .......... .......... .......... ..........

.......... KpnI(3497) .... StuI(3510),AflII(3512) .... SmaI(3537)

3481 taggtgagct ctggtaccct ctagtcaagg ccttaagtga gtcgtattac gtagcccggg 3540

3481 atccactcga gaccatggga gatcagttcc ggaattcact cagcataatg catcgggccc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cctagttcta gacattctct aatgaaaaaa tctttcagtt gaaattgaaa atgagttaaa 3600

3541 ggatcaagat ctgtaagaga ttactttttt agaaagtcaa ctttaacttt tactcaattt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gttggagttt ttattgaaaa cagatttccg tgtgattagt gtttttagcg agtgtgacag 3660

3601 caacctcaaa aataactttt gtctaaaggc acactaatca caaaaatcgc tcacactgtc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gacagcgaaa aaatatagaa acaagggggg aactgaaaag cttaggaatg cattgaacat 3720

3661 ctgtcgcttt tttatatctt tgttcccccc ttgacttttc gaatccttac gtaacttgta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gagaagggga aggggaagga acaaactaga caggaattat tggaatttaa tcacatttgg 3780

3721 ctcttcccct tccccttcct tgtttgatct gtccttaata accttaaatt agtgtaaacc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agtttttttt ctattcgaca gaataattat ccagaacatt tttgtattaa atatttatgc 3840

3781 tcaaaaaaaa gataagctgt cttattaata ggtcttgtaa aaacataatt tataaatacg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atcatatgag tagtcggctt tgttgtgcat gacgagtttg ttatcgacga aatagaagct 3900

3841 tagtatactc atcagccgaa acaacacgta ctgctcaaac aatagctgct ttatcttcga 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtcagaacga gtctcgtttg gattgttgat catgtcgtcc actgaaaaag agattagtct 3960

3901 cagtcttgct cagagcaaac ctaacaacta gtacagcagg tgactttttc tctaatcaga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tttgaattgt actttttaga ataatgactc actgagttgt tgagagagtt gagggaactc 4020

3961 aaacttaaca tgaaaaatct tattactgag tgactcaaca actctctcaa ctcccttgag 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atagatatgt tcacagttgt ttcgtgaatt cggaatacag aatccgaatt caaagtcaaa 4080

4021 tatctataca agtgtcaaca aagcacttaa gccttatgtc ttaggcttaa gtttcagttt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 acacttcaga aggcgatctt tgaagaagtg acgttcgatc attcggaaat gatggattgg 4140

4081 tgtgaagtct tccgctagaa acttcttcac tgcaagctag taagccttta ctacctaacc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gatgtctcct actttgaatt ccacagttgc tccgaccgtt ttgagtttca aaaagtttgg 4200

4141 ctacagagga tgaaacttaa ggtgtcaacg aggctggcaa aactcaaagt ttttcaaacc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agcaaatcta taacgcacgt atcttgccga ctcctgtggc gactcatcat tctcctgatc 4260

4201 tcgtttagat attgcgtgca tagaacggct gaggacaccg ctgagtagta agaggactag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gttctccggt ttggcgatct cgaaaagcac ttgctcagtg tccagatcac ggatttggaa 4320

4261 caagaggcca aaccgctaga gcttttcgtg aacgagtcac aggtctagtg cctaaacctt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cttggtgaac tcgatgttat agatgttcgc agatggggag cataagaatc ctaaatttat 4380

4321 gaaccacttg agctacaata tctacaagcg tctacccctc gtattcttag gatttaaata 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gttttaaact gaaatccaaa gggagcaaga taccttgagt gattcccgga agtgctaaaa 4440

4381 caaaatttga ctttaggttt ccctcgttct atggaactca ctaagggcct tcacgatttt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgtcgttcgg agtgatttga gctttcttcg caagctccga ttccgttgtg attccttgtt 4500

4441 gcagcaagcc tcactaaact cgaaagaagc gttcgaggct aaggcaacac taaggaacaa 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cggtgcttgg tggtggccgt ggcatctgga aatatggaaa agttcaacaa aaagaaaaga 4560

4501 gccacgaacc accaccggca ccgtagacct ttataccttt tcaagttgtt tttcttttct 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gaaaagaatg aaatcggata tcaagagtta gttgagcggt ttctctagtt ttctgagtct 4620

4561 cttttcttac tttagcctat agttctcaat caactcgcca aagagatcaa aagactcaga 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cacctgcgac gggaaggtcg ccgagccggg tggaatcgat cgttgttgct cggctttcat 4680

4621 gtggacgctg cccttccagc ggctcggccc accttagcta gcaacaacga gccgaaagta 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atcggtttgg ttggaagcgg ctgaaaacgg aaagaagtgg aagaaggaaa agagtgtggt 4740

4681 tagccaaacc aaccttcgcc gacttttgcc tttcttcacc ttcttccttt tctcacacca 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtgacaggaa aatggtaatt agagggtgcc aaataaccag ctatattttg tttttttttg 4800

4741 cactgtcctt ttaccattaa tctcccacgg tttattggtc gatataaaac aaaaaaaaac 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aaaacatttt taaaaagaaa aatacgataa tgatatcaga tggatttccg gaaaactggt 4860

4801 ttttgtaaaa atttttcttt ttatgctatt actatagtct acctaaaggc cttttgacca 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atgaaaaatt tcaacctttt tgagtacatg taatcaaaat acactttgta aattatcatt 4920

4861 tactttttaa agttggaaaa actcatgtac attagtttta tgtgaaacat ttaatagtaa 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tttattgaaa ctccaccatt tttctattta taacgctaat aatttgaaaa agaaacctat 4980

4921 aaataacttt gaggtggtaa aaagataaat attgcgatta ttaaactttt tctttggata 4980

.......... .......... .......... .......... .......... ..........

SacII(4990) ........ .......... .......... .......... ..........

4981 tgcgaaccgc ggggtgaatc ccaaaaacga atgcgttttg gtggagtgat tgattcgaat 5040

4981 acgcttggcg ccccacttag ggtttttgct tacgcaaaac cacctcacta actaagctta 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgaagaagaa aaagaagaag acgtggaata gagagctcac tcttaaccga gcagcacaca 5100

5041 gcttcttctt tttcttcttc tgcaccttat ctctcgagtg agaattggct cgtcgtgtgt 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccgacagaaa aaaaaatgaa atgaatgagg gtcttcttct tcttcttctt cgaatgattg 5160

5101 ggctgtcttt ttttttactt tacttactcc cagaagaaga agaagaagaa gcttactaac 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 acagaaatgg gaaaaagagg aagattgaga agggaaaaag gaaggagaaa agaagcagaa 5220

5161 tgtctttacc ctttttctcc ttctaactct tccctttttc cttcctcttt tcttcgtctt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gaagacgtca gagaggagag gaacgagcgg aaaagcagcg ggcgcaagtc atagaagtag 5280

5221 cttctgcagt ctctcctctc cttgctcgcc ttttcgtcgc ccgcgttcag tatcttcatc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cagagctggg gagaagaaga cactatccaa gaaaggaatg acgagagagt atgcaaaggg 5340

5281 gtctcgaccc ctcttcttct gtgataggtt ctttccttac tgctctctca tacgtttccc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gtatagggtg cagacagaat aggaacagaa taacagatga tgagccaaga agagttgaaa 5400

5341 catatcccac gtctgtctta tccttgtctt attgtctact actcggttct tctcaacttt 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agggcgatga atttgtcatg taacttaatt tgggtcaatt tgagcatgat gaattgaaat 5460

5401 tcccgctact taaacagtac attgaattaa acccagttaa actcgtacta cttaacttta 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 catcccttgt tgggagttaa taaccggttt gttatcagaa accctgtaat agaagggcgc 5520

5461 gtagggaaca accctcaatt attggccaaa caatagtctt tgggacatta tcttcccgcg 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cctaactttg agccaattca tcccggtttc tgtcaaatat atcaaaaagt ggtcaactga 5580

5521 ggattgaaac tcggttaagt agggccaaag acagtttata tagtttttca ccagttgact 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... HpaI(5619) .......... ..........

5581 caaattgttt ttgatattat aataaacatt ttatccgtta acaattttcg aatacttttt 5640

5581 gtttaacaaa aactataata ttatttgtaa aataggcaat tgttaaaagc ttatgaaaaa 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 acaaggactt ggataaattg gctcaaagag cctgcttttt tgtacaaact tgaattcaaa 5700

5641 tgttcctgaa cctatttaac cgagtttctc ggacgaaaaa acatgtttga acttaagttt 5700

.......... .......... .......... attB1 .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aacgcagaac tcgtactgtt ggatgcctgt gtagcggtcc tctattgatt tttgctattt 5760

5701 ttgcgtcttg agcatgacaa cctacggaca catcgccagg agataactaa aaacgataaa 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tttgataatt ttactgtaaa ttatgagtta ttccaattaa cattcctagc taaatgtaag 5820

5761 aaactattaa aatgacattt aatactcaat aaggttaatt gtaaggatcg atttacattc 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttagcgacca attttttagc aaccccattt tatgactttt cagaatatcg cctactgggt 5880

5821 aatcgctggt taaaaaatcg ttggggtaaa atactgaaaa gtcttatagc ggatgaccca 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 caacgacact tgtttggaac taagggtctt cccaattttt tttaaatctt cataggcttg 5940

5881 gttgctgtga acaaaccttg attcccagaa gggttaaaaa aaatttagaa gtatccgaac 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 acagaatcag ggttcttcaa gcagctttga gagggaatca attgcaacat ttgatgttaa 6000

5941 tgtcttagtc ccaagaagtt cgtcgaaact ctcccttagt taacgttgta aactacaatt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 atttatattt tttatagttc tctggtgaaa tggggaccac cgcaaagccc cttttatgtt 6060

6001 taaatataaa aaatatcaag agaccacttt acccctggtg gcgtttcggg gaaaatacaa 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tttaacttga ataaaatctt gtattaaatt gtgatttata gatctgtatg ggtaatttga 6120

6061 aaattgaact tattttagaa cataatttaa cactaaatat ctagacatac ccattaaact 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 aggaaaagaa agggagctcc caaaagtgcc ttacagatcc aggcccaata aatgtaaact 6180

6121 tccttttctt tccctcgagg gttttcacgg aatgtctagg tccgggttat ttacatttga 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 attgggcagt agtatggcaa aaatcgtgcg gaatttgaaa aacctagaat taaaataaat 6240

6181 taacccgtca tcataccgtt tttagcacgc cttaaacttt ttggatctta attttattta 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 attcaacaca aatgttcgag atattttaag ttcggagtgt atatctagaa catattgtac 6300

6241 taagttgtgt ttacaagctc tataaaattc aagcctcaca tatagatctt gtataacatg 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aagtaggtct acgggatcgt ctacgggaaa atttgatttc ctcgaagatg tatttaatga 6360

6301 ttcatccaga tgccctagca gatgcccttt taaactaaag gagcttctac ataaattact 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 attctctaat gttgcaggtc tttgtatttg acagaatgtt ggtttttgaa acttattcgc 6420

6361 taagagatta caacgtccag aaacataaac tgtcttacaa ccaaaaactt tgaataagcg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cataattgct tggctactac atgtctcatg ccattgacac tatcctgtac tcgaaatttg 6480

6421 gtattaacga accgatgatg tacagagtac ggtaactgtg ataggacatg agctttaaac 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ttaagtaact acccaccgtc cacttatgac gagagggtca attaaggtca ctccacaaaa 6540

6481 aattcattga tgggtggcag gtgaatactg ctctcccagt taattccagt gaggtgtttt 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gtaaatttga aaacacatga gaaaaagtga tctgaattcc cttgaagtgg ctaaaacaaa 6600

6541 catttaaact tttgtgtact ctttttcact agacttaagg gaacttcacc gattttgttt 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gcagatgagg gatacgacgt atgcgactac tgagagataa ggtcgaaact tgataaggat 6660

6601 cgtctactcc ctatgctgca tacgctgatg actctctatt ccagctttga actattccta 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gctgcttacc tgcttagcaa gtttcttcgc acaggagaaa gtgacaagct aaactatcaa 6720

6661 cgacgaatgg acgaatcgtt caaagaagcg tgtcctcttt cactgttcga tttgatagtt 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 attcatctaa aacctcaaaa aattattttg gatgaaataa tgttttattt tgagcaacgc 6780

6721 taagtagatt ttggagtttt ttaataaaac ctactttatt acaaaataaa actcgttgcg 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ataaggcaaa gaataaatta aaggcgatcc gcttagcggc gagttccgtc ctcgaagaag 6840

6781 tattccgttt cttatttaat ttccgctagg cgaatcgccg ctcaaggcag gagcttcttc 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 attcctccat cgatggcgca gtactttgga cagattccac gctctcctgg ggttcctggt 6900

6841 taaggaggta gctaccgcgt catgaaacct gtctaaggtg cgagaggacc ccaaggacca 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ggtccgactg gtcctggctg tcctgggttt ccgtcggctc ctggcttgcc gtctggtcct 6960

6901 ccaggctgac caggaccgac aggacccaaa ggcagccgag gaccgaacgg cagaccagga 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ggtggtccct ttggtccagg ttgtcctgaa agatgtggtg attagatatg gattgaaaaa 7020

6961 ccaccaggga aaccaggtcc aacaggactt tctacaccac taatctatac ctaacttttt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ctatgaaatg attacctggg gttccgtcgt ttcctggttg tccggatggt cctggtggtc 7080

7021 gatactttac taatggaccc caaggcagca aaggaccaac aggcctacca ggaccaccag 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cgactggtcc tggctctccg gtggatcctg gctctccctt ttggactggc tcgcagacag 7140

7081 gctgaccagg accgagaggc cacctaggac cgagagggaa aacctgaccg agcgtctgtc 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ctggaagtcc ctccaacttt ctatacaaag ttgatagctt ggcgtaatca tggtcatagc 7200

7141 gaccttcagg gaggttgaaa gatatgtttc aactatcgaa ccgcattagt accagtatcg 7200

.......... .......... .......... .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

7201 tgtttcctgt gtgaaattgt tatccgctgg tatcagctca ctcaaaggcg gtaatacggt 7260

7201 acaaaggaca cactttaaca ataggcgacc atagtcgagt gagtttccgc cattatgcca 7260

....... M13_pUC_rev_pri ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc ca........ 7312

7261 ataggtgtct tagtccccta ttgcgtcctt tcttgtacac tcgttttccg gt........ 7312

.......... .......... .......... .......... .......... ..........