Sequence of pCFJ68

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Sequence of pCFJ68

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Sequence of pCFJ68: 9549 BP; 2899 A; 1846 C; 1979 G; 2825 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 taaagttgct gtttttgaac acggcgatat gtatctgtag atcatcccca attttcaaca 60

1 atttcaacga caaaaacttg tgccgctata catagacatc tagtaggggt taaaagttgt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atgaatctaa atactgaact ttttgtgtaa tctttagtgc agaaaaatga ccaaaatcag 120

61 tacttagatt tatgacttga aaaacacatt agaaatcacg tctttttact ggttttagtc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgattttccg attataccca aaatatctca gctcaatgag cacctataaa aatgtaaaaa 180

121 gctaaaaggc taatatgggt tttatagagt cgagttactc gtggatattt ttacattttt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 taaagaaagt agtcgaacca tattttgtaa aggctaaaaa actgaataaa tttttatcca 240

181 atttctttca tcagcttggt ataaaacatt tccgattttt tgacttattt aaaaataggt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tccagcgcgg cgttacaatc cgtggcccat gtcacgcacg gctttcaaag aaaaaaaaac 300

241 aggtcgcgcc gcaatgttag gcaccgggta cagtgcgtgc cgaaagtttc tttttttttg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agtctgtttg cgcgacatcg atctggcaaa aatcctaaaa acgactatgc gtggctcact 360

301 tcagacaaac gcgctgtagc tagaccgttt ttaggatttt tgctgatacg caccgagtga 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttatattaat atgaaaaata caaacttggt ggtgcaggtg ctgcagaggc catcgatgaa 420

361 aatataatta tactttttat gtttgaacca ccacgtccac gacgtctccg gtagctactt 420

.......... .......... .......... .......... .......... ..........

StuI(430) .......... .......... .......... .......... ..........

421 gaagtggagg ccttgttgtc gatctacaaa attgtatggt tgtttgagat tttccttgaa 480

421 cttcacctcc ggaacaacag ctagatgttt taacatacca acaaactcta aaaggaactt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 acgctctcaa tcttggatat gatataaatt ctggataaga tatgatggat atgatctatg 540

481 tgcgagagtt agaacctata ctatatttaa gacctattct atactaccta tactagatac 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tttgatagag tgcatttgaa aggataacgg ccctgcagac atttttgaaa aaataacaaa 600

541 aaactatctc acgtaaactt tcctattgcc gggacgtctg taaaaacttt tttattgttt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 catttaattt tctcactcgt ttaggctatt cccctatttt tgatattcct tcgcacatat 660

601 gtaaattaaa agagtgagca aatccgataa ggggataaaa actataagga agcgtgtata 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gaaaactact ttttttcgaa actgttaact ccagaattta taaatctata gcccttactt 720

661 cttttgatga aaaaaagctt tgacaattga ggtcttaaat atttagatat cgggaatgaa 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gattatttat tatcatggtt actcacctca tgtctctttc tcttttcaag aagtcttcgt 780

721 ctaataaata atagtaccaa tgagtggagt acagagaaag agaaaagttc ttcagaagca 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcatatctgt tgtctctttc ctccttttga atctctgctt tgctcttcgc cattgtttcc 840

781 agtatagaca acagagaaag gaggaaaact tagagacgaa acgagaagcg gtaacaaagg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgaaaataat gtaacttgaa ttgtgtaata tactttttta atttgaattt ggcttgtaac 900

841 acttttatta cattgaactt aacacattat atgaaaaaat taaacttaaa ccgaacattg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gcggaatcac tacgtgcggg atcatttctt actagaaaac ccagaaaatg ccatatttca 960

901 cgccttagtg atgcacgccc tagtaaagaa tgatcttttg ggtcttttac ggtataaagt 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cttatctcgg ggtcatttct aattagaaaa gctacaaaaa ccatttctaa ttacggtgac 1020

961 gaatagagcc ccagtaaaga ttaatctttt cgatgttttt ggtaaagatt aatgccactg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tttagaactg gtcgaaatag ctaattagaa atgggttttt gctaattaga ggtgactcta 1080

1021 aaatcttgac cagctttatc gattaatctt tacccaaaaa cgattaatct ccactgagat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgttatctga gtaagcctcg tttgtatgta aactcacagt actaataaat gcaagacacc 1140

1081 acaatagact cattcggagc aaacatacat ttgagtgtca tgattattta cgttctgtgg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgggtttgtc tagatatgaa ataattgaaa tatcaattct gacagacaat aatggtaatc 1200

1141 gcccaaacag atctatactt tattaacttt atagttaaga ctgtctgtta ttaccattag 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttgataagga gttccacgcc caggagaaca cgttagtttt cttgtttttg attgcgtgcg 1260

1201 aactattcct caaggtgcgg gtcctcttgt gcaatcaaaa gaacaaaaac taacgcacgc 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttattttgga gaaaaactcg attttttaca aaataatttt ttgaaaggaa cactgttcaa 1320

1261 aataaaacct ctttttgagc taaaaaatgt tttattaaaa aactttcctt gtgacaagtt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 taagttttgt cttttttctc agttgtgata cggtttttta ttcttttttg tagttataca 1380

1321 attcaaaaca gaaaaaagag tcaacactat gccaaaaaat aagaaaaaac atcaatatgt 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gaagaccgtt acgaaacaga ctgatatcga aacaaacact gatcttactt gcacttacca 1440

1381 cttctggcaa tgctttgtct gactatagct ttgtttgtga ctagaatgaa cgtgaatggt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctttgtacaa gaaagctggg tgacacgcag tttccctcat ttctcatcct tcacgcaata 1500

1441 gaaacatgtt ctttcgaccc actgtgcgtc aaagggagta aagagtagga agtgcgttat 1500

.......... attB2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tttggagact tattctaaac atttccaaaa ccccacggaa atgactatca gtcaacaatt 1560

1501 aaacctctga ataagatttg taaaggtttt ggggtgcctt tactgatagt cagttgttaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgtttattct acgtgcatat catattacca ctatattctg gcaactagca tgaaatttta 1620

1561 acaaataaga tgcacgtata gtataatggt gatataagac cgttgatcgt actttaaaat 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... MscI(1666) ..........

1621 cttcattggg ctcacaatat ggttgcgaca ggatccccgg gattggccaa aggacccaaa 1680

1621 gaagtaaccc gagtgttata ccaacgctgt cctaggggcc ctaaccggtt tcctgggttt 1680

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgagggta 1740

1681 ccatacaaag cttactatga ttgtattgta tcttgtaaaa gtcctcctgg gaactcccat 1740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt 1800

1741 ggccatcttt tttactcatt tcctcttctt gaaaagtgac ctcaacaggg ttaagaacaa 1800

.......... ......... ORF 1 GFP(w/ gaps) ......... GFP_R_pri

.......... .......... .......... .......... .......... ..........

1801 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat 1860

1801 cttaatctac cactacaatt acccgtgttt aaaagacagt cacctctccc acttccacta 1860

GFP_R_pri ... ORF 1 GFP(w/ gaps) GFPfusionREV_pri ... ..........

.......... .......... .......... .......... .......... ..........

1861 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca 1920

1861 cgttgtatgc cttttgaatg ggaatttaaa taaacgtgat gaccttttga tggacaaggt 1920

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca 1980

1921 acccattcaa atttgtatat atatgattga ttgggactaa taaatttaaa agtcggttgt 1980

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... XhoI(2016) .......... ..........

1981 cttgtcacta ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa 2040

1981 gaacagtgat gaaagacaat accacaagtt acgaagagct ctatgggtct agtatacttt 2040

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt 2100

2041 gccgtactga aaaagttctc acggtacggg cttccaatac atgtcctttc ttgatataaa 2100

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca 2160

2101 aagtttctac tgcccttgat gttctgtgca ttcaaatttg tcaagccatg attgattggt 2160

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tacatattta aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga 2220

2161 atgtataaat ttaaaagtcc acgacttcag ttcaaacttc cactatggga acaattatct 2220

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 2280

2221 tagctcaatt ttccataact aaaatttctt ctacctttgt aagaacctgt gtttaacctt 2280

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 2340

2281 atgttgatat tgagtgtgtt acatatgtag taccgtctgt ttgttttctt accttagttt 2340

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gttgtaagtt taaacttgga cttactaact aacggattat atttaaattt tcagaacttc 2400

2341 caacattcaa atttgaacct gaatgattga ttgcctaata taaatttaaa agtcttgaag 2400

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat 2460

2401 ttttaatctg tgttgtaact tctaccttcg caagttgatc gtctggtaat agttgtttta 2460

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct 2520

2461 tgaggttaac cgctaccggg acaggaaaat ggtctgttgg taatggacag gtgtgttaga 2520

.......... ...... ORF 1 GFP(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca 2580

2521 cgggaaagct ttctagggtt gcttttctct ctggtgtacc aggaagaact caaacattgt 2580

.......... ...... ORF 1 GFP(w/ gaps) ...... GFP_F_pri ..........

.......... .......... .......... .......... .......... ..........

2581 gctgctggga ttacacatgg catggatgaa ctatacaaat agcattcgta gaattccaac 2640

2581 cgacgaccct aatgtgtacc gtacctactt gatatgttta tcgtaagcat cttaaggttg 2640

......... GFP(w/ gaps) ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt 2700

2641 actcgcggcc agcgatggta atggttgaac agaccacagt ttttattatc cccggcgaca 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catcagagta agtttaaact gagttctact aactaacgag taatatttaa attttcagca 2760

2701 gtagtctcat tcaaatttga ctcaagatga ttgattgctc attataaatt taaaagtcgt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct 2820

2761 agagcgcggg cacggagact gaagattcag gttaatgaga agttgtaggg atgtacgaga 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt 2880

2821 aagagggaca cgagggtggg ggataaaaac aataatagtt ttttgaagag aattaaagaa 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga 2940

2881 acaaaaaatc gaagaaaatt cagtggagat tgttacttta acacatctaa gtttttatct 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc 3000

2941 taattaagca ttatttttca gcttttttta acacgaggga ggggggtaat tattattaag 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat 3060

3001 atagggtttt agatgtgtta caagacacat gtgaagaata caaaaaatga agactattta 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca 3120

3061 aaaaaacttt gtagtatctt ttttggcgtg tgttttatgg aatagtatac aatgcaaagt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg 3180

3121 caaatactgg cgttaaaaat aaagaagcgt gcagacccgg agagtactgc agtttagtac 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga 3240

3181 gagtagcact ttttcaaaac ctcataaaaa ccttaaaaag ttagttcact ttcaaatact 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg 3300

3241 ttaattaaaa ggacgaaaac gaaaaacccc aaaggggata acaaacagtt ctaaagctcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa 3360

3301 tgccgcaaaa agaacgattt tagtgttcat aactactcgt gctacgttct ttctagcctt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag 3420

3361 cttccaaacc caaactccga gtcaccttcc actcatcttc aactattaaa ctttcacctc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa 3480

3421 atcacagata ccccaaaaac ggaatttact gtcttatgta agggttatat ggtttgtatt 3480

.......... .......... .......... .......... .......... ..........

SpeI(3490) .......... ApaI(3509) .......... .......... ..........

3481 ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt 3540

3481 gacaaaggat gatcagccgg catgcccggg aaagcagagc gcgcaaagcc actactgcca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 3600

3541 cttttggaga ctgtgtacgt cgagggcctc tgccagtgtc gaacagacat tcgcctacgg 3600

.......... pGEX_3_pri .......... .......... .......... ..........

BglII(3601) NotI(3614) ........ .......... .......... ..........

3601 agatctgccc gggcggccgc ctagttctag acattctcta atgaaaaaat ctttcagttg 3660

3601 tctagacggg cccgccggcg gatcaagatc tgtaagagat tactttttta gaaagtcaac 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaattgaaaa tgagttaaag ttggagtttt tattgaaaac agatttccgt gtgattagtg 3720

3661 tttaactttt actcaatttc aacctcaaaa ataacttttg tctaaaggca cactaatcac 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tttttagcga gtgtgacagg acagcgaaaa aatatagaaa caagggggga actgaaaagc 3780

3721 aaaaatcgct cacactgtcc tgtcgctttt ttatatcttt gttcccccct tgacttttcg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttaggaatgc attgaacatg agaaggggaa ggggaaggaa caaactagac aggaattatt 3840

3781 aatccttacg taacttgtac tcttcccctt ccccttcctt gtttgatctg tccttaataa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggaatttaat cacatttgga gttttttttc tattcgacag aataattatc cagaacattt 3900

3841 ccttaaatta gtgtaaacct caaaaaaaag ataagctgtc ttattaatag gtcttgtaaa 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttgtattaaa tatttatgca tcatatgagt agtcggcttt gttgtgcatg acgagtttgt 3960

3901 aacataattt ataaatacgt agtatactca tcagccgaaa caacacgtac tgctcaaaca 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tatcgacgaa atagaagctg tcagaacgag tctcgtttgg attgttgatc atgtcgtcca 4020

3961 atagctgctt tatcttcgac agtcttgctc agagcaaacc taacaactag tacagcaggt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctgaaaaaga gattagtctt ttgaattgta ctttttagaa taatgactca ctgagttgtt 4080

4021 gactttttct ctaatcagaa aacttaacat gaaaaatctt attactgagt gactcaacaa 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagagagttg agggaactca tagatatgtt cacagttgtt tcgtgaattc ggaatacaga 4140

4081 ctctctcaac tcccttgagt atctatacaa gtgtcaacaa agcacttaag ccttatgtct 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt gaagaagtga cgttcgatca 4200

4141 taggcttaag tttcagtttt gtgaagtctt ccgctagaaa cttcttcact gcaagctagt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttcggaaatg atggattggg atgtctccta ctttgaattc cacagttgct ccgaccgttt 4260

4201 aagcctttac tacctaaccc tacagaggat gaaacttaag gtgtcaacga ggctggcaaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgagtttcaa aaagtttgga gcaaatctat aacgcacgta tcttgccgac tcctgtggcg 4320

4261 actcaaagtt tttcaaacct cgtttagata ttgcgtgcat agaacggctg aggacaccgc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actcatcatt ctcctgatcg ttctccggtt tggcgatctc gaaaagcact tgctcagtgt 4380

4321 tgagtagtaa gaggactagc aagaggccaa accgctagag cttttcgtga acgagtcaca 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccagatcacg gatttggaac ttggtgaact cgatgttata gatgttcgca gatggggagc 4440

4381 ggtctagtgc ctaaaccttg aaccacttga gctacaatat ctacaagcgt ctacccctcg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ataagaatcc taaatttatg ttttaaactg aaatccaaag ggagcaagat accttgagtg 4500

4441 tattcttagg atttaaatac aaaatttgac tttaggtttc cctcgttcta tggaactcac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 attcccggaa gtgctaaaac gtcgttcgga gtgatttgag ctttcttcgc aagctccgat 4560

4501 taagggcctt cacgattttg cagcaagcct cactaaactc gaaagaagcg ttcgaggcta 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg gcatctggaa atatggaaaa 4620

4561 aggcaacact aaggaacaag ccacgaacca ccaccggcac cgtagacctt tatacctttt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gttcaacaaa aagaaaagag aaaagaatga aatcggatat caagagttag ttgagcggtt 4680

4621 caagttgttt ttcttttctc ttttcttact ttagcctata gttctcaatc aactcgccaa 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tctctagttt tctgagtctc acctgcgacg ggaaggtcgc cgagccgggt ggaatcgatc 4740

4681 agagatcaaa agactcagag tggacgctgc ccttccagcg gctcggccca ccttagctag 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gttgttgctc ggctttcata tcggtttggt tggaagcggc tgaaaacgga aagaagtgga 4800

4741 caacaacgag ccgaaagtat agccaaacca accttcgccg acttttgcct ttcttcacct 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta gagggtgcca aataaccagc 4860

4801 tcttcctttt ctcacaccac actgtccttt taccattaat ctcccacggt ttattggtcg 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tatattttgt ttttttttga aaacattttt aaaaagaaaa atacgataat gatatcagat 4920

4861 atataaaaca aaaaaaaact tttgtaaaaa tttttctttt tatgctatta ctatagtcta 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggatttccgg aaaactggta tgaaaaattt caaccttttt gagtacatgt aatcaaaata 4980

4921 cctaaaggcc ttttgaccat actttttaaa gttggaaaaa ctcatgtaca ttagttttat 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cactttgtaa attatcattt ttattgaaac tccaccattt ttctatttat aacgctaata 5040

4981 gtgaaacatt taatagtaaa aataactttg aggtggtaaa aagataaata ttgcgattat 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 atttgaaaaa gaaacctgtt gcgaaccgcg gggtgaatcc caaaaacgaa tgcgttttgg 5100

5041 taaacttttt ctttggacaa cgcttggcgc cccacttagg gtttttgctt acgcaaaacc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SacI(5156)

5101 tggagtgatt gattcgaatc gaagaagaaa aagaagaaga cgtggaatag agagctcact 5160

5101 acctcactaa ctaagcttag cttcttcttt ttcttcttct gcaccttatc tctcgagtga 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa tgaatgaggg tcttcttctt 5220

5161 gaattggctc gtcgtgtgtg gctgtctttt tttttacttt acttactccc agaagaagaa 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cttcttcttc gaatgattga cagaaatggg aaaaagagga agattgagaa gggaaaaagg 5280

5221 gaagaagaag cttactaact gtctttaccc tttttctcct tctaactctt ccctttttcc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aaggagaaaa gaagcagaag aagacgtcag agaggagagg aacgagcgga aaagcagcgg 5340

5281 ttcctctttt cttcgtcttc ttctgcagtc tctcctctcc ttgctcgcct tttcgtcgcc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcgcaagtca tagaagtagc agagctgggg agaagaagac actatccaag aaaggaatga 5400

5341 cgcgttcagt atcttcatcg tctcgacccc tcttcttctg tgataggttc tttccttact 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgagagagta tgcaaagggg tatagggtgc agacagaata ggaacagaat aacagatgat 5460

5401 gctctctcat acgtttcccc atatcccacg tctgtcttat ccttgtctta ttgtctacta 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt aacttaattt gggtcaattt 5520

5461 ctcggttctt ctcaactttt cccgctactt aaacagtaca ttgaattaaa cccagttaaa 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gagcatgatg aattgaaatc atcccttgtt gggagttaat aaccggtttg ttatcagaaa 5580

5521 ctcgtactac ttaactttag tagggaacaa ccctcaatta ttggccaaac aatagtcttt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccctgtaata gaagggcgcc ctaactttga gccaattcat cccggtttct gtcaaatata 5640

5581 gggacattat cttcccgcgg gattgaaact cggttaagta gggccaaaga cagtttatat 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tcaaaaagtg gtcaactgac aaattgtttt tgatattata ataaacattt tatccgttaa 5700

5641 agtttttcac cagttgactg tttaacaaaa actataatat tatttgtaaa ataggcaatt 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 caattttcga atacttttta caaggacttg gataaattgg ctcaaagtaa gcctgctttt 5760

5701 gttaaaagct tatgaaaaat gttcctgaac ctatttaacc gagtttcatt cggacgaaaa 5760

.......... .......... .......... .......... .......... attB1

.......... .......... .......... .......... .......... ..........

5761 ttgtacaaac ttgcacgaag tgagtttgct accatcatgg aatcaggagg acatttcgac 5820

5761 aacatgtttg aacgtgcttc actcaaacga tggtagtacc ttagtcctcc tgtaaagctg 5820

attB1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aatgtcatat aatgtttgaa aaatgatttt aacaagggtc tcattcgaga taagcaatgc 5880

5821 ttacagtata ttacaaactt tttactaaaa ttgttcccag agtaagctct attcgttacg 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tctgagcaag tggctcgtcg tcttcaagaa tctcttgttg atattcattt ggatcctgtt 5940

5881 agactcgttc accgagcagc agaagttctt agagaacaac tataagtaaa cctaggacaa 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ttgcacgaag catgtgccat ggatattcag agtttttgca aaaaagttcc gccaggtcaa 6000

5941 aacgtgcttc gtacacggta cctataagtc tcaaaaacgt tttttcaagg cggtccagtt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 acaatctttt taatttttat atgatacttc gggtgtattt ttcttctttt tcctgttcat 6060

6001 tgttagaaaa attaaaaata tactatgaag cccacataaa aagaagaaaa aggacaagta 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tttcgctttt ttctcacttt tttgtaacaa aatctctcac ttttctgtaa gtcttaacaa 6120

6061 aaagcgaaaa aagagtgaaa aaacattgtt ttagagagtg aaaagacatt cagaattgtt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 aagttcaatt ggctcgatca agtaaaattt cggccgtatg aacgttttca aaggttttca 6180

6121 ttcaagttaa ccgagctagt tcattttaaa gccggcatac ttgcaaaagt ttccaaaagt 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttttttttta agttcattca caatatcatg aatctctcgt cctttggttt ccggtaagta 6240

6181 aaaaaaaaat tcaagtaagt gttatagtac ttagagagca ggaaaccaaa ggccattcat 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gcgaaataat ataagcatac ttataaatga aggaattgaa tatagaataa ggaaagcata 6300

6241 cgctttatta tattcgtatg aatatttact tccttaactt atatcttatt cctttcgtat 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 actaccaata acggaatata gaggtaatac tgtaaatgta ctgataacca ccattacagt 6360

6301 tgatggttat tgccttatat ctccattatg acatttacat gactattggt ggtaatgtca 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 attcattgaa tatgccacag attgagcaat actacgatgt ttttgaggta ccagttcaga 6420

6361 taagtaactt atacggtgtc taactcgtta tgatgctaca aaaactccat ggtcaagtct 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ggatataaac cacgaaattg gaccaactcc agttctgaaa tttcattatt cgattaaatc 6480

6421 cctatatttg gtgctttaac ctggttgagg tcaagacttt aaagtaataa gctaatttag 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 aaaatatatt tgaaaaacct cacccataag tataaccgta gatgatcaat gcaactaggc 6540

6481 ttttatataa actttttgga gtgggtattc atattggcat ctactagtta cgttgatccg 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 atccgtattt catcgggtca atcagaatac gtatttttgc gaagaatacg aatgcaatta 6600

6541 taggcataaa gtagcccagt tagtcttatg cataaaaacg cttcttatgc ttacgttaat 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SalI(6643) ..........

6601 gagctaaatt attgagaaat gtgaagagaa ggagaagctt gcgtcgacca actctgtaaa 6660

6601 ctcgatttaa taactcttta cacttctctt cctcttcgaa cgcagctggt tgagacattt 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cttattcata caattttgtc ttcaaatttt gaacgtttaa aactatctta ttcaatgagt 6720

6661 gaataagtat gttaaaacag aagtttaaaa cttgcaaatt ttgatagaat aagttactca 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tagcgggttt ttatagtgag ttagttcacg attatctaat aaaactatgt ccaaacatcc 6780

6721 atcgcccaaa aatatcactc aatcaagtgc taatagatta ttttgataca ggtttgtagg 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 taactgcgac cgtattctca ctaactcgtc ttaaaaacgt tgagattatt ttaaaccctt 6840

6781 attgacgctg gcataagagt gattgagcag aatttttgca actctaataa aatttgggaa 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cttcagtaca actacagtac tgatgcacaa atacaataat aataatacat tcagacatac 6900

6841 gaagtcatgt tgatgtcatg actacgtgtt tatgttatta ttattatgta agtctgtatg 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tctgaacaat gtaacttgtt tttgttatac tgtggtaaca tttattgaaa tgaaatataa 6960

6901 agacttgtta cattgaacaa aaacaatatg acaccattgt aaataacttt actttatatt 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ccttactttt caatcacaat tccaccagta attgttccag aaacataagc taaacccatt 7020

6961 ggaatgaaaa gttagtgtta aggtggtcat taacaaggtc tttgtattcg atttgggtaa 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcagttgtac tccattcagc aatttctgac tcaacatttg cattttctag aaagaaagta 7080

7021 cgtcaacatg aggtaagtcg ttaaagactg agttgtaaac gtaaaagatc tttctttcat 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gaggataaaa gaagagccca taatccgaca agcaactttt ctatacaaag ttgatagctt 7140

7081 ctcctatttt cttctcgggt attaggctgt tcgttgaaaa gatatgtttc aactatcgaa 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctgg tatcagctca 7200

7141 ccgcattagt accagtatcg acaaaggaca cactttaaca ataggcgacc atagtcgagt 7200

....... M13_reverse_pri .... M13_pUC_rev_pri ....... ..........

.......... .......... .......... .......... .......... ..........

7201 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7260

7201 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 agcaaaaggc cagcaaaaga ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7320

7261 tcgttttccg gtcgttttct ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 7320

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

7321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7380

7321 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 7380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7440

7381 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 7440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7500

7441 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 7500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7560

7501 cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 7560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7620

7561 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 7620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7680

7621 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 7680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7740

7681 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 7740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7741 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7800

7741 gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 7800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7860

7801 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa 7860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7920

7861 acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa 7920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7980

7921 aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8040

7981 taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa aatttagtta 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8100

8041 gatttcatat atactcattt gaaccagact gtcaatggtt acgaattagt cactccgtgg 8100

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

8101 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8160

8101 atagagtcgc tagacagata aagcaagtag gtatcaacgg actgaggggc agcacatcta 8160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8220

8161 ttgatgctat gccctcccga atggtagacc ggggtcacga cgttactatg gcgctctggg 8220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8280

8221 tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt cggccttccc ggctcgcgtc 8280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8340

8281 ttcaccagga cgttgaaata ggcggaggta ggtcagataa ttaacaacgg cccttcgatc 8340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8400

8341 tcattcatca agcggtcaat tatcaaacgc gttgcaacaa cggtaacgat gtccgtagca 8400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8460

8401 ccacagtgcg agcagcaaac cataccgaag taagtcgagg ccaagggttg ctagttccgc 8460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8520

8461 tcaatgtact agggggtaca acacgttttt tcgccaatcg aggaagccag gaggctagca 8520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8580

8521 acagtcttca ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg acgtattaag 8580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8640

8581 agaatgacag tacggtaggc attctacgaa aagacactga ccactcatga gttggttcag 8640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8700

8641 taagactctt atcacatacg ccgctggctc aacgagaacg ggccgcagtt atgccctatt 8700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8760

8701 atggcgcggt gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa gaagccccgc 8760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8820

8761 ttttgagagt tcctagaatg gcgacaactc taggtcaagc tacattgggt gagcacgtgg 8820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8880

8821 gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt tttgtccttc 8880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8940

8881 cgttttacgg cgttttttcc cttattcccg ctgtgccttt acaacttatg agtatgagaa 8940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9000

8941 ggaaaaagtt ataataactt cgtaaatagt cccaataaca gagtactcgc ctatgtataa 9000

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

9001 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9060

9001 acttacataa atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9120

9061 tggactgcag attctttggt aataatagta ctgtaattgg atatttttat ccgcatagtg 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9180

9121 ctccgggaaa gcagagcgcg caaagccact actgccactt ttggagactg tgtacgtcga 9180

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

9181 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9240

9181 gggcctctgc cagtgtcgaa cagacattcg cctacggccc tcgtctgttc gggcagtccc 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9300

9241 gcgcagtcgc ccacaaccgc ccacagcccc gaccgaattg atacgccgta gtctcgtcta 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9360

9301 acatgactct cacgtggtat acgccacact ttatggcgtg tctacgcatt cctcttttat 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9420

9361 ggcgtagtcc gcggtaagcg gtaagtccga cgcgttgaca acccttcccg ctagccacgc 9420

.......... .......... .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

9421 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9480

9421 ccggagaagc gataatgcgg tcgaccgctt tccccctaca cgacgttccg ctaattcaac 9480

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattatcaac 9540

9481 ccattgcggt cccaaaaggg tcagtgctgc aacattttgc tgccggtcac ttaatagttg 9540

.......... .... lacZ_a M13_pUC_fwd_pri M13_forward20_pri ......

.......... .......... .......... .......... .......... ..........

9541 tatgtataa. .......... .......... .......... .......... .......... 9549

9541 atacatatt. .......... .......... .......... .......... .......... 9549

.......... .......... .......... .......... .......... ..........